Release of (14C)5-hydroxytryptamine from human platelets by red wine.

abstract：:Experiments were done to examine the analgesic effect of thiorphan alone or in combination with stress in mice. Analgesia was assessed by measuring jump latencies from a 55 degrees C hot plate. Thiorphan exhibited weak analgesic properties evidenced by significant increases in jump latencies only after 300 mg/kg i.p. ...

journal_title：Life sciences

authors： Greenberg R,O'Keefe EH

更新日期：1982-09-20 00:00:00

abstract：:The loss of osteoblast function in aging bone is one of the major causes of osteopenia, or loss of bone mass. In this study, this loss of function was investigated by examining the proliferative response of rat long bone periosteal osteoblasts to TGF(beta1) and 1,25-dihydroxy vitamin D(3) (1,25-D(3)) as a function of ...

journal_title：Life sciences

authors： Shiels MJ,Mastro AM,Gay CV

更新日期：2002-05-10 00:00:00

abstract：:We have recently shown that the vasoconstrictor serotonin (5-HT) inhibits oxygen uptake in perfused hindlimb possibly due to vascular shunting. Thus in the present study the effect of 5-HT on insulin-mediated glucose uptake was assessed. Rat hindlimbs were perfused at constant flow with medium containing 8.3 mM glucos...

journal_title：Life sciences

authors： Rattigan S,Dora KA,Colquhoun EQ,Clark MG

更新日期：1993-01-01 00:00:00

abstract：:Previously we have reported a decreased GABAB receptor expression in brain of spontaneously hepertensive rats (SHR). Moreover, decreased receptor numbers were accompanied by depressed stimulation of cAMP formation. We have now quantified Gi alpha protein by immunoblotting in cerebral membranes from 11-week-old normote...

journal_title：Life sciences

authors： Ichida T,Takeda K,Sasaki S,Nakagawa M,Kuriyama K

更新日期：1996-01-01 00:00:00

abstract：AIMS:Normal human cells in culture progressively lose their capacity for replication, ending in an irreversible arrested state known as replicative senescence. Senescence has been functionally associated to the process of organismal ageing and is also considered a major tumor-suppressing mechanism. Although a great dea...

journal_title：Life sciences

authors： Maeda M,Scaglia N,Igal RA

更新日期：2009-01-16 00:00:00

abstract：:The role of islet amyloid polypeptide, also known as amylin, in insulin resistance and in the etiology of diabetes has been a subject of debate. Increased plasma amylin levels have been observed in both obese and type II diabetic patients. However, data on endogenous amylin levels with relation to pharmacological inte...

journal_title：Life sciences

authors： Gill AM,Yen TT

更新日期：1991-01-01 00:00:00

abstract：:The effect of the Type I benzodiazepine (BDZ) receptor agonist, CL 218,872, on convulsions generated by low doses of methyl beta-carboline-3-carboxylic acid ( MBCC ), bicuculline, picrotoxin and pentylenetetrazole (PTZ) in mice was examined. Low doses of CL 218,872 enhanced the convulsions produced by all agents excep...

journal_title：Life sciences

authors： Melchior CL,Garrett KM,Tabakoff B

更新日期：1984-05-28 00:00:00

abstract：:Recent reports indicate that some imidazole-5-acetic acid derivatives are competitive antagonists of angiotensin II receptors. However, to our knowledge, there is no published information regarding: 1) what constant infusion rate of these non-peptide angiotensin receptor blockers is necessary to effectively antagonize...

journal_title：Life sciences

authors： Jackson EK,Inagami T

更新日期：1990-01-01 00:00:00

abstract：:Meso-dimercaptosuccinic acid (DMSA) and the sodium salt of 2,3-dimercapto-1-propanesulfonic acid (DMPS) are analogous in chemical structure to dimercaprol (BAL, British Anti-Lewisite). Dimercaprol was among the first therapeutically useful metal chelating agents and was developed originally as an anti-lewisite agent. ...

journal_title：Life sciences

authors： Aposhian HV,Mershon MM,Brinkley FB,Hsu CA,Hackley BE

更新日期：1982-11-08 00:00:00

abstract：:In this study we analyzed the N-formyl-Met-Leu-Phe (fMLP)-induced calcium signal in alveolar macrophages (AM) isolated from ovalbumin-sensitized (OA-sensitized AM) and naive (naive AM) guinea-pigs. Guinea-pigs were sensitized by subcutaneous injection of OA and AM were isolated by bronchoalveolar lavage 6 weeks therea...

journal_title：Life sciences

authors： Failli P,Bindi D,Franceschelli F,Tanini A,Ciuffi M,Mazzetti L,Zilletti L

