Abstract:
:The effects of vasoactive intestinal peptide (VIP)-ellipticine (E) derivatives were investigated on breast cancer cells. VIP-ALALA-E and VIP-LALA-E inhibited 125I-VIP binding to MCF-7 cells with an IC(50) values of 1 and 0.2 microM respectively. VIP-ALALA-E and VIP-LALA-E caused elevation of cAMP in MCF-7 cells with ED(50) values of 1 and 0.1 microM. VIP-LALA-E caused increased c-fos mRNA in MCF-7 cells. Radiolabeled VIP-LALA-E was internalized at 37 degrees C and delivered the cytotoxic E into MCF-7 cells. VIP-LALA-E inhibited the clonal growth of MCF-7 cells, decreased cell viability based on trypan blue exclusion and reduced 35S-methionine uptake. These results indicate that VIP-E derivatives function as breast cancer VPAC(1) receptor agonists which inhibit MCF-7 cellular viability.
journal_name
Life Scijournal_title
Life sciencesauthors
Moody TW,Czerwinski G,Tarasova NI,Michejda CJdoi
10.1016/s0024-3205(02)01741-1subject
Has Abstractpub_date
2002-07-19 00:00:00pages
1005-14issue
9eissn
0024-3205issn
1879-0631pii
S0024320502017411journal_volume
71pub_type
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