Chromatographic approaches to signal and selectivity enhancement for determination of biogenic amines by liquid chromatography with amperometric detection.

abstract：:The aggregation of melanin-granules within fish pigment cells (melanophores) can be elicited either by electrical stimulation of intrinsic nerves or by the addition of adrenergic agonists. The pigment aggregation seems to be mediated by alpha-2-adrenoceptors. In this investigation we have used various agonists and ant...

journal_title：Life sciences

authors： Karlsson JO,Grundström N,Wikberg JE,Friedman R,Andersson RG

更新日期：1985-09-16 00:00:00

abstract：:We have confirmed that hypoxia elicits a substantial rise in blood choline levels in young adult rats. An intravenous infusion of tracer quantities of [2H4]-Ch, serial measurements of blood [2H0]-Ch and [2H4]-Ch, and a simple pharmacokinetic model were used to assess the bidirectional flux of choline between the centr...

journal_title：Life sciences

authors： Jenden DJ,Scremin OU,Roch M,Li G

更新日期：1996-01-01 00:00:00

abstract：AIMS:Transplantation of bone marrow mononuclear cells (BMMCs) exerts neuroprotection against cerebral ischemia. We examined the therapeutic timepoint of allogeneic BMMC transplantation in a rat model of focal cerebral ischemia, and determined the effects of repeated transplantation outside the therapeutic window. MAIN...

journal_title：Life sciences

authors： Kamiya F,Ueda M,Nito C,Kamiya N,Inaba T,Suda S,Saito T,Muraga K,Katayama Y

更新日期：2014-01-24 00:00:00

abstract：:The aim of this study was to verify, by means of functional methods, whether the circadian rhythm changes adrenergic response patterns in the epididymal half of the vas deferens isolated from control rats as well as from rats submitted to acute stress. The experiments were performed at 9:00 a.m., 3:00 p.m., 9:00 p.m.,...

journal_title：Life sciences

authors： Leite-Kassuya CA,Pereira OC

更新日期：2005-08-26 00:00:00

abstract：:Besides an isomer of the cardenolide ouabain, a material with a similar HPLC retention time as ouabain but cross-reactivating with antibodies against the bufadienolide proscillaridin A and inhibiting the sodium pump is known to circulate in human blood plasma (B. SICH et al., Hypertension 27, 1073-1078 (1996).). The c...

journal_title：Life sciences

authors： Li S,Eim C,Kirch U,Lang RE,Schoner W

更新日期：1998-01-01 00:00:00

abstract：:Immunoreactive atrial natriuretic peptide (ir-ANP) was measured in the follicular fluid of pig ovarian follicle, and rabbit ovarian homogenates and perfusates using a specific radioimmunoassay (RIA). Serial dilution curves made with the extracts of follicular fluid, ovarian homogenates and perfusates using SepPak C18 ...

journal_title：Life sciences

authors： Kim SH,Cho KW,Seul KH,Ryu H,Koh GY

更新日期：1989-01-01 00:00:00

abstract：:Bacterial endotoxin or lipopolysaccharide (LPS) can trigger inflammatory responses and cause damage in organs such as liver and lungs when it is introduced into mammals, but the exact molecular events that mediate these responses have remained obscure. In this study, by using 2D gel electrophoresis and cDNA microarray...

journal_title：Life sciences

authors： Xie W,Shao N,Ma X,Ling B,Wei Y,Ding Q,Yang G,Liu N,Wang H,Chen K

更新日期：2006-10-04 00:00:00

abstract：:We had previously hypothesized that linoleic acid (LA) was essential for development of alcoholic induced liver injury in our rat model. Male Wistar rats were fed a nutritionally adequate diet (25% calories as fat) with ethanol (8-17 g/kg/day). The source of fat was tallow (0.7% LA), lard (2.5% LA) or tallow supplemen...

journal_title：Life sciences

authors： Nanji AA,French SW

更新日期：1989-01-01 00:00:00

abstract：BACKGROUND:Coronavirus disease 2019 (COVID-19) has become a severe public health problem around the globe. Various epidemiological, statistical, and laboratory-based studies have shown that the role of temperature and other environmental factors has important influence in the transmission of coronaviruses. Scientific r...

journal_title：Life sciences

authors： Fawad M,Mubarik S,Malik SS,Ren J

更新日期：2021-01-18 00:00:00

abstract：:Momordin II, a ribosome-inactivating protein from Momordica charantia seeds, was purified by a procedure involving a series of chromatographies on S-Sepharose, Sephadex G-50, CM-Sepharose, and Red Sepharose columns. Highly purified momordin II inhibited cell-free protein synthesis, released adenine from rat liver ribo...

