Role of phospholamban in NO/EDRF-induced relaxation in rat aorta.


:The role of endothelium-derived nitric oxide (NO) to cause smooth muscle phospholamban (PLB) phosphorylation was studied in the isolated perfused rat aorta precontracted with norepinephrine using a back-phosphorylation technique. NO-induced relaxation was associated with increased PLB-phosphorylation while norepinephrine as such was ineffective. Removal of endothelium significantly reduced PLB-phosphorylation in indomethacin treated vessels. Stimulation of NO-formation by ATP augmented PLB-phosphorylation in intact vessels but was ineffective in denuded aortas. The results indicate that PLB-phosphorylation of vascular smooth muscle plays an important role in mediating NO-dependent relaxation by enhancing Ca(++)-uptake into sarcoplasmic reticulum.


Life Sci


Life sciences


Karczewski P,Kelm M,Hartmann M,Schrader J




Has Abstract


1992-01-01 00:00:00














  • Effects of six diterpenes on macrophage eicosanoid biosynthesis.

    abstract::Six diterpenes (three clerodanes, two abietanes and one rosane) were tested for interactions with the cyclooxygenase and 5-lipoxygenase pathways of arachidonate metabolism and for effects of nitric oxide production. Two abietane diterpenes, aethiopinone and 11,12-dihydroxy-6-oxo-8,11,13-abietatriene and the rosane lag...

    journal_title:Life sciences

    pub_type: 杂志文章


    authors: de las Heras B,Abad MJ,Silván AM,Pascual R,Bermejo P,Rodríguez B,Villar AM

    更新日期:2001-12-07 00:00:00

  • The role of CREB and BDNF in neurobiology and treatment of Alzheimer's disease.

    abstract::Alzheimer's disease (AD) is the most common form of dementia worldwide. β-amyloid peptide (Aβ) is currently assumed to be the main cause of synaptic dysfunction and cognitive impairments in AD, but the molecular signaling pathways underlying its neurotoxic consequences have not yet been completely explored. Additional...

    journal_title:Life sciences

    pub_type: 杂志文章,评审


    authors: Amidfar M,de Oliveira J,Kucharska E,Budni J,Kim YK

    更新日期:2020-09-15 00:00:00

  • Effect of fish oil and vitamin E on ethanol-induced changes in membrane ATPases.

    abstract::We evaluated whether fish oil or vitamin E administration affected ethanol-induced changes in membrane ATPases. Male Wistar rats (225-250 g) were fed, through a gastric tube a liquid diet containing fish oil (25% of calories) and ethanol for one month. Another group of animals was given supplemental vitamin E (300 u/k...

    journal_title:Life sciences

    pub_type: 杂志文章


    authors: Nanji AA,Sadrzadeh SM

    更新日期:1994-01-01 00:00:00

  • A new aspect of the protective functions of the blood-brain barrier: activities of four drug-metabolizing enzymes in isolated rat brain microvessels.

    abstract::The multiple functions of the blood-brain barrier (BBB) consist mostly in membrane properties controlling the bidirectional exchange of molecules between the general circulation and the central nervous system. As lipophilic molecules are able to easily penetrate the membrane of brain microvessels endothelial cells, an...

    journal_title:Life sciences

    pub_type: 杂志文章


    authors: Ghersi-Egea JF,Minn A,Siest G

    更新日期:1988-01-01 00:00:00

  • Rapid release of multiple hormones from rat pituitaries perifused with recombinant interleukin-1.

    abstract::Previous studies demonstrated a direct action of interleukin-1 (IL-1) on release of hormones from rat anterior pituitary cells in monolayer culture. To rule out any possibility of a paracrine effect from the elevated hormones in the static monolayer system, and to examine further the dynamics of hormone release elicit...

    journal_title:Life sciences

    pub_type: 杂志文章


    authors: Beach JE,Smallridge RC,Kinzer CA,Bernton EW,Holaday JW,Fein HG

    更新日期:1989-01-01 00:00:00

  • Antihypertensive compounds with potent protein kinases inhibitory activity.

    abstract::A new indorocarbazole antibiotic SF2370 has been found in the culture broth of Actinomadura sp.; it has also been found to have the activities of antihypertension and diuresis. Derivatives of SF2370, NA0344, NA0345 and NA0346, when injected (0.1-1 mg/kg i.v.) significantly lowered the blood pressure of anesthetized no...

    journal_title:Life sciences

    pub_type: 杂志文章


    authors: Hachisu M,Hiranuma T,Koyama M,Sezaki M

    更新日期:1989-01-01 00:00:00

  • High glucose-induced ROS accumulation is a critical regulator of ERK1/2-Akt-tuberin-mTOR signalling in RGC-5 cells.

    abstract::Hyperglycemia and oxidative stress are the primary stressors that elicit mitochondria specific cell stress in diabetes. Here we hypothesized that elevated level of ROS in high glucose (HG) environment, trigger mitochondrial stress by damaging mitochondrial DNA (mtDNA), altering inflammatory mediators, and neurodegener...

