Abstract:
AIM:Up-regulation of inflammasome proteins was reported in dystrophin-deficient muscles. However, it remains to be determined whether inflammasome activation plays a role in the pathogenesis of Duchenne muscular dystrophy. This study was therefore set out to investigate whether genetic disruption of the inflammasome pathway impacts the disease progression in mdx mice. MAIN METHODS:Mice deficient in both dystrophin and ASC (encoded by Pycard [PYD And CARD Domain Containing]) were generated. The impact of ASC deficiency on muscular dystrophy of mdx mice were assessed by measurements of serum cytokines, Western blot, real-time PCR and histopathological staining. KEY FINDINGS:The pro-inflammatory cytokines such as TNF-α, IL-6, KC/GRO and IL-10 were markedly increased in the sera of 8-week-old mdx mice compared to WT. Western blotting showed that P2X7, caspase-1, ASC and IL-18 were upregulated. Disruption of ASC and dystrophin expression in the mdx/ASC-/- mice was verified by Western blot analysis. Histopathological analysis did not find significant alterations in the muscular dystrophy phenotype in mdx/ASC-/- mice as compared to mdx mice. SIGNIFICANCE:Taken together, our results show that disruption of the central adaptor ASC of the inflammasome is insufficient to alleviate muscular dystrophy phenotype in mdx mice.
journal_name
Life Scijournal_title
Life sciencesauthors
Lau YS,Zhao L,Zhang C,Li H,Han Rdoi
10.1016/j.lfs.2020.118069subject
Has Abstractpub_date
2020-09-15 00:00:00pages
118069eissn
0024-3205issn
1879-0631pii
S0024-3205(20)30820-1journal_volume
257pub_type
杂志文章相关文献
LIFE SCIENCES文献大全abstract::The effects of exposure to a schedule of unpredictable cold-immobilization stress on voluntary ethanol consumption were examined. Following testing for ethanol preference, rats were divided into high, medium and low ethanol consuming groups on the basis of daily ethanol intake (g/kg/day) and exposed to immobilization ...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/0024-3205(87)90580-7
更新日期:1987-03-30 00:00:00
abstract::In this study, we have identified the E-NTPDase family members and ecto-5'-nucleotidase/CD73 in rat heart left ventricle. Moreover, we characterize the biochemical properties and enzyme activities from synaptosomes of the nerve terminal endings of heart left ventricle. We observe divalent cation-dependent enzymes that...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/j.lfs.2007.12.003
更新日期:2008-02-27 00:00:00
abstract::Following severe thermal injury, patients are resistant to non-depolarizing muscle relaxants. Although this resistance has been well documented clinically, little is known about its etiology. We have tested the hypothesis that circulating factors contribute to the decreased potency of neuromuscular blockers following ...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/0024-3205(88)90234-2
更新日期:1988-01-01 00:00:00
abstract:AIMS:The steroid and xenobiotic receptor (SXR) regulates the transcription of its target genes by interacting with various nuclear receptor cofactors. We have previously shown that silencing mediator for retinoid and thyroid receptors (SMRT) interacts with SXR even in the presence of rifampicin on cytochrome P450 monoo...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/j.lfs.2013.03.007
更新日期:2013-05-20 00:00:00
abstract::The extended infection with Helicobacter pylori (H. pylori), one of the most frequent infectious agents in humans, may cause gastritis, peptic ulcers, gastric mucosa-associated lymphoid tissue (MALT) lymphoma, and gastric cancer. During H. pylori infection, different kinds of inflammatory cells such as dendritic cells...
