Abstract:
AIMS:Benidipine is a dihydropyridine (DHP) derived Ca2+ antagonist, can block triple Ca2+ channels (L, N, and T). It has been used as a safety anti-hypertensive drug because of its long-acting relaxant effect on vascular smooth muscle (VSM). However, whether benidipine has similar pharmacological actions in airway smooth muscle (ASM) is unknown. This research aims to reveal the relaxant property and Ca2+ antagonistic effect of benidipine on ASM. MAIN METHODS:The relaxant property of mouse ASM was investigated by tissue tension tests, and Ca2+ antagonistic effect was evaluated through patch-clamp techniques. KEY FINDINGS:Benidipine caused dose-dependent relaxations on high K+ (80 mM) induced precontraction in mouse ASM, which relied on inhibition of extracellular Ca2+ influx, and 1 μM benidipine totally blocked L-type voltage-dependent Ca2+ channels (LVDCCs) currents in airway smooth muscle cells (ASMCs). Benidipine also showed dose-dependent inhibition of ACh-induced precontraction with or without the LVDCCs blocker nifedipine, and 100 μM benidipine blocked ACh-stimulated Ca2+ influx through not only LVDCCs but also non-selective cation channels (NSCCs). SIGNIFICANCE:Benidipine blocked LVDCCs and NSCCs to abolish these channels-mediated Ca2+ influx, which relaxed precontracted ASM. This study represented benidipine with a new potential medicinal value for ASM hypercontractility.
journal_name
Life Scijournal_title
Life sciencesauthors
Yang Y,Chen W,Wen N,Cai C,Liu QH,Shen Jdoi
10.1016/j.lfs.2019.04.036subject
Has Abstractpub_date
2019-06-15 00:00:00pages
74-81eissn
0024-3205issn
1879-0631pii
S0024-3205(19)30298-Xjournal_volume
227pub_type
杂志文章相关文献
LIFE SCIENCES文献大全abstract::Biliary excretion is the main route of disposal of bilirubin and impaired excretion results in jaundice, a well recognisable symptom of liver disease. Conjugation of bilirubin in the liver is essential for its clearance. The glucuronidation of bilirubin is catalysed by the microsomal UDP-glucuronosyltransferase UGT1A1...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/j.lfs.2005.08.018
更新日期:2006-03-06 00:00:00
abstract::Mechanism of Nitric oxide (NO) production by ginsenosides was investigated in cultured porcine endothelial cells. Beta-nicotinamide adenine dinucleotide phosphate (beta-NADPH) staining showed that the NO production was significantly enhanced by the presence of 40 microg/ml ginsenosides with 10 microM L-arginine after ...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/s0024-3205(00)00880-8
更新日期:2000-11-03 00:00:00
abstract::Neurological diseases are becoming increasingly prominent worldwide due to rapidly aging populations, which greatly contributes to increasing healthcare costs. The development of neuroprotective drugs has so far proven exceptionally difficult due to the blood-brain barrier. One novel approach to address this challenge...
journal_title:Life sciences
pub_type: 杂志文章,评审
doi:10.1016/j.lfs.2017.12.025
更新日期:2018-02-15 00:00:00
abstract::Liquid chromatography of porcine duodenal extracts, in conjugation with bioassay on guinea-pig ileum myenteric plexus longitudinal muscle preparation, yielded substances which inhibited electrically-evoked twitches of the preparations, but whose actions could not be reversed by naloxone, an opioid antagonist. The subs...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/0024-3205(84)90439-9
更新日期:1984-12-17 00:00:00
abstract::The ATP-binding cassette (ABC) and solute carrier (SLC) transporter families consist of common drug transporters that mediate the efflux and uptake of drugs, respectively, and play an important role in the absorption, distribution, metabolism and excretion of drugs in vivo. Rheumatoid arthritis (RA) is an autoimmune d...
