Abstract：
AIMS:Thrombin is a serine proteinase that is not only involved in coagulation cascade, but also mediates a number of biological responses relevant to tissues repair, and induces bronchoconstriction. TGF-β plays a pivotal role in airway remodeling due to its effects on airway smooth muscle proliferation and extracellular matrix (ECM) deposition. Recently, bronchoconstriction itself is found to constitute a form of strain and is highly relevant to asthmatic airway remodeling. However, the underlying mechanisms remain unknown. Here, we investigated the role of contraction- dependent TGF-β activation in thrombin-induced remodeling in human airway smooth muscle (HASM) cells. MATERIALS AND METHODS:Primary HASM cells were treated with or without thrombin in the absence or presence of anti-TGF-β antibody, cytochalasin D and formoterol. CFSE labeling index or CCK-8 assay were performed to test cell proliferation. RT-PCR and Western blotting were used to examined ECM mRNA level and collagen Iα1, α-actin protein expression, respectively. Immunofluorescence was also used to confirm contraction induced by thrombin in HASM cells. KEY FINDING:Thrombin stimulation enhanced HASM cells proliferation and activated TGF-β signaling. Thrombin induced ECM mRNA and collagen Iα1 protein expression, and these effects are mediated by TGF-β. Abrogation of TGF-β activation by contraction inhibitors cytochalasin D and formoterol prevents the thrombin-induced effects. SIGNIFICANCE:These findings suggest that contraction-dependent TGF-β activation could be a mechanism by which thrombin leads to the development of asthmatic airway remodeling. Blocking physical forces with bronchodilator would be an intriguing way in reducing airway remodeling in asthma.
journal_name
Life Scijournal_title
Life sciencesauthors
Yao ZH,Xie HJ,Yuan YL,Huo YT,Cao J,Lai WY,Cai RJ,Cheng YXdoi
10.1016/j.lfs.2018.02.012subject
Has Abstractpub_date
2018-03-15 00:00:00pages
130-139eissn
0024-3205issn
1879-0631pii
S0024-3205(18)30056-0journal_volume
197pub_type
杂志文章相关文献
LIFE SCIENCES文献大全abstract：AIMS:Tetramethylpyrazine (TMP), one of the active ingredients isolated from a Chinese herbal prescription, possesses protective effects against oxidative stress caused by high glucose in endothelial cells. In this study, the role of TMP in preventing muscle cells from palmitate-induced oxidative damage was investigated...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/j.lfs.2011.02.025
更新日期：2011-04-25 00:00:00
abstract：:Chlordimeform (CDM) and demethylchloridimeform (DCDM) mimic the action of octopamine in elevating adenylate cyclase activity in intact nerve cords of the American cockroach, Periplaneta americana. At a concentration of 1 x 10(-5)M, DCDM (13.5x increase within 20 minutes) is a more potent effector of the response than ...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/0024-3205(83)90644-6
更新日期：1983-06-27 00:00:00
abstract：:In order to explore the effect of neonatal androgen administration on oocyte quality, ovulation induction and in vitro fertilization (IVF) were performed in androgen-sterilized mice. Androgen sterilized mice were produced by the subcutaneous injection of testosterone propionate (TP) at 5 days of age and ovulation indu...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/0024-3205(94)00745-4
更新日期：1994-01-01 00:00:00
abstract：:The effect of clotrimazole on Ca2+ signaling in Madin Darby canine kidney (MDCK) cells was investigated by using fura-2 as a Ca2+ indicator. Clotrimazole (1-30 microM) induced a concentration-dependent [Ca2+]i increase. The [Ca2+]i increase comprised an initial rise and a slow decay. External Ca2+ removal partly inhib...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/s0024-3205(00)00558-0
更新日期：2000-01-01 00:00:00
abstract：:In a partially depolarized guinea pig papillary muscle preparation, BAY K8644 stimulated voltage-operated calcium channels, promoting slow action potentials; this effect was dose-dependent over a concentration range of 3 X 10(-7) M to 3 X 10(-6) M. Isoproterenol and histamine also induced slow action potentials by sti...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/0024-3205(86)90651-x
更新日期：1986-07-28 00:00:00
abstract：:Oxidative stress has been implicated to play an important role in the pathogenesis of diabetic neuropathy, which is the most common complication of diabetes mellitus affecting more than 50% of diabetic patients. In the present study, we have investigated the effect of U83836E [(-)-2-((4-(2,6-Di-1-pyrrolidinyl-4-pyrimi...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/j.lfs.2006.02.033
更新日期：2006-07-17 00:00:00
