Inhibition by angiotensin converting enzyme inhibitors of endothelin secretion from cultured human endothelial cells.


:We conducted a study to determine whether angiotensin converting enzyme inhibitors (ACEIs) inhibit endothelin secretion from cultured human endothelial cells. Confluent umbilical vein endothelial cells were incubated in multi-well plates with culture medium containing either captopril (10(-6), 10(-5), 10(-4) M) or enalaprilat (10(-7), 10(-6), 10(-5) M) for 6 hours. Immunoreactive endothelin in the medium was measured by radioimmunoassay. Calf serum (CS) stimulated endothelin release in a concentration-dependent manner, and both ACEIs inhibited 5% CS-stimulated endothelin release in a concentration-dependent manner. To explore the mechanisms of ACEI-induced suppression of endothelin release, the effects of angiotensin II (10(-8), 10(-7), 10(-6) M), angiotensin converting enzyme (0.1, 1, 10 mU/ml), bradykinin (10(-8), 10(-7), 10(-6) M), and sodium nitroprusside (10(-6), 10(-5), 10(-4) M) on endothelin release were also examined. Although angiotensin II and angiotensin converting enzyme had no significant effect on endothelin release, concentration-dependent suppression occurred with bradykinin and sodium nitroprusside. These results indicate that ACEIs inhibit the stimulated release of endothelin from human endothelial cells, and provide indirect evidence that ACEI-induced ET suppression may be mediated via potentiation of autacoid formation from the cells.


Life Sci


Life sciences


Yoshida H,Nakamura M




Has Abstract


1992-01-01 00:00:00














  • Increased endogenous nitric oxide production induced by physical exercise in peripheral arterial occlusive disease patients.

    abstract::The effects of 14-day physical exercise or iloprost treatment (0.5-2 ng/Kg/min) on endogenous nitric oxide production and neutrophil adhesion were evaluated in 20 patients with peripheral arterial occlusive disease (Fontaine Stage II). Peripheral venous blood samples and 4-h urine samples were collected before, immedi...

    journal_title:Life sciences

    pub_type: 临床试验,杂志文章,随机对照试验


    authors: Arosio E,Cuzzolin L,De Marchi S,Minuz P,Degan M,Crivellente F,Zannoni M,Benoni G

    更新日期:1999-01-01 00:00:00

  • Chronic treatment of DA-8159, a new phosphodiesterase type V inhibitor, attenuates endothelial dysfunction in stroke-prone spontaneously hypertensive rat.

    abstract::This study examined the effects of chronic treatment of a new phosphodiesterase type 5 inhibitor, DA-8159, on endothelial dysfunction in stroke-prone spontaneously hypertensive rats (SHR-SP). Six-week-old male SHR-SP were divided into 4 groups; vehicle control, DA-8159 1, 3, and 10 mg/kg/day. During a 32-week experime...

    journal_title:Life sciences

    pub_type: 杂志文章


    authors: Choi SM,Kim JE,Kang KK

    更新日期:2006-02-09 00:00:00

  • Fast-acting excitatory amino acids are involved in the enhancement of the aversiveness of the electrical stimulation of the inferior colliculus by systemic injections of muscimol.

    abstract::Gradual increases in the electrical stimulation of the inferior colliculus produces progressive aversive responses from vigilance, through freezing, until escape. These responses are probably mediated by excitatory amino acids (EAA) mechanisms as microinjection of glutamate into the inferior colliculus can trigger fre...

    journal_title:Life sciences

    pub_type: 杂志文章


    authors: Nobre MJ,Borelli KG,Brandão ML

    更新日期:2002-11-08 00:00:00

  • Age- and sex-dependent stimulation of calcium uptake in duodenal cells by prolactin in vitamin D-deficient rats.

    abstract::Administration of ovine-prolactin (O-PRL) stimulated Ca2+ uptake in isolated duodenal cells prepared from vitamin D-deficient rats. The time course of this effect was biphasic: uptake activity reached a peak in 2.5 hrs followed by a decrease at 5 hrs to original levels. This stimulatory effect of O-PRL was observed in...

