Abstract：
:Dehydroepiandrosterone (DHEA), the major secretory product of the human adrenal cortex, significantly declines with advanced age. We have previously demonstrated that DHEA prevents the reduction in non-amyloidogenic APP processing, following prolonged stimulation of the muscarinic receptor, in PC12 cells that express the ml acetylcholine-receptor. The present study examined whether this effect may be mediated via modulation of APP metabolism. It was found that DHEA treatment increases the content of membrane-associated APP holoprotein by 24%, and the accumulation of secreted APP in the medium by 63%. No increase in viable cell number nor in nonspecific protein production was observed in DHEA-treated cells. Thus, DHEA seems to increase specifically both APP synthesis and secretion. We propose that the age-associated decline in DHEA levels may be related to the pathological APP metabolism observed in Alzheimer's disease.
journal_name
Life Scijournal_title
Life sciencesauthors
Danenberg HD,Haring R,Fisher A,Pittel Z,Gurwitz D,Heldman Edoi
10.1016/0024-3205(96)00496-1subject
Has Abstractpub_date
1996-01-01 00:00:00pages
1651-7issue
19eissn
0024-3205issn
1879-0631pii
0024320596004961journal_volume
59pub_type
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