Abstract:
:We investigated the effects of genistein and genistin on proliferation and apoptosis of human ovarian SK-OV-3 cells and explored the mechanism for these effects. SK-OV-3 cells were treated with genistein and genistin at various concentrations (ranging from 1 to 100 muM) either alone or in combination for 24 and 48 h. Cell proliferation was estimated using an MTT assay, and cell cycle arrest was evaluated using FACS. Caspase-3 activity and annexin-based cell cycle analysis were used as measures of apoptosis. In addition, genistein- and genistin-induced cytotoxicity was determined by measuring release of LDH. Genistein treatment for 24 or 48 h substantially inhibited SK-OV-3 cell proliferation in a dose-dependent manner, and genistin treatment for 48 h also inhibited cell proliferation. Genistein caused cell cycle arrest at G2/M phase in dose- and time-dependent manner, and genistin caused cell cycle arrest not only at G2/M phase but also at G1 phase. Genistein markedly induced apoptosis and significantly increased LDH release, whereas genistin did not affect LDH release. Moreover, exposure to both genistein and genistin in combination for 48 h induced apoptosis without increasing LDH release. Genistein and genistin inhibit cell proliferation by disrupting the cell cycle, which is strongly associated with the arrest induction of either G1 or G2/M phase and may induce apoptosis. Based on our findings, we speculate that both genistein and genistin may prove useful as anticancer drugs and that the combination of genistein and genistin may have further anticancer activity.
journal_name
Life Scijournal_title
Life sciencesauthors
Choi EJ,Kim T,Lee MSdoi
10.1016/j.lfs.2006.12.031subject
Has Abstractpub_date
2007-03-20 00:00:00pages
1403-8issue
15eissn
0024-3205issn
1879-0631pii
S0024-3205(07)00048-3journal_volume
80pub_type
杂志文章相关文献
LIFE SCIENCES文献大全abstract:AIMS:The molecular pathogenesis of COVID-19 is similar to other coronavirus (CoV) infections viz. severe acute respiratory syndrome (SARS) in human. Due to scarcity of the suitable treatment strategy, the present study was undertaken to explore host protein(s) targeted by potent repurposed drug(s) in COVID-19. MATERIA...
journal_title:Life sciences
pub_type: 杂志文章,meta分析
doi:10.1016/j.lfs.2020.118096
更新日期:2020-09-15 00:00:00
abstract::The recently discovered highly specific delta opioid antagonist H-Tyr-Tic-Phe-Phe-OH (TIPP) was radiolabelled by catalytic tritiation of its precursor [Tyr (3', 5'-I2)1]TIPP. The tritiated ligand labelled rat brain membrane binding sites with a Kd value of 0.64 nM and a Bmax of 82 fmol/mg protein. Binding competition ...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/0024-3205(93)90649-n
更新日期:1993-01-01 00:00:00
abstract::Nitric oxide (NO) may be an intercellular modulator within the central nervous system. L-arginine, which results in NO synthesis, increased food intake in mice while the inhibitor of NO synthesis, L-NG-nitro arginine (L-NO Arg) inhibited food intake in food deprived mice. L-arginine, but not D-arginine, partially reve...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/0024-3205(91)90102-h
更新日期:1991-01-01 00:00:00
abstract:AIMS:This study evaluates ocular (iris, ciliary body and ciliary process) and nonocular (atria and lung) beta-adrenoceptors in rabbit to characterize the plasma membrane beta-adrenoceptors and binding affinities of beta-adrenoceptor antagonists. MAIN METHODS:The tissue segment binding method with a hydrophilic radioli...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/j.lfs.2008.11.016
更新日期:2009-01-30 00:00:00
abstract::Studies have adequately demonstrated the importance of maternal nutrition, particularly, micronutrients (folic acid, vitamin B12) and long chain polyunsaturated fatty acids (LCPUFAs) in determining pregnancy outcome. Reports indicate that children born preterm or to mothers with preeclampsia are at increased risk of d...