    journal_title:Life sciences

    pub_type: 杂志文章


    authors: Balakir RA,Cheng L,Sacktor B,Liang CT

    更新日期:1990-01-01 00:00:00

  • Zonisamide: Antihyperalgesic efficacy, the role of serotonergic receptors on efficacy in a rat model for painful diabetic neuropathy.

    abstract:AIMS:The purpose of this study was to compare the changes of antihyperalgesic effectiveness of zonisamide (25 and 50 mg/kg), an antiepileptic drug, on the early and late phases of neuropathy and to investigate the role of serotonergic descending inhibitory pain pathways in antihyperalgesic effectiveness of zonisamide i...

    journal_title:Life sciences

    pub_type: 杂志文章


    authors: Bektas N,Arslan R,Ozturk Y

    更新日期:2014-01-24 00:00:00

  • Orthovanadate-induced cell death in RET/PTC1-harboring cancer cells involves the activation of caspases and altered signaling through PI3K/Akt/mTOR.

    abstract:AIMS:Oncogenic RET/PTC1 chromosomal rearrangements are hallmarks of thyroid papillary carcinoma. The resulting protein, mainly through tyrosine 451, is responsible for the activation of pathways controlling cell survival, including the PI3K/Akt/mTOR cascade. Vanadium compounds were shown to have anti-neoplastic potenti...

    journal_title:Life sciences

    pub_type: 杂志文章


    authors: Gonçalves AP,Videira A,Soares P,Máximo V

    更新日期:2011-09-12 00:00:00

  • In vivo and in vitro antiinflammatory activity of saikosaponins.

    abstract::Buddlejasaponin I and saikosaponin 1 and 2, biologically active compounds from Scrophularia scorodonia and Bupleurum rigidum respectively, exert potent in vivo antiinflammatory effects on mouse ear edema induced by phorbol myristate acetate (PMA). The effects of these compounds on swelling and other inflammatory param...

    journal_title:Life sciences

    pub_type: 杂志文章


    authors: Bermejo Benito P,Abad Martínez MJ,Silván Sen AM,Sanz Gómez A,Fernández Matellano L,Sánchez Contreras S,Díaz Lanza AM

    更新日期:1998-01-01 00:00:00

  • Antihyperlipidemic properties of beta-pyridylcarbinol. A review of preclinical studies.

    abstract::The available preclinical literature on the antihyperlipidemic properties of beta-pyridylcarbinol is reviewed. Similarities between the pharmacological profiles for beta-pyridylcarbinol and nicotinic acid, and evidence for the metabolic conversion of beta-pyridylcarbinol to nicotinic acid are discussed. Several review...

    journal_title:Life sciences

    pub_type: 杂志文章,评审


    authors: Cohen M

    更新日期:1985-11-25 00:00:00

  • The effects of calorie-matched high-fat diet consumption on spontaneous physical activity and development of obesity.

    abstract:AIMS:To characterize the effects of a calorie matched high-fat diet (HFD) on spontaneous physical activity (SPA), body weight, inflammatory status and expression of genes related to energy homeostasis in hypothalamus of mice. MAIN METHODS:C57Bl/6 mice (n=5 per group) were fed a control diet (16.5% calories from fat) -...

    journal_title:Life sciences

    pub_type: 杂志文章


    authors: Moretto TL,Benfato ID,de Carvalho FP,Barthichoto M,Le Sueur-Maluf L,de Oliveira CAM

    更新日期:2017-06-15 00:00:00

  • No evidence for a peripheral effect of L-DOPA on plasma prolactin in the rat.

    abstract::Male and female rats with two permanently indwelling intravenous catheters were infused for 2 hours with ovine prolactin. During equilibrium conditions the effects of intravenously injected L-DOPA and benzerazide (a blocker of dopa-decarboxylase) on steady state levels of ovine prolactin were measured. A dose of 4.5 m...

    journal_title:Life sciences

    pub_type: 杂志文章


    authors: Mattheij JA,Brouwer GM,Hanstede JG,Swarts JJ

    更新日期:1982-08-30 00:00:00

  • EGFR tyrosine kinase inhibition decreases cardiac remodeling and SERCA2a/NCX1 depletion in streptozotocin induced cardiomyopathy in C57/BL6 mice.

