Abstract:
:Early induction of VEGF was studied in liver, kidney and lung of spontaneously diabetic rats. Western blot analysis, northern hybridization were applied to show the expression of VEGF in different organs. Radiolabelled hypoxia responsive element (HRE) and cAMP responsive element (CRE) oligonucleotides were assayed by electrophoretic mobility shift (EMSA) or supershift using anti ARNT and anti CREB-1 monoclonal antibodies. An increase in VEGF expression at the level of protein and mRNA was demonstrated at the beginning of the disease. EMSAs showed: a.) a binding of HIF-1 to HRE and/or CRE, b.) in the same time the binding of CREB- I was detected to both HRE and/or CRE sequences in the liver, kidney and lung of diabetic animals. Based on these in vivo observations it is supposed that HRE and CRE through the interaction between HIF-1 and CREB-1 are equally involved in the regulation of VEGF expression at the onset of diabetes.
journal_name
Life Scijournal_title
Life sciencesauthors
Braun L,Kardon T,Reisz-Porszasz ZS,Banhegyi G,Mandl Jdoi
10.1016/s0024-3205(01)01327-3subject
Has Abstractpub_date
2001-10-12 00:00:00pages
2533-42issue
21eissn
0024-3205issn
1879-0631pii
S0024-3205(01)01327-3journal_volume
69pub_type
杂志文章相关文献
LIFE SCIENCES文献大全abstract::Mutations that increase constitutive activity and alter ligand binding have been used to investigate the structure and mechanism of activation of muscarinic receptors. These data are reviewed with reference to the recently published three-dimensional structure of rhodopsin. Residues in TM3 and TM6 where amino acid sub...
journal_title:Life sciences
pub_type: 杂志文章,评审
doi:10.1016/s0024-3205(01)01046-3
更新日期:2001-04-27 00:00:00
abstract::Using a two-lever drug discrimination procedure, six rats were trained to discriminate 0.5 mg/kg of racemic 1-(2,5-dimethoxy-4-iodophenyl)-2-aminopropane (DOI) from saline. Once trained, the animals demonstrated a dose-related decrease in discriminative performance upon administration of lower doses of DOI (ED50 = 0.1...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/0024-3205(86)90461-3
更新日期:1986-09-01 00:00:00
abstract::Alpha2-adrenoceptor (alpha2-AR)-regulated binding of the labelled GTP analog, guanosine 5'-O-(3-[35S]thiotriphosphate) ([35S]GTPgammaS), to guanine-nucleotide-binding proteins (G proteins) was studied in human platelet membranes. Under optimal conditions, the potent alpha2-AR agonist, 5-bromo-6-(2-imidazolin-2-ylamino...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/s0024-3205(99)00074-0
更新日期:1999-01-01 00:00:00
abstract::The human placental villus tissue contains opioid receptors and peptides. The opioid peptides extracted from the villus tissue were fractionated using reverse-phase high performance liquid chromatography and a radio-receptor assay. The presence of dynorphin 1-8 was corroborated by mass spectrometric production of (M +...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/0024-3205(87)90099-3
更新日期:1987-05-25 00:00:00
abstract::There is now some evidence that i) the availability of plasma tryptophan, the precursor of serotonin, is significantly lower in pregnant women at the end of term and the first few days after delivery than in nonpregnant women; and ii) both pregnancy and the early puerperium are accompanied by activation of the inflamm...
