A non-radioactive fluorescent method for measuring protein kinase C activity.

abstract：:A response in heat shock protein 70 (hsp 70) expression in the beta-naphthoflavone (BNF) treated rainbow trout (Oncorhynchus mykiss) corresponded to altered metabolic status of the liver as evidenced by the lower phosphoenolpyruvate carboxykinase (PEPCK), lactate dehydrogenase and 3-hydroxyacylcoA dehydrogenase activi...

journal_title：Life sciences

authors： Vijayan MM,Pereira C,Forsyth RB,Kennedy CJ,Iwama GK

更新日期：1997-01-01 00:00:00

abstract：:Rhesus monkeys were trained to discriminate injections of saline from those of beta-carboline-3-carboxylic acid ethyl ester (beta-CCE), a compound that binds to the benzodiazepine receptor, but often has actions opposite to those of the benzodiazepines. A benzodiazepine agonist midazolam and low doses of a specific be...

journal_title：Life sciences

authors： Takada K,Winger G,Cook J,Larscheid P,Woods JH

更新日期：1986-03-17 00:00:00

abstract：:The hyperphagia and rapid body weight gain normally observed in young obese (ob/ob) mice were abolished by removal of their adrenal glands, whereas food intake and weight gain of lean mice were not significantly affected by adrenalectomy. Adrenalectomy lowered body energy density (kcal/g carcass) in obese mice more th...

journal_title：Life sciences

authors： Vander Tuig JG,Ohshima K,Yoshida T,Romsos DR,Bray GA

更新日期：1984-04-09 00:00:00

abstract：AIMS:Cancer Stem Cells (CSCs) refers to heterogeneous tumor cells retaining the abilities of self-renewal and differentiation. This study used mRNAsi, which is an index to describe the similarity between tumor cells and CSCs, to define genes involved in endometrial carcinoma. MATERIALS AND METHODS:The mRNA expression ...

journal_title：Life sciences

authors： Liu J,Wu Z,Sun R,Nie S,Meng H,Zhong Y,Nie X,Cheng W

更新日期：2020-10-01 00:00:00

abstract：:A radioimmunoassay for orexin A has been developed. Anti-orexin A antiserum was raised in New Zealand white rabbits immunized with a conjugate of synthetic orexin A with bovine serum albumin. This antibody did not crossreact with orexin B, hypothalamic hormones, pituitary hormones, neuropeptides or gut hormones. Radio...

journal_title：Life sciences

authors： Mitsuma T,Hirooka Y,Kayama M,Mori Y,Yokoi Y,Rhue N,Ping J,Izumi M,Ikai R,Adachi K,Nogimori T

更新日期：2000-01-01 00:00:00

abstract：:Cd2+ (0.4 mg/kg) administration to growing rats (45 +/- 5 g) intraperitoneally, daily for 30 days was found to decrease the activity of superoxide dismutase (SOD) in all the brain regions, except hippocampus. The concentrations of lipid peroxides were significantly elevated in the cerebellum, cerebral cortex, corpus s...

journal_title：Life sciences

authors： Shukla GS,Hussain T,Chandra SV

更新日期：1987-11-09 00:00:00

abstract：:There are many studies of the mechanisms of antidepressants; however, most of these studies were conducted on the hippocampus or frontal cortex. In the present study, we hypothesized that the nucleus accumbens and caudate/putamen might be major targets for antidepressant effects. Thus, we focused on G(olf) protein, a ...

journal_title：Life sciences

authors： Taoka H,Hamamura T,Endo S,Miki M,Lee Y,Miyata S,Toma K,Ishihara T,Sagara H,Gomita Y,Kuroda S

更新日期：2006-06-27 00:00:00

abstract：:DL-alpha-difluoromethylornithine (DFMO), 4-deoxypyridoxine (4-DOP), and methylglyoxal bis (guanylhydrazone) (MGBG) were tested as inhibitors of acute skin graft rejection. Proximal full thickness tail skins were exchanged between C57BL/6 and Balb/C mice. Distal autografts were placed to monitor healing. Inhibitors wer...

journal_title：Life sciences

authors： Campbell RA,Kurtz GL,Norton-Reel DJ

更新日期：1991-01-01 00:00:00

abstract：:Accumulation of excess fluoride in the environment poses serious health risks to plants, animals, and humans. This endangers human health, affects organism growth and development, and negatively impacts the food chain, thereby affecting ecological balance. In recent years, numerous studies focused on the molecular mec...

journal_title：Life sciences

authors： Zuo H,Chen L,Kong M,Qiu L,Lü P,Wu P,Yang Y,Chen K

更新日期：2018-04-01 00:00:00

abstract：:Alpha-adrenergic receptors may play an important role in regulating vascular tone and reactivity. To study alpha-adrenergic receptors in blood vessels, we have developed a method to characterize and quantitate alpha-adrenergic receptors in a particulate fraction of individual rabbit aortas using the high specific acti...

