Abstract:
:More than 140 thousands of women suffer from endometrial carcinoma in the worldwide, and over 40 thousand of the patients die before and after in surgery and chemoradiotherapy because of its metastasis. However, its molecular mechanism is much less known compared to other cancers. In this study, we demonstrated that long non-coding RNA PCAT1 is dramatically increased in the tissues and plasma from endometrial carcinoma (EC) (n = 100, all p < 0.001) controlled by its paracancerous tissue, and cell lines including RL-952, HEC-1-B, KLE, Ishikawa, and AN3CA compared to the cells from normal endometrium (all p < 0.001). When lncRNA PCAT1 was knocked-down, the KLE and AN3CA cells exhibited slow capability on proliferation and colony formation in vitro. With the silence of lncRNA PCAT1, the cells were markedly inhibited on migration and invasion in vitro (all p < 0.001), which were confirmed on the EC patient subjects. When expressions of lncRNA PCAT1 were interfered in the cells, expressions of E-cadherin but not N-cadherin and Vimentin were significantly promoted with a strong up-regulation accompanied by nearly completed recoveries on migration and invasion (all p < 0.001). In order to analyze the association of lncRNA PCAT1 and E-cadherin, we silenced the expressions of both genes and unveiled that EC migration and invasion were significantly congested (all p < 0.001). Importantly, we found that the E-cadherin down-regulation caused by lncRNA PCAT1 associates with histone methyltransferase EZH2. When over-expression of EZH2 was applied in the PCAT1 silenced cells, the expression of E-cadherin experienced significant decrease in the cell lines. Reversely, when expression of EZH2 was annulled in the PCAT1 silenced cells, the expression of E-cadherin was significantly boosted in the cells (all p < 0.001). Furthermore, the interaction of lncRNA PCAT1 and EZH2 were approved with immunoprecipitation. Our data demonstrated that the methyltransferase EZH2 related up-regulation of lncRNA PCAT1 along with down-regulation of E-cadherin could be essential in oncogenesis of endometrial carcinoma in both EC cells and patient subjects. These compact data suggest that combination of lncRNA PCAT1, EZH2 and E-cadherin could provide valued information for efficient EC diagnostics, which would propose a potential target for EC treatment with EZH2i on methyltransferation.
journal_name
Life Scijournal_title
Life sciencesauthors
Zhang C,Shao S,Zhang Y,Wang L,Liu J,Fang F,Li P,Wang Bdoi
10.1016/j.lfs.2020.117295subject
Has Abstractpub_date
2020-02-15 00:00:00pages
117295eissn
0024-3205issn
1879-0631pii
S0024-3205(20)30042-4journal_volume
243pub_type
杂志文章相关文献
LIFE SCIENCES文献大全abstract::The validity of a photoelectric method for continuous cerebral blood volume (CBV) measurement was tested and modified for the rat's brain. A new way of introducing a miniature light source between the two hemispheres and fixing a light sensitive silicone blue cell to the outer surface of the parietal bone was develope...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/0024-3205(86)90163-3
更新日期:1986-11-03 00:00:00
abstract::Embryonic heart development is a very complicated process regulated precisely by a network composed of many genes and signaling pathways in time and space. Forkhead box (Fox, FOX) proteins are a family of transcription factors characterized by the presence of an evolutionary conserved "forkhead"or "winged-helix" DNA-b...
journal_title:Life sciences
pub_type: 杂志文章,评审
doi:10.1016/j.lfs.2015.12.001
更新日期:2016-01-01 00:00:00
abstract::Comparison of neuronal and non-neuronal membrane transport of, and neuroexcitation by, the dicarboxylic amino acids bring out provocative similarities in structural selectivity, and hence in the strategies for studying them. Parallel anomalies in stereoselectivity show for both phenomena that the recognition sites are...
journal_title:Life sciences
pub_type: 杂志文章,评审
doi:10.1016/0024-3205(83)90258-8
更新日期:1983-12-05 00:00:00
abstract::After 7 years from its cloning, the transient receptor potential vanilloid type-1 (TRPV1) channel remains the sole membrane receptor mediating the pharmacological effects of the hot chilli pepper pungent component, capsaicin, and of the Euphorbia toxin, resiniferatoxin. Yet, this ion channel represents one of the most...