journal_title：Life sciences

authors： Valbonesi P,Barbieri L,Bolognesi A,Bonora E,Polito L,Stirpe F

更新日期：1999-01-01 00:00:00

abstract：:Recent studies on the control of adrenal zona glomerulosa function and aldosterone secretion have focussed attention on the role of MSH-like peptides. In particular, at low concentrations, alpha-MSH has a specific stimulatory effect on rat adrenal glomerulosa cells. The synthesis of alpha-MSH analogues which have pote...

journal_title：Life sciences

authors： Vinson GP,Whitehouse BJ,Bateman A,Hruby VJ,Sawyer TK,Darman PS

更新日期：1984-08-06 00:00:00

abstract：AIMS:Endothelin-1 (ET-1) and endothelin-2 (ET-2; Trp(6)Leu(7)ET-1) are expressed by different cell types, but are considered to display identical pharmacological properties on endothelin receptors. We studied agonist-dependent aspects of endothelin(A) (ET(A))-receptor function and the importance of amino acids 6 and 7 ...

journal_title：Life sciences

authors： Compeer MG,Suylen DP,Hackeng TM,De Mey JG

更新日期：2012-10-15 00:00:00

abstract：:The effect of naloxone upon neurologic deficit was evaluated in a model of transient forebrain ischemia in rats. Awake male Wistar rats were subjected to a 30 minute ischemia by occluding both common carotid arteries 8 days after cauterizing vertebral arteries. Administration of naloxone 1 or 5 mg/kg iv 10 minutes aft...

journal_title：Life sciences

authors： Capdeville C,Pruneau D,Allix M,Plotkine M,Boulu RG

更新日期：1986-02-03 00:00:00

abstract：:5-Bromo-2'-deoxyuridine (BrdU) has been recently found to enhance adrenal glucocorticoid secretion in rats. Since BrdU-labeling is today the most employed technique for assessing cell proliferation, we have investigated whether this side-effect of BrdU may influence mitotic activity (as evaluated by metaphase-arrested...

journal_title：Life sciences

authors： Malendowicz LK,Nussdorfer GG,Trejer M

更新日期：1997-01-01 00:00:00

abstract：:We analyzed PGE2 production in primary-cultured human hepatic macrophages (HHM phi) and peripheral monocytes (MO) from patients with and without liver cirrhosis, and correlated PGE2 production with the patients' liver function. Serum choline esterase (ChE) levels were used as an indicator of liver function. PGE2 produ...

journal_title：Life sciences

authors： Funaki N,Arii S,Adachi Y,Higashituji H,Tanaka J,Imamura M

更新日期：1993-01-01 00:00:00

abstract：AIMS:Obesity is associated with increased cardiovascular morbidity and mortality. It is accompanied by augmented O-linked β-N-acetylglucosamine (O-GlcNAc) modification of proteins via increasing hexosamine biosynthetic pathway (HBP) flux. However, the changes and regulation of the O-GlcNAc levels induced by obesity are...

journal_title：Life sciences

authors： Cui YL,Xue RQ,Xi He,Ming Zhao,Yu XJ,Liu LZ,Wu Q,Si Yang,Li DL,Zang WJ

更新日期：2019-04-01 00:00:00

abstract：:Recent studies suggest that protein kinase C and, thus, possibly the rate of inositol phospholipid hydrolysis may regulate the function and distribution of the asialoglycoprotein (or galactosyl) receptor on isolated rat hepatocytes (Takahashi et al., Biochem. Biophys. Res. Commun., 1985, 126, 1054; Fallon and Schwartz...

journal_title：Life sciences

authors： Medh JD,Haynes PA,Weigel PH,LaBelle EF

更新日期：1989-01-01 00:00:00

abstract：:Extracts of Chan Su, a traditional Chinese medication used as a topical anesthetic and cardiac medication, were incubated with cardiomyocytes that had been loaded with a calcium specific fluorescent probe. Calcium transients were measured by real-time fluorescence spectrophotometry following treatment. The transients ...

journal_title：Life sciences

authors： Bick RJ,Poindexter BJ,Sweney RR,Dasgupta A

更新日期：2002-12-27 00:00:00

abstract：OBJECTIVE:The present study aimed to investigate the ability of hesperidin to suppress the migration and invasion of A549 cells, and to investigate the role of the SDF-1/CXCR-4 cascade in this suppression. METHODS:We performed a Transwell migration assay to measure the migratory capability of A549 cells treated with 0...

journal_title：Life sciences

authors： Xia R,Xu G,Huang Y,Sheng X,Xu X,Lu H

更新日期：2018-05-15 00:00:00

abstract：:Neuropeptide Y (NPY) is a 36 amino acid peptide released in central and peripheral mammalian neurons, which appears to contribute to adiposity regulation by increasing food intake, thus promoting weight gain on animals. Nevertheless, little is known about NPY direct actions on white adipocytes. This trial, which was d...

