Abstract：
AIMS:Aristolochic acid (AA) nephrotoxicity is related to accumulation of methylglyoxal (MGO) and N(ε)-(carboxymethyl)lysine (CML) in the mouse kidney. We studied the activity of renal semicarbazide-sensitive amine oxidase (SSAO), a key enzyme involved in MGO generation, in AA-treated mice, and investigated nephroprotective effects produced by metformin, a MGO scavenger. METHODS:Mice were orally administered water or metformin for 15 days (12 or 24 mg kg(-1)day(-1)), and injected AA (5mgkg(-1)day(-1)) intraperitoneally for 8 days starting on day 8. Renal function was studied, and histopathological examination, determination of renal SSAO activity, and measurement of MGO levels were performed. KEY FINDINGS:Compared to control mice, AA-injected mice showed significant renal damage and approximately 2.7-fold greater renal SSAO activity (p<0.05). Further, compared to control treatment, administration of 12 mg/kg metformin inhibited formation of renal lesions, and significantly decreased renal MGO levels (37.33 ± 9.78 vs. 5.89 ± 2.64 μg/mg of protein, respectively, p<0.01). In the AA-treated mice, metformin also inhibited the accumulation of CML in renal tubules, but did not affect SSAO activity. SIGNIFICANCE:This study is the first to show elevated renal SSAO activity in AA-treated mice, which could be involved in MGO accumulation. Moreover, MGO scavenging by metformin reduces AA nephrotoxicity. These findings suggest that reducing MGO accumulation produces nephroprotection, revealing new therapeutic strategies for the management. SSAO is a key enzyme involved in MGO generation, and consequently, inhibition of renal SSAO activity is worth investigating in AA nephrotoxicity and other renal pathologies further.
journal_name
Life Scijournal_title
Life sciencesauthors
Huang TC,Chen SM,Li YC,Lee JAdoi
10.1016/j.lfs.2014.07.034subject
Has Abstractpub_date
2014-09-26 00:00:00pages
4-11issue
1eissn
0024-3205issn
1879-0631pii
S0024-3205(14)00655-9journal_volume
114pub_type
杂志文章相关文献
LIFE SCIENCES文献大全abstract：:Groups of 35-day-old male C57BL/6 mice were stressed 1 hour per day by immobilization for 1, 2, 3, 5, 8, 11 or 14 consecutive days. Control groups were left undisturbed. The animals were then killed and body weight and the weights of the thymus, spleen and axillary lymph nodes determined. Chronic immobilization stress...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/s0024-3205(97)00606-1
更新日期：1997-01-01 00:00:00
abstract：AIMS:In the oviduct, muscarinic acetylcholine receptors (MR) are linked with motility regulation and nicotinic receptors (nAChR) with ectopic pregnancy. We here aimed to determine the repertoire of cholinergic receptor expression in the murine oviduct and their functional coupling to regulation of intracellular calcium...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/j.lfs.2012.03.016
更新日期：2012-11-27 00:00:00
abstract：:Mucosal mast cells are implicated in visceral hypersensitivity associated with irritable bowel syndrome (IBS). In this study, we investigated the role of mast cells in the development of visceral hypersensitivity by using mast cell deficient (Ws/Ws) rats and their control (W+/W+). In W+/W+ rats, an injection of 2,4,6-...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/j.lfs.2007.11.027
更新日期：2008-02-13 00:00:00
abstract：:Transport of tyrosine (Tyr) and phenylalanine (Phe) across the rat nasal mucosa was studied using an in situ perfusion technique. It was found that both amino acids were absorbed by active, saturable transport processes. The Km and Vmax values were calculated to be 0.68 mM and 0.44 mM/hr for L-Tyr, and 0.40 mM and 0.3...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/0024-3205(88)90062-8
更新日期：1988-01-01 00:00:00
abstract：:The intracellular ionic content of human erythrocytes may be altered by hyperglycaemia. Despite this, very little is known about the cellular mechanisms linking glucose and cellular magnesium homeostasis. We measured intracellular ionized magnesium in human lymphocytes, by means of a fluorimetric technique, total intr...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/s0024-3205(02)01992-6
更新日期：2002-09-20 00:00:00
abstract：:The influence of prior incubation on [3H]tryptamine binding was investigated in rat brain synaptic plasma membranes. A 55 min preincubation of the membranes at 37 degrees C induced an approx. 2.4-fold increase in the specific binding of [3H]ligand to the subsequently washed preparations and this phenomenon was quite t...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/0024-3205(86)90428-5
更新日期：1986-04-07 00:00:00
abstract：:The effects of thioperamide, an H3 antagonist, and histamine H1 and H2 antagonists (s.c.) on morphine (s.c. or i.c.v.)- and U-50,488H (i.c.v.)-induced antinociception in male ddY mice were examined using the hot-plate (55 degrees C) test. Thioperamide significantly inhibited morphine-induced antinociception, but not U...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/0024-3205(94)00589-3