    journal_title:Life sciences

    pub_type: 杂志文章


    authors: Pal S,Rao GN,Pal A

    更新日期:2020-09-01 00:00:00

  • Studies on the mechanism of enkephalin receptor down regulation.

    abstract::The association of [3H] [D-Ala2, D-Leu5] enkephalin ([3H]DADLE]) with mouse neuroblastoma cells (N4TG1) was investigated. Under identical conditions the time course, dose response curve and temperature dependence for ligand uptake were similar to those for ligand-induced receptor loss (down regulation). Uptake of [3H]...

    journal_title:Life sciences

    pub_type: 杂志文章


    authors: Blanchard SG,Chang KJ,Cuatrecasas P

    更新日期:1982-09-20 00:00:00

  • Existence of NO-triggered vagal afferent activation in the rat gastric mucosa.

    abstract:AIM:We previously reported the possible involvement of mucosal nitric oxide (NO)-triggered 5-HT release in luminal glutamate sensing by the gastric vagus nerve, and we proposed that the stomach, like the duodenum, could "taste" luminal nutrients. Nitric oxide synthase (NOS) is widely distributed in the gastric mucosa, ...

    journal_title:Life sciences

    pub_type: 杂志文章


    authors: Uneyama H,Niijima A,Kitamura A,Torii K

    更新日期:2009-12-16 00:00:00

  • Energy metabolism of the liver in brain dead dogs assessed by 31P-NMR spectroscopy and arterial ketone body ratio.

    abstract::The changes in hepatic energy state were assessed by 31P-nuclear magnetic resonance spectroscopy (31P-MRS) and arterial ketone body ratio (AKBR) in brain dead dogs. 31P-MRS and AKBR were measured before and at 3 hours after brain death. Wiggers' shock model was employed to compare the energy metabolism during hypotens...

    journal_title:Life sciences

    pub_type: 杂志文章


    authors: Kitai T,Tanaka A,Terasaki M,Okamoto R,Ozawa K,Morikawa S,Inubushi T

    更新日期:1991-01-01 00:00:00

  • Unique modulation of central 5-HT2 receptor binding sites and 5-HT2 receptor-mediated behavior by continuous gepirone treatment.

    abstract::The effect of continuous treatment with the selective 5-HT1A agonist gepirone upon 5-HT2-mediated behavior and cortical 5-HT2 receptor binding sites was examined in naive rats or rats receiving noradrenergic (DSP4) or serotonergic (5,7-DHT) lesions. Continuous administration of gepirone in non-lesioned rats for 3, 7, ...

    journal_title:Life sciences

    pub_type: 杂志文章


    authors: Yocca FD,Eison AS,Hyslop DK,Ryan E,Taylor DP,Gianutsos G

    更新日期:1991-01-01 00:00:00

  • Renal and systemic effects of synthetic atrial natriuretic factor.

    abstract::A synthetic peptide corresponding to a sequence of 26 amino acids contained in endogenous rat atrial natriuretic factor (ANF), was infused into one renal artery of anesthetized dogs for a comprehensive in vivo evaluation of the renal and systemic effects of pure ANF. The results proved conclusively that ANF acted dire...

    journal_title:Life sciences

    pub_type: 杂志文章


    authors: Seymour AA,Blaine EH,Mazack EK,Smith SG,Stabilito II,Haley AB,Napier MA,Whinnery MA,Nutt RF

    更新日期:1985-01-07 00:00:00

  • Knockdown of NRSF inhibits cell proliferation of ovarian cancer via activating Hippo pathway.

    abstract:AIMS:Ovarian cancer is the most leading cause of deaths among gynecologic malignancies, and Neuron-restrictive silencer factor (NRSF) can be upregulated or downregulated according to the type of tumor. However, the expression and function of NRSF in ovarian cancer is still unknown. MAIN METHODS:Expression of NRSF in n...

    journal_title:Life sciences

    pub_type: 杂志文章


    authors: Deng P,Zuo Y,Feng S,Li Z,Chen W,Li H,Wang X

    更新日期:2018-12-15 00:00:00

  • Adrenalectomy increases norepinephrine turnover in brown adipose tissue of obese (ob/ob) mice.

    abstract::The hyperphagia and rapid body weight gain normally observed in young obese (ob/ob) mice were abolished by removal of their adrenal glands, whereas food intake and weight gain of lean mice were not significantly affected by adrenalectomy. Adrenalectomy lowered body energy density (kcal/g carcass) in obese mice more th...

    journal_title:Life sciences

    pub_type: 杂志文章


    authors: Vander Tuig JG,Ohshima K,Yoshida T,Romsos DR,Bray GA

    更新日期:1984-04-09 00:00:00

  • Effect of streptozotocin-induced diabetes on somatostatin receptors in the anterior pituitary, hypothalamus and cerebral cortex of the male rat.

    abstract::In order to better understand the mechanisms underlying the reduction in GH secretion in diabetic rats, we have characterized and measured SRIH receptors in the hypothalamus and anterior pituitary gland 5 and 9 days after induction of diabetes in the rat. Experimental diabetes was induced by an intraperitoneal injecti...

