Abstract:
:Multidrug resistance-associated proteins (Mrps) are ATP-dependent transporters which transport a wide variety of anionic and cationic compounds. The purpose of this study was to determine the tissue distribution of Mrp5 and 6 in male and female Sprague-Dawley rats in various tissues, and to investigate whether the expression is altered by cholestasis or administration of microsomal enzyme inducers (MEIs). These MEIs activate six different transcriptionally-mediated pathways, and their effects on Mrp5 and Mrp6 expression were determined. The effects of bile-duct ligation, a cholestasis model, on Mrp5 and 6 expression in male rats were quantified. Mrp5 had marked expression in adrenal gland, and moderate expression in cerebral cortex, cerebellum, and stomach. The MEIs polychlorinated biphenyl (PCB)126, phenobarbital, and PCB99 slightly repressed Mrp5, but no single class of receptor agonists induced or repressed Mrp5. Bile-duct ligation tended to increase Mrp5 expression, but was not statistically significant at a 3 day timepoint. Mrp6 expression was highest in intestine, liver, and kidney. Mrp6 was slightly repressed by phenobarbital, dexamethasone, and isoniazid, but no one class of receptor agonists induced or repressed Mrp6, and expression was also unchanged bile-duct ligation. In conclusion, Mrp5 in rats is most highly expressed in the adrenal gland, whereas Mrp6 is mainly expressed in excretory organs (liver, intestine, and kidney), suggesting markedly different functions. Hepatic mRNA levels of Mrp5 or Mrp6 do not seem to be coordinately regulated along with Phase I enzymes via receptor-mediated pathways, and are not part of the hepatoprotective upregulation of basolateral transporters that occurs during cholestasis.
journal_name
Life Scijournal_title
Life sciencesauthors
Maher JM,Cherrington NJ,Slitt AL,Klaassen CDdoi
10.1016/j.lfs.2005.09.016subject
Has Abstractpub_date
2006-04-04 00:00:00pages
2219-25issue
19eissn
0024-3205issn
1879-0631pii
S0024-3205(05)00980-Xjournal_volume
78pub_type
杂志文章相关文献
LIFE SCIENCES文献大全abstract::Elevated levels of serum free fatty acids (FFA) may be the metabolic alteration in obesity that leads to insulin resistance (IR) and type 2 diabetes mellitus (DM). The obese Zucker rat (ZR) is a genetic model of juvenile-onset obesity and type 2 DM. Compared with its lean sibling, the obese ZR is hyperinsulinemic, hyp...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/s0024-3205(01)01345-5
更新日期:2001-10-19 00:00:00
abstract::The human placental villus tissue contains opioid receptors and peptides. The opioid peptides extracted from the villus tissue were fractionated using reverse-phase high performance liquid chromatography and a radio-receptor assay. The presence of dynorphin 1-8 was corroborated by mass spectrometric production of (M +...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/0024-3205(87)90099-3
更新日期:1987-05-25 00:00:00
abstract::miRNAs are a group of non-coding RNAs that have regulatory functions in post-transcriptional gene expression. These molecules play a fundamental role in cellular processes, for instance cell proliferation, apoptosis, migration, and invasion. Scientific investigations have previously established that miRNAs can either ...
journal_title:Life sciences
pub_type: 杂志文章,评审
doi:10.1016/j.lfs.2020.117865
更新日期:2020-09-01 00:00:00
abstract::Diabetic retinopathy, a common secondary complication of diabetes mellitus, involves extensive damage to the retinal microvasculature. Retina, being a susceptible target, is highly prone to hyperglycemia-induced molecular damages. PPAR receptor, chiefly gamma subtype, mediates numerous responses related to glucose met...
