Abstract：
:The effect of clotrimazole on Ca2+ signaling in Madin Darby canine kidney (MDCK) cells was investigated by using fura-2 as a Ca2+ indicator. Clotrimazole (1-30 microM) induced a concentration-dependent [Ca2+]i increase. The [Ca2+]i increase comprised an initial rise and a slow decay. External Ca2+ removal partly inhibited the Ca2+ signals by reducing both the initial rise and the decay phase, indicating that clotrimazole triggered both Ca2+ influx and Ca2+ release. Pretreatment with 30 microM clotrimazole in Ca2+-free medium abolished the Ca2+ release induced by thapsigargin (1 microM), an endoplasmic reticulum Ca2+ pump inhibitor, and conversely, pretreatment with thapsigargin prevented clotrimazole from releasing more Ca2+. This suggests that the thapsigargin-sensitive Ca2+ store is the source of clotrimazole-induced Ca2+ release. Clotrimazole (10 microM) triggered Mn2+ quench of fura-2 fluorescence which was partly inhibited by 1 mM La3+. Addition of 3 mM Ca2+ induced a [Ca2+]i increase after preincubation with 10 microM clotrimazole in Ca2+-free medium, indicating that clotrimazole activated capacitative Ca2+ entry. However, 10 and 30 microM clotrimazole inhibited 1 microM thapsigargin-induced capacitative Ca2+ entry by 21% and 74%, respectively. Pretreatment with 40 microM aristolochic acid to inhibit phospholipase A2 reduced 30 microM clotrimazole-induced Ca2+ release by 51%, but inhibiting phospholipase C with 2 microM U73122 had little effect. This implies that clotrimazole induces Ca2+ release in an IP3-independent manner, which could be modulated by phospholipase A2-coupled events.
journal_name
Life Scijournal_title
Life sciencesauthors
Jan CR,Tseng CJ,Chou KJ,Chiang HTdoi
10.1016/s0024-3205(00)00558-0subject
Has Abstractpub_date
2000-01-01 00:00:00pages
2289-96issue
23eissn
0024-3205issn
1879-0631pii
S0024320500005580journal_volume
66pub_type
杂志文章相关文献
LIFE SCIENCES文献大全abstract：:Electrically evoked release of dopamine from the caudate nucleus is reduced by the dopamine receptor agonists, apomorphine and bromocriptine, and facilitated by neuroleptic drugs, which act as dopamine autoreceptor antagonists. The potencies of chlorpromazine, fluphenazine, levomepromazine and their hydroxy-metabolite...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/0024-3205(90)90088-9
更新日期：1990-01-01 00:00:00
abstract：AIMS:The antihypertensive mechanism (s) of the epigallocatechin-3-gallate (EGCG), a major effective component in green tea, might associate with microRNAs (miRNAs). Here, we aimed to investigate which microRNA in aorta of spontaneously hypertensive rats (SHRs) were modulated by administration of EGCG and its mechanism....
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/j.lfs.2018.04.041
更新日期：2018-06-15 00:00:00
abstract：AIM:Renin-angiotensin system has a role in inflammation and also involves in learning and memory. In the present study, the effects of captopril on lipopolysaccharide (LPS) induced learning and memory impairments, hippocampal cytokine levels and brain tissues oxidative damage was investigated. MATERIALS AND METHODS:Th...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/j.lfs.2016.10.026
更新日期：2016-12-15 00:00:00
abstract：:We provide direct evidence that verapamil inhibits active digoxin secretion in renal tubular cells (LLC-PK1), and that verapamil increases cellular accumulation of digoxin. These findings suggest that verapamil inhibits the digoxin active secretory transport at the apical membranes, supporting the theory that P-glycop...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/0024-3205(93)90667-r
更新日期：1993-01-01 00:00:00
abstract：:The level of hepatic lipidperoxides in chick embryos was determined during cataract formation resulting from glucocorticoid treatment. When 15 day old chick embryos were administered 0.25 mumol of hydrocortisone acetate their hepatic lipidperoxide level, determined by thio-barbituric acid, increased after a lag time o...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/0024-3205(84)90664-7
更新日期：1984-08-27 00:00:00
abstract：:The concentrations and molecular forms of urinary and plasma gastrin from normal subjects were studied by radioimmunoassays using two region-specific antisera. Urinary concentration of NH2-terminal big gastrin (G-34) immunoreactivity was several hundred times as great as that of COOH-terminal gastrin immunoreactivity....