journal_title:Life sciences
pub_type: 临床试验,杂志文章
doi:10.1016/s0024-3205(02)01853-2
更新日期:2002-09-06 00:00:00
abstract::The regulation of the production of platelet-derived growth factor (PDGF) and the influence of high glucose concentration, eicosapentaenoic acid (EPA) were studied in cultured human umbilical vein endothelial cells (HUE). The PDGF production of HUE increased markedly depending on glucose concentration. However, EPA (3...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/0024-3205(95)00261-4
更新日期:1995-01-01 00:00:00
abstract::Several neuronal cell lines were screened for endogenous expression of neuronal nitric oxide synthase (nNOS) protein using Western blot analysis. Detectable levels of the nNOS protein were evident in the SK-N-SH, SH-SY5Y, and N1E-115 neuroblastoma cell lines, as well as the NG108-15 neuroblastoma x glioma hybrid. Only...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/s0024-3205(02)01732-0
更新日期:2002-07-05 00:00:00
abstract::Intrathecal (i.t.) injection (between lumbar vertebrae 5 and 6) into mice of a markedly low dose of IL-1alpha (3x10(-4) fmol or 5.4 fg in 5 microl per mouse) induced behaviors involving scratching, biting, and licking of non-stimulated hindpaws. The IL-1-induced behaviors appeared within 10 min of the injection of IL-...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/s0024-3205(99)00244-1
更新日期:1999-01-01 00:00:00
abstract::The central interference induced by dexamethasone and RU-38486, (a glucocorticoid receptor antagonist) on morphine antinociception were studied by using the tail flick test in mice. Dexamethasone, injected intracerebrally (i.c.v.) 10 minutes before morphine, dose-dependently reduced morphine antinociception, whereas R...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/0024-3205(92)90521-p
更新日期:1992-01-01 00:00:00
abstract:AIMS:Previous investigations by our group have shown that prenatal treatment with lipopolysaccharide (LPS; 100 μg/kg, intraperitoneally) on gestation day (GD) 9.5 in rats, which mimics infections by Gram-negative bacteria, induces short- and long-term behavioral and neuroimmune changes in the offspring. Because LPS ind...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/j.lfs.2014.10.019
更新日期:2015-01-01 00:00:00
abstract::To study the effects of volume overload and renal failure on plasma levels of immunoreactive atrial natriuretic hormone (IR-ANH), we measured levels of this hormone in normal subjects, in patients with advanced chronic renal failure (CRF) with and without clinically evident volume overload, and in patients with end-st...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/0024-3205(87)90732-6
更新日期:1987-09-28 00:00:00
abstract::Our previous research with long term cannabis users established an impaired ability to focus attention and filter out irrelevant information, which was progressive with the cumulative duration of exposure to cannabis. The current study examined these processes in a group of ex-cannabis users. The results suggested a p...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/0024-3205(95)00197-e
更新日期:1995-01-01 00:00:00
abstract::The effects of unilateral intracarotid administration of MPTP (1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine) in sheep were studied with the goal of producing a non-primate, large animal model of Parkinson's Disease. Adult female sheep were given an acute (over 30 min) or chronic (over 1 week) injection of MPTP (0.4-5....
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/0024-3205(94)00406-4
更新日期:1994-01-01 00:00:00
abstract:AIMS:Evidence has indicated U50,488H, a selective κ-opioid receptor (κ-OR) agonist, administered before ischemia attenuates apoptosis and infarction during ischemia and reperfusion (I/R). However, it remains unclear whether U50,488H postconditioning reduces apoptosis during I/R. This study was designed, therefore, to t...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/j.lfs.2010.10.018
更新日期:2011-01-03 00:00:00
abstract::Bile duct ligation (BDL) in rats induces portal fibrosis. This process has been linked to changes in the oxidative state of the hepatic cells and in the production of nitric oxide. Our objective was to find possible temporal connections between hepatic redox state, NO synthesis and liver injury. In this work we have c...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/j.lfs.2004.01.017
更新日期:2004-06-25 00:00:00
abstract::NMDA receptors are glutamate-regulated ion channels of critical importance for many neurophysiological and neuropathological processes. Mg2+ blocks the NMDA receptor by binding to the channel pore with an apparent affinity that depends on the membrane potential. We have investigated the effect of NMDA and the required...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/s0024-3205(01)00975-4
更新日期:2001-03-09 00:00:00
abstract:BACKGROUND:Cardiovascular disease (CVD) is the leading cause of mortality worldwide. Cardiac fibrosis is the scarring process occurs commonly with CVDs impairing the function and structure of heart. Herein, we investigated the role of circPAN3 in the pathogenesis of cardiac fibrosis. METHODS:A rat myocardial infarctio...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/j.lfs.2020.118015
更新日期:2020-09-15 00:00:00
