Abstract:
AIMS:Cancer Stem Cells (CSCs) refers to heterogeneous tumor cells retaining the abilities of self-renewal and differentiation. This study used mRNAsi, which is an index to describe the similarity between tumor cells and CSCs, to define genes involved in endometrial carcinoma. MATERIALS AND METHODS:The mRNA expression profiles of 552 tumor samples and 23 non-tumor samples were calculated for differentially expressed genes. WGCNA was utilized to construct gene co-expression networks and classify screened genes into different modules. Univariate and multivariate Cox regression models were performed to identify and construct the prognostic model. Time-dependent receiver operating characteristic (ROC), Kaplan-Meier curve, multivariate Cox regression analysis, and nomogram were used to assess the prognostic capacity of the six-gene signature. The screened genes were further validated by GEO (GSE17025) and qRT-PCR in EC tissues. KEY FINDINGS:2573 upregulated and 1890 downregulated genes were identified. A total of 35 genes in the turquoise module were identified as key genes. With multivariate analysis, six genes (DEPDC1, FAM83D, NCAPH, SPC25, TPX2, and TTK) up-regulated in endometrial carcinoma were identified, and their higher expression was associated with a higher stage/age/grade. Moreover, ROC and Kaplan-Meier plots indicated these genes had a high prognostic value for EC. A nomogram was constructed for clinical use. In addition, we explored the pathogenesis involving six genes. The results showed that these genes may become pathogenic as their copy numbers changes and methylation level reduces. Finally, GSEA revealed these genes had a close association with cell cycle, etc. SIGNIFICANCE: These findings may provide new insights into the treatment of diseases.
journal_name
Life Scijournal_title
Life sciencesauthors
Liu J,Wu Z,Sun R,Nie S,Meng H,Zhong Y,Nie X,Cheng Wdoi
10.1016/j.lfs.2020.118231subject
Has Abstractpub_date
2020-10-01 00:00:00pages
118231eissn
0024-3205issn
1879-0631pii
S0024-3205(20)30983-8journal_volume
258pub_type
杂志文章相关文献
LIFE SCIENCES文献大全abstract::Bradykinin receptor stimulation results in G-protein-coupled phospholipase activation, initiating protein kinase C (PKC) stimulation and cytosolic free Ca2+ concentration ([Ca2+]i) rises as signalling pathways. Using Rb+ as a tracer for K+, we have studied the mechanisms involved in bradykinin-stimulated Rb+ influx in...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/s0024-3205(96)00530-9
更新日期:1996-01-01 00:00:00
abstract::Biphasic cutaneous reaction with peak response at 1 (early phase) and 24 to 48 hour (late phase) was elicited by epicutaneous challenge with antigen in actively and passively sensitized mice. Mice were actively immunized with dinitrophenylated (DNP) ascaris antigen and challenged with dinitrofluorobenzene (DNFB). Pass...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/0024-3205(94)00859-0
更新日期:1994-01-01 00:00:00
abstract::The present report investigated whether percentages of circulating natural killer (NK) cells and NK cell activity (NKCA) are associated with psychological variables. Subjects (n = 95) were selected, based on a combination of low or high scores on questionnaires on daily hassles and self-reported symptoms, to create fo...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/0024-3205(93)90002-k
更新日期:1993-01-01 00:00:00
abstract::The influence of different, nitric oxide-containing ruthenium complexes on the evoked potentials recorded from the CA1 region of the mouse hippocampus in vitro has been investigated. Of the compounds tested, only trans-[(NO)(P(OEt)3)(NH3)4Ru](PF6)3 (1-2.5 mM) exerted a strong facilitatory action on the population spik...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/s0024-3205(01)00951-1
更新日期:2001-02-16 00:00:00
abstract::Extracts of plasma obtained from adult humans before and immediately after physical stress were subjected to high pressure liquid chromatography (HPLC); extracts or HPLC fractions, resp., were analysed for the presence of human beta-endorphin (beta H-endorphin) or fragments thereof by several radioimmunoassays using a...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/0024-3205(83)90451-4
更新日期:1983-01-01 00:00:00
abstract::Noradrenaline is taken up in noradrenergic neurones via a desipramine-sensitive transport system (uptake1). There are reports which describe the activity of uptake1 being increased by extracellular ATP and ATP gamma S. This was attributed to a protein kinase-mediated mechanism. We reevaluated this phenomenon since a m...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/0024-3205(94)00665-2
更新日期:1994-01-01 00:00:00
abstract::There is evidence that senescence affects neurotransmission at different levels. In particular, this review summarizes the studies on age-dependent modifications in protein phosphorylation, which represents the final pathway in the action of transmitters and hormones at neuronal level. Cyclic AMP-dependent protein kin...
