Abstract:
AIMS:High level of saturated fatty acids leads to mitochondrial dysfunction and inflammation in the development of insulin resistance in skeletal muscle. We recently found that puerarin improved impaired insulin signaling in skeletal muscle in diabetic animals and in myotubes in vitro. However, whether puerarin can act directly on muscle cells to alleviate lipid-induced mitochondrial dysfunction and inflammation remains obscure. This study was conducted to analyze the attributive properties of puerarin against mitochondrial dysfunction and inflammation in skeletal muscle cells with insulin resistance. MAIN METHODS:The effects of puerarin on mitochondrial biogenesis, oxidative phosphorylation, dynamics of fusion, fission and mitophagy, oxidative stress, as well as inflammatory response and insulin sensitivity in L6 myotubes treated with palmitate were examined. KEY FINDINGS:Puerarin pretreatment improve insulin sensitivity and prevented muscle cells from palmitate-induced mitochondrial dysfunction manifested by the increases of complex I activity, mitochondrial membrane potential and ATP generation, and the decrease of reactive oxygen species (ROS) production. Augmented expression of genes involved in mitochondrial biogenesis, oxidative phosphorylation, and the detoxification of ROS were also observed upon puerarin supplementation. Moreover, puerarin modulated mitochondrial fusion and fission, and rescued palmitate-impaired mitophagy via phosphatase and tensin homolog-induced putative kinase 1(PINK1)/Parkin pathway. In addition, puerarin attenuated palmitate-induced inflammation through the inhibition of toll-like receptor 4/nuclear factor-κB signaling pathway. SIGNIFICANCE:Our findings indicated that puerarin could act directly on muscle cells to attenuate palmitate-induced mitochondrial dysfunction, impaired mitophagy and inflammatory response, thereby contributing to the improvement of insulin sensitivity.
journal_name
Life Scijournal_title
Life sciencesauthors
Chen X,Yi L,Song S,Wang L,Liang Q,Wang Y,Wu Y,Gao Qdoi
10.1016/j.lfs.2018.05.041subject
Has Abstractpub_date
2018-08-01 00:00:00pages
84-92eissn
0024-3205issn
1879-0631pii
S0024-3205(18)30309-6journal_volume
206pub_type
杂志文章相关文献
LIFE SCIENCES文献大全abstract::A variety of chromatographic approaches are detailed for enhancement of signal and selectivity for determination of biogenic amines by HPLC with amperometric detection. Microbore HPLC columns offer significant signal enhancement due to the reduced elution volume. Selection of novel surfactants such as N-methyl oleoyl ...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/0024-3205(87)90189-5
更新日期:1987-08-17 00:00:00
abstract::The objective of this study was to investigate whether age induces changes on vasodilator response induced by cromakalim, an ATP-sensitive K+ (K(ATP)) channel opener, as well as the underlying mechanism involved in this possible alteration. For this purpose, aortic segments from young (3-5 months) and old (3 years) ra...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/s0024-3205(99)80003-4
更新日期:1998-01-01 00:00:00
abstract::The hypothermia induced by apomorphine, a mixed dopamine (DA) agonist in male Swiss-Webster mice, was not blocked by the selective D-1 antagonist SCH 23390 but was completely blocked by the selective D-2 antagonists haloperidol, sulpiride and YM-09151-2. The selective D-1 agonist SKF 38393 did not elicit hypothermic r...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/0024-3205(88)90515-2
更新日期:1988-01-01 00:00:00
abstract::When s-triazolo[3,4-a]phthalazine (Tri-P) was orally administered in rats, a more lipophilic metabolite M-1 than the parent compound was isolated from the urine. The metabolite M-1 was identified as 7-methylthio Tri-P by means of high resolution MS and two-dimensional NMR spectrometry. Furthermore, the 7-methylthio co...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/j.lfs.2003.06.032
更新日期:2003-11-21 00:00:00
abstract::Studies were performed in conscious, chronically catheterized male Sprague-Dawley rats to investigate the effect of administered atrial natriuretic peptide (ANP) on blood pressure, renal hemodynamics and urinary electrolyte excretion. Studies were performed on young adult (3-4 month old) rats and on aging rats (18-24 ...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/0024-3205(90)90066-z
更新日期:1990-01-01 00:00:00
abstract::We have developed and utilized a photoaffinity probe to identify the adenosine transporter in cardiac sarcolemmal (SL) vesicles. The probe is an azidosalicylate derivative of adenosine made by reacting adenosine with N-hydroxysuccinimidyl-4-azidosalicylic acid (NHS-ASA). Following synthesis and radiolabeling of the pr...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/0024-3205(95)00268-b
更新日期:1995-01-01 00:00:00
abstract::Preliminary data suggest that amygdalar corticotropin-releasing factor (CRF) is regulated by nicotinic agonists. We sought to confirm and extend these observations by determining the effects of various concentrations of nicotine on CRF messenger RNA expression in the AR-5 immortalized amygdalar cell line. Nicotine pro...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/s0024-3205(99)00539-1