abstract：:The behavioral response induced by the intravesical instillation of capsaicin has been investigated in catheter-implanted, freely moving, conscious male and female rats. Intravesical instillation of capsaicin (25 nmol/rat in 0.5 ml) evoked an intense licking directed toward the lower abdominal and perineal skin which ...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/0024-3205(94)90054-x
更新日期：1994-01-01 00:00:00
abstract：AIMS:The progressive accumulation of beta-amyloid peptide (Abeta), in the form of senile plaques, has been recognized as one of the major causes of Alzheimer's disease (AD) pathology. Increased production of Abeta and the aggregation of Abeta to oligomers have been reported to trigger neurotoxicity, oxidative damage an...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/j.lfs.2008.12.001
更新日期：2009-02-27 00:00:00
abstract：:The present study was designed to investigate the effects of chronic administration of the alcoholic extract of Terminalia arjuna (TAAE) bark on isoproterenol induced myocardial injury. The TAAE was administered orally to Wistar albino rats (150-200 g) in three different doses, by gastric gavage [3.4 mg/kg: (T1), 6.75...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/s0024-3205(03)00671-4
更新日期：2003-10-10 00:00:00
abstract：:The rate of oxidation of sparteine by the 9000 x g supernatant fraction of a human liver was measured in the presence of various drugs which exert cardiovascular effects. Hexamethonium, ouabain, caffeine and isoproterenol had no effect on this rate, while alprenolol, metoprolol, oxprenolol, propranolol, timolol, pindo...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/0024-3205(84)90332-1
更新日期：1984-01-02 00:00:00
abstract：:The present study analyses the expression and distribution of neuronal nitric oxide synthase (nNOS) in the brainstem of animals pre-treated with Escherichia coli or Helicobacter pylori LPS, at doses that modulate gastric motor function. Systemic administration of H. pylori LPS prevented in a dose-dependent manner (5, ...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/j.lfs.2005.03.007
更新日期：2005-07-15 00:00:00
abstract：OBJECTIVE:Metformin, introduced in 1957, is widely used as an anti-diabetic drug and has considerable benefits in cardiovascular disease reportedly, dependent or independent on its glucose-lowering effects. Aim of this study was to investigate the effect of metformin on gap junction and the inducibility of AF. METHODS...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/j.lfs.2020.117759
更新日期：2020-08-01 00:00:00
abstract：:Adenylate cyclase activity in isolated rat liver plasma membranes was inhibited by NADH in a concentration-dependent manner. Half-maximal inhibition of adenylate cyclase was observed at 120 microM concentration of NADH. The effect of NADH was specific since adenylate cyclase activity was not altered by NAD+, NADP+, NA...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/0024-3205(91)90177-d
更新日期：1991-01-01 00:00:00
abstract：:Binding studies conducted on membrane preparation from human platelets using (3H) Ro5-4864 and (3H) diazepam showed specific and saturable binding. Scatchard analysis revealed a single class of binding sites with KD = 10.8 +/- 0.9 nM and Bmax = 775 +/- 105 fmol/mg protein for (3H) Ro5-4864 and KD = 10.5 +/- 1.1 nM and...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/0024-3205(84)90556-3
更新日期：1984-11-12 00:00:00
abstract：AIMS:Despite the advanced cancer treatments, there is increased resistance to chemotherapy and subsequent mortality. In lack of reliable data in monolayer cultures and animal models, researchers are shifting to 3D cancer spheroids, which represents the in vivo robust tumour morphology. Calcium is essential in cell sign...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/j.lfs.2020.118291
更新日期：2020-11-01 00:00:00
abstract：AIMS:Incidence of stroke increases in postmenopausal women with dangerous consequences. In this study we used zeranol to protect ovariectomized (OVX) rats against cerebral I/R damage and our target is to identify the mechanism of its protection, in addition to investigating whether this mechanism inhibits inflammation ...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/j.lfs.2019.04.035
更新日期：2019-06-15 00:00:00
abstract：:This review aims to systematically review and synthesize scientific evidence for the influence of air pollution exposure and outdoor exercise on health. We conducted a literature search in the PubMed, Cochrane, EMBASE, and Web of Science for articles that evaluated the combination effect of air pollution exposure and ...
