Abstract：
AIMS:Hepatocellular carcinoma (HCC) is a leading cause of cancer mortality worldwide. Decrease in NKG2D ligand levels and exhaustion of NK cells in HCC patients are major causes of immune escape, high recurrence, poor prognosis, and low overall survival. Enhancing the susceptibility of HCC to NK cells by upregulating NKG2DLs on tumor cells is an effective treatment strategy. This study aimed to identify the effect of the Anterior gradient 2 (AGR2)-derived peptide P1, which was reported to bind to HLA-A*0201 as an epitope, on both the expression of major histocompatibility complex class I-related chains A/B (MICA/B) on HCC cells and the cytotoxicity of NK cells. MAIN METHODS:The effect of P1 on MICA/B expression on HCC cells was determined by qRT-PCR, western blotting, and flow cytometry analysis. HCC cells were pre-treated with various pathway inhibitors to identify the molecular pathways associated with P1 treatment. The cytotoxicity of NK cells toward HCC was investigated by LDH cytotoxicity assay. The tumor-suppression effect of P1 was determined in vivo using a NOD/SCID mice HCC model. KEY FINDINGS:P1 significantly increased MICA/B expression on HCC cells, thereby enhancing their susceptibility to the cytotoxicity of NK cells in vitro and in vivo. Further, p38 MAPK cell signaling pathway inhibitor SB203580 significantly attenuated the effects of P1 in vivo and in vitro. SIGNIFICANCE:P1 upregulates MICA and MICB expression on HCC cells, thereby promoting their recognition and elimination by NK cells, which makes P1 an attractive novel immunotherapy agent.
journal_name
Life Scijournal_title
Life sciencesauthors
Bian J,He L,Wu Y,Liu W,Ma H,Sun M,Yu J,Yu Z,Wei Mdoi
10.1016/j.lfs.2020.117396subject
Has Abstractpub_date
2020-04-01 00:00:00pages
117396eissn
0024-3205issn
1879-0631pii
S0024-3205(20)30143-0journal_volume
246pub_type
杂志文章相关文献
LIFE SCIENCES文献大全abstract：AIMS:Epidemiological studies report that individuals who exercise are less likely to abuse drugs. Preclinical studies report that exercise, in the form of treadmill or wheel running, reliably decreases the self-administration of psychomotor stimulants and opioids. To date, preclinical studies have only examined the eff...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/j.lfs.2014.08.005
更新日期：2014-10-02 00:00:00
abstract：:The hypothermia induced by apomorphine, a mixed dopamine (DA) agonist in male Swiss-Webster mice, was not blocked by the selective D-1 antagonist SCH 23390 but was completely blocked by the selective D-2 antagonists haloperidol, sulpiride and YM-09151-2. The selective D-1 agonist SKF 38393 did not elicit hypothermic r...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/0024-3205(88)90515-2
更新日期：1988-01-01 00:00:00
abstract：:The conversion of beta-carotene to retinal and the succeeding metabolic process of the retinal leading to production of retinol and retinyl esters are the prerequisite for the utilization of beta-carotene as a provitamin A. These processes are participated by beta-carotene cleavage enzyme, retinal reductase and retino...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/s0024-3205(99)00311-2
更新日期：1999-01-01 00:00:00
abstract：AIMS:Studies have associated obesity with a wide variety of cancers. Metformin, an anti-diabetic drug, has recently received attention as a potentially useful therapeutic agent for treating cancer. Therefore, the objective of this study was to analyze the mechanisms involved in the increase in tumor development and the...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/j.lfs.2014.11.028
更新日期：2015-01-15 00:00:00
abstract：:The effect of nimodipine on nitric oxide synthase (NOS) activities in brains in transient focal cerebral ischemia rats, in cultured mouse neurons and astroglial cells and bovine brain capillary endothelial cells (BCECs) was investigated. The administration of nimodipine (3 mg.kg(-1), p.o., twice a day, for 3 days) bef...