    abstract:AIM:Epidermal growth factor receptor (EGFR) related reactive oxygen species (ROS) generation results in myocardial damage. We aimed to investigate the role of gefitinib (EGFR-tyrosine kinase inhibitor) in diabetic cardiomyopathy (DbCM). METHOD:DbCM was induced by injecting streptozotocin (55 mg/kg for 5 consecutive da...

    journal_title:Life sciences

    pub_type: 杂志文章


    authors: Shah S,Akhtar MS,Hassan MQ,Akhtar M,Paudel YN,Najmi AK

    更新日期:2018-10-01 00:00:00

  • Evidence for two binding sites on membrane-bound angiotensin-converting enzymes (ACE) for exogenous inhibitors except in testis.

    abstract::It was possible to assess that ACE inhibitors bound to two different sites on the enzymes which consist in two homologous domains. Accordingly, it was also shown that testis ACE which consists in a single domain exhibits only one site for inhibitors. The common binding site of both enzymes contains Zn2+, bears the enz...

    journal_title:Life sciences

    pub_type: 杂志文章


    authors: Brée F,Hamon G,Tillement JP

    更新日期:1992-01-01 00:00:00

  • The effect of crebanine on memory and cognition impairment via the alpha-7 nicotinic acetylcholine receptor.

    abstract:AIMS:The aims of the present study were to investigate the effect of crebanine on memory and cognition impairment in mice and to elucidate the underlying molecular mechanisms. MAIN METHODS:The memory-enhancing effects of crebanine were assessed with a water maze test using scopolamine-induced amnesic mice. The molecul...

    journal_title:Life sciences

    pub_type: 杂志文章


    authors: Rojsanga P,Boonyarat C,Utsintong M,Nemecz Á,Yamauchi JG,Talley TT,Olson AJ,Matsumoto K,Vajragupta O

    更新日期:2012-08-21 00:00:00

  • Chemical characterization of the persistent fraction of hydroxyethyl starch in rat serum and spleen.

    abstract::Hydroxyethyl starch (HES) found in rat serum and spleen after single and daily administrations of 0.9 g/kg for 1 week was characterized by gas-liquid chromatography. There was very little difference in the degree of substitution (D.S.) and molar substitution (M.S.) of HES in serum samples obtained at 1 hour and 57 day...

    journal_title:Life sciences

    pub_type: 杂志文章


    authors: Sum CY,Lai CM,Yacobi A,Kalhorn TF

    更新日期:1983-11-14 00:00:00

  • Calcium handling by platelets from normal and malignant hyperthermia-susceptible pigs.

    abstract::Platelets from normal and malignant hyperthermia (MH)-susceptible pigs were evaluated for differences in 45calcium uptake in the absence or presence of caffeine (2-16 mM), halothane (0.05-0.5%), or halothane and caffeine together. There were no statistically significant differences in basal or halothane-inhibited calc...

    journal_title:Life sciences

    pub_type: 杂志文章


    authors: Miller KE,Brooks RR,Bonk KR,Carpenter JF

    更新日期:1991-01-01 00:00:00

  • L-tryptophan attenuation of the dopaminergic and behavioral responses to cocaine.

    abstract::This study assessed the effects of acute intravenous L-tryptophan (neutral amino acid precursor for serotonin) administration on cocaine-induced dopaminergic responses. Male Sprague-Dawley rats were surgically implanted with guide cannulas in the nucleus accumbens 5 days prior to the study and with vascular catheters ...

    journal_title:Life sciences

    pub_type: 杂志文章


    authors: Molina PE,Ahmed N,Gatley J,Volkow ND,Abumrad NN

    更新日期:2001-09-07 00:00:00

  • Contrasting action of antiestrogen (ICI-182780) for preventing initiation of embryo implantation by estradiol or epidermal growth factor (EGF).

    abstract::The pure estrogen antagonist ICI-182780, at doses above 50 micrograms/kg, effectively inhibited the initiation of embryo implantation in rats when administered on day 4 of pregnancy (day 1 = sperm positive). The same dose inhibited the implantation initiating effect of intravenous 25 ng of estradiol-17 beta in delayed...