journal_title:Life sciences
pub_type: 杂志文章,评审
doi:10.1016/0024-3205(91)90492-t
更新日期:1991-01-01 00:00:00
abstract::Previous studies from this laboratory have shown that rats with experimental cirrhosis of the liver induced by the combined administration of oral phenobarbital and inhaled carbon tetrachloride show an hyperdynamic status with enhanced cardiac output (CO), and decreased mean arterial pressure (MAP) and peripheral vasc...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/0024-3205(86)90531-x
更新日期:1986-07-21 00:00:00
abstract:AIMS:Ischemia-reperfusion (I/R) event in vascular and nervous system has been documented to rising ischemic and vasculitic neuropathic pain, clinically resembles the complex regional pain syndrome (CRPS). The present study evaluated the effect of montelukast, a cysteinyl leukotriene receptor (Cys-LTC(4) and Cys-LTD(4))...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/j.lfs.2012.03.010
更新日期:2012-05-22 00:00:00
abstract:AIMS:Insulinoma-associated protein 2β (IA-2β) is considered to play a significant role in regulated secretion. Recent studies have shown that the mouse brain expresses three major isoforms of IA-2β, named IA-2β60, IA-2β64, and IA-2β71. In this study, we analyzed the tissue-, cell- and organelle-specific distributions o...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/j.lfs.2011.02.018
更新日期:2011-04-25 00:00:00
abstract::Central dopaminergic (DA) function in children was assessed by monitoring plasma-free homovanillic acid (pHVA) levels after brief (18 hour) administration with debrisoquin sulfate, a peripherally active antihypertensive agent that blocks peripheral, but not central, HVA production. Brief debrisoquin administration res...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/0024-3205(86)90239-0
更新日期:1986-03-17 00:00:00
abstract::Synthesis and degradation of polyphosphoinositides in a rat brain synaptosome preparation were depressed by phenobarbital. Phosphatidylinositol-4-phosphate kinase (PIP-kinase), the enzyme which synthesizes phosphatidylinositol-4,5-bisphosphate (PIP2) was most strongly affected (50% inhibition at 3 mM phenobarbital); p...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/0024-3205(87)90530-3
更新日期:1987-11-02 00:00:00
abstract:AIMS:Changes in cardiac autonomic nervous function have been evaluated by studying the related indexes of heart rate variability (HRV) in patients with focal epilepsy (FE) in the interictal period. MAIN METHODS:A total of 30 FE patients who were treated in our department from July 2015 to May 2017, were included into ...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/j.lfs.2018.08.033
更新日期:2018-09-15 00:00:00
abstract::3H-Flunitrazepam (FNZP) binding was examined in a crude membrane fraction obtained from rat interscapular brown adipose tissue (IBAT). A single population of binding sites was apparent with dissociation constant (KD) = 0.47 +/- 0.04 microM and maximal number of binding sites (Bmax) = 31 +/- 5 pmol.mg prot-1. From the ...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/0024-3205(88)90077-x
更新日期:1988-01-01 00:00:00
abstract::Since hypertension may compromise the ability to withstand hypernatremic dehydration, we investigated the impact of two experimental models of hypertension and pharmacologic normalization of blood pressure on the tolerance to chronic hypernatremic dehydration. In DOCA-salt hypertensive animals and the spontaneously-hy...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/0024-3205(90)90450-6
更新日期:1990-01-01 00:00:00
abstract::Neurodegenerative diseases need the drugs to be delivered right inside the brain to maximizing the therapeutic effects. This can be achieved by use of novel targeted delivery systems such as nanoemulsions. Nanoemulsions (NE) are nano-sized emulsions that are manufactured for enhancing the delivery of drugs to the targ...
journal_title:Life sciences
pub_type: 杂志文章,评审
doi:10.1016/j.lfs.2020.117394
更新日期:2020-03-15 00:00:00
abstract::To analyze the effects of diabetes mellitus on the vascular responsiveness to nitric oxide and thromboxane receptor stimulation, 2 mm long segments of basilar, coronary, renal and tail arteries from male and female, control (normoglycemic) and streptozotocin-induced diabetic rats, were prepared for isometric tension r...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/s0024-3205(02)02444-x
更新日期:2003-02-14 00:00:00
abstract::The extended infection with Helicobacter pylori (H. pylori), one of the most frequent infectious agents in humans, may cause gastritis, peptic ulcers, gastric mucosa-associated lymphoid tissue (MALT) lymphoma, and gastric cancer. During H. pylori infection, different kinds of inflammatory cells such as dendritic cells...
