Abstract：
:Peptide Histidine Isoleucine (PHI) is generally considered a low affinity agonist for Vasoactive Intestinal Peptide (VIP) receptors. In this study, we investigated the presence and characteristics of [125I]PHI binding sites on rat liver membranes. Detergents at nonsolubilizing concentrations (1 mM CHAPS or 0.01% Tween-20) were included in the assay buffer to reduce adsorptive loss of PHI to acceptable levels and permit measurement of PHI-binding to receptors. Under these conditions, binding of PHI to liver membranes was time- and temperature-dependent, reversible and saturable. Unlabeled PHI was 9.7-fold more potent than VIP, and 357-fold more potent than secretin in displacing [125I]-PHI binding. Scatchard analysis suggested the presence of two classes of PHI receptors, with Kd 27 pM and 512 pM. The data from [125I]-PHI and [125I]-VIP binding studies suggested that one class of receptors was PHI-preferring, and the other, equally reactive with PHI and VIP. The concentration of immunoreactive PHI, measured by radioimmunoassay, in blood from the hepatic portal vein of anesthetized rats was 2-fold higher than that from the hepatic vein, suggesting uptake of circulating PHI by the liver.
journal_name
Life Scijournal_title
Life sciencesauthors
Paul S,Chou J,Kubota Edoi
10.1016/0024-3205(87)90661-8subject
Has Abstractpub_date
1987-11-23 00:00:00pages
2373-80issue
21eissn
0024-3205issn
1879-0631pii
0024-3205(87)90661-8journal_volume
41pub_type
杂志文章相关文献
LIFE SCIENCES文献大全abstract：:Effects of amphetamine on concentrations of dopamine, norepinephrine and serotonin in several monoamine-containing cell body and terminal regions were examined left and right separately in rats. Results suggest that amphetamine reduced the L-R asymmetry of most of these measures, and this effect is more significant in...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/0024-3205(87)90334-1
更新日期：1987-04-06 00:00:00
abstract：AIMS:Oxidative stress emerges after reperfusion of an organ following an ischemic period and results in tissue damage. In the heart, an amplified generation of reactive oxygen species and a significant Ca(2+) accumulation cause ventricular arrhythmias and mitochondrial dysfunction. This occurs in consequence of increas...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/j.lfs.2013.12.026
更新日期：2014-02-06 00:00:00
abstract：:Although lipid peroxidation of biological samples may be assessed by different chemical and physical methods, those based on the measurement of malondialdehyde formed from the breakdown of endoperoxides during the last stages of the oxidation of a polyunsaturated fatty acid, appear as the most widely used. Among the v...
journal_title：Life sciences
pub_type： 杂志文章,评审
doi：10.1016/0024-3205(91)90550-u
更新日期：1991-01-01 00:00:00
abstract：:Proteinase inhibitors were tested for their ability to inhibit prolactin (PRL) and growth hormone (GH) release by cultured anterior pituitary cells of the rat. Inhibitors of microbial origin (chymostatin, elastatinal, leupeptin) had either no or a moderate effect on hormone release while some tripeptide aldehydes, esp...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/0024-3205(85)90636-8
更新日期：1985-02-11 00:00:00
abstract：:This study examined the effect of nitric oxide (NO) on the cytosolic free Ca(2+) concentration ([Ca(2+)](c)) of alpha-cells isolated from rat pancreatic islets. When extracellular glucose was reduced from 7 to 0 mM, about half of the alpha-cells displayed [Ca(2+)](c) oscillations. Nicardipine, a Ca(2+) channel blocker...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/s0024-3205(02)01608-9
更新日期：2002-05-24 00:00:00
abstract：:The present study attempts to indirectly determine if a neuronal cannabinoid (CB1) receptor mediates the intraocular pressure (IOP) reduction effects of arachidonoyl ethanolamide (AEA), its R-alpha-isopropyl analog, and the non-classical cannabinoid, CP-55,940. A series of these cannabinoids were dissolved in an aqueo...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/s0024-3205(98)00499-8
更新日期：1998-01-01 00:00:00
abstract：:18-Hydroxycorticosterone (18-OHB) is a precursor of aldosterone and is the only corticosteroid, other than aldosterone, that is synthesized predominantly in the zona glomerulosa. Administration of the dopamine antagonist, metoclopramide results in parallel rises in plasma 18-OHB and aldosterone levels without affectin...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/0024-3205(83)90157-1
更新日期：1983-12-19 00:00:00
abstract：:It has been previously shown in anesthetized pigs that intravenous infusion of 2 microg/h of 17beta-estradiol primarily dilated renal, iliac and coronary circulations, while higher doses of the hormone were required to cause vasodilation also in the mesenteric vascular bed. In the same experimental model, a tonic beta...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/s0024-3205(99)00399-9
