Abstract:
AIMS:Endothelial dysfunction is a key triggering event in the development of cardiovascular diseases and the current study explored this phenomenon in the context of inflammation, apoptosis, reactive oxygen species (ROS) and the phosphatidylinositol 3-kinase (PI3K)/Akt signaling pathway during chronic diabetes. MAIN METHODS:alpha-Lipoic acid (ALA) and wortmannin (WM) were chronically administered to aged Goto Kakizaki (GK) rats, a genetic model of non-obese type II diabetes. Key indices of inflammation, apoptosis and oxidative stress were assessed using western blotting, real-time PCR and immunofluoresence-based techniques. KEY FINDINGS:A chronic inflammation (e.g., increased mRNA/protein levels of TNF-alpha, ICAM, fractalkine, CD-68, myeloperoxidase) in connection with increased caspase-based apoptotic cell death and heightened state of oxidative stress (HSOS)- appear to exist in diabetic cardiovascular tissues. An assessment of NF-kappaB dynamics in aged diabetic vessels revealed not only a marked increase in cytosolic phosphorylated levels of IkappaB-alpha, NIK, IKK but also an enhancement in nuclear localization of p65 concomitantly with augmented NF-kappaB-DNA binding activity. Most of the aforementioned cardiovascular-based diabetic abnormalities including reduced activities of PI3K and Akt kinase were ameliorated following chronic ALA therapy. WM, given to GK rats negated the anti-inflammatory and anti-apoptotic actions of ALA. SIGNIFICANCE:Our data highlight a unifying mechanism whereby HSOS through an induction of NF-kappaB activity together with an impairment in PI3K/Akt pathway favors pro-inflammatory/pro-apoptotic diabetic vascular milieu that culminate in the onset of endothelial dysfunction, a phenomenon which appears to be amenable to treatment with antioxidants and/or PI3/Akt mimetics (e.g., ALA).
journal_name
Life Scijournal_title
Life sciencesauthors
Bitar MS,Ayed AK,Abdel-Halim SM,Isenovic ER,Al-Mulla Fdoi
10.1016/j.lfs.2010.03.019subject
Has Abstractpub_date
2010-06-05 00:00:00pages
844-53issue
23-24eissn
0024-3205issn
1879-0631pii
S0024-3205(10)00142-6journal_volume
86pub_type
杂志文章相关文献
LIFE SCIENCES文献大全abstract::Aspartate (ASP), glutamate (GLU), noradrenaline (NA), dopamine (DA) and its acidic metabolites DOPAC and HVA, serotonin (5-HT) and its metabolite 5-HIAA were simultaneously investigated in post-mortem tissue samples from right parahippocampal gyrus (temporal cortex) and frontal cortical pole (frontal cortex) of adults...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/s0024-3205(97)00067-2
更新日期:1997-01-01 00:00:00
abstract:AIMS:The aim of study is to investigate the effect of nifedipine on viral myocarditis in an animal model. MAIN METHODS:Four-week-old male DBA/2 mice were inoculated with 2 pfu of encephalomyocarditis virus (EMCV) and randomized to nifedipine (n=10) or control (n=10) group. The control group was fed by regular chow and...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/j.lfs.2009.05.018
更新日期:2009-07-31 00:00:00
abstract::Using a dipole tracing method based on the two-dipole model, the purpose of the present study was to investigate alcohol-induced changes in the alpha band of electroencephalogram (EEG) and its equivalent current dipoles (ECDs) in 12 healthy male volunteers, who were genetically typed for mitochondrial aldehyde dehydro...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/s0024-3205(00)00707-4
更新日期:2000-01-01 00:00:00
abstract::In vitro competition binding experiments with the selective muscarinic antagonists AF-DX 116 and pirenzepine (PZ) vs 3H-N-methylscopolamine as radioligand revealed a characteristic distribution of muscarinic receptor subtypes in different regions of rat brain. Based on non linear least squares analysis, the binding da...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/0024-3205(87)90031-2
更新日期:1987-03-02 00:00:00
abstract:AIMS:The central melanocortin system regulates a variety of metabolic functions including lipid metabolism and hepatic lipogenic gene expression. The objective of the present study was to determine whether central melanocortin regulates hepatic lipogenic gene expression under insulin insufficient condition. MAIN METHO...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/j.lfs.2011.01.026
更新日期:2011-04-11 00:00:00
abstract::Insulin therapy remains the standard of care for achieving and maintaining adequate glycemic control, especially in hospitalized patients with critical and noncritical illnesses. Insulin therapy is more effective against elevated fasting glycaemia but less in the reduction of postprandial hyperglycaemia. It is associa...