journal_title:Life sciences
pub_type: 杂志文章,评审
doi:10.1016/j.lfs.2020.118907
更新日期:2021-01-08 00:00:00
abstract::Ether à go-go (EAG) potassium channels possess oncogenic properties and have gained great interest as research tools for cancer detection and therapy. Besides, EAG electrophysiological properties are regulated through the cell cycle and determined by cytoskeletal interactions. Thus, because of the pivotal role of extr...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/j.lfs.2007.05.014
更新日期:2007-06-27 00:00:00
abstract:AIMS:Numerous studies confirmed the involvement of extracellular vesicles in cancer development and progression. The present study was designed to investigate the glycan composition of ectosomes derived by human cutaneous melanoma (CM) cell lines with the use of lectins. MAIN METHODS:Ectosomes released by primary (WM1...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/j.lfs.2018.06.026
更新日期:2018-08-15 00:00:00
abstract:AIMS:This study aims to investigate the value of the expression of miR-208, miR-494, miR-499 and miR-1303 in the early diagnosis of acute myocardial infarction (AMI). MAIN METHODS:Patients were divided into two groups: AMI group (n = 41), and Stable angina pectoris (SAP) group (n = 32). Peripheral venous blood was sam...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/j.lfs.2019.116547
更新日期:2019-09-01 00:00:00
abstract::The effects of 1,3-dipropyl-8(p-sulfophenyl)xanthine (DPSPX) and enprofylline on the respiratory responses to bilateral occlusions of the common carotid arteries (CCO) were studied in rats anesthetized with sodium pentobarbitone and breathing spontaneously. CCO during periods of 5, 10 and 15 s caused increases in tida...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/0024-3205(89)90208-7
更新日期:1989-01-01 00:00:00
abstract::The effects of social crowding in rats on nociception and analgesic actions of mu- and kappa-opioid agonists agonist qualitatively different stimuli were examined. Crowding produced higher control thresholds for electroshock and paw pinch pressure but not for noxious heat. Naloxone did not effect shock thresholds in c...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/0024-3205(82)90345-9
更新日期:1982-09-20 00:00:00
abstract::As a step in the purification and characterization of the glucose transporter from rat skeletal muscle, we have reconstituted glucose transport activity in liposomes. Plasma membranes were prepared from skeletal muscle which display D-glucose reversible binding of cytochalasin B (10 pmol sites/mg protein; KD = 0.3 mic...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/0024-3205(87)90503-0
更新日期:1987-06-15 00:00:00
abstract::Apigenin is one type of bioflavonoid widely found in citrus fruits, which possesses a variety of pharmacological actions on the central nervous system. A previous study showed that acute intraperitoneal administration of apigenin had antidepressant-like effects in the forced swimming test (FST) in ddY mice. To better ...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/j.lfs.2008.01.007
更新日期:2008-03-26 00:00:00
abstract::Daily s.c. injection of (-)deprenyl (2.0 mg/kg/day) for three weeks in young male rats caused a threefold increase in superoxide dismutase (SOD) activity in the striatum of the brain compared with the value in saline-injected control rats. Furthermore, the activity of catalase (but not of glutathione peroxidase) was a...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/0024-3205(91)90466-o
更新日期:1991-01-01 00:00:00
abstract::The effect of naloxone upon neurologic deficit was evaluated in a model of transient forebrain ischemia in rats. Awake male Wistar rats were subjected to a 30 minute ischemia by occluding both common carotid arteries 8 days after cauterizing vertebral arteries. Administration of naloxone 1 or 5 mg/kg iv 10 minutes aft...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/0024-3205(86)90068-8
更新日期:1986-02-03 00:00:00
abstract::Toll-like receptors (TLRs) play important roles in the pathogenesis of atherosclerosis. On the other hand, serum high sensitivity C-reactive protein (hsCRP) is known as an independent coronary risk factor, but cardiovascular events do occur even in low hsCRP levels. We investigated whether the TLR4 expression levels o...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/j.lfs.2006.08.027
更新日期:2006-12-03 00:00:00
abstract::The new Coronavirus (SARS-CoV-2) is the cause of a serious infection in the respiratory tract called COVID-19. Structures of the main protease of SARS-CoV-2 (Mpro), responsible for the replication of the virus, have been solved and quickly made available, thus allowing the design of compounds that could interact with ...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/j.lfs.2020.117963
更新日期:2020-09-01 00:00:00
abstract:AIMS:This study aims to explore the effect and underlying mechanism of zoledronic acid (ZA) on the incidence of thyroid cancer (TC) tumorigenesis. MATERIALS AND METHODS:Human mononuclear cells THP-1 were differentiated into M2-like tumor associated macrophages (TAMs) by incubation with PMA followed by additional incub...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/j.lfs.2020.117925
更新日期:2020-09-01 00:00:00
abstract::DCLHb, a hemoglobin based oxygen carrier, has been extensively studied for the treatment of hemorrhagic shock in both animal models and humans. Numerous accidents resulting in trauma are due to ethanol intoxication, in particular cases of car accidents. Therefore, trauma patients might be intoxicated with drugs of abu...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/s0024-3205(00)01040-7
更新日期:2001-02-09 00:00:00
abstract:AIMS:Thrombin is a serine proteinase that is not only involved in coagulation cascade, but also mediates a number of biological responses relevant to tissues repair, and induces bronchoconstriction. TGF-β plays a pivotal role in airway remodeling due to its effects on airway smooth muscle proliferation and extracellula...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/j.lfs.2018.02.012
更新日期:2018-03-15 00:00:00
abstract::Comparison of neuronal and non-neuronal membrane transport of, and neuroexcitation by, the dicarboxylic amino acids bring out provocative similarities in structural selectivity, and hence in the strategies for studying them. Parallel anomalies in stereoselectivity show for both phenomena that the recognition sites are...