更新日期：1999-01-01 00:00:00
abstract：:We have investigated the possible involvement of arachidonic acid metabolites in dopamine-induced inhibition of adrenocortical steroidogenesis. Administration of dopamine (5 x 10(-5) M) for 20 min to perifused frog adrenal slices caused a marked reduction of the release of both prostaglandin E2 (PGE2) and 6-keto-PGF1 ...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/0024-3205(89)90292-0
更新日期：1989-01-01 00:00:00
abstract：:Liver fibrosis is a global health issue that causes morbidity and mortality with no currently available treatment. It has been shown that low dose paclitaxel (PTX) can stabilize microtubules and inhibit the profibrotic transforming growth factor-beta 1 (TGF-β1) signaling pathway. In this study the effect of treatment ...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/j.lfs.2018.09.037
更新日期：2018-10-15 00:00:00
abstract：:The effect of resveratrol, a polyphenolic compound present in grapes and other plants, on proteinuria, hypoalbuminemia and hyperlipidemia was studied in rats with glomerulonephritis. The nephritis was induced by an intravenous injection of anti-rat kidney glomerular basement membrane rabbit antiserum. Nephritic rats w...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/s0024-3205(01)01061-x
更新日期：2001-05-11 00:00:00
abstract：AIM:The purpose of this study was to investigate whether the flavonoid quercetin can prevent alterations in the behavioral tests and of cholinergic neurotransmission in rats submitted to the ethidium bromide (EB) experimental demyelination model during events of demyelination and remyelination. MAIN METHODS:Wistar rat...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/j.lfs.2014.03.033
更新日期：2014-05-17 00:00:00
abstract：:This paper describes the use of complex liposomes as real membrane models to evaluate the potential benefits of several antioxidants in relation to lipid peroxidation. The xanthine oxidase/Fe(3+)-ADP-EDTA and the Fe(2+)/H2O2 systems have been used to generate hydroxyl radicals and the water soluble azo-compound 2,2'-a...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/s0024-3205(03)00120-6
更新日期：2003-04-11 00:00:00
abstract：:Linoleic acid (LA) (n-6) and α-linolenic acid (ALA) (n-3) are essential fatty acids (EFAs) as they cannot be synthesized by humans or other higher animals. In the human body, these fatty acids (FAs) give rise to arachidonic acid (ARA, n-6), eicosapentaenoic acid (EPA, n-3), and docosahexaenoic acid (DHA, n-3) that pla...
journal_title：Life sciences
pub_type： 杂志文章,评审
doi：10.1016/j.lfs.2018.04.049
更新日期：2018-06-15 00:00:00
abstract：:The present study was designed to investigate the effects of chronic administration of the alcoholic extract of Terminalia arjuna (TAAE) bark on isoproterenol induced myocardial injury. The TAAE was administered orally to Wistar albino rats (150-200 g) in three different doses, by gastric gavage [3.4 mg/kg: (T1), 6.75...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/s0024-3205(03)00671-4
更新日期：2003-10-10 00:00:00
abstract：:Glucocorticoid receptors (GR) are ubiquitous molecules and are present also in the hippocampus and in several other nervous and immune tissues. Peripheral blood mononuclear cells (PBMCs) are a good model for studies of GR in humans. Glucocorticoids are important for maintaining cellular and humoral homeostasis and are...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/s0024-3205(97)00386-x
更新日期：1997-01-01 00:00:00
abstract：:This study was performed in order to investigate the type of T cells recruited to the blood in response to an acute bout of exercise with regard to mean lengths of telomeric terminal restriction fragments (TRF) and surface activation markers and with special emphasis on age-associated differences. Ten elderly and ten ...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/s0024-3205(99)00531-7
更新日期：1999-01-01 00:00:00
abstract：AIMS:The aims of this study were to examine the role of endothelin-1 (ET-1), a pleiotropic peptide found at elevated levels in a number of malignancies and which has been shown to influence oral cancer cell behaviour via paracrine signalling pathways, on the phenotype of oral fibroblasts. MAIN METHODS:The effect of ET...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/j.lfs.2012.04.001
更新日期：2012-10-15 00:00:00
abstract：:Thyrotropin-releasing hormone (TRH) produces a marked pressor effect, which may be mediated by central cholinergic neurons, which in turn enhance sympathetic nervous system activity. In this study, 22 subjects (10 patients with Alzheimer's disease and 12 elderly controls) were administered IV scopolamine or placebo pr...