更新日期:1999-01-01 00:00:00
abstract:AIMS:Systemic administration of opiate analgesics such as morphine remains the most effective treatment for alleviating severe pain across a range of conditions including acute pain. However, chronic or repeated administration of opiate analgesics results in the development of analgesic tolerance. Glial cells such as m...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/j.lfs.2013.08.009
更新日期:2013-10-11 00:00:00
abstract::There is increasing evidence that the 5'UTR of mRNAs affects regulation of gene expression in eukaryotic cells. We examined the overexpression of the mu-opioid receptor in High Five insect cells, employing rat mu-receptor cDNA linked to variable lenghts of their native 5'UTR. The sequences employed consist of either 2...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/s0024-3205(99)00017-x
更新日期:1999-01-01 00:00:00
abstract:AIM:This study was undertaken to determine the biological activity of a green tea supplement with respect to cells and erythrocyte membranes and the molecular mechanism of that activity. MAIN METHODS:The extract's activity was evaluated on the basis of its hemolytic, antioxidant and antiinflammatory actions. In additi...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/j.lfs.2014.12.025
更新日期:2015-04-01 00:00:00
abstract::Amyloid beta protein (Abeta) alters signal transduction systems, including increases in the cytosolic free calcium ([Ca2+]i) response which have pathophysiological significance in Alzheimer's disease (AD). The purposes of this study were to elucidate the mechanism involved in Abeta's effect on the Ca2+ signal and to e...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/s0024-3205(00)00470-7
更新日期:2000-01-01 00:00:00
abstract::Previous behavioral studies on triazolam (TZ), which are small in number, could only speculate about tolerance to the anxiolytic effect of TZ, as the experiments did not cover sufficient time (of 4 to 7 days) for tolerance to develop. Therefore longer time for chronic TZ administration is used. We investigated the eff...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/s0024-3205(03)00612-x
更新日期:2003-10-31 00:00:00
abstract::DL-alpha-difluoromethylornithine (DFMO), 4-deoxypyridoxine (4-DOP), and methylglyoxal bis (guanylhydrazone) (MGBG) were tested as inhibitors of acute skin graft rejection. Proximal full thickness tail skins were exchanged between C57BL/6 and Balb/C mice. Distal autografts were placed to monitor healing. Inhibitors wer...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/0024-3205(91)90349-g
更新日期:1991-01-01 00:00:00
abstract::In vitro studies showed that organophosphate insecticides have different IC50 values (i.e., concentration of inhibitor required to inhibit 50% enzyme activity) for acetylcholinesterases (AChE's) from different species. Since previous reports indicated that the binding of active cholinesterase inhibitors to non-critica...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/0024-3205(82)90426-x
更新日期:1982-07-12 00:00:00
abstract::The binding of membrane immunoglobulin (mIg) by anti-Ig antibodies is known to initiate a mitogenic signal in B lymphocytes. Because in many instances growth control appears to be correlated with phosphokinase activity, as well as with alterations in cytoskeletal architecture, we asked the question whether antibodies ...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/0024-3205(88)90089-6
更新日期:1988-01-01 00:00:00
abstract::This study examined the effects of chronic treatment of a new phosphodiesterase type 5 inhibitor, DA-8159, on endothelial dysfunction in stroke-prone spontaneously hypertensive rats (SHR-SP). Six-week-old male SHR-SP were divided into 4 groups; vehicle control, DA-8159 1, 3, and 10 mg/kg/day. During a 32-week experime...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/j.lfs.2005.06.042
更新日期:2006-02-09 00:00:00
abstract::Hepatotoxicity is a common side effect encountered with tamoxifen (TAM) administration. Due to the great value of TAM in breast cancer treatment, hepato-protection is seriously recommended. AIMS:The present study investigated the hepato-protective effect of celecoxib (CX) against TAM-induced hepatotoxicity in rats. ...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/j.lfs.2019.116573
更新日期:2019-08-15 00:00:00
abstract::The kinetic parameters of serotonin (5-HT) uptake and imipramine binding of the synaptosomes and blood platelets of male Fawn-hooded rats, which have a 5-HT storage abnormality, and normal Sprague-Dawley rats were compared. The Vmax for 5-HT uptake of synaptosomes from Fawn-hooded rats was significantly greater than t...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/0024-3205(83)90792-0
更新日期:1983-08-01 00:00:00
abstract::Cannabinoid agonists such as Delta9-tetrahydrocannabinol (THC) produce a wide range of pharmacological effects both in the central nervous system and in the periphery. One of the most striking features of cannabinoids such as THC is the magnitude to tolerance that can be produced upon repetitive administration of this...
