Abstract：
:The mouse neuroblastoma x rat glioma hybrid NG108-15 was previously shown to express delta opioid receptors. Because neuroblastoma cells display different phenotypes and cloned cell lines are heterogenous, we studied the characteristics and distribution of human 125I-beta-endorphin (125I-beta E) binding sites in cultures of NG108-15 cells with the use of micro-autoradiography and light microscopy. 125I-beta E labeled delta sites in NG108-15 in the presence of the non-opioid blocking peptide, beta-endorphin (6-31) (beta E (6-31)). Silver grains resulting from 125I-beta E binding to the opioid sites occurred in diffuse patches over several cells, with preferential location in dense cell patches. Pretreatment of NG108-15 with the delta agonist DADLE, previously shown to decrease beta E binding to delta sites on intact cells, also reduced silver grain density; however, some cells located in dense cell clusters were resistant to substantial agonist induced loss of labeling. These results suggest that delta opioid binding has a heterogenous cellular distribution in NG108.
journal_name
Life Scijournal_title
Life sciencesauthors
Cone RI,Rosenfeld S,Lameh J,Sadée Wdoi
10.1016/0024-3205(92)90306-asubject
Has Abstractpub_date
1992-01-01 00:00:00pages
PL219-24issue
22eissn
0024-3205issn
1879-0631pii
0024-3205(92)90306-Ajournal_volume
51pub_type
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