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/0024-3205(90)90112-5
更新日期：1990-01-01 00:00:00
abstract：AIMS:Interleukin-1β (IL-1β) is a cytokine that is known to activate the stress axis and suppress the reproductive axis. Different brain areas are involved in the regulation of these two axes. However, they are both under the stimulatory control of the catecholamine, norepinephrine (NE). Here, we hypothesized that IL-1β...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/j.lfs.2012.09.004
更新日期：2012-10-29 00:00:00
abstract：:Recent studies have indicated that Ginsenoside Rb1, one of the major components of ginseng root, may play an important role in protecting cells from damage. Here, we investigated the neuroprotective activity of Rb1 after hypoxic injury in young rats. About 50% animals were dead by exposing hypoxic condition three time...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/j.lfs.2004.10.011
更新日期：2005-01-14 00:00:00
abstract：:The recovery rate of striatal dopamine receptors following blockade by N-ethoxycarbonyl-2-ethoxy-1, 2-dihydroquinoline (EEDQ) in vivo is reduced by 25-35% in striata from senescent Wistar rats when compared to mature counterparts. No differences in binding affinity for [3H]-spiperone were observed for the different ag...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/0024-3205(84)90416-8
更新日期：1984-08-20 00:00:00
abstract：:Clinical trials with muscarinic agonists or acetylcholine esterase inhibitors for the treatment of Alzheimer's dementia have shown disappointing or equivocal results. An alternative treatment of this disease is the development of drugs which enhance the release of acetylcholine. It is believed, that of the five muscar...
journal_title：Life sciences
pub_type： 杂志文章,评审
doi：10.1016/0024-3205(93)90307-o
更新日期：1993-01-01 00:00:00
abstract：:Two recently isolated peptides, endomorphin-1 (Tyr-Pro-Trp-Phe-NH2) and endomorphin-2 (Tyr-Pro-Phe-Phe-NH2), are highly selective micro-opioid receptor agonists with analgesic actions in the tail-flick test. To further assess the analgesic properties of these peptides, the effects of endomorphin-1, endomorphin-2, and ...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/s0024-3205(00)00689-5
更新日期：2000-07-14 00:00:00
abstract：:Cystic fibrosis (CF) remains a major problem in human genetics and cell pathophysiology. It is a single gene trait caused by a mutation on the long arm of chromosome 7. Among its expressions are abnormal regulation of chloride channels and/or microobstructions in exocrine tissues. Here, evidence is presented that mito...
journal_title：Life sciences
pub_type： 杂志文章,评审
doi：10.1016/0024-3205(89)90389-5
更新日期：1989-01-01 00:00:00
abstract：:The effects of taurine on hepatic cholesterol metabolism were investigated in hamsters fed a high-fat diet or normal chow. Two weeks-treatment of taurine at 1% in drinking water prevented high-fat diet-induced increase in cholesterol levels of serum and liver. The decrease in serum cholesterol by taurine was due to de...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/s0024-3205(02)01507-2
更新日期：2002-04-05 00:00:00
abstract：AIMS:Aristolochic acid (AA) nephrotoxicity is related to accumulation of methylglyoxal (MGO) and N(ε)-(carboxymethyl)lysine (CML) in the mouse kidney. We studied the activity of renal semicarbazide-sensitive amine oxidase (SSAO), a key enzyme involved in MGO generation, in AA-treated mice, and investigated nephroprotec...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/j.lfs.2014.07.034
更新日期：2014-09-26 00:00:00
abstract：:Growth of P. aeruginosa, slowed by the addition of monofluoromethylornithine, difluoromethylarginine and dicyclohexylammonium sulfate, could be restored by addition of 0.1 mM putrescine plus 0.1 muM spermidine, or 0.1 mM spermidine or 5 mM putrescine by themselves. Lower concentrations of putrescine (0.1 mM - 1 mM) al...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/0024-3205(84)90605-2
更新日期：1984-04-16 00:00:00
abstract：:The aim of this study was to investigate whether the A1/A2 receptor agonist, 5'-N-ethylcarboxamidoadenosine (NECA), and the selective A1 agonist, N6-cyclopentyladenosine (CPA), induced physical dependence by quantifying specific antagonist-precipitated withdrawal syndromes in conscious rats. In addition, the presence ...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/s0024-3205(01)01155-9
更新日期：2001-07-06 00:00:00
abstract：AIM:Up-regulation of inflammasome proteins was reported in dystrophin-deficient muscles. However, it remains to be determined whether inflammasome activation plays a role in the pathogenesis of Duchenne muscular dystrophy. This study was therefore set out to investigate whether genetic disruption of the inflammasome pa...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/j.lfs.2020.118069
更新日期：2020-09-15 00:00:00
abstract：:Gastrin is mitogenic for several colon cancers and is postulated as an autocrine growth factor for colon cancer cells. In the present study we report the development of a simple competitive polymerase chain reaction (PCR) method for measuring relative abundance of gastrin gene expression in colon cancer cells. Primers...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/0024-3205(94)00550-8