journal_title:Life sciences
pub_type: 杂志文章,评审
doi:10.1016/j.lfs.2005.05.005
更新日期:2005-08-19 00:00:00
abstract:AIMS:Ganoderma lucidum, a traditional Chinese medicine, is well known as a modulator of functions of the immune system as well as an anti-tumour agent. However, its active compounds and their molecular mechanisms of action are not well established. GLIS, a proteoglycan isolated from the fruiting body of G. lucidum, sti...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/j.lfs.2010.09.026
更新日期:2010-11-20 00:00:00
abstract:AIMS:There have been recent reports that reconsolidation-based interventions attenuate drug reward memories in rodents. The insular cortex (IC) is an essential part of neural circuits that underlie cue-drug memory reconsolidation. GABAergic interneurons in the IC are a potent control on network excitability and play an...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/j.lfs.2020.117655
更新日期:2020-08-01 00:00:00
abstract::Stonustoxin (SNTX) is a 148 kDa, dimeric, hypotensive and lethal protein factor isolated from the venom of the stonefish Synanceja horrida. SNTX (10-320 ng/ml) progressively causes relaxation of endothelium-intact, phenylephrine (PE)-precontracted rat thoracic aortic rings. The SNTX-induced vasorelaxation was inhibite...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/j.lfs.2007.01.058
更新日期:2007-04-10 00:00:00
abstract:AIMS:Overproduction of nitric oxide (NO) from inducible NO synthase (iNOS) plays many pivotal roles in various inflammatory diseases. In this study, we examined the effects of 6 flavonoids on lipopolysaccharide (LPS)-induced NO generation in macrophage (Mφ), and addressed molecular mechanisms of cardamonin. MAIN METHO...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/j.lfs.2011.06.027
更新日期:2011-08-29 00:00:00
abstract::We hypothesized that the atheroinhibitory and cardioprotective effects of estrogen may be mediated in part through increased prostacyclin formation by the artery wall. Atherosclerotic abdominal aorta was collected at necropsy from ovariectomized female monkeys fed an atherogenic diet alone or with added Premarin. Basa...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/s0024-3205(01)01131-6
更新日期:2001-06-15 00:00:00
abstract:AIMS:C-reactive protein (CRP) is an inflammatory cytokine that has been shown to be an independent predictor of future atherothrombotic events. Hyperactivity of endothelin-1 (ET-1), a potent vasoconstrictor peptide produced by the endothelium, is linked with cardiovascular disease development and progression. ET-1 is s...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/j.lfs.2015.12.030
更新日期:2016-08-15 00:00:00
abstract::Hyperketonemia has been identified as an important factor in diabetic pregnancy affecting growth and development of the offspring. In order to assess the immediate metabolic alterations in embryos caused by excess ketone bodies, we studied rat embryonic neural tissue exposed to a high concentration of beta-hydroxybuty...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/s0024-3205(97)01110-7
更新日期:1998-01-01 00:00:00
abstract::We aimed to study the alterations in serum homocysteine levels and endothelium-dependent and -independent vascular relaxant responses in adjuvant-induced arthritis of the rat and to determine the effects of vitamin E administration on these changes. Arthritis was induced by a single intradermal injection of Freund's c...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/s0024-3205(02)01700-9
更新日期:2002-06-14 00:00:00
abstract:AIMS:The beneficial effects of cannabinoid type 2 receptor (CB2R) activation have been verified in various tissue repair processes. Our recent study revealed CB2R activation promotes myogenesis partly through Nrf2 signaling in a mouse skeletal muscle ischemia-reperfusion (IR) injury model. Other relevant mechanisms nee...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/j.lfs.2020.117989
更新日期:2020-09-01 00:00:00
abstract:AIMS:Cardiovascular disease is the leading cause of death for individuals diagnosed with type II diabetes mellitus (DM). Changes in cardiac function, left ventricular wall thickness and fibrosis have all been described in patients and animal models of diabetes; however, the factors mediating increased matrix deposition...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/j.lfs.2013.01.003
更新日期:2013-03-28 00:00:00
abstract::Psychostimulants including amphetamine and cocaine induce locomotion and stereotypy and suppress eating. Although the capacity of cocaine to alter locomotion is usually viewed as related to dopamine neurotransmission, recent studies suggest that norepinephrine, acting through alpha1-adrenergic receptors (alpha1-ARs) c...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/j.lfs.2007.08.004
更新日期:2007-09-08 00:00:00