Abstract:
AIMS:To investigate how Placental Mesenchymal Stem Cells (P-MSCs) would adapt themselves and survive under hypoxic conditions which are prevalent in most injury sites. MAIN METHODS:P-MSCs were isolated from term placenta and characterised under normoxia and hypoxia (2-2.5% O2). Cells were examined for morphology and surface marker variations by flow cytometry analysis. Glucose stimulated insulin secretion was assayed by Insulin ELISA Kit. Gene expression levels were estimated using Real Time PCR for hypoxia inducible factor1 alpha, Insulin (INS), Glucose transporters (GLUT-1, GLUT-2 and GLUT-3), Adhesion Proteins- Integrins, Fibronectin1 (FN1), E-Cadherin (CDH1), and N-Cadherin (CDH2) and angiogenesis marker VEGFA. Immunofluorescence assay was done to confirm the presence of C-Peptide, GLUT 2, E-Cadherin and ITGB3. Adhesion was confirmed assessed on fibronectin binding. KEY FINDINGS:We show that insulin secretion is not hampered under hypoxia. We found an upregulation of glucose transporters under hypoxia indicating enhanced glucose uptake needed to cater to metabolic demands of proliferating cells. Up regulation of adhesion molecules was seen under hypoxia indicative of a favoured environment for retention of cells at the injury site. We also found increased level of angiogenesis of P-MSCs under hypoxia. SIGNIFICANCE:Our present study thus demonstrates for the first time that P-MSCs modulate themselves under hypoxic conditions by secreting insulin, up regulating glucose transporters and adhesion molecules and eventually exhibiting an increased angiogenic potential. We thus infer that priming P-MSCs under hypoxia, could make them more suitable for wound healing applications.
journal_name
Life Scijournal_title
Life sciencesauthors
Mathew SA,Chandravanshi B,Bhonde Rdoi
10.1016/j.lfs.2017.06.016subject
Has Abstractpub_date
2017-08-01 00:00:00pages
85-92eissn
0024-3205issn
1879-0631pii
S0024-3205(17)30302-8journal_volume
182pub_type
杂志文章相关文献
LIFE SCIENCES文献大全abstract::Muscarinic agonists elicit contraction in the standard guinea pig ileum bioassay through activation of M3 muscarinic receptors that are also linked to phosphoinositide hydrolysis. Surprisingly, the most abundant muscarinic receptor in the ileum is the M2 which causes a specific inhibition of cyclic AMP accumulation el...
journal_title:Life sciences
pub_type: 杂志文章,评审
doi:10.1016/0024-3205(94)00035-q
更新日期:1995-01-01 00:00:00
abstract::The actions of insulin-like growth factor I (IGF-I) and growth hormone (GH) on lipid metabolism have been investigated in hormonally intact rats. The subcutaneous injection of IGF-I (100 micrograms) and GH (100 micrograms) lowered total cholesterol plasma levels. The in vitro assays on isolated rat adipocytes showed a...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/0024-3205(95)02067-s
更新日期:1995-01-01 00:00:00
abstract::To determine whether rat retinal photoreceptor cells produce inhibin, a molecule closely related to activin, a multifunctional growth factor in the transforming growth factor beta superfamily (TGF beta), we have conducted immunohistochemistry using specific antibodies for inhibin which were raised against a synthetic ...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/0024-3205(95)00241-w
更新日期:1995-01-01 00:00:00
abstract::Recently, 4.4'-bis(1-p-carboxyphenyl-3-methyl-5-dydroxyl)-pyrazol (DRD156) has been developed as a new sensitive reagent that reacts specifically with singlet oxygen. The specificity of DRD156 for singlet oxygen in a biomimetic solution (micellar solution) and the effects of its coexistence with other reagent were exa...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/s0024-3205(02)02234-8
更新日期:2002-12-06 00:00:00
abstract::The endogenous cannabinoid anandamide (AEA) exerts the majority of its effects at CB1 and CB2 receptors and is degraded by fatty acid amide hydrolase (FAAH). FAAH KO mice and animals treated with FAAH inhibitors are impaired in their ability to hydrolyze AEA and other non-cannabinoid lipid signaling molecules, such as...
journal_title:Life sciences
pub_type: 杂志文章,评审
doi:10.1016/j.lfs.2012.05.015
更新日期:2013-03-19 00:00:00
abstract::The outbreak of COVID-19 in December 2019, has become an urgent and serious public health emergency. At present, there is no effective treatment or vaccine for COVID-19. Therefore, there is a crucial unmet need to develop a safe and effective treatment for COVID-19 patients. Mesenchymal stem cells (MSCs) are widely us...