journal_title：Life sciences

authors： Tsujimoto G,Bristow MR,Hoffman BB

更新日期：1984-02-13 00:00:00

abstract：:We contrast the effects of three intermediate electron acceptors (IEAs) on the highly quantitative ESTA bioassay for human growth hormone. This is a microculture tetrazolium assay based upon the in vitro reduction of the tetrazolium salt MTT, by Nb2 cells which have been activated with hGH. Each of the IEAs influenced...

journal_title：Life sciences

authors： Goodwin CJ,Holt SJ,Downes S,Marshall NJ

更新日期：1996-01-01 00:00:00

abstract：AIMS:The prognoses of patients with gastric cancer(GC) vary in different stages, which is mainly due to the great differences in tumor and tumor microenvironment. This study is aimed to explore the specific differences. MAIN METHODS:Based on RNAseq-based expression data from The Cancer Genome Atlas database and GSE154...

journal_title：Life sciences

authors： Wan L,Tan N,Zhang N,Xie X

更新日期：2020-11-15 00:00:00

abstract：:Previous research has shown that the CAMK (calcium/calmodulin dependent protein kinase) inhibitor, KN62, can lead to reductions in insulin stimulated glucose transport. Although controversial, an L-type calcium channel mechanism has also been hypothesized to be involved in insulin stimulated glucose transport. The pur...

journal_title：Life sciences

authors： Wright DC,Fick CA,Olesen JB,Lim K,Barnes BR,Craig BW

更新日期：2004-01-02 00:00:00

abstract：AIMS:Fenofibrate is a peroxisome proliferator-associated receptor alpha agonist (PPARα) used clinically for the management of dyslipidemia and is a myocardial fatty acid oxidation stimulator. It has also been shown to have cardiac anti-hypertrophic properties but the effects of fenofibrate on the development of eccentr...

journal_title：Life sciences

authors： Dhahri W,Couet J,Roussel É,Drolet MC,Arsenault M

更新日期：2013-01-17 00:00:00

abstract：AIMS:We examined the nature of pharmacological interaction after coadministration of melatonin with clonidine or neostigmine on formalin-induced nociception at the spinal level. Further, the role of melatonin receptor subtypes in melatonin-induced antinociception was clarified. MAIN METHODS:Catheters were inserted int...

journal_title：Life sciences

authors： Yoon MH,Park HC,Kim WM,Lee HG,Kim YO,Huang LJ

更新日期：2008-12-19 00:00:00

abstract：AIMS:Acute respiratory distress syndrome (ARDS) is characterized by acute hypoxemia with diffuse alveolar damage and increased pulmonary microvascular permeability. Honokiol (HKL), the principal active ingredient of Chinese herb magnolia officinalis, protected the lung of experimental ARDS models via attenuation of inf...

journal_title：Life sciences

authors： Chen L,Li W,Qi D,Lu L,Zhang Z,Wang D

更新日期：2018-10-01 00:00:00

abstract：AIMS:We have developed biochemical and cell based assays to characterize small therapeutic molecules that inhibit the DNA damage checkpoint enzyme, Chk1 (Checkpoint kinase 1). MAIN METHODS:To prepare a screen of large chemical libraries, we purified the full-length and the catalytic domain versions of human Chk1. We c...

journal_title：Life sciences

authors： Ferry G,Studeny A,Bossard C,Kubara PM,Zeyer D,Renaud JP,Casara P,de Nanteuil G,Wierzbicki M,Pfeiffer B,Prudhomme M,Leonce S,Pierré A,Boutin JA,Golsteyn RM

更新日期：2011-08-15 00:00:00

abstract：:We investigated lithium-induced changes in norepinephrine (NE) catabolism. NE and its major metabolites 3-methoxy-4-hydroxyphenylglycol (MHPG) and 3,4-dihydroxyphenyl glycol (DHPG), ions such as lithium (Li(+)), magnesium (Mg(2+)), and potassium (K(+)) were measured in rat plasma and cerebral cortex using an HPLC meth...

journal_title：Life sciences

authors： Sastre E,Nicolay A,Bruguerolle B,Portugal H

更新日期：2005-07-01 00:00:00

abstract：:Chronic administration of narcotic mu opioid agonists results in tolerance and dependence. We propose that agonist stimulation causes a gradual conversion of mu receptors to a constitutively active state (mu*) as a key step in tolerance and physical dependence. We provide evidence in support of the existence of mu* in...

journal_title：Life sciences

authors： Wang Z,Bilsky EJ,Porreca F,Sadée W

更新日期：1994-01-01 00:00:00

abstract：:A sensitive and specific radioimmunoassay for substance P has been developed and its full characterisation is described. The assay was used to investigate the stability of substance P in tissue in order to establish the optimal conditions of tissue storage for maximal recovery of the peptide. The results indicate unex...

