Abstract：
:Relationship between in vivo down-regulation of cardiac muscarinic receptors and changes in their encoding mRNA was investigated. Rats were treated either once or for ten days with an irreversible inhibitor of acetylcholinesterase, followed by measurements of cardiac acetylcholinesterase, the density and affinity of muscarinic receptors, and the concentration of mRNA coding for these receptors. mRNA was quantitated using the sensitive method of DNA-excess solution hybridization. Our data indicate that while short-term treatment resulted in a marked decrease in the density of cardiac muscarinic receptors by 34%, there was no accompanying significant change in the concentration of their mRNA. In contrast, long-term inhibition of acetylcholinesterase significantly decreased the concentration of both receptors and mRNA by 40% and 29%, respectively. These results are indicative of multiple mechanisms of down-regulation of cardiac muscarinic receptors, some of which might involve alterations at the transcriptional level.
journal_name
Life Scijournal_title
Life sciencesauthors
Zhu SZ,Wang SZ,Abdallah EA,el-Fakahany EEdoi
10.1016/0024-3205(91)90615-isubject
Has Abstractpub_date
1991-01-01 00:00:00pages
2579-84issue
26eissn
0024-3205issn
1879-0631pii
0024-3205(91)90615-Ijournal_volume
48pub_type
杂志文章相关文献
LIFE SCIENCES文献大全abstract：:Tobacco smoke exposure has been shown to inhibit brain and platelet MAO B in animals and in humans. Though the mechanism(s) responsible for MAO B inhibition are not known, studies in rodents have shown that nicotine administration does not inhibit brain MAO B. In this study we investigated whether brain MAO B is also ...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/s0024-3205(98)00251-3
更新日期：1998-01-01 00:00:00
abstract：:Low pH medium has been shown to activate the 'efferent' function of capsaicin-sensitive primary sensory neurons. Calcitonin gene-related peptide (CGRP) is released from capsaicin-sensitive afferents of guinea-pig superior sagittal and transverse sinuses (SSTS), by capsaicin or bradykinin. Here, we report that low pH m...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/0024-3205(91)90263-b
更新日期：1991-01-01 00:00:00
abstract：:Mucosal mast cells are implicated in visceral hypersensitivity associated with irritable bowel syndrome (IBS). In this study, we investigated the role of mast cells in the development of visceral hypersensitivity by using mast cell deficient (Ws/Ws) rats and their control (W+/W+). In W+/W+ rats, an injection of 2,4,6-...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/j.lfs.2007.11.027
更新日期：2008-02-13 00:00:00
abstract：AIMS:To investigate the effects of high doses of pentazocine on antinociception induced by a high dose of morphine and the role of the kappa-opioid receptors (KORs) in these effects in mice. MAIN METHODS:Sixty-six C57BL/6J mice were pretreated with a KOR antagonist, nor-binaltorphimine (nor-BNI) (10mg·kg(-1)), or a no...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/j.lfs.2015.02.023
更新日期：2015-06-01 00:00:00
abstract：:The effects of exposure to a schedule of unpredictable cold-immobilization stress on voluntary ethanol consumption were examined. Following testing for ethanol preference, rats were divided into high, medium and low ethanol consuming groups on the basis of daily ethanol intake (g/kg/day) and exposed to immobilization ...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/0024-3205(87)90580-7
更新日期：1987-03-30 00:00:00
abstract：:As a step in the purification and characterization of the glucose transporter from rat skeletal muscle, we have reconstituted glucose transport activity in liposomes. Plasma membranes were prepared from skeletal muscle which display D-glucose reversible binding of cytochalasin B (10 pmol sites/mg protein; KD = 0.3 mic...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/0024-3205(87)90503-0
更新日期：1987-06-15 00:00:00
abstract：:Visual evoked responses (VER) to counterphased gratings were recorded from area 17 of cat visual cortex prior to and following administration of diisopropylfluorophosphate (DFP). The VER and acetylcholinesterase (AChE) activity of blood, retina, and visual cortex were reduced significantly following DFP administration...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/0024-3205(87)90282-7
更新日期：1987-12-14 00:00:00
abstract：AIMS:Long non-coding RNAs (LncRNAs) play central roles in the formation and development of gastric cancer (GC). The aim of this study was to evaluate the expression of PURPL and NONHSAT062994 and the relationship between their expressions with clinical characteristics in GC. MAIN METHODS:PURPL and NONHSAT062994 LncRNA...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/j.lfs.2019.116904