journal_title:Life sciences
pub_type: 杂志文章,评审
doi:10.1016/j.lfs.2014.09.026
更新日期:2015-05-15 00:00:00
abstract::This investigation examines the role of Angiotensin II in renal hemodynamic functions during acute unilateral ureteral obstruction (UUO) in a dog model. An electro magnetic flow probe was utilized to assess renal blood flow while the arteriovenous extraction technique of technetium 99m DTPA was utilized for the assess...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/0024-3205(88)90495-x
更新日期:1988-01-01 00:00:00
abstract::The purpose of this study was to examine whether the adhesion of polymorphonuclear leukocytes (PMNs) to endothelial cells and/or reactive oxygen species (ROS) released from PMNs are responsible for inducing angiogenesis. Angiogenesis was assessed by tube formation using endothelial cells obtained from bovine thoracic ...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/s0024-3205(00)00537-3
更新日期:2000-04-14 00:00:00
abstract:AIMS:The purpose of this study was to determine a relatively short-term effect of feeding an α-linolenic acid (ALA, n-3)-restricted, linoleic acid (LA, n-6)-adequate diet on neurotrophin contents and protein kinase activities in brain regions of the mouse. MAIN METHODS:After feeding mice a safflower oil (SAF) diet (AL...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/j.lfs.2010.09.003
更新日期:2010-10-09 00:00:00
abstract:AIMS:To investigate the effects of aerobic exercise training on cardiomyocyte ultrastructure, oxidative stress, and activation of protein synthesis pathways in a model of cardiomyopathy induced by doxorubicin (Dox). MAIN METHODS:Male Sprague Dawley rats were randomly assigned to Control (saline, sedentary), Dox/sedent...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/j.lfs.2020.118698
更新日期:2021-01-01 00:00:00
abstract::A microiontophoretic study using rats anesthetized with chloral hydrate and immobilized with gallamine triethiodide was carried out to compare the effect of talipexole (B-HT 920 CL2:2-amino-6-allyl-5,6,7,8-tetrahydro-4H-thiazolo [4,5-d]-azepine-dihydrochloride), a dopamine autoreceptor agonist, on dopaminergic neurons...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/0024-3205(91)90071-i
更新日期:1991-01-01 00:00:00
abstract::The use of more than one drug to achieve a desired effect has been a common practice in pharmacologic testing and in clinical practice. For example, combinations of analgesics are frequently prescribed with a view to enhancing pain relief and reducing adverse effects. It is also well established that administration of...
journal_title:Life sciences
pub_type: 杂志文章,评审
doi:10.1016/0024-3205(89)90148-3
更新日期:1989-01-01 00:00:00
abstract::Clinical studies of cholinergic pharmacotherapy, together with the putative role of the muscarinic receptor system in the neurophysiology of human behavior, support a possible muscarinic cholinergic involvement in schizophrenia. The present study has measured the density of [3H]AF-DX 384 labelled receptors (muscarinic...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/s0024-3205(99)00114-9
更新日期:1999-01-01 00:00:00
abstract::The relative efficiencies of microdialysis probes were determined both in vitro and in vivo using tritiated water. Tritiated water (THO) freely distributes throughout the fluid spaces of an experimental animal and, at equilibrium, the brain extracellular concentration of THO is the same as the plasma concentration. Mi...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/0024-3205(88)90063-x
更新日期:1988-01-01 00:00:00
abstract::The effects of various vasorelaxant agents on aortas from control and genetically diabetic rats were examined. The concentration-response curves for the isoproterenol (ISO)-induced relaxation of both aortic strips with and without endothelium are shifted to the right in diabetic rats. The relaxation responses of diabe...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/0024-3205(92)90287-y
更新日期:1992-01-01 00:00:00
abstract::The purpose of this study was to determine the effects of the 7288ctc Morris hepatoma on heart size and performance. Hearts from tumor bearing and control animals were perfused in the working configuration one to three weeks post implantation. As tumor growth progressed there was an inverse linear relationship between...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/0024-3205(94)00819-1
更新日期:1994-01-01 00:00:00
abstract::An increase in natural killer (NK) cell activity can be conditioned with a one trial learning paradigm to demonstrate the interaction between the central nervous system (CNS) and the immune system. In order to demonstrate learning possibilities during 'non-conscious' state, mice were anesthetized with a ketamin/rompun...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/0024-3205(92)90573-8
更新日期:1992-01-01 00:00:00
abstract::The present study investigated the effects of nitric oxide synthase (NOS) inhibitors on the seizure threshold of DMCM in mice. The seizure threshold of DMCM was evaluated using an intravenous infusion technique. The threshold of DMCM was significantly decreased by pretreatment with N-nitro-L-arginine (NOARG; 8 mg/kg) ...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/s0024-3205(97)00226-9
更新日期:1997-01-01 00:00:00
abstract:AIMS:3-Nitropropionic acid (3-NPA) is a naturally occurring fungal toxin that leads to ATP-depletion by inhibiting mitochondrial succinate dehydrogenase and produces chemical anoxia. The present study was conducted to identify the involvement of inhibitory system in 3-NPA-induced depression of spinal reflexes. METHODS...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/j.lfs.2008.09.017
更新日期:2008-11-21 00:00:00
abstract::Several different vasodilator substances can be released by vascular endothelium in response to mechanical stimuli and vasoactive agents. The purpose of this study was to determine whether there is a male-female difference in the relative contributions of nitric oxide (NO) and endothelium-derived hyperpolarizing facto...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/s0024-3205(02)01851-9
更新日期:2002-08-23 00:00:00
abstract::We have investigated the possible involvement of arachidonic acid metabolites in dopamine-induced inhibition of adrenocortical steroidogenesis. Administration of dopamine (5 x 10(-5) M) for 20 min to perifused frog adrenal slices caused a marked reduction of the release of both prostaglandin E2 (PGE2) and 6-keto-PGF1 ...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/0024-3205(89)90292-0
更新日期:1989-01-01 00:00:00
abstract::Hyaluronan (HA) is a multifunctional high molecular weight polysaccharide found throughout the animal kingdom, especially in the extracellular matrix (ECM) of soft connective tissues. HA is thought to participate in many biological processes, and its level is markedly elevated during embryogenesis, cell migration, wou...