更新日期：1994-01-01 00:00:00
abstract：:The association between various parameters of acute and chronic smoking status and plasma levels of three proteins, C9, C1-inhibitor (C1-INH) and alpha 1-protease inhibitor (alpha 1-PI) were determined for 49 male cigarette smokers and 49 age-matched nonsmokers (mean age = 32.2 years). The mean number of cigarettes sm...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/0024-3205(85)90391-1
更新日期：1985-03-11 00:00:00
abstract：:The somatostatin antagonist analogue cyclo Ahep-Phe-D-Trp-Lys-Thr(Bzl) stimulates growth in young female rats, while somatostatin itself has only equivocal effects on inhibiting growth. ...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/0024-3205(85)90621-6
更新日期：1985-07-08 00:00:00
abstract：:In the present study the formation of thrombin-antithrombin-complexes (TAT) during incubation of thrombin (0.89, 4.5, 8.9 nmol/l) and antithrombin (4.6 micromol/l) on the surface of cultured human EC, derived from different parts of the circulation, and on the surface of human vessel segments was studied. In the absen...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/s0024-3205(98)00416-0
更新日期：1998-01-01 00:00:00
abstract：:The present study is undertaken to investigate whether the phospholipase A(2) (PLA(2)) influences mRNA nucleocytoplasmic transport evaluated by nucleoside triphosphatase (NTPase) activity and mRNA export in isolated hepatic nuclear envelope. Isolated hepatic nuclei from rat liver were exposed to PLA(2) (10(-5) approxi...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/s0024-3205(03)00357-6
更新日期：2003-07-11 00:00:00
abstract：:Cortical activity during motor task performance is attenuated in individuals with Parkinson disease (PD) relative to age-matched adults without PD, and this activity is enhanced with antiparkinson medication. It remains unclear, however, whether the relative change in cortical activity over the duration of the task, i...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/j.lfs.2018.03.019
更新日期：2018-05-01 00:00:00
abstract：:We examined the effect of beta-alanyl-L-histidinato zinc (AHZ) on the differentiation of human periodontal ligament (HPDL) cells. HPDL cells were cultured with alpha-minimum essential medium containing 10% fetal bovine serum with or without 10(-4) or 10(-5) M AHZ for up to 10 days. The expression of runt-related trans...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/j.lfs.2003.09.069
更新日期：2004-04-02 00:00:00
abstract：AIMS:Insulin-like growth factor (IGF)-1 is a major mitogenic growth factor for mesangial cells (MCs). Statins slow the progression of chronic kidney disease by affecting inflammatory cell signaling pathways, in addition to improving lipid profile, however, no studies have investigated the effects of fluvastatin on mito...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/j.lfs.2009.02.022
更新日期：2009-05-22 00:00:00
abstract：:Rats treated chronically with the anticancer agent adriamycin (1.5 mg/kg/week X 14 weeks) exhibited cardiac and renal lesions typical of anthracycline toxicity, and had serum hyperlipidemia characterized by 4 to 10 fold elevations in all lipoprotein classes. Heparin-releasable lipoprotein lipase activity measured in p...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/0024-3205(85)90167-5
更新日期：1985-02-18 00:00:00
abstract：AIMS:Absent in melanoma 2 (AIM2) is a cytosolic DNA sensor which plays an important role in inflammasome formation and is involved in various cellular functions including pyroptosis, fibrosis, and tissue injury. Our study aimed to investigate whether AIM2 plays a role in diabetic cardiomyopathy (DCM) and to explore its...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/j.lfs.2019.02.035
更新日期：2019-03-15 00:00:00
abstract：:Adenylate cyclase activity in pancreatic acinar cell membranes was determined in rats that had undergone a treatment with pentagastrin (250 micrograms/kg, intraperitoneal three times daily) for 1 week or that had undergone small bowel resection (90%) and were sacrified at 2 weeks, 1 month and 6 months after interventi...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/0024-3205(95)02226-9
更新日期：1995-01-01 00:00:00
abstract：AIMS:The mechanisms underlying the changes in blood-brain barrier (BBB) integrity and the generation of seizures in childhood associated with preexisting brain lesions like cortical dysplasia (CD) are poorly understood. We investigated the effects of levetiracetam (LEV) on BBB integrity and the survival during hyperthe...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/j.lfs.2010.09.014
更新日期：2010-11-20 00:00:00
abstract：:In canine renal tubular cells, the effect of Y-24180, a presumed specific platelet activating factor (PAF) receptor antagonist, on intracellular Ca(2+) concentration ([Ca(2+)](i)) was measured by using fura-2 as a Ca(2+)-sensitive fluorescent probe. Y-24180 (0.1-10 microM) caused a rapid and sustained [Ca(2+)](i) rise...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/j.lfs.2003.09.033
更新日期：2004-01-02 00:00:00