更新日期：2019-11-15 00:00:00
abstract：AIMS:To determine whether ginsenoside Rg1 is involved in scratch wound healing through altered expression of related molecules in astrocytes and improved functional recovery after spinal cord injury (SCI). MATERIALS AND METHODS:Astrocytes were isolated from rats, followed by Rg1 treatment. The wound healing test was p...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/j.lfs.2020.117642
更新日期：2020-07-01 00:00:00
abstract：:The effects of denervation of alpha 2 adrenoceptor binding sites were examined in canine arteries and veins. Denervation of the lower abdominal aorta, renal and femoral arteries and femoral veins marked reduced vessel norepinephrine concentrations. Denervation had little effect on the concentration of alpha 2 adrenoce...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/s0024-3205(83)80005-8
更新日期：1983-07-25 00:00:00
abstract：AIMS:To investigate how Placental Mesenchymal Stem Cells (P-MSCs) would adapt themselves and survive under hypoxic conditions which are prevalent in most injury sites. MAIN METHODS:P-MSCs were isolated from term placenta and characterised under normoxia and hypoxia (2-2.5% O2). Cells were examined for morphology and s...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/j.lfs.2017.06.016
更新日期：2017-08-01 00:00:00
abstract：:The effects of naloxone or thyrotropin releasing hormone (TRH) upon neurologic outcome were evaluated in gerbil models of cerebral ischemia. Following temporary bilateral carotid occlusion, hypotension was transiently reversed by these endorphin antagonists. However, neither drug altered time to awaken, time to death,...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/0024-3205(82)90419-2
更新日期：1982-07-26 00:00:00
abstract：:Of primary interest for every enzyme is the identification of its physiological substrates. However, the vast structural diversity of endogenous metabolites, coupled with the overlapping activities of numerous enzymes, makes it difficult to deduce the identity of natural substrates for a given enzyme based on in vitro...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/j.lfs.2005.05.019
更新日期：2005-08-19 00:00:00
abstract：AIMS:Aging and drug-induced side effects may contribute to deteriorate mitochondrial bioenergetics in many tissues, including kidney and liver. One possibility is that the combination of both aging and drug toxicity accelerates the process of mitochondrial degradation, leading to progressive bioenergetic disruption. We...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/j.lfs.2013.07.004
更新日期：2013-09-03 00:00:00
abstract：:The effects of central administration of two serotonin receptor agonists, 8-OH-DPAT and mCPP, on tuberoinfundibular dopaminergic (TIDA) neuron activity and serum prolactin (PRL) levels in ovariectomized, estrogen-treated rats were determined. 8-OH-DPAT dose-dependently (0.1-10 microgram/rat, icv) stimulated serum PRL ...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/0024-3205(96)00077-x
更新日期：1996-01-01 00:00:00
abstract：:An increase in the weight of the thyroid gland, accompanied by reductions in uptake and release of 1311, serum T3 and reverse T3 concentrations and an increase in serum TSH concentration, occurs during the development of renal hypertension in rats. Renal function, as assessed by BUN, serum and urinary creatinine conce...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/0024-3205(82)90274-0
更新日期：1982-02-15 00:00:00
abstract：:To investigate the properties of the gamma-aminobutyric acid (GABA) synthesizing enzyme, glutamate decarboxylase (GAD), in the brain and the pancreatic islets of the rat, GABA concentration in the brain and the pancreatic islets was measured after administration of 3-mercaptopropionic acid (3-MP) at 25 mg/kg intraperi...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/0024-3205(94)00445-5
更新日期：1994-01-01 00:00:00
abstract：AIMS:Transient ischemia of osteoporotic bones during elective orthopedic surgery or fracture repair carries risks for serious complications, and estrogen loss or replacement has a potential to influence ischemia-reperfusion-induced inflammatory activation. To clarify this, we investigated the periosteal inflammatory ch...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/j.lfs.2010.11.004
更新日期：2011-01-17 00:00:00
abstract：:The purpose of this study is to report the development of a non-radioactive fluorescent peptide assay for measuring protein kinase C activity (PKC). The assay is based on a glycogen synthase derived fluorescent peptide that is phosphorylated by PKC. Phosphorylation causes the peptide to migrate toward the anode while ...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/0024-3205(95)02149-d