journal_title:Life sciences
pub_type: 杂志文章,评审
doi:10.1016/j.lfs.2007.02.037
更新日期:2007-05-01 00:00:00
abstract::Single unit recordings were obtained from putative dopaminergic neurons in the substantia nigra of awake, freely moving rats. The cells exhibited waveforms, range of firing rates and types of firing patterns identical to those of identified DA neurons of anesthetized or paralyzed rats. Two firing patterns were observe...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/0024-3205(85)90448-5
更新日期:1985-05-20 00:00:00
abstract::Quercetin, a plant flavonoid, blocks signal transduction pathways by inhibiting 1-phosphatidylinositol 4-kinase (EC 2.7.1.67, PI kinase) and 1-phosphatidylinositol 4-phosphate 5-kinase (EC 2.7.1.68, PIP kinase), resulting in a reduction of inositol 1,4,5-trisphosphate (IP3) concentration which decreases the release of...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/0024-3205(95)02085-w
更新日期:1995-01-01 00:00:00
abstract::We found that platelet-activating factor (PAF) stimulated the production of prostaglandin (PG) E2 in MC3T3-E1 cells in a time- and dose-dependent manner. 1.0 microM PAF gave a maximal stimulation of PGE2 production by MC3T3-E1 cells after a 4 hr PAF-treatment. Furthermore, the PAF-induced PGE2 production was abolished...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/0024-3205(91)90598-6
更新日期:1991-01-01 00:00:00
abstract::In the present study the formation of thrombin-antithrombin-complexes (TAT) during incubation of thrombin (0.89, 4.5, 8.9 nmol/l) and antithrombin (4.6 micromol/l) on the surface of cultured human EC, derived from different parts of the circulation, and on the surface of human vessel segments was studied. In the absen...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/s0024-3205(98)00416-0
更新日期:1998-01-01 00:00:00
abstract::Definition of the physiological and pharmacological properties of primary afferent neurons by the use of capsaicin and its analogues (e.g. resiniferatoxin) has represented one of the most active areas of research of the last decade (1-4 for reviews). In the past 3 years many important advancements have been made in th...
journal_title:Life sciences
pub_type: 杂志文章,评审
doi:10.1016/0024-3205(91)90169-c
更新日期:1991-01-01 00:00:00
abstract::Mitochondria play a crucial role in the induction and execution of apoptosis. Accordingly, recent suggestions have been made to use agents that directly act on mitochondria to trigger apoptosis so that drug-sensitive and-resistant tumour cells can be eliminated. To test this hypothesis, human hepatocarcinoma HepG2 and...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/s0024-3205(02)02103-3
更新日期:2002-10-25 00:00:00
abstract::The purpose of this research is to elucidate the pharmacological mechanism mediating vesicating effects of sulfur mustard (HD) and identify an antidote to its action. HD causes blisters because epithelial cells lose their attachments. Epithelial cell adhesion is under control of the local cytotransmitter acetylcholine...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/s0024-3205(03)00074-2
更新日期:2003-03-28 00:00:00
abstract::Relationship between in vivo down-regulation of cardiac muscarinic receptors and changes in their encoding mRNA was investigated. Rats were treated either once or for ten days with an irreversible inhibitor of acetylcholinesterase, followed by measurements of cardiac acetylcholinesterase, the density and affinity of m...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/0024-3205(91)90615-i
更新日期:1991-01-01 00:00:00
abstract::The growth response of aortic vascular smooth muscle cells (VSMCs) to chronic hypertension includes vascular hypertrophy. We have shown previously that angiotensin II positively regulates the expression of the human vascular smooth muscle (SM) alpha-actin gene. To further expand our understanding of vasoactive peptide...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/0024-3205(96)00332-3
更新日期:1996-01-01 00:00:00