Abstract:
:Mucosal mast cells are implicated in visceral hypersensitivity associated with irritable bowel syndrome (IBS). In this study, we investigated the role of mast cells in the development of visceral hypersensitivity by using mast cell deficient (Ws/Ws) rats and their control (W+/W+). In W+/W+ rats, an injection of 2,4,6-trinitrobenzene sulfonic acid (TNBS) into the proximal colon produced a significant decrease in pain threshold of the distal colon. Severe mucosal necrosis and inflammatory cell infiltration with concomitant increase in tissue myeloperoxidase activity were observed in the proximal colon that was directly insulted by TNBS, whereas neither necrosis nor increased myeloperoxidase activity occurred in the distal colon, indicating that TNBS-induced hypersensitivity is not caused by the local tissue damage or inflammation in the region of the gut where distention stimuli were applied. On the other hand, TNBS failed to elicit visceral hypersensitivity in Ws/Ws rats. This finding indicates that mast cells are essential for development of TNBS-induced visceral hypersensitivity in rats. Since the severity of TNBS-induced proximal colon injury and MPO activity was not affected by mast cell deficiency, it is unlikely that abolishment of visceral hypersensitivity in mast cell deficient rats was a result of altered development of the primary injury in the proximal colon. There was no difference between sham-operated Ws/Ws and W+/W+ rats in colonic pain threshold to distention stimuli, indicating that mast cells play no modulatory roles in normal colonic nociception. The present results support the view that mucosal mast cells play key roles in the pathogenesis of IBS.
journal_name
Life Scijournal_title
Life sciencesauthors
Ohashi K,Sato Y,Kawai M,Kurebayashi Ydoi
10.1016/j.lfs.2007.11.027subject
Has Abstractpub_date
2008-02-13 00:00:00pages
419-23issue
7-8eissn
0024-3205issn
1879-0631pii
S0024-3205(07)00846-6journal_volume
82pub_type
杂志文章相关文献
LIFE SCIENCES文献大全abstract::The Sertoli cells play an essential role in the maintenance and control of spermatogenesis. The ecto-nucleoside triphosphate diphosphohydrolase (E-NTPDase) and 5'-nucleotidase activities can modulate the extracellular adenine nucleotide levels, controlling nucleotide-mediated signaling events in Sertoli cells. Since t...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/j.lfs.2006.08.020
更新日期:2006-12-03 00:00:00
abstract::Extracts of Hypericum perforatum (St. John's wort) have antidepressant properties in depressed patients and exert antidepressant-like action in laboratory animals. The phloroglucinol derivative hyperforin has become a topic of interest, as this Hypericum component is a potent inhibitor of monoamines reuptake. The mole...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/s0024-3205(03)00295-9
更新日期:2003-06-13 00:00:00
abstract::High performance liquid chromatography combined with either single quad or triple quad mass spectral detectors (LC/MS) was used to measure the brain distribution of receptor occupancy tracers targeting dopamine D2, serotonin 5-HT2A and neurokinin NK-1 receptors using the ligands raclopride, MDL-100907 and GR205171, re...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/j.lfs.2005.04.075
更新日期:2005-12-12 00:00:00
abstract:AIMS:Insulinoma-associated protein 2β (IA-2β) is considered to play a significant role in regulated secretion. Recent studies have shown that the mouse brain expresses three major isoforms of IA-2β, named IA-2β60, IA-2β64, and IA-2β71. In this study, we analyzed the tissue-, cell- and organelle-specific distributions o...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/j.lfs.2011.02.018
更新日期:2011-04-25 00:00:00
abstract::Early induction of VEGF was studied in liver, kidney and lung of spontaneously diabetic rats. Western blot analysis, northern hybridization were applied to show the expression of VEGF in different organs. Radiolabelled hypoxia responsive element (HRE) and cAMP responsive element (CRE) oligonucleotides were assayed by ...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/s0024-3205(01)01327-3
更新日期:2001-10-12 00:00:00
abstract:AIM:Ethanol is one of the most widely used recreational drugs in the world. At high concentrations, it can induce carotid artery vasoconstriction. Hyperthermia potentiates its effects resulting in carotid artery vasoconstriction at any concentration. The aim of this study is to investigate the interaction between ethan...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/j.lfs.2017.08.037
更新日期:2017-11-01 00:00:00
abstract:AIMS:Electromagnetic fields (EMFs) can act as inducers or mediators of stress response through the production of heat shock proteins (HSPs) that modulate immune response and thymus functions. In this study, we analyzed cellular stress levels in rat thymus after exposure of the rats to a 2.45 GHz radio frequency (RF) us...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/j.lfs.2015.01.027