journal_title：Life sciences

authors： McGregor GP,Bloom SR

更新日期：1983-02-07 00:00:00

abstract：:Leukocyte interactions with endothelial cell monolayers (ECM) and smooth muscle cells (SMC) play an important role during inflammatory processes. Several studies describe an inhibitory effect of dexamethasone on polymorphonuclear leukocytes (PMNL), endothelial cell function, and interleukin-1 (IL-1) release. Aim of th...

journal_title：Life sciences

authors： Hofbauer R,Gmeiner B,Handler S,Speiser W,Kapiotis S,Frass M

更新日期：1999-01-01 00:00:00

abstract：AIMS:The recent outbreak of pandemic severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2) has led the world towards a global health emergency. Currently, no proper medicine or effective treatment strategies are available; therefore, repurposing of FDA approved drugs may play an important role in overcoming the ...

journal_title：Life sciences

authors： Sharma K,Morla S,Goyal A,Kumar S

更新日期：2020-10-15 00:00:00

abstract：:Human alpha-fetoprotein (AFP) was expressed in Saccharomyces cerevisiae, with a plasmid containing the cDNA sequence for human AFP fused with the rat AFP signal peptide. The recombinant AFP was purified from the yeast lysate by DEAE-cellulose and immunoaffinity chromatography. The amino acid composition and the molecu...

journal_title：Life sciences

authors： Yamamoto R,Sakamoto T,Nishi S,Sakai M,Morinaga T,Tamaoki T

更新日期：1990-01-01 00:00:00

abstract：:Brain noradrenaline takes part in the regulation of several brain functions. The formation of brain noradrenaline depends on brain tyrosine (Tyr) levels, which associates with the ratio in plasma of Tyr to other large, neutral amino acids (LNAA). Tyr metabolism has been studied in users of the new generation combined ...

journal_title：Life sciences

authors： Møller SE,Maach-Møller B,Olesen M,Madsen B,Madsen P,Fjalland B

更新日期：1995-01-01 00:00:00

abstract：AIMS:Overproduction of nitric oxide (NO) from inducible NO synthase (iNOS) plays many pivotal roles in various inflammatory diseases. In this study, we examined the effects of 6 flavonoids on lipopolysaccharide (LPS)-induced NO generation in macrophage (Mφ), and addressed molecular mechanisms of cardamonin. MAIN METHO...

journal_title：Life sciences

authors： Takahashi A,Yamamoto N,Murakami A

更新日期：2011-08-29 00:00:00

abstract：AIMS:To determine whether ginsenoside Rg1 is involved in scratch wound healing through altered expression of related molecules in astrocytes and improved functional recovery after spinal cord injury (SCI). MATERIALS AND METHODS:Astrocytes were isolated from rats, followed by Rg1 treatment. The wound healing test was p...

journal_title：Life sciences

authors： Xu L,Tang YY,Ben XL,Cheng MH,Guo WX,Liu Y,Lu ZF,Deng JL

更新日期：2020-07-01 00:00:00

abstract：:The effects of pretreatment with selective mu-, delta- and kappa-opioid receptor antagonists on the enhancement of the pentobarbital-induced loss of the righting reflex by morphine were examined in mice. Mice injected with pentobarbital (50 mg/kg, i.p.) showed an increased duration for the loss of the righting reflex ...

journal_title：Life sciences

authors： Kamei J,Ohsawa M,Nagase H

更新日期：1996-01-01 00:00:00

abstract：:The reinforcing effects of cocaine and methylphenidate have been linked to their ability to block dopamine transporters (DAT). Though cocaine and methylphenidate have similar in vitro affinities for DAT the abuse of methylphenidate in humans is substantially lower than of cocaine. To test if differences in in vivo pot...

journal_title：Life sciences

authors： Volkow ND,Wang GJ,Fowler JS,Fischman M,Foltin R,Abumrad NN,Gatley SJ,Logan J,Wong C,Gifford A,Ding YS,Hitzemann R,Pappas N

更新日期：1999-01-01 00:00:00

abstract：AIM:Membranous nephropathy remains the most common form of the nephrotic syndrome in adults. The combination therapy of steroid and cyclophosphamide has routinely been used. Although satisfactory therapeutic efficacy can be achieved, its side effect on reproductive system has been a concern. Mizoribine is an imidazole ...

journal_title：Life sciences

authors： Wang X,Song X,Liu Y,Zhang W,An W,Tu Y

更新日期：2016-06-01 00:00:00

abstract：:Morphine sulfate and other opiate/narcotic analgesics are the most effective treatments for acute and chronic severe pain. However, their clinical utility is often hampered by the development of analgesic tolerance. This complex pathophysiological cycle contributes significantly to decreased quality of life in the gro...

journal_title：Life sciences

authors： Salvemini D,Neumann W

更新日期：2010-04-10 00:00:00