journal_title:Life sciences
pub_type: 杂志文章,评审
doi:10.1016/j.lfs.2019.116688
更新日期:2019-08-15 00:00:00
abstract::The multiple functions of the blood-brain barrier (BBB) consist mostly in membrane properties controlling the bidirectional exchange of molecules between the general circulation and the central nervous system. As lipophilic molecules are able to easily penetrate the membrane of brain microvessels endothelial cells, an...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/0024-3205(88)90351-7
更新日期:1988-01-01 00:00:00
abstract:AIMS:Electromagnetic fields (EMFs) can act as inducers or mediators of stress response through the production of heat shock proteins (HSPs) that modulate immune response and thymus functions. In this study, we analyzed cellular stress levels in rat thymus after exposure of the rats to a 2.45 GHz radio frequency (RF) us...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/j.lfs.2015.01.027
更新日期:2015-04-15 00:00:00
abstract::Epidemiological studies have shown that cigarette smoke, an oxidant agent, is a risk factor for the development of diabetic nephropathy (DN), in which pathogenesis transforming growth factor beta(1) (TGFbeta(1)) plays a key role. In our experimental model we exposed mesangial cell cultures to cigarette smoke concentra...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/j.lfs.2003.12.026
更新日期:2004-06-18 00:00:00
abstract::It has been shown that lesions of the medial preoptic/suprachiasmatic nuclei (MPO/SCN) abolish the estrogen-induced afternoon prolactin (PRL) surge. Recent studies using both dopamine antagonist and thyrotropin-releasing hormone (TRH) successfully induced an increase in plasma PRL in various animal models. The same dr...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/s0024-3205(88)80011-0
更新日期:1988-01-01 00:00:00
abstract::Effects of sub-acute cocaine treatment on 3H-naloxone binding to 6 brain regions were examined in spontaneously hypertensive (SHR) and Wistar-Kyoto (WKY) rats. Cocaine hydrochloride (3 mg/kg, i.v.) was given by bolus injection daily for five days. Rats were decapitated 24 hr following the final injection and crude mem...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/0024-3205(88)90421-3
更新日期:1988-01-01 00:00:00
abstract::Despite the recent scientific advances made in cancer diagnostics and therapeutics, cancer still remains the second leading cause of death worldwide. Thus, there is a need to identify new potential biomarkers/molecular targets to improve the diagnosis and treatment of cancer patients. In this regard, long non-coding R...
journal_title:Life sciences
pub_type: 杂志文章,评审
doi:10.1016/j.lfs.2020.118035
更新日期:2020-09-15 00:00:00
abstract::Using liposomes differing in size and lipid composition, we have studied the uptake characteristics of the liver parenchymal and Kupffer cells. Desferal labeled with iron-59 was chosen as a radiomarker for the liposomal content, because Desferal in its free form does not cross cellular membranes. At various time inter...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/0024-3205(82)90098-4
更新日期:1982-11-08 00:00:00
abstract::The epicardial release of immunoreactive atrial natriuretic peptides (ir-ANPs) in inside-out perfused rabbit atria has been reported. In order to determine the presence of ir-ANPs in pericardial fluid and to evaluate their biochemical characteristics, we measured the concentration of ir-ANPs in pericardial fluid obtai...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/0024-3205(90)90514-r
更新日期:1990-01-01 00:00:00
abstract::Exposure to Cd(2+) via inhalation or intratracheal instillation results in pulmonary edema, which is followed by the influx of leukocytes, the proliferation of type II pneumocytes and eventual scarring and fibrotic changes. While the general toxic effects of Cd(2+) in the lung have been well characterized, the specifi...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/s0024-3205(02)02379-2
更新日期:2003-01-31 00:00:00
abstract:AIMS:To investigate the effects of aerobic exercise training on cardiomyocyte ultrastructure, oxidative stress, and activation of protein synthesis pathways in a model of cardiomyopathy induced by doxorubicin (Dox). MAIN METHODS:Male Sprague Dawley rats were randomly assigned to Control (saline, sedentary), Dox/sedent...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/j.lfs.2020.118698
更新日期:2021-01-01 00:00:00
abstract::Previous research has shown that the CAMK (calcium/calmodulin dependent protein kinase) inhibitor, KN62, can lead to reductions in insulin stimulated glucose transport. Although controversial, an L-type calcium channel mechanism has also been hypothesized to be involved in insulin stimulated glucose transport. The pur...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/j.lfs.2003.06.041
更新日期:2004-01-02 00:00:00
abstract:AIMS:Hyperhomocysteinemia (Hhcy) is an independent risk factor of atherosclerosis and promotes unstable plaque formation. Epigenetic mechanisms play an important role in the pathogenesis of atherosclerosis induced by Hhcy. However, the exact mechanism is still undefined. Lesional apoptotic cells and necrotic core forma...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/j.lfs.2017.02.003
更新日期:2017-03-15 00:00:00
