Abstract：
:The colchicine-induced accumulation of vasopressin (AVP) and oxytocin (OXT) has recently been applied to estimate the synthesis and turnover rates for these neuropeptides in whole rat hypothalamus. In the present studies, this pharmacologic procedure has been examined as a potential method for estimating hypothalamic somatostatin (SRIF) synthesis rate, and evaluated further for its utility in estimating nonapeptide synthesis in individual hypothalamic nuclei. Adult male rats received a single injection of colchicine (8 micrograms) into the third ventricle under pentobarbital anesthesia. Twenty-four hr later, immunoreactive (IR) levels of AVP and OXT increased considerably, as previously noted. Hypothalamic IR-SRIF levels, however, were unaffected. The absolute increases in IR-AVP and IR-OXT were greatest in the supraoptic nucleus (SON), with smaller increments in the para/periventricular hypothalamus (PVH) and the median eminence (ME). IR-SRIF levels showed no changes in the PVH or the ME. As a test, the method was applied to the detection of changes in AVP synthesis in diabetic rats. The colchicine procedure reported increases in AVP synthesis in both the SON and PVH in diabetic animals, a result compatible with that obtained previously for whole hypothalamus using radiolabeled procedures. Together, the results indicate that the colchicine procedure is useful in detecting changes in the syntheses of some (AVP and OXT) but not all (SRIF) neuropeptides, and that when applicable, the method is sufficiently sensitive to detect changes in small hypothalamic regions. The method may prove useful in estimating changes in peptide synthesis analogous to that used for serotonin and dopamine; e.g., 5-hydroxytryptophan and dopa accumulation following inhibition of aromatic L-amino acid decarboxylase.
journal_name
Life Scijournal_title
Life sciencesauthors
Liu B,Kwok RP,Fernstrom JDdoi
10.1016/0024-3205(91)90441-dsubject
Has Abstractpub_date
1991-01-01 00:00:00pages
345-52issue
5eissn
0024-3205issn
1879-0631pii
0024-3205(91)90441-Djournal_volume
49pub_type
杂志文章相关文献
LIFE SCIENCES文献大全abstract：:Decentralization of the superior cervical ganglion (S.C.G.) of the rat elevated the neuropeptide-Y immunoreactivity (NPY-ir) content of the ganglion on day 1 (+43%) but not on day 3 post-surgery. The content of NPY-ir in the iris was increased by decentralization (+40%) 3 days post-surgery, and treatment with clonidin...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/0024-3205(88)90333-5
更新日期：1988-01-01 00:00:00
abstract：:Peroxisome proliferator-activated receptor (PPAR) and retinoid x receptor (RXR) play important roles in fatty acid metabolism. The present study examined the regulation of retinoic acid receptor (RAR alpha, beta, and gamma), RXR (alpha, beta, and gamma), PPAR, cytochrome P450 2E1 (CYP2E1), catalase, and beta-actin gen...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/0024-3205(94)00953-8
更新日期：1995-01-01 00:00:00
abstract：:Short-chain fatty acids (SCFA) are produced by fermentation of water-soluble fiber by anaerobic bacteria in the large bowel. Fiber-rich diets decrease the risk of developing inflammatory bowel disease (IBD) and butyrate enemas are effective as a therapy in some patients. Crohn's disease, one form of IBD, appears to in...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/s0024-3205(03)00490-9
更新日期：2003-08-15 00:00:00
abstract：AIMS:Tianeptine is an atypical antidepressant drug that has a different mechanism of action than other antidepressants. Olanzapine is an atypical antipsychotic drug used for the treatment of schizophrenia. The present study was undertaken to investigate effects of chronic administration of tianeptine or olanzapine on u...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/j.lfs.2012.09.023
更新日期：2012-12-17 00:00:00
abstract：:Nitric oxide (NO) may play an important regulatory role in airway function. We have, thus, investigated in vitro whether epithelium derived NO may modulate cholinergic neurotransmission, via release of NO in guinea pig trachea, by using L-arginine (L-ARG), a precursor of NO synthesis, and L-N(G)-nitro-arginine-methyl-...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/0024-3205(95)00053-9
更新日期：1995-02-17 00:00:00
abstract：:The effects of methylprednisolone (m-PSL) on IL-1beta-induced neutrophil-endothelial cell interactions, which are normally mediated by increased expression of both intercellular adhesion molecule-1 (ICAM-1) and E-selectin on endothelial cells, were examined using an in vitro flow system. Human neutrophilic polymorphon...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/s0024-3205(97)00290-7
更新日期：1997-01-01 00:00:00