journal_title:Life sciences
pub_type: 杂志文章,评审
doi:10.1016/j.lfs.2016.03.054
更新日期:2016-05-15 00:00:00
abstract::Two alpha-adrenoceptor antagonists, phentolamine and 2-(beta-(4-hydroxyphenyl)-ethylaminomethyl)-tetralone (BE 2254) which are phenolic derivatives were radioiodinated after chloramine-T oxidation of Na125I and the labelled material isolated by chromatography. 125I-phentolamine does not bind selectively to alpha-adren...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/0024-3205(82)90623-3
更新日期:1982-03-15 00:00:00
abstract::10(-4) M ouabain increases aldosterone production at 3.6 and 5.4 mM potassium concentrations, whereas at high potassium concentration (7 mM) even lower concentration of ouabain (10(-8) M) decreases it. As ouabain has been shown to be produced by the adrenal cortex, a local paracrine regulation of aldosterone productio...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/0024-3205(93)90466-g
更新日期:1993-01-01 00:00:00
abstract::The effects of the d- and l-isomers of pentazocine were compared to that of racemic pentazocine on contractions of the mouse isolated vas deferens. L-pentazocine inhibited electrically evoked contractions of the mouse vas deferens (MVD) in a dose-dependent manner (ID50 0.37 +/- 0.04 microM). In contrast, d-pentazocine...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/0024-3205(86)90446-7
更新日期:1986-07-14 00:00:00
abstract::Nitric oxide (NO) may be an intercellular modulator within the central nervous system. L-arginine, which results in NO synthesis, increased food intake in mice while the inhibitor of NO synthesis, L-NG-nitro arginine (L-NO Arg) inhibited food intake in food deprived mice. L-arginine, but not D-arginine, partially reve...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/0024-3205(91)90102-h
更新日期:1991-01-01 00:00:00
abstract::The stability of epidermal growth factor (EGF) in the gastrointestinal humen of 3-day-old suckling and 45-day-old weaned pigs was examined by incubating iodine labeled recombinant human EGF (hEGF) in the gastrointestinal fluids at 37 degrees C and monitoring the generation of acid soluble radioactivity. Chromatographi...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/0024-3205(96)00285-8
更新日期:1996-01-01 00:00:00
abstract::Foscarnet inhibited noradrenaline and calcium-mediated contractions of the isolated perfused tail artery of the rat. When the noradrenaline contractile response was split into two components, where the first was due to the release of calcium from intracellular stores and the second to the influx of calcium from the ex...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/0024-3205(93)90541-a
更新日期:1993-01-01 00:00:00
abstract:AIMS:Carbon nanotube (CNT) membranes offer an exciting opportunity to mimic natural protein channels due to 1) a mechanism of dramatically enhanced fluid flow 2) ability to place 'gatekeeper' chemistry at the entrance to pores 3) the ability for biochemical reactions to occur on gatekeeper molecules and 4) an ability t...
journal_title:Life sciences
pub_type: 杂志文章,评审
doi:10.1016/j.lfs.2009.04.006
更新日期:2010-04-10 00:00:00
abstract::The in vitro and in vivo regulation of [3H]Ro 5-4864 binding to peripheral benzodiazepine receptors (PBR) by ion transport/exchange inhibitors was studied in the kidney. The potencies of 9-anthroic acid, furosemide, bumetanide, hydrochlorothiazide and SITS as inhibitors of [3H]Ro 5-4864 binding to renal membranes were...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/0024-3205(88)90464-x
更新日期:1988-01-01 00:00:00
abstract::It has been reported that naloxone, an opioid antagonist, has antiarrhythmic activity in vivo. In Langendorff perfused rat hearts, we found that ischemia-reperfusion-induced ventricular tachyarrhythmia reverted to normal sinus rhythm after the treatment with naloxone (3 approximately 10 microM). The method of voltage ...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/s0024-3205(98)00383-x
更新日期:1998-01-01 00:00:00
abstract:AIMS:Induction of heme oxygenase-1 (HO-1) has been widely accepted to be neuro-protective. This study aimed to examine whether hemin (a HO-1 inducer) attenuates neuronal damage in the hippocampus induced by orthotopic autologous liver transplantation (OALT) in adult rats. MAIN METHODS:Rats were randomly allocated into...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/j.lfs.2015.05.021
更新日期:2015-08-15 00:00:00
abstract::Intestinal mucosal capsaicin-sensitive afferent nerves mediate, in part, the mesenteric hyperemia after intraduodenal acidification. The hyperemia plays a role in protecting the duodenal mucosa against acid damage. We tested the hypothesis that bradykinin contributes to this protective hyperemia. A specific antagonist...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/s0024-3205(01)01455-2
更新日期:2002-01-04 00:00:00
abstract:AIMS:To investigate the effects of malignant fibrous histiocytoma amplified sequence 1 (MFHAS1) on cognitive dysfunction, the expression of tumor necrosis factor-α (TNF-α), interleukin (IL)-1β and amyloid β peptide (Aβ) in the hippocampus, as well as dendritic pathology in the hippocampal CA1 region in sepsis-associate...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/j.lfs.2019.116822
更新日期:2019-10-15 00:00:00
abstract:AIM:The aim of the present study was to assess and compare the effect of 17β-estradiol and two different selective estrogen receptor modulators (SERMs), tamoxifen and raloxifene, as well as a selective estrogen receptor alpha agonist, propyl-pyrazole-triol (PPT) and a selective estrogen receptor beta agonist, diarylpro...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/j.lfs.2011.03.010