    journal_title:Life sciences

    pub_type: 杂志文章


    authors: Chatterjee S,Johnson DC

    更新日期:1993-01-01 00:00:00

  • Effects of morphine-3-glucuronide and morphine on the K+-evoked release of [3H]-glutamic acid and [14C]-gamma-aminobutyric acid from rat brain synaptosomes.

    abstract::The effects of morphine-3-glucuronide (M3G) and morphine on the K+-evoked release of [14C]-gamma-aminobutyric acid (GABA) and [3 H]-glutamic acid were investigated in rat brain synaptosomes using superfusion techniques. K+-evoked release of both [14C]-GABA and [3H]-glutamic acid from rat brain synaptosomes was elimina...

    journal_title:Life sciences

    pub_type: 杂志文章


    authors: Bartlett SE,Smith MT

    更新日期:1996-01-01 00:00:00

  • SCH 57790: a novel M2 receptor selective antagonist.

    abstract::As a decrease in cholinergic neurons has been observed in Alzheimer's Disease (AD), therapeutic approaches to AD include inhibition of acetylcholinesterase to increase acetylcholine levels. Evidence suggests that acetylcholine release in the CNS is modulated by negative feedback via presynaptic M2 receptors, blockade ...

    journal_title:Life sciences

    pub_type: 杂志文章


    authors: Lachowicz JE,Lowe D,Duffy RA,Ruperto V,Taylor LA,Guzik H,Brown J,Berger JG,Tice M,McQuade R,Kozlowski J,Clader J,Strader CD,Murgolo N

    更新日期:1999-01-01 00:00:00

  • Effects of morphine on the phenotypic expression of cell surface markers on murine lymphocytes.

    abstract::There is a growing literature indicating that opioid abuse by human addicts and opioid administration to animals have profound effects on the immune system. In the present study, implantation of morphine pellets in mice was associated with reduced phenotypic expression of the cell surface antigens specific to T-lympho...

    journal_title:Life sciences

    pub_type: 杂志文章


    authors: Kimes AS,Smith WJ,Winchell CJ,London ED

    更新日期:1992-01-01 00:00:00

  • Differences of binding characteristics of non-selective opiates towards mu and delta receptor types.

    abstract::[3H]ET (etorphine), which is considered either as an "universal" ligand or a mu agonist, interacts with identical affinities KD = 0.33-0.38 nM to hybrid cells and rabbit cerebellum, pure delta and mu-enriched opioid receptor preparations, respectively. In rat brain tissue, [3H]ET binding is inhibited by DAGO (Tyr-D-Al...

    journal_title:Life sciences

    pub_type: 杂志文章


    authors: Delay-Goyet P,Roques BP,Zajac JM

    更新日期:1987-08-10 00:00:00

  • A comparison of the central and peripheral antimuscarinic effects of atropine and methylatropine injected systemically and into the cerebral ventricles.

    abstract::We compared the relative abilities of atropine sulfate and methylatropine, injected i.v. and into the cerebral ventricles (icv), to block pharmacological responses mediated through central and peripheral muscarinic receptors. The hypotensive response to i.v. injection of acetylcholine (peripheral muscarinic receptors)...

    journal_title:Life sciences

    pub_type: 杂志文章


    authors: Brezenoff HE,Xiao YF,Vargas H

    更新日期:1988-01-01 00:00:00

  • Bleomycin-induced lung injury is enhanced by interferon-alpha.

    abstract::We have evaluated the effect of intraperitoneal (I.P.) injection of human recombinant interferon-2alpha (IFN-alpha) on Bleomycin-induced pulmonary injury in hamsters. Pulmonary injury was induced by a single intratracheal (I.T.) instillation of Bleomycin (Bleo). Six groups of male Syrian hamsters were treated as follo...

    journal_title:Life sciences

    pub_type: 杂志文章


    authors: Berkman N,Goldstein RH,Breuer R

    更新日期:1997-01-01 00:00:00

  • The influence of reproductive hormones on ANF release by rat atria.