abstract：:PACAP did not affect secretory activity of dispersed rat adrenocortical cells, but it markedly raised aldosterone (ALDO) and corticosterone (B) production by adrenal slices, containing both medullary and cortical tissues. The secretagogue effects of PACAP were suppressed by PACAP(6-38), a specific competitive antagoni...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/0024-3205(94)00423-p
更新日期：1995-01-01 00:00:00
abstract：AIM:To evaluate the therapeutic efficacy of hypoxic cell-sensitizer Sanazole (SAN) -directed targeting of cytotoxic drug Berberine (BBN) and Iron-oxide nanoparticle (NP) complexes, to solid tumor in Swiss albino mice. MAIN METHODS:NP-BBN-SAN complexes were characterized by FTIR, XRD, TEM and Nano-size analyzer. This c...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/j.lfs.2017.12.036
更新日期：2018-02-15 00:00:00
abstract：AIMS:The purpose of the study was to determine whether late therapeutic hypothermia (LTH), administered after reperfusion, could prevent adverse left ventricular (LV) remodeling and improve cardiac function in the rat myocardial ischemia/reperfusion model. MAIN METHODS:Rats were randomized to normothermia (n = 10) or ...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/j.lfs.2019.04.026
更新日期：2019-07-15 00:00:00
abstract：:The effect of clotrimazole on Ca2+ signaling in Madin Darby canine kidney (MDCK) cells was investigated by using fura-2 as a Ca2+ indicator. Clotrimazole (1-30 microM) induced a concentration-dependent [Ca2+]i increase. The [Ca2+]i increase comprised an initial rise and a slow decay. External Ca2+ removal partly inhib...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/s0024-3205(00)00558-0
更新日期：2000-01-01 00:00:00
abstract：UNLABELLED:The mechanisms by which exercise affects atherosclerotic plaque stability remain incompletely understood. We evaluated the effects of two training protocols on both atherosclerotic plaque structure and the signaling pathways involved in plaque rupture. METHODS:Male low-density lipoprotein (LDL) receptor kno...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/j.lfs.2016.04.007
更新日期：2016-05-15 00:00:00
abstract：:Rats implanted subcutaneously with an empty osmotic pump connected by a polyethylene catheter to a jugular vein for 5 to 10 days evinced a decreased splenocyte responsiveness to blastogenic stimulation in vitro to bacterial lipopolysaccharide, a known B cell stimulator, as well as to the plant mitogens pokeweed mitoge...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/0024-3205(92)90291-v
更新日期：1992-01-01 00:00:00
abstract：:Tumor angiogenesis and lymphangiogenesis are key features of tumor progression and metastasis. The role of tumor cells-derived factors in the promotion of associated angiogenesis and lymphangiogenesis is much studied and, no doubt, very important for the understanding of cancer progression. This review aims to present...
journal_title：Life sciences
pub_type： 杂志文章,评审
doi：10.1016/j.lfs.2012.10.008
更新日期：2013-02-07 00:00:00
abstract：AIMS:With the improvement of the survival rates in children acute lymphoblastic leukemia (ALL), some children ALL survivors show impaired cognitive function. Methotrexate (MTX), an essential component in ALL treatment, has been reported to be related to neurologic sequelae and to increased oxidative stress through its ...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/j.lfs.2019.116931
更新日期：2020-02-01 00:00:00
abstract：:Alpha-lipoic acid (ALA), a potent biological antioxidant, improves insulin action of skeletal muscle glucose transport and metabolism in both human and animal models of insulin resistance. In order to obtain further insight into the potential intracellular mechanisms for the action of ALA on insulin-stimulated glucose...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/s0024-3205(97)00562-6
更新日期：1997-01-01 00:00:00
abstract：:Clinical trials with muscarinic agonists or acetylcholine esterase inhibitors for the treatment of Alzheimer's dementia have shown disappointing or equivocal results. An alternative treatment of this disease is the development of drugs which enhance the release of acetylcholine. It is believed, that of the five muscar...
journal_title：Life sciences
pub_type： 杂志文章,评审
doi：10.1016/0024-3205(93)90307-o
更新日期：1993-01-01 00:00:00
abstract：AIMS:7-Ketocholesteryl-9-carboxynonanoate (oxLig-1), a lipid moiety of oxidized low-density lipoprotein (oxLDL), has been reported to be a crucial ligand of beta2-glycoprotein I (β(2)-GPI), and plays a potential role in the development of atherosclerosis (AS), however, the role of the sole oxLig-1 in the development of...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/j.lfs.2010.09.028
更新日期：2010-11-20 00:00:00
abstract：:Dietary guidelines published in the past two decades have acknowledged the beneficial effects of myricetin, an important and common type of herbal flavonoid, against several human diseases such as inflammation, cardiovascular pathologies, and cancer. An increasing number of studies have shown the beneficial effects of...