journal_title：Life sciences
pub_type： 杂志文章,meta分析
doi：10.1016/j.lfs.2018.12.036
更新日期：2019-02-01 00:00:00
abstract：:The epicardial release of immunoreactive atrial natriuretic peptides (ir-ANPs) in inside-out perfused rabbit atria has been reported. In order to determine the presence of ir-ANPs in pericardial fluid and to evaluate their biochemical characteristics, we measured the concentration of ir-ANPs in pericardial fluid obtai...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/0024-3205(90)90514-r
更新日期：1990-01-01 00:00:00
abstract：:An improved two-dimensional HPTLC procedure was developed for separating phospholipids including individual phosphoinositides, phosphatidic acids and plasmalogens. This procedure was used to examine the time course for uptake of label by phospholipids in brain subcellular membranes after intracerebral injection of [ga...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/0024-3205(89)90475-x
更新日期：1989-01-01 00:00:00
abstract：BACKGROUND:Quercetin is a major flavonoid in various plants. It possesses the multiple pharmacological actions like vascular integrity and regulatory action of the blood-brain barrier. AIMS:The present study is focused on evaluating the role of solid lipid nanoparticle of quercetin in pentylenetetrazole (PTZ) induced ...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/j.lfs.2018.03.010
更新日期：2018-04-15 00:00:00
abstract：:An exclusively milk formula diet stunted the growth of mice immediately following weaning. Milk-fed mice displayed a low-frequency profile of exploratory behavior, while pellet-fed mice showed high-frequency exploration. In contrast to exploratory behavior, feeding behavior did not differ significantly between milk- a...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/j.lfs.2005.04.041
更新日期：2005-11-26 00:00:00
abstract：:The purpose of the this study was to characterize the putative anxiolytic-like effects of the aqueous extract of Albizzia julibrissin stem bark using the elevated plus maze (EPM) in rats. The water extract of Albizzia julibrissin was orally administered at 10, 50, 100 or 200 mg/kg to adult male SD rats, 1 h before beh...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/j.lfs.2004.05.024
更新日期：2004-10-22 00:00:00
abstract：:This paper describes a new formulation for triggered delivery of insulin which consisted of magnetic particles dispersed in alginate spheres. When magnetic field was applied to that system, the release rate of insulin was about 50 times higher than in the absence of magnetic field. The influence of oscillating magneti...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/0024-3205(88)90009-4
更新日期：1988-01-01 00:00:00
abstract：:The low Km GTPase displayed an apparent Km value of 0.2-0.4 microM in P2 fractions from whole mouse brain. The activity of this enzyme ranged from 102 (pmols of GTP hydrolysed per micrograms of protein per min) in the striatum to 39 in the pons-medulla oblongata. Intermediate values were found in other structures, 74-...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/0024-3205(93)90176-4
更新日期：1993-01-01 00:00:00
abstract：:In order to understand the mechanism of increasing body fat in perimenopausal and postmenopausal women, an ovariectomy-induced obesity model was used to study the role of leptin. In this investigation, a long-term study lasted for 13 weeks was conducted to monitoring the change of serum leptin level in rats after the ...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/s0024-3205(99)00181-2
更新日期：1999-01-01 00:00:00
abstract：:The initial stage of atherosclerotic plaque formation involves oxidation of the phosphatidyl-choline moiety of low density lipoprotein (LDL) and subsequent uptake by macrophages. Ongoing uptake in developing plaque also may involve oxidized LDL and would require an oxidizing environment in plaque lipids. Atherosclerot...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/0024-3205(95)02351-8
更新日期：1996-01-01 00:00:00
abstract：:The effects of the mixed class I and II mGLUR agonist (+) 1S,3R-trans-amino-cyclopentane-1,3-dicarboxylic acid (ACPD) and antagonists (+) alpha-methyl-4-carboxyphenylglycine (MCPG) and L-2-amino-3-phosphonopropionic acid (L-AP3) on the basal neuronal excitability and on the expression of in vitro epileptiform activity...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/s0024-3205(97)00941-7
更新日期：1997-01-01 00:00:00
abstract：:Although intraepithelial T lymphocytes of the large intestine (LI) are known to differ from those of the small intestine (SI) in phenotype and function, differences in LI and SI lamina propria (LP) lymphocyte populations have not been clearly established. In this work we found striking phenotypic differences between S...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/j.lfs.2004.08.042
更新日期：2005-04-29 00:00:00
abstract：:The present study was undertaken to examine whether there was a time-dependent change in the toxic effects of amikacin, an aminoglycoside, on renal functions. Male Wistar rats were maintained under conditions of light from 7 am to 7 pm and dark from 7 pm to 7 am. Amikacin (1.2 g/kg) was injected intraperitoneally to a...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/0024-3205(94)00647-4
更新日期：1994-01-01 00:00:00
abstract：AIMS:Histone modifications play central epigenetic roles in regulating the entire genome of the cell and cell proliferation. Herein, we investigated the effects of the natural compound, 16-hydroxycleroda-3,13-dien-15,16-olide (PL3), on the expressions of histone-modifying enzymes, and examined how it induces apoptosis ...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/j.lfs.2011.09.011
更新日期：2011-12-05 00:00:00