更新日期：2001-08-24 00:00:00

abstract：:Hypercalcemic strains of the rat Walker 256 (W256) tumor synthesize parathyroid hormone-related protein (PTHrP) and at least one of them produces an ill-defined transforming growth factor activity. We tested the production of transforming growth factor (TGF) beta by a hypercalcemic W256 tumor strain, and assessed its ...

journal_title：Life sciences

authors： Benítez-Verguizas J,Loarte D,de Miguel F,Esbrit P

更新日期：1999-01-01 00:00:00

abstract：:Mucosal mast cells are implicated in visceral hypersensitivity associated with irritable bowel syndrome (IBS). In this study, we investigated the role of mast cells in the development of visceral hypersensitivity by using mast cell deficient (Ws/Ws) rats and their control (W+/W+). In W+/W+ rats, an injection of 2,4,6-...

journal_title：Life sciences

authors： Ohashi K,Sato Y,Kawai M,Kurebayashi Y

更新日期：2008-02-13 00:00:00

abstract：:Recent findings indicate that low concentrations of dopamine (DA) stimulate the secretion of prolactin (PRL) in vitro. In this study, we found that low concentrations of the highly-specific DA D2 receptor agonist, quinpirole hydrochloride (LY) stimulate PRL secretion in female rats, assessed by reverse hemolytic plaqu...

journal_title：Life sciences

authors： Niimi M,Takahara J,Sato M,Murao K,Kawanishi K

更新日期：1993-01-01 00:00:00

abstract：:The level of hepatic lipidperoxides in chick embryos was determined during cataract formation resulting from glucocorticoid treatment. When 15 day old chick embryos were administered 0.25 mumol of hydrocortisone acetate their hepatic lipidperoxide level, determined by thio-barbituric acid, increased after a lag time o...

journal_title：Life sciences

authors： Nishigori H,Lee JW,Iwamoto Y,Hayashi R,Maruyama K,Iwatsuru M

更新日期：1984-08-27 00:00:00

abstract：:It has been previously shown in anesthetized pigs that intravenous infusion of 2 microg/h of 17beta-estradiol primarily dilated renal, iliac and coronary circulations, while higher doses of the hormone were required to cause vasodilation also in the mesenteric vascular bed. In the same experimental model, a tonic beta...

journal_title：Life sciences

authors： Molinari C,Battaglia A,Grossini E,Mary DA,Surico N,Vacca G

更新日期：1999-01-01 00:00:00

abstract：:The binding of different gangliosides to rat T-helper lymphocytes was characterized under conditions that decrease CD4 expression on different mammalian T-helper lymphocytes. Saturation binding by monosialylated [3H]-GM1 to rat T-lymphocytes was time- and temperature-dependent, had a dissociation constant (KD) of 2.2 ...

journal_title：Life sciences

authors： Morrison WJ,Offner H,Vandenbark AA

更新日期：1989-01-01 00:00:00

abstract：:We find a significantly lower level of specific [3H]nicotine binding in the fetal rat forebrain of 20 day gestational age as compared to adult rat tissue, and a progressive increase in the [3H]nicotine binding in the early neonatal forebrain, reaching adult level at 14-28 days of age. The maximal binding sites (Bmax) ...

journal_title：Life sciences

authors： Yamada S,Kagawa Y,Isogai M,Takayanagi N,Hayashi E

更新日期：1986-02-17 00:00:00

abstract：:Nitric oxide (NO) is generated from L-arginine by different isoforms of the enzyme nitric oxide synthase (NOS) and is known to be involved in mediating several biological functions, some of which are associated with reproduction. In this study, we examined the ability of the prepubertal ovary to express the inducible ...

journal_title：Life sciences

authors： Srivastava V,Jones BJ,Dookwah H,Hiney JK,Dees WL

更新日期：1997-01-01 00:00:00

abstract：AIMS:Various investigations have demonstrated the protective capacity of general anesthetics as neuroprotective agents. The effects of propofol against ischemia are known to reside in its antioxidant properties and its GABAergic activity. Other aromatic alcohols have also been reported as able to protect neurons agains...

journal_title：Life sciences

authors： Delgado-Marín L,Sánchez-Borzone M,García DA

更新日期：2017-04-15 00:00:00

abstract：:We investigated whether calcium channel antagonists would alter the induction of nitric oxide (NO) synthesis by bacterial lipopolysaccharide (LPS) alone or in combination with interferon-gamma (IFN gamma) in cultured J774 macrophages, rat vascular smooth muscle cells, rat renal mesangial cells, and rat cardiac myocyte...