journal_title：Life sciences
pub_type： 杂志文章
doi：
更新日期：1999-01-01 00:00:00
abstract：:This study was designed to investigate the effect of diabetic duration on serum concentrations of endogenous inhibitor of nitric oxide synthase N(G), N(G)-asymmetric dimethylarginine (ADMA) in patients and rats with diabetes, and to determine whether elevated endogenous ADMA is implicated in endothelial dysfunction or...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/j.lfs.2004.10.062
更新日期：2005-05-27 00:00:00
abstract：:Dose-effect curves were obtained for five specific components of stereotyped behavior (locomotor hyperactivity, rearing, sniffing, grooming and oral activity) elicited by the nonselective dopamine agonists N-propylnorapomorphine (NPA; 0.001-4.5 mg/kg) and apomorphine (APO; 0.3-10.0 mg/kg), the selective D2 agonists LY...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/0024-3205(88)90324-4
更新日期：1988-01-01 00:00:00
abstract：:Gastrin is mitogenic for several colon cancers and is postulated as an autocrine growth factor for colon cancer cells. In the present study we report the development of a simple competitive polymerase chain reaction (PCR) method for measuring relative abundance of gastrin gene expression in colon cancer cells. Primers...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/0024-3205(94)00550-8
更新日期：1994-01-01 00:00:00
abstract：:The human eye being a complex and a very sensitive organ makes the drug delivery task challenging. An increase in the intra-ocular pressure at the aqueous humour leads to glaucoma which is not only indecipherable but can also be the reason of blindness for many. The presently available marketed formulations using anti...
journal_title：Life sciences
pub_type： 杂志文章,评审
doi：10.1016/j.lfs.2019.02.029
更新日期：2019-03-15 00:00:00
abstract：:The effects of cloricromene, a coumarine derivative, were studied in an anaesthetized rat model of coronary artery ligation (60 min) followed by reperfusion (60 min; MI/R). Sham operated rats were used as controls (Sham MI/R). Myocardial ischaemia-reperfusion injury produced a marked myocardial injury (necrotic area/a...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/0024-3205(93)90753-p
更新日期：1993-01-01 00:00:00
abstract：:Guinea pig ileum longitudinal muscle/myenteric plexus preparations contain functional mu- and kappa-opioid receptors. A preparation with blocked kappa receptors was obtained by pretreating guinea pigs with 0.1-10.0 mg/kg intraperitoneal doses of a new kappa-selective affinity label, DIPPA (2-(3,4-dichlorophenyl)-N-met...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/s0024-3205(97)00076-3
更新日期：1997-01-01 00:00:00
abstract：:Acute effect of streptozotocin-induced diabetes on several parameters of glucose metabolism was investigated in thymus lymphocytes (thymocytes). The cells from diabetics rats accumulated in vitro about 2-fold more fructose 2,6-bisphosphate (Fru-2, 6-P2) in the presence of increasing glucose concentration than cells fr...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/0024-3205(95)02312-7
更新日期：1996-01-01 00:00:00
abstract：:Effects of ethanol in vitro on membrane vesicles (microsacs prepared from mouse cerebral cortex) were evaluated by monitoring 36Cl- influx. Different assay parameters were tested to determine increased or decreased action of ethanol on GABA-activated chloride channels. The ability of 30 mM ethanol to augment 36Cl- flu...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/0024-3205(90)90009-g
更新日期：1990-01-01 00:00:00
abstract：AIMS:Clinical use of doxorubicin, an effective chemotherapeutic agent, has limited uses due to dose-dependent cardiac toxicity. It has been supposed that the production of free radicals and calcium ions overload can lead to cardiac toxicity. Magnesium is a cardioprotective drug which inhibits lipid peroxidation and red...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/j.lfs.2018.06.022
更新日期：2018-08-15 00:00:00
abstract：:Methotrexate was found to stimulate asparagine synthetase activity in vivo by approximately six-fold in rat liver. The maximum effect of methotrexate on hepatic asparagine synthetase activity was observed sixteen hours after intraperitoneal injection of the drug. Cycloheximide, like methotrexate, is a protein synthesi...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/0024-3205(82)90524-0
更新日期：1982-03-22 00:00:00
abstract：:Acute ethanol treatment in vivo (i.p. injection of 3.5 g ethanol/Kg B. wt.) stimulated the release of beta-endorphin like peptides by the pituitary gland as was indicated by the increased content of beta-endorphin like immunoreactivity (beta-EPLIR) in the plasma. Furthermore, a significant decrease in the anterior lob...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/0024-3205(87)90247-5
更新日期：1987-01-05 00:00:00
abstract：:Kainic acid (KA) is a potent convulsant which, when administered subcutaneously, induces sustained limbic seizures and a pattern of limbic brain damage that is thought to be seizure-mediated. Diazepam suppresses and morphine enhances both the seizures and brain damage induced by KA. Here we show that morphine enhancem...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/0024-3205(82)90582-3
更新日期：1982-07-19 00:00:00
abstract：:The metabolic capability of hepatocytes prepared by the perfusion method (P cells) and the tissue slice method (S cells) has been compared using standardised procedures. Yields of P cells were four times greater than for S cells. Trypan blue exclusion viability and oxygen utilization were similar although the viabilit...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/0024-3205(83)90912-8
更新日期：1983-03-28 00:00:00
abstract：:Hyaluronan (HA) is a multifunctional high molecular weight polysaccharide found throughout the animal kingdom, especially in the extracellular matrix (ECM) of soft connective tissues. HA is thought to participate in many biological processes, and its level is markedly elevated during embryogenesis, cell migration, wou...