journal_title:Life sciences
pub_type: 杂志文章,评审
doi:10.1016/j.lfs.2013.12.210
更新日期:2014-03-18 00:00:00
abstract:INTRODUCTION AND AIM:Polycystic ovary syndrome is one of the most common causes of female infertility, affecting 5-10% of the population. Women with PCOS manifest hyperandrogenism, hyperinsulinemia, low-grade systemic inflammation, and polycystic ovaries. Unfortunately, current available medications are only symptomati...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/j.lfs.2019.116640
更新日期:2019-09-01 00:00:00
abstract::Two groups of individuals, 26 normotensive normolipemic and 37 normotensive hyperlipemic, all without family history of hypertension have been selected in attempt to demonstrate whether Li-Na countertransport of erythrocytes is influenced by plasma and membrane lipid composition. The maximal rate of Li-Na countertrans...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/0024-3205(87)90637-0
更新日期:1987-08-31 00:00:00
abstract::Thymol induced superoxide production (O2-) by blood leukocytes was examined in various primates including man. Leukocytes of chimpanzee and hamadryas baboon cells showed only 35% of the maximal O2- production rate obtained in human cells, and those of the Japanese monkey and orang-utan failed to respond. In contrast, ...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/0024-3205(87)90735-1
更新日期:1987-09-28 00:00:00
abstract::In recent years, serum S100B has been used as a secondary endpoint in some clinical trials, in which serum S100B has successfully indicated the benefits or harm done by the tested agents. Compared to clinical stroke studies, few experimental stroke studies report using serum S100B as a surrogate marker for estimating ...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/j.lfs.2007.06.031
更新日期:2007-08-02 00:00:00
abstract::Kainic acid (KA) is a potent convulsant which, when administered subcutaneously, induces sustained limbic seizures and a pattern of limbic brain damage that is thought to be seizure-mediated. Diazepam suppresses and morphine enhances both the seizures and brain damage induced by KA. Here we show that morphine enhancem...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/0024-3205(82)90582-3
更新日期:1982-07-19 00:00:00
abstract::Activation of polyamine metabolism may be important to initiation of pancreatic cell growth. We are reporting that such activation did occur during pancreatic growth initiation by caerulein, a cholecystokinin analog. Maximal increases in total putrescine (319%), spermidine (63%) and spermine (50%) were observed 12, 96...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/0024-3205(84)90456-9
更新日期:1984-12-10 00:00:00
abstract:AIMS:Hypoxia-inducible factor-1α (HIF-1α) is a transcriptional regulator of cellular responses to hypoxic stress. MicroRNAs (miRNAs) play an essential role in hypoxia-mediated cellular responses. Previous studies have identified some let-7 family members as hypoxia-regulated miRNAs (HRMs). In the present study, we aime...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/j.lfs.2017.01.005
更新日期:2017-02-15 00:00:00
abstract:UNLABELLED:Based on preclinical findings, cellular therapy has become a promising therapeutic approach for neonatal hypoxia-ischemia (HI). However, before translation into the clinical setting, new and effective routes of cell delivery must be determined. Intra-arterial (IA) delivery is an attractive route of cellular ...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/j.lfs.2013.10.017
更新日期:2014-02-06 00:00:00
abstract::The Sertoli cells play an essential role in the maintenance and control of spermatogenesis. The ecto-nucleoside triphosphate diphosphohydrolase (E-NTPDase) and 5'-nucleotidase activities can modulate the extracellular adenine nucleotide levels, controlling nucleotide-mediated signaling events in Sertoli cells. Since t...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/j.lfs.2006.08.020
更新日期:2006-12-03 00:00:00
abstract:AIMS:Multiple sclerosis (MS) is one of the commonest neurologic disorders globally. LncRNA OIP5-AS1 has been found to be implicated in the etiology of MS. This study was to explore the roles and molecular mechanisms of OIP5-AS1 in the development of MS. MATERIALS AND METHODS:RT-qPCR assay was used to measure expressio...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/j.lfs.2021.119108
更新日期:2021-01-27 00:00:00
abstract:OBJECTIVE:A deficiency in hydrogen sulfide (H2S) and nitric oxide (NO) contributes to the development of type 2 diabetes (T2D). An inhibitory effect on liver gluconeogenesis has been reported in rats with T2D with co-administration of sodium nitrite and sodium hydrosulfide (NaSH); the underlying mechanisms have however...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/j.lfs.2020.118770
更新日期:2021-01-15 00:00:00
abstract::The release of endogenous dopamine (DA) has been measured in the rat striatum following the intracardial administration of various doses of beta-phenylethylamine (PEA) or alpha,alpha-dideutero-beta-phenylethylamine (deuterated PEA). The release was significantly increased for a period of approximately 15 minutes by a ...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/0024-3205(86)90480-7
更新日期:1986-12-22 00:00:00
abstract::The binding of biologically active 125I-Bolton-Hunter (BH)-NPY to rat brain membranes was saturable and reversible and regulated by inorganic cations and guanyl nucleotides consistent with other neurotransmitter receptor systems. The concentration of specific 125I-NPY binding differed in various brain regions, being h...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/0024-3205(85)90583-1
更新日期:1985-12-02 00:00:00
abstract:AIMS:This study aims to explore factors that influence glucocorticoid (GC)-related genes and receptors/regulatory enzyme expression in intrauterine growth restriction (IUGR) filial rats. MAIN METHODS:An IUGR animal model was established by starvation, and brain tissue was removed after birth. Affymetrix Rat Gene 2.0 S...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/j.lfs.2016.02.079
更新日期:2016-04-01 00:00:00
abstract::Three neurodegenerative diseases, Huntington's disease (HD), Kennedy's disease (hereditary spinobulbar muscular atrophy, SBMA), and type 1 spinocerebellar ataxia (SCA-1) have been found to share a common genetic defect: an unstable region of repeated CAG trinucleotides which are thought to be translated into a polyglu...