更新日期：1994-01-01 00:00:00
abstract：:The hypoglycemic and hypolipidemic effects of Lycium barbarum fruit water decoction, crude polysaccharide extracts (crude LBP), and purified polysaccharide fractions (LBP-X) in alloxan-induced diabetic or hyperlipidemic rabbits were investigated through designed sequential trials and by measuring blood glucose and ser...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/j.lfs.2004.04.056
更新日期：2004-11-26 00:00:00
abstract：AIMS:Studies on diabetes mellitus have shown that the phosphoinositide 3-kinase (PI3K)/serine threonine kinase (AKT)/forkhead box protein O1 (FoxO1) signaling pathway can regulate insulin secretion by modulating the expression of pancreatic and duodenal homeobox-1 (PDX-1). Therefore, it was hypothesized that the pathwa...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/j.lfs.2020.118145
更新日期：2020-10-01 00:00:00
abstract：:This study investigated the effect of berberine (BER) in the mouse forced swim test (FST) and in the tail suspension test (TST), two models predictive of antidepressant activity. We also investigated the antidepressant-like mechanism of BER by the combination of the desipramine [DES, an inhibitor of reuptake of noradr...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/j.lfs.2007.08.003
更新日期：2007-08-23 00:00:00
abstract：:In frog brain membranes, equilibrium saturation binding of the opiate agonist: 3H-etorphine and of the opiate antagonist: 3H-diprenorphine is biphasic. In the presence of sodium ions (120 mM), "kinetic conversions" (unchanged Bmax) are observed: high affinity binding of the 3H-agonist is decreased while high affinity ...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/0024-3205(83)90477-0
更新日期：1983-01-01 00:00:00
abstract：:The effects of the d- and l-isomers of pentazocine were compared to that of racemic pentazocine on contractions of the mouse isolated vas deferens. L-pentazocine inhibited electrically evoked contractions of the mouse vas deferens (MVD) in a dose-dependent manner (ID50 0.37 +/- 0.04 microM). In contrast, d-pentazocine...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/0024-3205(86)90446-7
更新日期：1986-07-14 00:00:00
abstract：:Significant elevation in plasma corticosterone of rats achieved by intraperitoneal (i.p.) administration of corticosterone (2.4 mg/kg) was associated with a rapid (2.5 min) and significant increase in hypothalamic histamine (HA) levels which persisted for 60 min. Midbrain and cortical HA concentrations were not affect...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/0024-3205(83)90115-7
更新日期：1983-03-07 00:00:00
abstract：:The effects of elevated glucose and aldose reductase inhibitor (ARI:ONO-2235) on nitric oxide (NO) production in cultured human umbilical endothelial cells (HUVEC) were evaluated. Aldose reductase and nitric oxide synthase(NOS) share NADPH as an obligate cofactor, therefore it is suggested that the enhanced of glucose...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/s0024-3205(96)00622-4
更新日期：1997-01-01 00:00:00
abstract：:The plasma concentration of the dopamine (DA) metabolite, homovanillic acid (HVA), is used as an indicator of central nervous system dopaminergic activity. Using percutaneously inserted catheters we were able to obtain blood samples simultaneously from the right and left internal jugular veins. Veno-arterial HVA plasm...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/0024-3205(91)90593-z
更新日期：1991-01-01 00:00:00
abstract：:The presence of neuropeptide tyrosine (NPY) in the intermediate lobe of the frog pituitary was demonstrated using indirect immunofluorescence, the immunogold technique and a specific radioimmunoassay combined with high pressure liquid chromatography (HPLC). A high density of NPY-containing fibers, was found among the ...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/0024-3205(86)90350-4
更新日期：1986-09-29 00:00:00
abstract：:Aim Doxorubicin (Dox) is one of the most cardiotoxic anti-cancerous drug that is widely used for broad-range of cancers. There is an urgent need for developing cardio-oncological therapeutic interventions. Natural products having both anti-cancerous potential as well as cardioprotective effects may hold a great potent...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/j.lfs.2018.05.021
更新日期：2018-07-15 00:00:00
abstract：:The level of hepatic lipidperoxides in chick embryos was determined during cataract formation resulting from glucocorticoid treatment. When 15 day old chick embryos were administered 0.25 mumol of hydrocortisone acetate their hepatic lipidperoxide level, determined by thio-barbituric acid, increased after a lag time o...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/0024-3205(84)90664-7
更新日期：1984-08-27 00:00:00