更新日期:2011-05-09 00:00:00
abstract::In the present study, we investigated the effects of calmodulin, adenosine 5'-triphosphate (ATP) and pertussis toxin (PT) on phorbol ester (PMA) (a protein kinase C activator) induced inhibition of ANF-stimulated cyclic GMP formation in cells from the human renal cell line, SK-NEP-1. PMA inhibited ANF-stimulated guany...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/0024-3205(91)90508-9
更新日期:1991-01-01 00:00:00
abstract::Hyaluronic acid (HA), a major glycosaminoglycan component of the extracellular matrix, has regulatory influences on cells and cellular activities. To explore the effects of a high concentration (1 mg/mL) of high molecular weight HA (500-730 kD) on U937 macrophage growth dynamics, three factors that influence overall c...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/j.lfs.2004.02.038
更新日期:2004-11-12 00:00:00
abstract::Epidemiological studies have shown that cigarette smoke, an oxidant agent, is a risk factor for the development of diabetic nephropathy (DN), in which pathogenesis transforming growth factor beta(1) (TGFbeta(1)) plays a key role. In our experimental model we exposed mesangial cell cultures to cigarette smoke concentra...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/j.lfs.2003.12.026
更新日期:2004-06-18 00:00:00
abstract::Central administration of the selective mu opioid agonist, dermorphin, produces a concurrent diuretic and antinatriuretic response in conscious rats. To determine whether central mu opioids differentially affect the renal excretion of water and sodium, we examined changes in renal function produced by intracerebrovent...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/0024-3205(95)00065-e
更新日期:1995-01-01 00:00:00
abstract:AIMS:Currently, chronic gastritis is a high incidence of digestive diseases, along with loss of appetite, abdominal pain and diarrhea. Baicalin belongs to the major bioactive flavonoids compounds from Scutellariae Radix, it exhibited anti-inflammatory and anti-bacteria activities. Nonetheless, the protective effects of...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/j.lfs.2019.117064
更新日期:2019-12-15 00:00:00
abstract:AIMS:Ideally, mesenchymal stem cells (MSC) home to and/or remain at the site of damaged myocardium when administered after myocardial infarction. However, MSC may not remain in the heart, but instead relocate to other areas. We investigated quantitatively the distribution of labeled rat MSC, given by two routes after c...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/j.lfs.2008.07.020
更新日期:2008-09-26 00:00:00
abstract:AIMS:The pulmonary vasodilation induced by adrenomedullin may be beneficial in the acute pulmonary embolism (APE) setting. This study examined effects of adrenomedullin in sheep with microsphere-induced APE. MAIN METHODS:Twenty four anesthetized, mechanically ventilated sheep were randomly assigned into 3 groups (n=8 ...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/j.lfs.2015.08.013
更新日期:2015-10-15 00:00:00
abstract::The effects of C-reactive protein (CRP), the prototypical acute-phase reactant were studied on human polymorphonuclear leukocytes (PMNL) challenged with N-formyl-methionyl-leucyl-phenylalanine (fMLP) and platelet-activating factor (PAF). CRP at 8-64 micrograms/ml concentrations inhibited degranulation and superoxide p...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/0024-3205(89)90613-9
更新日期:1989-01-01 00:00:00
abstract:AIMS:Mancozeb is a dithiocarbamate fungicide known to be genotoxic and induces tumors in rodents at various sites. There is no report in the literature about its genotoxicity in humans. Here, we investigated the association between mancozeb exposure and induction of genotoxic and proapoptotic changes in cultured human ...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/j.lfs.2011.12.013
更新日期:2012-06-06 00:00:00
abstract:AIMS:Gastrointestinal cancers are a kind of deadly malignancy afflicting close to a million peoples worldwide. 5-Fluorouracil (5-Fu) is a main chemotherapeutic agent for cancer treatment. However, prolonged exposure of 5-Fu to cancer cells may cause chemoresistance and decrease the therapeutic potential of 5-Fu. MAIN ...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/j.lfs.2018.03.054
更新日期:2018-05-15 00:00:00
abstract::The effect of psychoactive agents with different clinical actions: three sedative neuroleptics (trifluoperazine, alimemazine tartrate, chlorpromazine), an anticholinergic agent (trihexyphenidyl hydrochloride), two tricyclic antidepressants (imipramine, desipramine) and lithium carbonate on the rotational correlation f...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/0024-3205(93)90144-r
更新日期:1993-01-01 00:00:00
abstract::The effect of local pH on the in vivo efflux of endogenous dopamine (DA) and 3,4-dihydroxyphenylacetic acid (DOPAC) following administration of d-amphetamine (AMPH) was examined in the striatum of the anesthetized rat using two bilaterally placed push-pull cannulae. At both pH 7.3 and 6.4, the baseline efflux values f...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/0024-3205(88)90332-3
更新日期:1988-01-01 00:00:00
abstract:AIMS:Acetylcholine is synthesized in more or less all mammalian cells. However, little is known about the subcellular location of acetylcholine synthesis. Therefore, in the present experiments the subcellular location of the synthesizing enzyme choline acetyltransferase (ChAT) was investigated by anti-ChAT immunogold e...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/j.lfs.2012.05.012
更新日期:2012-11-27 00:00:00