abstract::Reoxygenation of the ischemic tissue promotes the generation of various reactive oxygen metabolites (ROM) which are known to have deleterious effects on various cellular functions. This study was designed to determine the possible protective effect of mesna (2-Mercaptoethane Sulfonate) on renal ischemia/reperfusion (I...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/j.lfs.2004.04.029
更新日期:2004-09-24 00:00:00
abstract:OBJECTIVE:To investigate the expression and time-dependent manner of bradykinin (BK) as well as its receptors (Bradykinin receptors 1 and 2, B1R and B2R) in spinal cord ischemia-reperfusion injury (SCII) in rat model. METHODS:Sprague-Dawley (SD) rats were subjected to 1 h of infra-renal abdominal aorta occlusion and r...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/j.lfs.2018.12.034
更新日期:2019-02-01 00:00:00
abstract:AIMS:Ischemia-reperfusion (I/R) event in vascular and nervous system has been documented to rising ischemic and vasculitic neuropathic pain, clinically resembles the complex regional pain syndrome (CRPS). The present study evaluated the effect of montelukast, a cysteinyl leukotriene receptor (Cys-LTC(4) and Cys-LTD(4))...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/j.lfs.2012.03.010
更新日期:2012-05-22 00:00:00
abstract::Our previous biochemical and mechanical studies have demonstrated an increase in Ca2+ sensitivity of cardiac myofilaments in ovariectomized rats. To test whether the body weight gain associated with ovariectomy contributed some effects to the changes in myofibrillar functions, the relations of pCa (-log Ca2+ molar con...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/s0024-3205(99)00623-2
更新日期:2000-01-01 00:00:00
abstract::The cytokeratins from human bladder and esophageal epithelia were separated using chromatographic techniques. The cytokeratins were first extracted from fresh autopsy tissue using high and low salt buffers. Urea, 8.0-9.5 M, was used to solubilize the resulting cytokeratin pellet. Imidazole was found to increase the so...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/0024-3205(90)90576-d
更新日期:1990-01-01 00:00:00
abstract::To evaluate the relative contributions of the ovarian inhibin and estradiol-17 beta (E) on the regulation of FSH secretion, inhibin and E in ovarian venous plasma (OVP) and FSH and LH in peripheral plasma were simultaneously measured using superovulating rats with special reference to follicular maturation. By the tra...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/0024-3205(86)90445-5
更新日期:1986-07-14 00:00:00
abstract::Amyloid beta-protein (A beta), the major protein of cerebrovascular and plaque amyloid in Alzheimer disease, is considered a primary factor in the pathology of this disease. The effect of synthetic A beta (1-40) on the activity of protein kinase C (PKC) was studied with histones for a substrate in a mixed micellar ass...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/0024-3205(91)90328-9
更新日期:1991-01-01 00:00:00
abstract::To analyze the metabolisms of platelet-activating factor (PAF) and Thromboxane A2 (TxA2) when platelets and mesangial cells (MC) interact, immunoreactive thromboxane B2 (TxB2) and PAF were measured after incubation of cultured rat MC with platelets (P) and with platelet supernatants (PS). In both cases, TxB2 significa...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/0024-3205(95)02030-m
更新日期:1995-01-01 00:00:00
abstract::It is well established that reperfusion of heart is the optimal method for salvaging ischemic myocardium, however, the success of this therapy could be limited by reperfusion injury, which is involved in inflammatory responses. High density lipoprotein (HDL) has an anti-inflammatory function and can protect the heart ...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/j.lfs.2007.06.021
更新日期:2007-08-09 00:00:00
abstract::A number of potential functions of thioredoxin have been proposed in literature, including a role for DNA replication. The aim of our study was to investigate the effects of thioredoxin from Streptomyces aureofaciens (Trx S.a.) on plasmid DNA. Trx S.a. was incubated with plasmid forms and the incubation product(s) cha...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/s0024-3205(97)01133-8
更新日期:1998-01-01 00:00:00
abstract:AIM:There is a specific frequency of extremely low-frequency electromagnetic field (ELF-EMF) that promotes neuronal differentiation. Although several mechanisms are known to regulate ELF-EMF-induced neuronal differentiation, a key factor that mediates neurogenic potentials by the ELF-EMF is largely unknown. Also, the p...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/j.lfs.2014.02.022
更新日期:2014-04-25 00:00:00
abstract::In our previous paper (Biochem. Biophys. Res. Commun. 141, 20-26 (1986) we reported the isolation of a 34000-dalton protein (p34K) which binds to calmodulin and F-actin from chicken gizzard smooth muscle. We examined the distribution of the immunoreactive component of gizzard p34K in bovine tissues by immunoblot analy...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/0024-3205(87)90151-2
更新日期:1987-07-20 00:00:00
abstract::Fenfluramine, a drug that induces increased synaptic serotonin, was used to train Fawn-Hooded rats in a drug discrimination paradigm. This strain of rats is thought to possess a genetic serotonin storage abnormality. The intent of the study was to see if the Fawn-Hooded rat was similar or dissimilar to the more freque...
journal_title:Life sciences
pub_type: 杂志文章
doi:
更新日期:1997-01-01 00:00:00