abstract：AIM:Exosomes, the nano-units (<200 nm), released from diverse cell types in the extracellular body fluid, possess non-immunogenic property and ability to cross the blood-brain barrier (BBB). Since exosomes carry biological information from their cells of origin, we hypothesize that priming cells with potential therapeu...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/j.lfs.2014.04.018
更新日期：2014-06-27 00:00:00
abstract：:The effects of free fatty acids in the blood on osmotic fragility and water content of erythrocytes when combined with hyperthermia were investigated. The isotonic buffer, containing the fatty acid, was added to the erythrocyte suspension to a final concentration of 200 microM/L. The samples were kept for one hour in ...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/s0024-3205(97)01184-3
更新日期：1998-01-01 00:00:00
abstract：AIMS:Tetramethylpyrazine (TMP), one of the active ingredients isolated from a Chinese herbal prescription, possesses protective effects against oxidative stress caused by high glucose in endothelial cells. In this study, the role of TMP in preventing muscle cells from palmitate-induced oxidative damage was investigated...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/j.lfs.2011.02.025
更新日期：2011-04-25 00:00:00
abstract：:The effect of chronic cigarette smoke exposure on the activity of rat trachea and lung ornithine decarboxylase (ODC) and s-adenosyl-methionine decarboxylase (AdoMet-DC) was determined. Male rats were exposed once daily for 10 min to mainstream (MS) or different sidestream (SS) dilutions of fresh Kentucky 2R1 cigarette...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/0024-3205(85)90567-3
更新日期：1985-12-09 00:00:00
abstract：:We investigated the effects of genistein and genistin on proliferation and apoptosis of human ovarian SK-OV-3 cells and explored the mechanism for these effects. SK-OV-3 cells were treated with genistein and genistin at various concentrations (ranging from 1 to 100 muM) either alone or in combination for 24 and 48 h. ...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/j.lfs.2006.12.031
更新日期：2007-03-20 00:00:00
abstract：:To clarify the mechanism of meconium-induced cellular injury, we examined the effect of meconium on the alveolar macrophages (AM) and bronchial epithelial cells (AK-D). Meconium obtained from healthy newborns was added to culture medium of AM and/or AK-D, which were cultured for 1 hour. Superoxide anion production of ...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/0024-3205(94)90026-4
更新日期：1994-01-01 00:00:00
abstract：:Obesity leads to various changes in the body. Among them, the existing inflammatory process may lead to an increase in the production of reactive oxygen species (ROS) and cause oxidative stress. Oxidative stress, in turn, can trigger mitochondrial changes, which is called mitochondrial dysfunction. Moreover, excess nu...
journal_title：Life sciences
pub_type： 杂志文章,评审
doi：10.1016/j.lfs.2017.11.019
更新日期：2018-01-01 00:00:00
abstract：:We studied the incorporation of 14C-formate into uric acid and allantoin in different organs (liver, lung, kidney, spleen), isolated hepatocytes, perfused liver and urine of the rat. Allantoin had a higher specific radioactivity than uric acid after 14C-formate load in the liver in vivo. This was found to be a strictl...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/0024-3205(96)00387-6
更新日期：1996-01-01 00:00:00
abstract：:The effect of gonadal steroids on the response to analgesia testing was determined in castrated male and female rats and castrated male and female rats treated with testosterone propionate (TP) and estradiol benzoate (EB), respectively. The time to respond to a noxious somatic stimulus in the form of heat was assessed...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/0024-3205(89)90631-0
更新日期：1989-01-01 00:00:00
abstract：:Stability and distribution of orally administered epidermal growth factor (EGF) were examined in newborn and 5-day-old pigs. Forty-five minutes after oral administration of iodine-125 labeled EGF, 60 and 50% of the radioactivity administered were recovered from the internal organs in newborn and 5-day-old pigs, respec...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/s0024-3205(98)00337-3
更新日期：1998-01-01 00:00:00
abstract：:The potential for a drug interaction between cyclosporin A and phenytoin was investigated in rats. Rats were treated daily for 14 days with cyclosporin A (50 mg/Kg, s.c.) and on the day of the experiment phenytoin (10 mg/Kg) was administered intravenously. The mean residence time, the elimination half-life and the vol...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/0024-3205(93)90140-x
更新日期：1993-01-01 00:00:00
abstract：:Dopamine (DA), one of the major sources of reactive oxygen species (ROS), is implicated in neuronal death associated with Parkinson's disease (PD). Preconditioning with oxidative stress has been shown to provide cytoprotection similar to ischemic preconditioning (IPC), against cell apoptosis. In this study, using the ...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/j.lfs.2005.04.048
更新日期：2005-11-19 00:00:00