journal_title：Life sciences
pub_type： 临床试验,杂志文章,随机对照试验
doi：10.1016/0024-3205(94)00629-6
更新日期：1994-01-01 00:00:00
abstract：:The pupillary effects of intravenous buprenorphine were studied in eight nondependent male subjects who reported previous opiate use. Buprenorphine (0.3, 0.6, and 1.2 mg) decreased pupil size, the amplitude of the light reflex, and the velocities of constriction and dilation. Significant pupillary effects occurred wit...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/0024-3205(91)90026-8
更新日期：1991-01-01 00:00:00
abstract：:Metalloproteinases (MMP), particularly MMP-9 produced by the intratumor monocyte/macrophages, play an important role in tumor invasion and metastases. Recent clinical trials in patients with primary breast cancer suggest that bisphosphonates (BP), above all clodronate, may reduce bone metastases. The aim of the presen...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/s0024-3205(03)00657-x
更新日期：2003-09-26 00:00:00
abstract：AIMS:In myocardial ischemia-reperfusion (MI/R) injury, impaired autophagy function worsens cardiomyocyte death. AMP-activated protein kinase (AMPK) is a heterotrimeric protein that plays an important role in cardioprotection and myocardial autophagic function. AMPKα1 and α2 are localized primarily in the cytoplasm and ...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/j.lfs.2019.04.002
更新日期：2019-05-15 00:00:00
abstract：:Recent reports indicate that some imidazole-5-acetic acid derivatives are competitive antagonists of angiotensin II receptors. However, to our knowledge, there is no published information regarding: 1) what constant infusion rate of these non-peptide angiotensin receptor blockers is necessary to effectively antagonize...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/0024-3205(90)90096-a
更新日期：1990-01-01 00:00:00
abstract：:Down syndrome (DS) is the most common chromosomal abnormality and is associated with an extra copy of the chromosome 21. Although several markers are commonly used during pregnancy for the screening of DS, the definitive diagnosis is based on karyotype after amniocentesis, which is an expensive and laborious analysis....
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/j.lfs.2004.09.003
更新日期：2004-12-10 00:00:00
abstract：:We have evaluated the effect of intraperitoneal (I.P.) injection of human recombinant interferon-2alpha (IFN-alpha) on Bleomycin-induced pulmonary injury in hamsters. Pulmonary injury was induced by a single intratracheal (I.T.) instillation of Bleomycin (Bleo). Six groups of male Syrian hamsters were treated as follo...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/s0024-3205(97)00332-9
更新日期：1997-01-01 00:00:00
abstract：:Intracerebroventricular (i.c.v.) administration of thyrotropin-releasing hormone (TRH) in a range from 0.1 to 100 micrograms induced a dose-related increase in blood pressure in conscious rats, whereas TRH-free acid (TRH-OH) and histidyl-proline diketopiperazine (His-Pro-DKP), metabolites of TRH, did not. The blood pr...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/0024-3205(87)90586-8
更新日期：1987-03-30 00:00:00
abstract：:In this study we compared the antioxidant properties of five different extracts of different composition obtained from Achyrocline satureioides' inflorescences (Compositae), a widely used Brazilian folk medicinal herb. All of the extracts presented significant antioxidant potential identified by TRAP assay, which incr...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/j.lfs.2003.09.073
更新日期：2004-04-23 00:00:00
abstract：AIMS:The clinical efficiency of dendritic cell (DC) therapy needs to be improved. Exosomes, as membrane nano-vesicles, carry bio-macromolecules and play essential roles in intercellular crosstalk. Here, it is proposed that tumor cell-derived exosomes could function as vehicles to deliver exogenous miRNA-155 into DCs, f...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/j.lfs.2019.01.005
更新日期：2019-02-15 00:00:00
abstract：:Using liposomes differing in size and lipid composition, we have studied the uptake characteristics of the liver parenchymal and Kupffer cells. Desferal labeled with iron-59 was chosen as a radiomarker for the liposomal content, because Desferal in its free form does not cross cellular membranes. At various time inter...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/0024-3205(82)90098-4
更新日期：1982-11-08 00:00:00
abstract：:Previous studies have shown that cytochrome P450-Arachidonic Acid (P450-AA) metabolites modify the vascular tone of several vessels and that vasopressin (AVP) stimulates P450-AA metabolism. Thus, in the present study, we decided to investigate if the vasoconstrictor effect of AVP is related to activation of P450-AA me...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/0024-3205(95)00108-i
更新日期：1995-01-01 00:00:00