journal_title:Life sciences
pub_type: 杂志文章,评审
doi:10.1016/0024-3205(83)90258-8
更新日期:1983-12-05 00:00:00
abstract:AIMS:Ovarian cancer (OC) is the most lethal gynecologic malignant tumors all over the world. HOX antisense intergenic RNA myeloid 1 (HOTAIRM1) has been reported as an important regulator in multiple tumors. However, the functions of HOTAIRM1 in OC and its possible molecular mechanisms remain unclear. MAIN METHODS:qRT-...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/j.lfs.2020.117296
更新日期:2020-02-15 00:00:00
abstract::Previous behavioral studies on triazolam (TZ), which are small in number, could only speculate about tolerance to the anxiolytic effect of TZ, as the experiments did not cover sufficient time (of 4 to 7 days) for tolerance to develop. Therefore longer time for chronic TZ administration is used. We investigated the eff...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/s0024-3205(03)00612-x
更新日期:2003-10-31 00:00:00
abstract:AIMS:The aim of this study was to investigate the molecular mechanism underlying preterm white matter injury (WMI) via the identification and functional analysis of differentially expressed long non-coding RNAs (lncRNAs) and mRNAs. MAIN METHODS:A neonatal rat model of preterm WMI was established by ligating the common...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/j.lfs.2020.117815
更新日期:2020-08-15 00:00:00
abstract::As the relationship between emotional behavior and electrocardiographic (ECG) change induced by hypothalamic stimulation is poorly understood, eighty-four points in various areas within the hypothalamus in conscious cats were stimulated electrically through chronically implanted electrodes, the objective being to clar...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/0024-3205(85)90494-1
更新日期:1985-03-18 00:00:00
abstract::Human histiocytic lymphoma cells (U-937) undergo similar differentiation when incubated with the phorbol ester 12-0-tetradecanoyl phorbol-13-acetate (TPA) and 1,25-dihydroxycholecalciferol. In this action, TPA somehow implicates calcium-sensitive and phospholipid-dependent protein kinase (protein kinase C), which is r...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/0024-3205(87)90105-6
更新日期:1987-05-25 00:00:00
abstract::Learned helplessness (LH) consists of shock escape deficits evidenced by animals previously exposed to inescapable shock. This phenomenon has shown promise as a behavioral screen for new antidepressant drugs. Unfortunately, some stocks of rats evidence low susceptibility to LH training. Accordingly, male rats from 8 d...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/0024-3205(86)90376-0
更新日期:1986-09-08 00:00:00
abstract:AIMS:The purpose of the study was to determine whether late therapeutic hypothermia (LTH), administered after reperfusion, could prevent adverse left ventricular (LV) remodeling and improve cardiac function in the rat myocardial ischemia/reperfusion model. MAIN METHODS:Rats were randomized to normothermia (n = 10) or ...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/j.lfs.2019.04.026
更新日期:2019-07-15 00:00:00
abstract::Phospholipase C (PLC), an enzyme involved in the hydrolysis of membrane phospholipid- phosphatidylinositol-bisphosphate to inositol triphosphate and diacylglycerol, and Phorbol 12, myristate 13, acetate (PMA), a tumor promoting agent, could significantly stimulate testosterone (T) secretion from Leydig cells. Arachido...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/0024-3205(87)90223-2
更新日期:1987-07-27 00:00:00
abstract:AIM:Up-regulation of inflammasome proteins was reported in dystrophin-deficient muscles. However, it remains to be determined whether inflammasome activation plays a role in the pathogenesis of Duchenne muscular dystrophy. This study was therefore set out to investigate whether genetic disruption of the inflammasome pa...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/j.lfs.2020.118069
更新日期:2020-09-15 00:00:00
abstract::Cancer is a disease of high complexity. Resistance to therapy is a major challenge in cancer targeted therapies. Overcoming this resistance requires a deep knowledge of the cellular interactions within tumor. Natural killer (NK) cells and cytotoxic T lymphocytes (CTLs) are the main anti-cancer immune cells, while T re...
journal_title:Life sciences
pub_type: 杂志文章,评审
doi:10.1016/j.lfs.2019.116960
更新日期:2019-12-01 00:00:00