Abstract:
:The Ca2+ ionophore A23187 (0.2-5 microM) stimulates the phosphorylation of the substrates of protein kinase C (40,000 dalton protein) and myosin light chain kinase (20,000 dalton protein) in the presence or absence of cyclooxygenase inhibitors. In the presence of cyclooxygenase inhibitors or millimolar Ca2+ there is no stimulation of phospholipase C by A23187. Fingerprints of the 32P-labeled 40,000 dalton protein isolated from platelets that have been stimulated with A23187, thrombin, phorbol 12,13-dibutyrate and 1,2-didecanoylglycerol were identical. Higher concentrations of A23187 (1-5 microM) induced the loss of polyphosphoinositides through phosphomonoesterase activity.
journal_name
Life Scijournal_title
Life sciencesauthors
Lapetina EG,Reep B,Watson SPdoi
10.1016/0024-3205(86)90024-xsubject
Has Abstractpub_date
1986-08-25 00:00:00pages
751-9issue
8eissn
0024-3205issn
1879-0631pii
0024-3205(86)90024-Xjournal_volume
39pub_type
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