journal_title：Life sciences

authors： Margareto J,Aguado M,Osés-Prieto JA,Rivero I,Monge A,Aldana I,Marti A,Martínez JA

更新日期：2000-11-24 00:00:00

abstract：:The present investigation examined the correlation between the regulation of mucosal prostaglandin (PG) biosynthesis and the release of the neuropeptide substance P (SP) in rat stomachs by indomethacin, a cyclooxygenase inhibitor. When given subcutaneously at the dose of 5 mg/kg, indomethacin reduced mucosal biosynthe...

journal_title：Life sciences

authors： Ko JK,Tang F,Cho CH

更新日期：1997-01-01 00:00:00

abstract：AIMS:Fcγ receptor I (FcγRI/CD64) that is restrictedly expressed on monocytes and macrophages, acts as the single high-affinity receptor of immunoglobulin G (IgG) in human. The expression of FcγRI is positively correlated with immune inflammation. The primary aim of this study was to explore the effects of FcγRI express...

journal_title：Life sciences

authors： Zhang H,Li L,Liu L

更新日期：2018-08-15 00:00:00

abstract：:Heterotrimeric G-protein signalling systems are primarily activated via cell surface receptors possessing the seven membrane span motif. Several observations suggest the existence of other modes of input to such signalling systems either downstream of effectors or at the level of G-proteins themselves. Using a functio...

journal_title：Life sciences

authors： Cismowski MJ,Takesono A,Bernard ML,Duzic E,Lanier SM

更新日期：2001-04-06 00:00:00

abstract：:We previously showed that N1-phenyl-3,5-dinitro-N4,N4-di-n-butylsulfanilamide (denoted GB-II-150) possesses selective antimicrotubule activity against Leishmania donovani and Trypanosoma brucei in vitro [Bhattacharya, G., Herman, J., Delfin, D., Salem, M.M., Barszcz, T., Mollet, M., Riccio, G., Brun, R., Werbovetz, K....

journal_title：Life sciences

authors： Wu D,George TG,Hurh E,Werbovetz KA,Dalton JT

更新日期：2006-08-08 00:00:00

abstract：:Rats treated chronically with the anticancer agent adriamycin (1.5 mg/kg/week X 14 weeks) exhibited cardiac and renal lesions typical of anthracycline toxicity, and had serum hyperlipidemia characterized by 4 to 10 fold elevations in all lipoprotein classes. Heparin-releasable lipoprotein lipase activity measured in p...

journal_title：Life sciences

authors： Thayer WS

更新日期：1985-02-18 00:00:00

abstract：:Muscarinic agonists elicit contraction in the standard guinea pig ileum bioassay through activation of M3 muscarinic receptors that are also linked to phosphoinositide hydrolysis. Surprisingly, the most abundant muscarinic receptor in the ileum is the M2 which causes a specific inhibition of cyclic AMP accumulation el...

journal_title：Life sciences

authors： Ehlert FJ,Thomas EA

更新日期：1995-01-01 00:00:00

abstract：:Tissue copper levels of brindled (Mobr) mice and normal litter-mates after single and repeated dosing with CuCl2 and/or D-penicillamine are examined, together with a study of the cytosol distribution of copper after CuCl2 treatment. The results confirm that the mutant mouse kidney is capable of extensive copper accumu...

journal_title：Life sciences

authors： Hunt DM,Port AE

更新日期：1982-08-02 00:00:00

abstract：:The effects of tetrandrine (TET) on the contractile responses of rat aortic rings and perfused rat mesenteric arteries to phenylephrine (PE) were investigated. TET inhibited the maximal contraction to PE in a concentration-dependent manner. TET significantly inhibited the transient contraction in Ca(2+)-free medium pr...

journal_title：Life sciences

authors： Kwan CY,Chen YY,Ma MF,Daniel EE,Hui SC

更新日期：1996-01-01 00:00:00

abstract：AIMS:Histone deacetylase inhibitors (HDACis) are promising anticancer drugs that open new areas of epigenetic drug discovery. Multiple myeloma (MM) is a malignant tumor of the blood system that is difficult to cure and often relapses. Here, we investigated the in vitro effects of a novel HDACi, LMK-235, on MM cells, an...

journal_title：Life sciences

authors： Li X,Guo Y,Kuang X,Zhao L,Li H,Cheng B,Wang W,Zhang Z,Liu P,Wang J

更新日期：2019-04-15 00:00:00

abstract：AIMS:TRAF6 is an intracellular signal adapter molecule plays a significant role in tumor development. However, the specific mechanism causes and promotes of colorectal cancer keep largely unknown. Therefore, we sought to investigate the roles and the molecular mechanisms of TRAF6 in regulation colorectal cancer. MATER...

journal_title：Life sciences

authors： Zhu G,Cheng Z,Huang Y,Zheng W,Yang S,Lin C,Ye J

更新日期：2019-10-15 00:00:00