更新日期:2015-04-15 00:00:00
abstract::We hypothesized that the atheroinhibitory and cardioprotective effects of estrogen may be mediated in part through increased prostacyclin formation by the artery wall. Atherosclerotic abdominal aorta was collected at necropsy from ovariectomized female monkeys fed an atherogenic diet alone or with added Premarin. Basa...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/s0024-3205(01)01131-6
更新日期:2001-06-15 00:00:00
abstract::Flavonoids from the leaves of Diospyros kaki L (FLDK-P70) are main therapeutic components of NaoXingQing, which is a novel and patented Traditional Chinese Medicine drug used for the treatment of syndrome of apoplexy for years in China. The present study investigated the effects of FLDK-P70 on hydrogen peroxide (H2O2)...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/j.lfs.2004.09.031
更新日期:2005-03-11 00:00:00
abstract::Previous behavioral studies on triazolam (TZ), which are small in number, could only speculate about tolerance to the anxiolytic effect of TZ, as the experiments did not cover sufficient time (of 4 to 7 days) for tolerance to develop. Therefore longer time for chronic TZ administration is used. We investigated the eff...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/s0024-3205(03)00612-x
更新日期:2003-10-31 00:00:00
abstract::The Ca2+ ionophore A23187 (0.2-5 microM) stimulates the phosphorylation of the substrates of protein kinase C (40,000 dalton protein) and myosin light chain kinase (20,000 dalton protein) in the presence or absence of cyclooxygenase inhibitors. In the presence of cyclooxygenase inhibitors or millimolar Ca2+ there is n...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/0024-3205(86)90024-x
更新日期:1986-08-25 00:00:00
abstract::The object of this study was to develop an assay for platelet activating factor (PAF) in rat plasma, and to utilise this to determine the effects of dietary fish oil on PAF in normotensive and spontaneously hypertensive rats. Measurement of platelet activating factor in blood plasma has proved difficult because of its...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/0024-3205(86)90143-8
更新日期:1986-05-19 00:00:00
abstract:AIMS:Pulmonary hypertension (PH) is a proliferative disorder characterized by enhanced proliferation and suppressed apoptosis of intrapulmonary vascular smooth muscle cells. Recently, network-based bioinformatics have identified let-7 family, a tumor suppressive microRNA, regulate multiple interacting targets relevant ...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/j.lfs.2016.11.020
更新日期:2017-02-01 00:00:00
abstract::There is now some evidence that i) the availability of plasma tryptophan, the precursor of serotonin, is significantly lower in pregnant women at the end of term and the first few days after delivery than in nonpregnant women; and ii) both pregnancy and the early puerperium are accompanied by activation of the inflamm...
journal_title:Life sciences
pub_type: 临床试验,杂志文章
doi:10.1016/s0024-3205(02)01853-2
更新日期:2002-09-06 00:00:00
abstract::Immunoreactive- (IR-) somatostatin (SRIF), neuropeptide Y (NPY) and corticotropin-releasing factor (CRF) contents were investigated in the brain of tremor rats with absence-like seizure and spontaneously epileptic rats (SER), which is a genetically defined double-mutant (zi/zi, tm/tm) obtained by mating zitter homozyg...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/0024-3205(95)00302-m
更新日期:1995-01-01 00:00:00
abstract:AIMS:Fenofibrate (FF) is commonly used clinically as a lipid-lowering drug, but whether it participates in endoplasmic reticulum (ER) stress and decreases inflammation in skeletal muscle is still unknown. The aim of this study is to determine whether FF treatment reduces insulin resistance (IR) by alleviating ER stress...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/j.lfs.2016.06.008
更新日期:2016-07-15 00:00:00
abstract:BACKGROUND AND AIMS:Dental pulp stem cells (DPSC) are promising tools in regenerative medicine due to their differentiation potential and immunomodulatory properties. However, it is not clearly known whether or not DPSCs maintain their immunosuppressive effects after differentiation. In the present study, we examined t...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/j.lfs.2018.11.040
更新日期:2019-01-01 00:00:00
abstract::Although both the incidence and the mortality rate of breast cancer is rising, there is no potent and practical option for the treatment of these patients, particularly in advanced stages. One of the most critical challenges for treatment is the presence of complicated and extensive tumor escape mechanisms in the tumo...