    abstract::The effects of subcutaneous (sc) administration of estradiol (50 micrograms/100 microliters/day x 10 day) or testosterone (15 mg/100 microliters twice a week x 2 weeks) on ANF release were examined in isolated perfused rat atria. The concentration of ANF in the perfusate was measured by radioimmunoassay, under basal c...

    journal_title:Life sciences

    pub_type: 杂志文章


    authors: Deng Y,Kaufman S

    更新日期:1993-01-01 00:00:00

  • High affinity binding of serum histidine-rich glycoprotein to nickel-nitrilotriacetic acid: the application to microquantification.

    abstract::Histidine-rich glycoprotein (HRG) is a serum protein with possible pluripotent activities. In this study, a method for the quantification of rabbit histidine-rich glycoprotein (rHRG) was developed based upon the high affinity binding profile of rHRG to nickel-nitrilotriacetic acid (Ni-NTA), an improved chelation agent...

    journal_title:Life sciences

    pub_type: 杂志文章


    authors: Mori S,Takahashi HK,Yamaoka K,Okamoto M,Nishibori M

    更新日期:2003-05-23 00:00:00

  • Changes in the liver protein pattern of female Wistar rats treated with the hypoglycemic agent SDZ PGU 693.

    abstract::SDZ PGU 693 acts as a hypoglycemic agent by stimulating glucose utilisation in insulin-sensitive peripheral tissues, such as skeletal muscle and fat. In a 28 day toxicity study the compound was found to induce hepatocellular hypertrophy in Wistar rats treated with 300 mg/kg/day. To gain insights into the pathomechanis...

    journal_title:Life sciences

    pub_type: 杂志文章


    authors: Arce A,Aicher L,Wahl D,Anderson NL,Meheus L,Raymackers J,Cordier A,Steiner S

    更新日期:1998-01-01 00:00:00

  • Effects of endothelin and vasopressin on portal pressure of rats.

    abstract::Endothelin is a vasoconstrictor peptide which has recently been isolated and sequenced from the vascular endothelial cells. It was reported to increase blood pressure in vivo and produce a prolonged contraction with a slow onset in vitro. The purpose of this study was to investigate whether endothelin can lower the po...

    journal_title:Life sciences

    pub_type: 杂志文章


    authors: Yang MC,Yu PC,Tu MS,Lay CS,Hong CY,Chou CK,Chen CF,Kuo JS

    更新日期:1990-01-01 00:00:00

  • Neurotrophins and the development of cochlear innervation.

    abstract::The rapid progress in the past few years concerning neurotrophic factor research, has greatly stimulated advances in developmental neurobiology of hearing. We have summarized evidence that neurotrophins are expressed by auditory sensory epithelia during the time at which ganglion cells with neurotrophin receptors send...

    journal_title:Life sciences

    pub_type: 杂志文章,评审


    authors: Després G,Romand R

    更新日期:1994-01-01 00:00:00

  • Protection against hydrogen peroxide-induced cytotoxicity in PC12 cells by scutellarin.

    abstract::The present study investigated the protective actions of the antioxidant scutellarin against the cytotoxicity produced by exposure to H2O2 in PC12 cells. This was done by assaying for MTT (3,(4,5-dimethylthiazole-2-yl)2,5-diphenyl-tetrazolium bromide) reduction and lactate dehydrogenase (LDH) release. Reactive oxygen ...

    journal_title:Life sciences

    pub_type: 杂志文章


    authors: Hong H,Liu GQ

    更新日期:2004-04-30 00:00:00

  • Prolyl endopeptidase and dipeptidyl peptidase IV are associated with externalizing and aggressive behaviors in normal and autistic adolescents.

    abstract:AIMS:Peptides and a dysregulated immune system play a role in the pathophysiology of autism. Dysfunctions in prolyl endopeptidase (PEP) and dipeptidyl peptidase IV (DPP-IV) may underpin both the peptidergic and immune alterations in autism. The aims of this study are to: (i) delineate serum PEP and DPP-IV enzyme activi...

    journal_title:Life sciences

    pub_type: 杂志文章


    authors: Frenssen F,Croonenberghs J,Van den Steene H,Maes M

    更新日期:2015-09-01 00:00:00