journal_title:Life sciences
pub_type: 杂志文章,评审
doi:10.1016/0024-3205(94)90012-4
更新日期:1994-01-01 00:00:00
abstract::In experienced marijuana smokers, marijuana smoking was accompanied by a significant bilateral increase in cerebral blood flow (CBF) especially in the frontal regions and cerebral blood velocity. The post-marijuana CBF increase could not be explained on the basis on changes in general circulation or respiration. Simil...
journal_title:Life sciences
pub_type: 临床试验,杂志文章,随机对照试验
doi:10.1016/0024-3205(93)90239-y
更新日期:1993-01-01 00:00:00
abstract::Hypoxic preconditioning (HPC) has been well demonstrated to have potent protective effects in many cell types; however, the mechanisms responsible for this phenomenon are not fully understood. Recently, glucose-regulated protein 78 (GRP78), an inducible molecular chaperon, was indicated to be associated with ischemic ...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/j.lfs.2007.08.015
更新日期:2007-09-08 00:00:00
abstract::Uncoupling proteins are a family of mitochondrial proteins involved in energy metabolism. We previously showed that uncoupling protein 4 (UCP4) is differentially expressed in omental adipose tissue in diet-induced obese and normal rats. However, the effect of UCP4 on adipocytes is unclear. In this work, we established...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/j.lfs.2006.04.012
更新日期:2006-09-05 00:00:00
abstract::While attempting to delineate the reason for the reported extreme variability of beta-endorphin-like immunoreactivity (beta-ir) in human plasma (eg., nondetectable to 1 ng/ml) by standard radioimmunoassay, we noted that a substantial portion of circulating beta-ir was associated with erythrocytes. That erythrocyte ass...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/0024-3205(84)90677-5
更新日期:1984-05-07 00:00:00
abstract::Arsenic as a one of the most important toxic metals could induce hepatotoxicity. Previous reports revealed the significance of oxidative stress in promoting of arsenic-induced liver toxicity. The aim of the present investigation is to evaluate the effect of chrysin (CHR), a natural flavonoid with potent antioxidant ac...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/j.lfs.2020.118965
更新日期:2021-02-15 00:00:00
abstract::Daily s.c. injection of (-)deprenyl (2.0 mg/kg/day) for three weeks in young male rats caused a threefold increase in superoxide dismutase (SOD) activity in the striatum of the brain compared with the value in saline-injected control rats. Furthermore, the activity of catalase (but not of glutathione peroxidase) was a...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/0024-3205(91)90466-o
更新日期:1991-01-01 00:00:00
abstract:AIMS:Various investigations have demonstrated the protective capacity of general anesthetics as neuroprotective agents. The effects of propofol against ischemia are known to reside in its antioxidant properties and its GABAergic activity. Other aromatic alcohols have also been reported as able to protect neurons agains...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/j.lfs.2017.03.005
更新日期:2017-04-15 00:00:00
abstract:AIMS:Transforming growth factor-β1 (TGF-β1) plays a significant role in epithelial-mesenchymal transition (EMT). Furthermore, endoplasmic reticulum (ER) stress also can induce EMT. However, the relationship among TGF-β1, ER stress and EMT in podocytes is still unclear. Consequently, this study examines the crosstalk ef...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/j.lfs.2018.07.050
更新日期:2018-09-15 00:00:00
abstract::The effects of tetrandrine (TET) on the contractile responses of rat aortic rings and perfused rat mesenteric arteries to phenylephrine (PE) were investigated. TET inhibited the maximal contraction to PE in a concentration-dependent manner. TET significantly inhibited the transient contraction in Ca(2+)-free medium pr...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/s0024-3205(96)00552-8
更新日期:1996-01-01 00:00:00