journal_title：Life sciences
pub_type： 杂志文章,评审
doi：10.1016/j.lfs.2015.10.004
更新日期：2015-12-01 00:00:00
abstract：:Prenatal exposure to low concentrations of carbon monoxide (CO, 75 and 150 ppm from day 0 to day 20 of gestation), resulting in maternal blood HbCO concentrations equivalent to those maintained by human cigarette smokers, leads to subtle myelin alterations in the sciatic nerve of male rat offspring. The rapid growth s...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/s0024-3205(00)00761-x
更新日期：2000-08-25 00:00:00
abstract：AIM:Although constitutive autophagy is linked to redox state and participates in cell homeostasis, it is scarcely known if redox state, autophagy, and lysosomal function depend on sex, a factor that largely influences health and diseases. Therefore, we evaluated the existence of sex differences in redox state and const...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/j.lfs.2013.06.001
更新日期：2013-08-06 00:00:00
abstract：AIMS:Oxidative stress emerges after reperfusion of an organ following an ischemic period and results in tissue damage. In the heart, an amplified generation of reactive oxygen species and a significant Ca(2+) accumulation cause ventricular arrhythmias and mitochondrial dysfunction. This occurs in consequence of increas...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/j.lfs.2013.12.026
更新日期：2014-02-06 00:00:00
abstract：:We find a significantly lower level of specific [3H]nicotine binding in the fetal rat forebrain of 20 day gestational age as compared to adult rat tissue, and a progressive increase in the [3H]nicotine binding in the early neonatal forebrain, reaching adult level at 14-28 days of age. The maximal binding sites (Bmax) ...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/0024-3205(86)90057-3
更新日期：1986-02-17 00:00:00
abstract：:Two groups of individuals, 26 normotensive normolipemic and 37 normotensive hyperlipemic, all without family history of hypertension have been selected in attempt to demonstrate whether Li-Na countertransport of erythrocytes is influenced by plasma and membrane lipid composition. The maximal rate of Li-Na countertrans...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/0024-3205(87)90637-0
更新日期：1987-08-31 00:00:00
abstract：:Ischemic preconditioning (IP) may protect the lung from ischemia-reperfusion (I/R) injury following cardiopulmonary by-pass and lung or heart transplantation. The present study was undertaken to investigate the role of ATP-dependent potassium channels (K(ATP)) in IP in the isolated buffer-perfused rat lung (IBPR) unde...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/j.lfs.2006.07.011
更新日期：2006-11-02 00:00:00
abstract：:Polyamines have been shown to relax several types of smooth muscle including vasculature. In the estrogen-treated uterus, uterine blood flow and polyamine levels in the uterus are increased. The relaxant effect of polyamines on blood vessels suggest that polyamines may act on uterine vasculature to cause uterine hyper...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/0024-3205(92)90446-v
更新日期：1992-01-01 00:00:00
abstract：:This study was performed in order to investigate the type of T cells recruited to the blood in response to an acute bout of exercise with regard to mean lengths of telomeric terminal restriction fragments (TRF) and surface activation markers and with special emphasis on age-associated differences. Ten elderly and ten ...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/s0024-3205(99)00531-7
更新日期：1999-01-01 00:00:00
abstract：AIMS:Data suggest pharmacological treatment of depression with selective serotonin reuptake inhibitors (SSRI) may impair bone health. Our group has previously modeled compromised craniofacial healing after treatment with sertraline, a commonly prescribed SSRI, and hypothesized potential culprits: alterations in bone ce...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/j.lfs.2020.117827
更新日期：2020-08-15 00:00:00
abstract：:A sensitive and specific radioimmunoassay for substance P has been developed and its full characterisation is described. The assay was used to investigate the stability of substance P in tissue in order to establish the optimal conditions of tissue storage for maximal recovery of the peptide. The results indicate unex...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/0024-3205(83)90211-4
更新日期：1983-02-07 00:00:00
abstract：AIM:Preparation of pegylated gold nanorods (PEG-AuNRs) that are capable of converting near infrared (NIR) light into heat. Evaluation of cancer therapeutic efficacy and long-term toxicity of the proposed photothermal therapy in comparison with other conventional modalities. MATERIALS AND METHODS:Prepared PEG-AuNRs wer...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/j.lfs.2020.118108
更新日期：2020-09-15 00:00:00
abstract：:Brown adipose tissue (BAT) has multiple functions in the human body, including the production of heat and increasing energy consumption. However, BAT is also related to many kinds of diseases, such as obesity and metabolic disorders. The progression of such diseases occurs at the cellular level, and thus, imaging tech...
journal_title：Life sciences
pub_type： 杂志文章,评审
doi：10.1016/j.lfs.2020.117500
更新日期：2020-05-15 00:00:00