journal_title:Life sciences
pub_type: 杂志文章,评审
doi:10.1016/j.lfs.2020.118493
更新日期:2020-12-01 00:00:00
abstract::In order to clarify the role played by interleukin-2 (IL-2) in the regulation of fibroblast function, we investigated the effect of rat IL-2 and human recombinant IL-2 on 3T3 fibroblast proliferation and collagen synthesis. Fibroblasts were incubated with various concentrations of IL-2 for different periods of time. I...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/0024-3205(96)00125-7
更新日期:1996-01-01 00:00:00
abstract::It has been previously shown in anesthetized pigs that intravenous infusion of 2 microg/h of 17beta-estradiol primarily dilated renal, iliac and coronary circulations, while higher doses of the hormone were required to cause vasodilation also in the mesenteric vascular bed. In the same experimental model, a tonic beta...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/s0024-3205(99)00399-9
更新日期:1999-01-01 00:00:00
abstract::The aims of the current study were: 1) to determine if the epsilonPKC pseudosubstrate peptide (epsilonphi) (NH(2)-RKRQGAVRRRVHQVNG-COOH) could be used as an epsilonPKC-selective inhibitor in neonatal cardiac myocytes (NCMs) and 2) to determine if differences in the alpha and epsilonPKC autoinhibitory pseudosubstrate m...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/j.lfs.2003.11.016
更新日期:2004-05-07 00:00:00
abstract:AIMS:Neurocardiac dysfunction is a life-threatening systemic consequence of subarachnoid hemorrhage (SAH) that contributes to triggering delayed cerebral ischemia (DCI). This study aimed to determine the impact of dobutamine cardiac support during isoflurane postconditioning on post-SAH DCI. MAIN METHODS:Male C57BL/6 ...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/j.lfs.2016.04.020
更新日期:2016-05-15 00:00:00
abstract:BACKGROUND:Polychlorinated biphenyls (PCBs) are persistent organic pollutants that bioaccumulate in adipose tissue, disturbing its metabolism and the balance of adipokines, related to obesity. The altering secretion pattern of adipokines from the adipose tissue and the increasing mechanical load in weight-bearing joint...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/j.lfs.2016.06.010
更新日期:2016-07-15 00:00:00
abstract::Meso-dimercaptosuccinic acid (DMSA) and the sodium salt of 2,3-dimercapto-1-propanesulfonic acid (DMPS) are analogous in chemical structure to dimercaprol (BAL, British Anti-Lewisite). Dimercaprol was among the first therapeutically useful metal chelating agents and was developed originally as an anti-lewisite agent. ...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/0024-3205(82)90108-4
更新日期:1982-11-08 00:00:00
abstract:AIMS:Forkhead box (FOX) proteins constitute a huge family of transcriptional regulators, which are involved in a wide range of cancers. FOXK1 is a little studied member of FOXK subfamily. This study aimed to investigate the potential prognostic value of FOXK1 in human hepatocellular carcinoma (HCC) and explore potentia...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/j.lfs.2019.04.068
更新日期:2019-07-01 00:00:00
abstract::Brain noradrenaline takes part in the regulation of several brain functions. The formation of brain noradrenaline depends on brain tyrosine (Tyr) levels, which associates with the ratio in plasma of Tyr to other large, neutral amino acids (LNAA). Tyr metabolism has been studied in users of the new generation combined ...
journal_title:Life sciences
pub_type: 临床试验,杂志文章,随机对照试验
doi:10.1016/0024-3205(94)00502-j
更新日期:1995-01-01 00:00:00
abstract::Sodium-dependent transport of D-glucose has been reported only in epithelial cells of small intestine and kidney, and well-differentiated tumors thereof. We observed a two-fold decrease (p < 0.05) in the intracellular distribution volume (Vic, defined as steady-state intracellular uptake divided by extracellular conce...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/0024-3205(96)00114-2
更新日期:1996-01-01 00:00:00
abstract::There is a growing literature indicating that opioid abuse by human addicts and opioid administration to animals have profound effects on the immune system. In the present study, implantation of morphine pellets in mice was associated with reduced phenotypic expression of the cell surface antigens specific to T-lympho...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/0024-3205(92)90607-q
更新日期:1992-01-01 00:00:00
abstract::In this study, we evaluated whether butein can inhibit the effects of tumor necrosis factor alpha (TNF-alpha), an inflammatory mediator, in intestinal epithelial HT-29 cells. Butein significantly inhibited TNF-alpha-induced interleukin 8 (IL-8) secretion and mRNA expression. Moreover, butein suppressed the expression ...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/j.lfs.2007.09.024
更新日期:2007-11-10 00:00:00
abstract:AIMS:Benidipine, a dihydropyridine Ca(2+) channel blocker, has been reported to block T-type Ca(2+) channels; however, the mechanism underlying this effect was unclear. In this study, we characterized the mechanism responsible for this blocking activity. Furthermore, the blocking activity was compared between two enant...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/j.lfs.2011.03.019