journal_title:Life sciences
pub_type: 杂志文章,评审
doi:10.1016/j.lfs.2019.116688
更新日期:2019-08-15 00:00:00
abstract::The new Coronavirus (SARS-CoV-2) is the cause of a serious infection in the respiratory tract called COVID-19. Structures of the main protease of SARS-CoV-2 (Mpro), responsible for the replication of the virus, have been solved and quickly made available, thus allowing the design of compounds that could interact with ...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/j.lfs.2020.117963
更新日期:2020-09-01 00:00:00
abstract::In order to clarify the role played by interleukin-2 (IL-2) in the regulation of fibroblast function, we investigated the effect of rat IL-2 and human recombinant IL-2 on 3T3 fibroblast proliferation and collagen synthesis. Fibroblasts were incubated with various concentrations of IL-2 for different periods of time. I...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/0024-3205(96)00125-7
更新日期:1996-01-01 00:00:00
abstract:AIMS:The purpose of this study was to investigate mechanisms of chronic alcohol-induced cardiac remodeling and dysfunction. We also sought to determine the role of cardiac fibroblasts, which play a dynamic role in cardiac remodeling, in mediating these effects. MAIN METHODS:Adult male Wistar rats were exposed to ethan...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/j.lfs.2020.117330
更新日期:2020-03-15 00:00:00
abstract::Nitric oxide (NO) is generated from L-arginine by different isoforms of the enzyme nitric oxide synthase (NOS) and is known to be involved in mediating several biological functions, some of which are associated with reproduction. In this study, we examined the ability of the prepubertal ovary to express the inducible ...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/s0024-3205(97)00710-8
更新日期:1997-01-01 00:00:00
abstract::The discovery and development of new drugs targeting voltage-gated ion channels are important for treating a variety of medical conditions and diseases. Ion channels are molecular nanostructures expressed ubiquitously throughout the whole body, and are involved in many basic physiological processes. Over the years, na...
journal_title:Life sciences
pub_type: 杂志文章,评审
doi:10.1016/j.lfs.2011.04.020
更新日期:2011-10-10 00:00:00
abstract:AIMS:Endothelial dysfunction is a key triggering event in the development of cardiovascular diseases and the current study explored this phenomenon in the context of inflammation, apoptosis, reactive oxygen species (ROS) and the phosphatidylinositol 3-kinase (PI3K)/Akt signaling pathway during chronic diabetes. MAIN M...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/j.lfs.2010.03.019
更新日期:2010-06-05 00:00:00
abstract::Effects of amphetamine on concentrations of dopamine, norepinephrine and serotonin in several monoamine-containing cell body and terminal regions were examined left and right separately in rats. Results suggest that amphetamine reduced the L-R asymmetry of most of these measures, and this effect is more significant in...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/0024-3205(87)90334-1
更新日期:1987-04-06 00:00:00
abstract::The binding of different gangliosides to rat T-helper lymphocytes was characterized under conditions that decrease CD4 expression on different mammalian T-helper lymphocytes. Saturation binding by monosialylated [3H]-GM1 to rat T-lymphocytes was time- and temperature-dependent, had a dissociation constant (KD) of 2.2 ...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/0024-3205(89)90123-9
更新日期:1989-01-01 00:00:00
abstract::The ontogenetic development of [3H]-spiroperidol binding sites was measured in the optic tectum, cerebellum, forebrain base, and forebrain roof of 1-, 4-, and 16-day-old chicks. In the chick optic tectum and cerebellum both the density and the total number of [3H]-spiroperidol binding sites increased from 4- to 16-day...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/0024-3205(89)90331-7
更新日期:1989-01-01 00:00:00
abstract:AIMS:The hypoxia-stimulated response of the endocrine system depends on the kind and duration of hypoxia. Hypoxia has been reported to stimulate testosterone (T) production in rats, but the mechanisms remain to be investigated. MATERIALS AND METHODS:Male rats were divided into two groups. The rats exposed to chronic i...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/j.lfs.2019.116694
更新日期:2019-09-15 00:00:00
abstract::The somatostatin antagonist analogue cyclo Ahep-Phe-D-Trp-Lys-Thr(Bzl) stimulates growth in young female rats, while somatostatin itself has only equivocal effects on inhibiting growth. ...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/0024-3205(85)90621-6
更新日期:1985-07-08 00:00:00
abstract::The effect of nimodipine on nitric oxide synthase (NOS) activities in brains in transient focal cerebral ischemia rats, in cultured mouse neurons and astroglial cells and bovine brain capillary endothelial cells (BCECs) was investigated. The administration of nimodipine (3 mg.kg(-1), p.o., twice a day, for 3 days) bef...
journal_title:Life sciences
pub_type: 杂志文章
doi:
更新日期:1999-01-01 00:00:00