更新日期：1995-01-01 00:00:00
abstract：:The platelet activating factor (PAF) receptor of the guinea pig peritoneal eosinophil was characterized by radioligand ([3H]WEB 2086) binding and measurement of PAF-stimulated membrane GTPase activity. Specific binding of [3H]WEB 2086 was rapid, reversible and saturable, with an equilibrium KD of 20.4 nM at 0 degrees ...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/0024-3205(93)90045-5
更新日期：1993-01-01 00:00:00
abstract：AIMS:The p53 protein, also called guardian of the genome, has a key role in cell cycle regulation. It is activated under stressful circumstances, such as DNA damage which results in permanent arrest or cell death. The protein is disabled in several types of human cancer due to over-expression of the two regulators, Mdm...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/j.lfs.2015.12.047
更新日期：2016-01-15 00:00:00
abstract：AIMS:We recently reported that acute exposure to nicotine vasodilates the renal vasculature of male rats via facilitation of endothelial nitric oxide synthase (eNOS). In this study, we investigated whether this effect of nicotine is sexually dimorphic and the role of estrogen in modulating the nicotine effect. MAIN ME...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/j.lfs.2010.11.009
更新日期：2011-01-17 00:00:00
abstract：:We measured the concentration of immunoreactive (IR) hEGF in various body fluids by radioimmunoassay (RIA) and evaluated its size heterogeneity by size exclusion high performance liquid chromatography combined with RIA or with time-resolved immunofluorometric assay (TR-IFMA). Mean concentration was 80 ng/ml in urine, ...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/0024-3205(87)90069-5
更新日期：1987-06-29 00:00:00
abstract：:Systemic administration of the selective D1 antagonist, SCH 23390, caused significant motor changes in healthy African green monkeys. The effects included the parkinsonian signs of motor freezing, incoordination, bradykinesia, poverty of movement, tremor and depressed blink rate. SCH 23390 administered to MPTP-treated...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/0024-3205(91)90299-q
更新日期：1991-01-01 00:00:00
abstract：AIMS:Oxidative stress is involved in cholestasis-induced hepatic damage. Therefore, antioxidant therapy is a recommended therapeutic strategy. Studies have illustrated that chromium can enhance antioxidative capacity leading to a resolution of oxidative stress. The aim of this study was to assess whether chromium has p...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/j.lfs.2009.02.003
更新日期：2009-04-24 00:00:00
abstract：:The validity of a photoelectric method for continuous cerebral blood volume (CBV) measurement was tested and modified for the rat's brain. A new way of introducing a miniature light source between the two hemispheres and fixing a light sensitive silicone blue cell to the outer surface of the parietal bone was develope...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/0024-3205(86)90163-3
更新日期：1986-11-03 00:00:00
abstract：:The development of the specific uptake of dopamine in the rat striatum during the early postnatal period is compared with the ontogenetic changes of the specific binding of (3H)GBR 12783 to the site of uptake inhibition. During maturation, the increase in the specific binding of (3H)GBR 12783 parallels the increase in...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/0024-3205(89)90563-8
更新日期：1989-01-01 00:00:00
abstract：AIMS:The renin-angiotensin system (RAS) plays a major role in cardiovascular diseases in postmenopausal women, but little is known about its importance to lower urinary tract symptoms. In this study we have used the model of ovariectomized (OVX) estrogen-deficient rats to investigate the role of RAS in functional and m...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/j.lfs.2013.09.008
更新日期：2013-11-19 00:00:00
abstract：:Vascular disease is one of the complicating features of diabetes mellitus. Magnesium deficiency has recently been proposed as a novel factor implicated in the pathogenesis of diabetes complications. Several studies have indicated that hypertension in diabetic patients is an independent altered reaction of blood vessel...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/j.lfs.2004.07.027
更新日期：2005-02-11 00:00:00
abstract：:Using a two-lever drug discrimination procedure, six rats were trained to discriminate 0.5 mg/kg of racemic 1-(2,5-dimethoxy-4-iodophenyl)-2-aminopropane (DOI) from saline. Once trained, the animals demonstrated a dose-related decrease in discriminative performance upon administration of lower doses of DOI (ED50 = 0.1...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/0024-3205(86)90461-3
更新日期：1986-09-01 00:00:00