更新日期:2011-05-09 00:00:00
abstract:BACKGROUND:Cardiovascular disease (CVD) is the leading cause of mortality worldwide. Cardiac fibrosis is the scarring process occurs commonly with CVDs impairing the function and structure of heart. Herein, we investigated the role of circPAN3 in the pathogenesis of cardiac fibrosis. METHODS:A rat myocardial infarctio...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/j.lfs.2020.118015
更新日期:2020-09-15 00:00:00
abstract::The role of endothelium-derived nitric oxide (NO) to cause smooth muscle phospholamban (PLB) phosphorylation was studied in the isolated perfused rat aorta precontracted with norepinephrine using a back-phosphorylation technique. NO-induced relaxation was associated with increased PLB-phosphorylation while norepinephr...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/0024-3205(92)90357-u
更新日期:1992-01-01 00:00:00
abstract::Synthetic human pancreatic Growth Hormone-Releasing Factor (hpGRF) elevated the plasma concentration of growth hormone (GH) in young and adult domestic fowl. This in vivo effect of hpGRF appeared to be largely similar for both the 32 amino-acid (hpGRF 1-32) or 40 amino-acid (hpGRF 1-40) polypeptide, although the effec...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/0024-3205(84)90083-3
更新日期:1984-03-19 00:00:00
abstract::Dehydroepiandrosterone (DHEA), the major secretory product of the human adrenal cortex, significantly declines with advanced age. We have previously demonstrated that DHEA prevents the reduction in non-amyloidogenic APP processing, following prolonged stimulation of the muscarinic receptor, in PC12 cells that express ...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/0024-3205(96)00496-1
更新日期:1996-01-01 00:00:00
abstract::Recently, single-dose drug packaging systems, allowing the administration of multiple drugs in a single pill, have become popular for the convenience of the patient. The quality of drugs and an accurate measurement of their photostabilities within this system, however, have not been carefully addressed. Drugs that are...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/j.lfs.2005.04.033
更新日期:2005-12-12 00:00:00
abstract::PD 128907 [4a R, 10 b R-(+)-trans-3, 4, 4a, 10 b - tetrahydro - 4- n -propyl2 H,5H-[1]benzop-yrano[4,3-b]1,4-oxazin-9-ol.], a selective dopamine (DA) D3 receptor agonist ligand exhibits about a 1000-fold selectivity for human D3 receptors (Ki, 1 nM) versus human D2 receptors (Ki, 1183 nM) and a 10000-fold selectivity ...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/0024-3205(95)02102-o
更新日期:1995-01-01 00:00:00
abstract::Effects of 1-methyl-4-phenylpyridine(MPP+), a putative neurotoxic metabolite of 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine(MPTP), on the contents of dopamine were examined in the various regions of the rat brain. Under anesthesia with pentobarbital sodium and flunitrazepam, MPP+ 150 micrograms/rat was intracerebrove...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/0024-3205(89)90625-5
更新日期:1989-01-01 00:00:00
abstract::The present study is undertaken to investigate whether the phospholipase A(2) (PLA(2)) influences mRNA nucleocytoplasmic transport evaluated by nucleoside triphosphatase (NTPase) activity and mRNA export in isolated hepatic nuclear envelope. Isolated hepatic nuclei from rat liver were exposed to PLA(2) (10(-5) approxi...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/s0024-3205(03)00357-6
更新日期:2003-07-11 00:00:00
abstract::Acute myocardial infarction is associated with high rates of morbidity and mortality and can cause irreversible myocardial damage. Timely reperfusion is critical to limit infarct size and salvage the ischemic myocardium. However, reperfusion may exacerbate lethal tissue injury, a phenomenon known as myocardial ischemi...
journal_title:Life sciences
pub_type: 杂志文章,评审
doi:10.1016/j.lfs.2020.118628
更新日期:2021-01-01 00:00:00
abstract::Hypotensive responses to tryptophan and 5-hydroxytryptophan infusions were studied in normotensive male Sprague-Dawley rats. Results showed that 5-hydroxytryptophan but not tryptophan lowered pressure in a dose dependent way in direct relation to the production of brain serotonin and 5-HIAA. Intrinsic release of serot...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/0024-3205(85)90220-6
更新日期:1985-11-11 00:00:00
abstract:AIMS:The mechanisms underlying the changes in blood-brain barrier (BBB) integrity and the generation of seizures in childhood associated with preexisting brain lesions like cortical dysplasia (CD) are poorly understood. We investigated the effects of levetiracetam (LEV) on BBB integrity and the survival during hyperthe...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/j.lfs.2010.09.014
更新日期:2010-11-20 00:00:00
abstract::The melanocortins are a family of bioactive peptides derived from proopiomelanocortin. Those peptides, included among hormones and comprising ACTH, alpha-MSH, beta-MSH and gamma-MSH, are best known mainly for their physiological effects, such as the control of skin pigmentation by alpha-MSH, and ACTH effects on pigmen...
journal_title:Life sciences
pub_type: 杂志文章,评审
doi:10.1016/s0024-3205(03)00349-7
更新日期:2003-07-04 00:00:00