journal_title：Life sciences

authors： Hattori Y,Kasai K,So S,Hattori S,Banba N,Shimoda S

更新日期：1995-01-01 00:00:00

abstract：:The role of Na+ and Ca2+ channels in glutamate-mediated hypoxic injury was investigated in slices of the rat cerebral cortex. Hypoxic injury was determined by mitochondrial reduction of 3-(4,5-dimethyl-2-thiazol)-2,5-diphenyltetrazolium bromide after exposure of brain slices to 30-min of hypoxia/glucose deprivation fo...

journal_title：Life sciences

authors： Oka M,Itoh Y,Ukai Y

更新日期：2000-09-29 00:00:00

abstract：:From the fruiting bodies of the edible mushroom Flammulina velutipes a single-chained ribosome inactivating protein with a molecular weight of 13.8 kDa was isolated with a procedure involving ion exchange chromatography on DEAE-cellulose and SP-Sepharose and affinity chromatography on Affi-gel blue gel. The protein wa...

journal_title：Life sciences

authors： Wang H,Ng TB

更新日期：2001-03-23 00:00:00

abstract：:Angiotensin (AII) and atrial natriuretic peptide (ANP) exert opposite effects on phosphorylation of a 17.6 kDa nuclear protein from bovine adrenal glomerulosa cells. The protein was separated by sodium dodecyl sulfate electrophoresis and blotted onto polyvinylidene difluoride, and the N-terminal sequence was obtained....

journal_title：Life sciences

authors： Elliott ME

更新日期：1990-01-01 00:00:00

abstract：:Muscarinic receptors are expressed in smooth muscle throughout the body. In most instances, the muscarinic receptor population in smooth muscle is composed of mainly the M2 and M3 subtypes in an 80% to 20% mixture. The M3 subtype mediates phosphoinositide hydrolysis and calcium mobilization, whereas the M2 subtype med...

journal_title：Life sciences

authors： Ehlert FJ,Ostrom RS,Sawyer GW

更新日期：1997-01-01 00:00:00

abstract：:The binding of 3H-ouabain to human erythrocytes was analyzed in a population of hospitalized male ethanol addicted patients under long term digitalis treatment. In the non-alcoholic patient group the long term digitalis treatment induced an increase in Bmax and Kd values; such modification was not observed in the alco...

journal_title：Life sciences

authors： Battaini F,Govoni S,Mauri A,Civelli L,Trabucchi M

更新日期：1987-06-29 00:00:00

abstract：:Binding studies conducted on membrane preparation from human platelets using (3H) Ro5-4864 and (3H) diazepam showed specific and saturable binding. Scatchard analysis revealed a single class of binding sites with KD = 10.8 +/- 0.9 nM and Bmax = 775 +/- 105 fmol/mg protein for (3H) Ro5-4864 and KD = 10.5 +/- 1.1 nM and...

journal_title：Life sciences

authors： Moingeon P,Dessaux JJ,Fellous R,Alberici GF,Bidart JM,Motté P,Bohuon C

更新日期：1984-11-12 00:00:00

abstract：:To elucidate the pharmacological profile of P2X receptors and the probable expression of P2Y receptors in otic ganglion neurons from 17-day-old rats, single neurons were enzymatically isolated and maintained in tissue culture for up to 30 h. Whole-cell voltage-clamp recording was carried out at a holding potential of ...

journal_title：Life sciences

authors： Ma B,Yu LH,Fan J,Ni X,Burnstock G

更新日期：2008-08-01 00:00:00

abstract：:This paper describes the use of complex liposomes as real membrane models to evaluate the potential benefits of several antioxidants in relation to lipid peroxidation. The xanthine oxidase/Fe(3+)-ADP-EDTA and the Fe(2+)/H2O2 systems have been used to generate hydroxyl radicals and the water soluble azo-compound 2,2'-a...

journal_title：Life sciences

authors： Gutiérrez ME,García AF,Africa de Madariaga M,Sagrista ML,Casadó FJ,Mora M

更新日期：2003-04-11 00:00:00

abstract：:Phosphatidylinositol (PI) specific phospholipase C treatment of rabbit platelets caused 95% release of acetylcholinesterase in the supernatant and 4 to 6% hydrolysis of membrane PI in 2 min. Under these conditions there was no cell lysis as monitored by lack of lactate dehydrogenase activity in the medium. The phospho...

journal_title：Life sciences

authors： Shukla SD

更新日期：1986-02-24 00:00:00

abstract：AIMS:High level of saturated fatty acids leads to mitochondrial dysfunction and inflammation in the development of insulin resistance in skeletal muscle. We recently found that puerarin improved impaired insulin signaling in skeletal muscle in diabetic animals and in myotubes in vitro. However, whether puerarin can act...

journal_title：Life sciences

authors： Chen X,Yi L,Song S,Wang L,Liang Q,Wang Y,Wu Y,Gao Q

更新日期：2018-08-01 00:00:00