journal_title：Life sciences
pub_type： 杂志文章,评审
doi：10.1016/j.lfs.2007.02.037
更新日期：2007-05-01 00:00:00
abstract：AIMS:Previous studies demonstrated that the Western diet (WD), which is rich in saturated fat (HFD) and refined sugar (HSU), is related to the impairments of hippocampus-dependent learning and memory and forebrain synaptic plasticity in rodents. The environmental enrichment (EE) has been shown to enhance learning and m...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/j.lfs.2016.02.011
更新日期：2016-03-01 00:00:00
abstract：UNLABELLED:Isolation of meiotic arrested oocytes from mammalian ovaries involves a resumption of the meiosis. Studying the effect of FSH on this process it was found that doses up to of 0.1 IU/ml did not maintain the meiotic arrest in hamster oocytes in pre-antral follicles during static incubation conditions. However,...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/0024-3205(94)90045-0
更新日期：1994-01-01 00:00:00
abstract：:Sodium spirulan (Na-SP) is a sulfated polysaccharide with M(r) approximately 220,000 isolated from the blue-green alga Spirulina platensis. The polysaccharide consists of two types of disaccharide repeating units, O-hexuronosyl-rhamnose (aldobiuronic acid) and O-rhamnosyl-3-O-methylrhamnose (acofriose) with sulfate gr...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/j.lfs.2003.09.061
更新日期：2004-03-26 00:00:00
abstract：:Previous behavioral studies on triazolam (TZ), which are small in number, could only speculate about tolerance to the anxiolytic effect of TZ, as the experiments did not cover sufficient time (of 4 to 7 days) for tolerance to develop. Therefore longer time for chronic TZ administration is used. We investigated the eff...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/s0024-3205(03)00612-x
更新日期：2003-10-31 00:00:00
abstract：:Gastric mucosal lipid peroxide levels, based on the amounts of thiobarbituric acid reacting substances, increased soon after oral application of absolute ethanol. On the other hand, gastric mucosal nonprotein sulfhydryl levels slightly but significantly decreased. Administration of 20% ethanol, a mild irritant which c...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/0024-3205(86)90216-x
更新日期：1986-06-09 00:00:00
abstract：:In order to examine possible selectivity of amine uptake by synaptic vesicles, the ATP-stimulated accumulation of 3H-5-hydroxytryptamine (5HT) by synaptic vesicles from rat whole brain was examined in a medium comprised largely of membrane-impermeant anions (d-tartrate). Such media have previously been shown to stabil...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/0024-3205(86)90174-8
更新日期：1986-03-31 00:00:00
abstract：:Continuous three day administration via implanted minipumps of cocaine hydrochloride (50-450 mg/kg/day, sc and 100-250 mg/kg/day, iv) did not produce axonal degeneration in frontal agranular cortex or neostriatum that was detectable by Fink-Heimer silver staining or tyrosine hydroxylase immunolabeling. This is in cont...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/0024-3205(88)90307-4
更新日期：1988-01-01 00:00:00
abstract：:The effects of capsaicin on cellular growth and intracellular calcium mobilization were examined in human cervical carcinoma derivation, HeLa cells. Capsaicin inhibited cellular growth and increased intracellular calcium level in HeLa cells. This capsaicin-induced intracellular calcium concentration rise was blocked b...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/s0024-3205(99)00046-6
更新日期：1999-01-01 00:00:00
abstract：:A laboratory mouse can be partially restrained by placing it inside a cage with its tail protruding through a hole in one wall and taped to a surface outside of the cage. The mouse maintains enough mobility to perform an operant response yet its movements are limited to the extent that electrical or chemical stimulati...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/0024-3205(82)90164-3
更新日期：1982-11-15 00:00:00
abstract：:This study was designed to determine whether the increase in atriopeptin secretion induced by an intravenous infusion of norepinephrine is mediated directly by adrenergic receptor stimulation or indirectly by the associated increase in atrial pressure. Norepinephrine was infused at 0.5 microgram.kg-1.min-1 for 30 min ...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/0024-3205(90)90046-t
更新日期：1990-01-01 00:00:00
abstract：:The localization of vasoactive intestinal polypeptide (VIP) in the male genitourinary tract was investigated in the rabbit and man by means of radioimmunoassay and immunohistochemistry. In addition, the in vitro effect of VIP upon penile smooth muscle from man, the Vervet monkey, and the rabbit was investigated. Signi...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/s0024-3205(83)80013-7
更新日期：1983-07-25 00:00:00