journal_title:Life sciences
pub_type: 杂志文章,评审
doi:10.1016/j.lfs.2005.05.021
更新日期:2005-08-19 00:00:00
abstract::Intracerebroventricularly (i.c.v.)-administered [D-Ala2]deltorphin II (20 micrograms) produced a marked locomotor hyperactivity in male ICR mice. The locomotor hyperactivity induced in response to i.c.v. [D-Ala2]deltorphin II (20 micrograms) was suppressed by pretreatment with naltriben (NTB, 10 micrograms) but not 7-...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/0024-3205(96)00307-4
更新日期:1996-01-01 00:00:00
abstract::The mechanism of fibrate-induced myopathy was investigated in this report. When clofibrate (30 to 300 microM) was applied to L6 rat skeletal myoblasts, dose-dependently apoptosis was observed within 24 h. In the apoptotic myoblasts, a caspase-12 cleavage was observed at 2 h and with following caspases-9 and -3-related...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/j.lfs.2005.08.003
更新日期:2006-03-13 00:00:00
abstract::The effect of nimodipine on nitric oxide synthase (NOS) activities in brains in transient focal cerebral ischemia rats, in cultured mouse neurons and astroglial cells and bovine brain capillary endothelial cells (BCECs) was investigated. The administration of nimodipine (3 mg.kg(-1), p.o., twice a day, for 3 days) bef...
journal_title:Life sciences
pub_type: 杂志文章
doi:
更新日期:1999-01-01 00:00:00
abstract::The effects of three local anesthetics, lidocaine, dibucaine, and tetracaine, on Na+/H+ antiporter activity were examined in brush border membrane-reconstituted vesicles. Lidocaine at 10 microM inhibited H+ efflux in the presence of an inward Na+ gradient, suggesting that this anesthetic specifically inhibits the Na+/...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/0024-3205(90)90172-n
更新日期:1990-01-01 00:00:00
abstract::The antinociceptive and temperature responses to morphine were compared in male and female rats from two different strains. Males of both the Sprague-Dawley and Wistar-Furth strains were slightly more responsive to the acute actions of morphine than were females of the same strain. However, Wistar-Furth animals requir...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/0024-3205(84)90633-7
更新日期:1984-04-23 00:00:00
abstract:AIMS:MicroRNA-1 (miR-1) has been demonstrated as a tumor-suppressive miRNA, which shows a down-regulated pattern in several human malignancies including hepatocellular carcinoma (HCC). However, the pathophysiologic roles of miR-1 and their mechanisms in HCC tumorigenesis are still not totally elucidated. MAIN METHODS:...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/j.lfs.2012.08.015
更新日期:2012-10-05 00:00:00
abstract::Effects of scratching behavior on dermatitis, transepidermal water loss (TEWL) and serum IgE concentrations were examined in NC/Nga (NC) mice with toenails (WIT) and without toenails (WOT). The first study was a preventive treatment done to cut off hind toenails before dermatitis induction and the second study was a t...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/j.lfs.2004.07.022
更新日期:2004-12-31 00:00:00
abstract::Human, dog and rabbit corpus cavernosum type 5 phosphodiesterases (PDE5) were isolated and their characteristics were compared. The three enzymes showed Km values of 0.8, 2.1 and 2.3 uM, respectively. They exhibited similar pH-dependence with optimal pH being 7.5. They required Mg++ for activity and the activity was s...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/s0024-3205(01)00989-4
更新日期:2001-03-16 00:00:00
abstract::In adult male Wistar rats submitted to a standardized noise stress, intravenous TRH induced a prolactin (PRL) secretory response. Prior IV naloxone administration not only lowered plasma PRL levels in those stressed rats but abolished also the stimulatory action of TRH. This effect was further studied by superfusion e...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/0024-3205(87)90756-9
更新日期:1987-06-22 00:00:00
abstract::We recently labeled with carbon-11, a high affinity, selective, 5-HT3 receptor (5-HT3R) ligand, S21007, for potential positron emission tomography (PET) applications. To evaluate the in vivo binding properties of [11C]S21007, its brain regional distribution, tissue and plasma pharmacokinetics and plasma metabolisation...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/s0024-3205(97)01058-8
更新日期:1998-01-01 00:00:00
abstract::The intestinal mucosa of infant rats was found to produce ketones when incubated in Krebs-Ringer-Bicarbonate solution. No production was found in weaned rats. Ketogenesis could be inhibited by D-carnitine or tetradecylglycidic acid (TDGA) an inhibitor of long-chain acylcarnitine transferase, suggesting that ketone pro...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/0024-3205(87)90718-1
更新日期:1987-09-21 00:00:00
abstract::Even though conventional systemic doses of cimetidine and other histamine H(2) antagonists display minimal brain penetration, central nervous system (CNS) effects (including seizures and analgesia) have been reported after administration of these drugs in animals and man. To test the hypothesis that cimetidine-like dr...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/j.lfs.2004.05.020
更新日期:2004-10-08 00:00:00
abstract:AIMS:Ventilatory disturbance is a fatal side-effect of opioid analgesics. Separation of analgesia from ventilatory depression is important for therapeutic use of opioids. It has been suggested that opioid-induced ventilatory depression results from a decrease in adenosine 3',5'-cyclic monophosphate content in the respi...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/j.lfs.2015.02.006
