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A photoaffinity probe for the cardiac adenosine transporter.

Abstract：

:We have developed and utilized a photoaffinity probe to identify the adenosine transporter in cardiac sarcolemmal (SL) vesicles. The probe is an azidosalicylate derivative of adenosine made by reacting adenosine with N-hydroxysuccinimidyl-4-azidosalicylic acid (NHS-ASA). Following synthesis and radiolabeling of the probe (ASA-adenosine), 125I-ASA-adenosine was purified by high pressure liquid chromatography. Iodine-125-ASA-adenosine, upon irradiation with uv light, covalently labeled a 65 kDa protein in bovine cardiac SL vesicles. Labeling of this protein was greatly diminished in the presence of nonradiolabeled adenosine, 5'-amino adenosine, or guanosine (inhibitors of purine nucleoside transport) but not by glucose (which does not inhibit transport). We conclude that the cardiac adenosine transporter is a protein with an apparent Mr of 65 kDa.

journal_name

Life Sci

journal_title

Life sciences

authors

Hale CC,Rovetto MJ

doi

10.1016/0024-3205(95)00268-b

subject

Has Abstract

pub_date

1995-01-01 00:00:00

pages

245-54

issue

3

eissn

0024-3205

issn

1879-0631

pii

002432059500268B

journal_volume

57

pub_type

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