abstract::Mucosal mast cells are implicated in visceral hypersensitivity associated with irritable bowel syndrome (IBS). In this study, we investigated the role of mast cells in the development of visceral hypersensitivity by using mast cell deficient (Ws/Ws) rats and their control (W+/W+). In W+/W+ rats, an injection of 2,4,6-...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/j.lfs.2007.11.027
更新日期:2008-02-13 00:00:00
abstract::Though chemokines of the CXC family are thought to play key roles in neoplastic transformation and tumor invasion, information about CXC chemokines in prostate cancer is sparse. To evaluate the involvement of CXC chemokines in prostate cancer, we analyzed the CXC coding mRNA of both chemokine ligands (CXCL) and chemok...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/j.lfs.2005.08.019
更新日期:2006-03-13 00:00:00
abstract::1-oleoyl-2-acetyl glycerol (OAG), a potent activator of protein kinase C, inhibited the binding of 125I-labelled epidermal growth factor (EGF) in isolated rat pancreatic acini. Unlike cholecystokinin-octapeptide (CCK8) and the C-kinase activator 12-O-tetradecanoyl phorbol-13-acetate (TPA), two inhibitors of 125I-EGF e...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/0024-3205(87)90129-9
更新日期:1987-04-20 00:00:00
abstract::The effects of aporphines and secoaporphines on glucose uptake by isolated intestinal brush-border membrane vesicles (BBMV) or basolateral membrane vesicles (BLMV) and glucose absorption during in situ intestinal perfusion were studied. Of the tested compounds, N-allylsecoboldine was the most potent glucose uptake inh...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/j.lfs.2005.12.031
更新日期:2006-06-06 00:00:00
abstract::Recently, 4.4'-bis(1-p-carboxyphenyl-3-methyl-5-dydroxyl)-pyrazol (DRD156) has been developed as a new sensitive reagent that reacts specifically with singlet oxygen. The specificity of DRD156 for singlet oxygen in a biomimetic solution (micellar solution) and the effects of its coexistence with other reagent were exa...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/s0024-3205(02)02234-8
更新日期:2002-12-06 00:00:00
abstract::In order to determine the effect of relaxin and estradiol on collagen and noncollagen synthesis by mammary gland fibroblasts, fibroblasts isolated from guinea pig mammary glands were grown on plastic or Cytodex-3 collagen coated microcarriers. On plastic, estradiol (600 pg/ml) increased the incorporation of tritiated ...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/0024-3205(84)90460-0
更新日期:1984-11-26 00:00:00
abstract::Twice daily injection of caffeine (30 mg/kg) for 3-1/2 days shifted the caffeine stimulus generalization curve to the right by 3-fold in rats trained to discriminate saline from 30 mg/kg of caffeine, and by 4-fold in rats trained to discriminate saline from 10 mg/kg of caffeine. The latter group was also tested for cr...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/0024-3205(87)90140-8
更新日期:1987-01-26 00:00:00
abstract::Although the aging effect of dopamine D2 receptor in the striatum is well-documented, the effect of age on the extrastriatal dopamine D2 receptor has not been fully examined. Since the density of extrastriatal dopamine D2 receptor is very low, suitable ligands are limited. In this study, we used [11C]FLB 457 to quanti...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/s0024-3205(01)01205-x
更新日期:2001-07-20 00:00:00
abstract:AIM:The aim of the present study was to assess and compare the effect of 17β-estradiol and two different selective estrogen receptor modulators (SERMs), tamoxifen and raloxifene, as well as a selective estrogen receptor alpha agonist, propyl-pyrazole-triol (PPT) and a selective estrogen receptor beta agonist, diarylpro...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/j.lfs.2011.03.010
更新日期:2011-05-09 00:00:00
abstract:AIM:To investigate the cytotoxic effect of Peruvoside and mechanism of action in human cancers. MAIN METHODS:Cell viability was measured by MTT assay and the cell cycle arrest was identified by FACS. Real-time qPCR and western blotting studies were performed to identify important gene and protein expressions in the di...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/j.lfs.2019.117147
更新日期:2020-01-15 00:00:00
abstract::Heart rate (HR) and mean arterial blood pressure (BP) were recorded from conscious, chair-restrained squirrel monkeys surgically prepared with chronically indwelling arterial and venous catheters to determine the effects of acute intravenous injections of two opiate antagonists and an agonist. Naloxone (0.3--10.0 mg/k...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/0024-3205(83)90085-1
更新日期:1983-01-24 00:00:00
abstract:AIMS:Few studies have compared the interaction of single and repeated administration of amitriptyline (amit) with the nitrergic system and glutamatergic system in the experimental model of neuropathic pain. We aimed to evaluate the antinociceptive effect of single and repeated administration of amit and to assess wheth...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/j.lfs.2019.116752
更新日期:2019-09-15 00:00:00
abstract::The feasibility of visualizing the heterogeneity of benzodiazepine (BDZ) receptors in the brain of living baboons was investigated using Positron Emission Tomography. Ethyl 8-fluoro-5,6-dihydro-5-methyl 6-oxo-4H-imidazo (1,5-a) (1, 4) benzodiazepine-3-carboxylate (RO 15 1788) labelled by carbon 11 (11C-RO 15 1788) was...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/0024-3205(85)90363-7
更新日期:1985-04-29 00:00:00