更新日期:2015-04-15 00:00:00
abstract:AIMS:Tumor necrosis factor-related apoptosis-inducing ligand (TRAIL) has been considered to be one of the most promising candidates in research on treatments for cancer, including renal cell carcinoma (RCC). However, many cells are resistant to TRAIL-induced apoptosis which limits the potential application of TRAIL in ...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/j.lfs.2014.02.002
更新日期:2014-04-01 00:00:00
abstract::The effects of delta 9-THC and other cannabinoids on cAMP levels in synaptosomes from mouse brains were investigated in order to determine whether cannabinoids produced their behavioral effects through alterations in adenylate cyclase. delta 9-THC (0.01-10 microM) did not significantly alter basal cAMP levels, whereas...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/0024-3205(91)90450-p
更新日期:1991-01-01 00:00:00
abstract:AIM:Cyclophosphamide (CYP) chemotherapy induces bladder toxicity and hemorrhagic cystitis in cancer patients constituting a current clinical concern. Oxidative inflammatory cascades have been implicated as the mechanism contributing to CYP bladder urotoxicity. We thus assayed to explore whether zinc (Zn) supplementatio...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/j.lfs.2020.118913
更新日期:2021-02-01 00:00:00
abstract::Quinolinic acid (QUIN) levels are elevated in patients and animals suffering from chronic infectious diseases. In the present study, male Sprague-Dawley rats were used to test the anti-inflammatory effects of QUIN using the carrageenan (CGN)-induced paw edema assay and the CGN sponge assay. Results of these studies in...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/s0024-3205(99)00049-1
更新日期:1999-01-01 00:00:00
abstract::Several cryopreservation methods for precision-cut rat liver slices (PCLS) have been proposed, allowing a short-term (a few hours) maintainance of viability and functionality upon thawing. The aim of the present study was to test the metabolic capacity of PCLS cryopreserved by an ultrarapid method. The biotransformati...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/s0024-3205(01)01031-1
更新日期:2001-04-13 00:00:00
abstract:AIMS:Multiple sclerosis (MS) is one of the commonest neurologic disorders globally. LncRNA OIP5-AS1 has been found to be implicated in the etiology of MS. This study was to explore the roles and molecular mechanisms of OIP5-AS1 in the development of MS. MATERIALS AND METHODS:RT-qPCR assay was used to measure expressio...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/j.lfs.2021.119108
更新日期:2021-01-27 00:00:00
abstract::Since the late 1960's, radioenzymatic assays have gradually come to replace the less sensitive and less specific spectrofluorometric and bioassay procedures previously used to determine many of the neurotransmitters. These assays provide the means to measure picogram quantities of most of these substances, and have en...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/0024-3205(87)90185-8
更新日期:1987-08-17 00:00:00
abstract::Anorexia, nausea/emesis and peripheral sensorial neuropathy are frequent adverse effects associated with chemotherapy. Cannabinoids have been proposed to alleviate these effects, but their preventive properties in long-term experimental models have not been tested. This study was conducted to determine whether or not ...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/j.lfs.2007.06.012
更新日期:2007-07-19 00:00:00
abstract:AIM:Nuclear factor-kappa B inhibitor alpha (IκBα) polymorphisms were found to be associated with inflammatory diseases. However, the association between IκBα polymorphisms with gastric cancer is still unknown. We aim to investigate the association between IκBα polymorphisms and gastric cancer risk in a large population...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/j.lfs.2011.02.016
更新日期:2011-04-25 00:00:00
abstract::In vitro studies showed that organophosphate insecticides have different IC50 values (i.e., concentration of inhibitor required to inhibit 50% enzyme activity) for acetylcholinesterases (AChE's) from different species. Since previous reports indicated that the binding of active cholinesterase inhibitors to non-critica...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/0024-3205(82)90426-x
更新日期:1982-07-12 00:00:00
abstract::Previous investigations showed that chronic metabolic acidosis (CMA) increased the paracellular permeability of ion and neutral hydrophilic molecules in the duodenum of rats and small intestinal-like cell lines. Since proteins of the claudin family have been known to regulate the paracellular transport in several epit...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/j.lfs.2007.01.063
更新日期:2007-04-17 00:00:00
abstract::Quetiapine, a new atypical antipsychotic drug, has beneficial effects on cognitive impairment and neuropathological changes in treating chronic neurodegenerative diseases. Our previous studies have demonstrated that quetiapine may have neuroprotective properties. In the present study, we investigated the effects of a ...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/j.lfs.2007.05.020
更新日期:2007-07-12 00:00:00
abstract::Muscarinic receptors on epithelial cells mediate intestinal secretion, while those in intestinal smooth muscle mediate motility. Experiments were carried out to determine whether the muscarinic receptors mediating each of these two functions in intestinal tissue might be associated with differences in the way agonist ...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/0024-3205(85)90444-8
更新日期:1985-05-20 00:00:00