journal_title:Life sciences
pub_type: 杂志文章,评审
doi:10.1016/j.lfs.2020.117437
更新日期:2020-04-15 00:00:00
abstract:AIMS:This study aims to explore the effect and underlying mechanism of zoledronic acid (ZA) on the incidence of thyroid cancer (TC) tumorigenesis. MATERIALS AND METHODS:Human mononuclear cells THP-1 were differentiated into M2-like tumor associated macrophages (TAMs) by incubation with PMA followed by additional incub...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/j.lfs.2020.117925
更新日期:2020-09-01 00:00:00
abstract::Cumulative damage in cells from aged people could lead to a greater fragility against acute oxidative stress. The effects of acute oxidative stress on cell viability, cAMP and cGMP concentrations, and protein synthesis rates were studied in lymphocytes from 25 young and 26 elderly subjects. Lymphocytes were exposed to...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/s0024-3205(98)00231-8
更新日期:1998-01-01 00:00:00
abstract::Six diterpenes (three clerodanes, two abietanes and one rosane) were tested for interactions with the cyclooxygenase and 5-lipoxygenase pathways of arachidonate metabolism and for effects of nitric oxide production. Two abietane diterpenes, aethiopinone and 11,12-dihydroxy-6-oxo-8,11,13-abietatriene and the rosane lag...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/s0024-3205(01)01402-3
更新日期:2001-12-07 00:00:00
abstract::Alterations in the cyclic AMP-dependent protein kinase activity ratio in response to putative neurotransmitters and other cyclic AMP-elevating agents in intact cerebral cortical slices and Krebs-Ringer particulate preparations from cerebral cortex were examined. Both norepinephrine (30 microM) and forskolin (20 microM...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/0024-3205(85)90629-0
更新日期:1985-07-08 00:00:00
abstract::The mouse neuroblastoma x rat glioma hybrid NG108-15 was previously shown to express delta opioid receptors. Because neuroblastoma cells display different phenotypes and cloned cell lines are heterogenous, we studied the characteristics and distribution of human 125I-beta-endorphin (125I-beta E) binding sites in cultu...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/0024-3205(92)90306-a
更新日期:1992-01-01 00:00:00
abstract::[3H]-Spiroperidol specifically binds at sites in human prefrontal cortex. The binding of this ligand is apparently anomalous at 37 degrees C, with a substantial loss of specific binding occurring between 5 and 40 min incubation. However, at 21 degrees C, this loss of binding is not observed even at 60 min. At 21 degre...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/0024-3205(84)90282-0
更新日期:1984-06-18 00:00:00
abstract::The relative distribution of muscarinic and nicotinic cholinergic receptors labeled with [3H]acetylcholine was determined using autoradiography. [3H]Acetylcholine binding to high affinity muscarinic receptors was similar to what has been described for an M-2 distribution: highest levels of binding occurred in the pont...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/0024-3205(86)90210-9
更新日期:1986-06-09 00:00:00
abstract:AIMS:Reactive oxygen species (ROS) are generated in the ischaemic myocardium especially during early reperfusion and affect myocardial function and viability. Monoterpenes have been proposed to play beneficial roles in diverse physiological systems; however, the mechanisms of action remain largely unknown. This study a...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/j.lfs.2017.01.008
更新日期:2017-03-01 00:00:00
abstract::Male Sprague-Dawley rats were castrated and given daily sc. injections of estradiol (E2, 10 micrograms/day), testosterone propionate (TP, 1.0 mg/day), dihydrotestosterone (DHT, 1.0 mg/day) or sesame oil (SO, 0.2 ml/day). A group of sham castrate males received daily sc. injections of SO (0.2 ml/day). On day 8 of stero...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/0024-3205(84)90327-8
更新日期:1984-01-02 00:00:00
abstract::The composition of various amino acids and related compounds in the aorta, ventricle, atria, liver, kidney, pancreas, bronchi and adrenals of rats is presented. These patterns are qualitatively similar, but quantitatively different. Stress changed these patterns. In the aorta, alpha-aminobutyric acid and ammonia are d...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/0024-3205(95)02156-d
更新日期:1995-01-01 00:00:00
abstract::Daily s.c. injection of (-)deprenyl (2.0 mg/kg/day) for three weeks in young male rats caused a threefold increase in superoxide dismutase (SOD) activity in the striatum of the brain compared with the value in saline-injected control rats. Furthermore, the activity of catalase (but not of glutathione peroxidase) was a...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/0024-3205(91)90466-o
更新日期:1991-01-01 00:00:00
abstract::L-Histidine, histamine ivc, and amodiaquine (inhibitor of histamine catabolism) enhanced catalepsy induced by morphine, codeine and fentanyl. Haloperidol catalepsy was increased only by ivc histamine. H1 (but not H2) receptor antagonists have shown anticataleptic effect in the action of analgesics and haloperidol. ...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/0024-3205(83)90580-5
更新日期:1983-01-01 00:00:00