    journal_title:Life sciences

    pub_type: 杂志文章


    authors: Pesce G,Guillaume V,Dutour A,Dadoun F,Joanny P,Steinberg J,Oliver C

    更新日期:1994-01-01 00:00:00

  • Characteristic of analgesia induced by noncatecholic phenylethylamines in mice.

    abstract::Using hot plate method, analgesia induced by noncatecholic phenylethylamines, phenethylamine, phenylethanolamine and amphetamine, was inhibited by naloxone, reserpine, apomorphine and p-chlorophenylalanine, while potentiated by haloperidol. These results suggest that phenylethylamines induced analgesia involves centra...

    journal_title:Life sciences

    pub_type: 杂志文章


    authors: Kubota K,Matsuoka Y,Sakuma M,Satoh S,Uruno T,Sunagane N

    更新日期:1982-09-20 00:00:00

  • Receptors for atrial natriuretic peptide (ANP) and regulation of thyroglobulin secretion by ANP in human thyroid cells.

    abstract::Specific binding sites for atrial natriuretic peptide (ANP) were identified and characterized in primary cultures of human thyroid cells. Saturation analysis using [125I] alpha rat ANP as the ligand showed a single class of high affinity binding (Kd = 0.2 nM) which was inhibited by atriopeptin I and the alpha -human f...

    journal_title:Life sciences

    pub_type: 杂志文章


    authors: Sellitti DF,Tseng YC,Wartofsky L

    更新日期:1989-01-01 00:00:00

  • Induction of oxidative stress by the metabolites accumulating in 3-methylglutaconic aciduria in cerebral cortex of young rats.

    abstract::3-methylglutaconic (MGT), 3-methylglutaric (MGA) and occasionally 3-hydroxyisovaleric (OHIVA) acids accumulate in a group of diseases known as 3-methylglutaconic aciduria (MGTA). Although the clinical presentation of MGTA is mainly characterized by neurological symptoms, the mechanisms of brain damage in this disease ...

    journal_title:Life sciences

    pub_type: 杂志文章


    authors: Leipnitz G,Seminotti B,Amaral AU,de Bortoli G,Solano A,Schuck PF,Wyse AT,Wannmacher CM,Latini A,Wajner M

    更新日期:2008-03-12 00:00:00

  • Nociceptin/orphanin FQ and related peptides reduce the increase in plasma corticosterone elicited in mice by an intracerebroventricular injection.

    abstract::Nociceptin/orphanin FQ (=N/OFQ), the endogenous ligand of ORL1 receptor (=NOP), has been reported to induce, in rodents, after intracerebroventricular (i.c.v.) administration, anti-stress and anxiolytic effects. We have observed that the handling of mice followed by an i.c.v. injection of saline, induced a marked incr...

    journal_title:Life sciences

    pub_type: 杂志文章


    authors: Le Cudennec C,Naudin B,Do Rego JC,Costentin J

    更新日期:2002-11-29 00:00:00

  • Drug targeting of leptin resistance.

    abstract::Leptin regulates glucose, lipid and energy homeostasis as well as feeding behavior, serving as a bridge between peripheral metabolically active tissues and the central nervous system (CNS). Indeed, this adipocyte-derived hormone, whose circulating levels mirror fat mass, not only exerts its anti-obesity effects mainly...

    journal_title:Life sciences

    pub_type: 杂志文章,评审


    authors: Santoro A,Mattace Raso G,Meli R

    更新日期:2015-11-01 00:00:00

  • Galactooligosaccharides protects against DSS-induced murine colitis through regulating intestinal flora and inhibiting NF-κB pathway.

    abstract:BACKGROUND/AIMS:Previous studies have demonstrated that Galactooligosaccharides (GOS), known as "bifidus factor", has anti-inflammatory effects. Colitis, a kind of colonic inflammatory damage could be induced by different chemicals. The pathogenesis and mechanism of colitis remains unclear, and may be related to intest...

    journal_title:Life sciences

    pub_type: 杂志文章


    authors: Chu H,Tao X,Sun Z,Hao W,Wei X

    更新日期:2020-02-01 00:00:00

  • Adenosine uptake sites in dog heart and brain; interaction with calcium antagonists.

    abstract::[3H] Nitrobenzylthioinosine (NBI) binding is characterized in dog heart and brain. Evidence is presented suggesting that [3H]NBI is binding to the adenosine uptake site in both tissues. Physiologic studies in open-chested dogs clearly demonstrate that NBI acts as a coronary vasodilator, consistent with an action at th...

    journal_title:Life sciences

    pub_type: 杂志文章


    authors: Marangos PJ,Finkel MS,Verma A,Maturi MF,Patel J,Patterson RE

    更新日期:1984-09-03 00:00:00

  • Molecular mechanisms of interplay between autophagy and metabolism in cancer.

    abstract::Autophagy is an essential mechanism of cellular degradation, a way to protect the cells under stress conditions, such as deprivation of nutrients, growth factors and cellular damage. However, in normal physiology autophagy plays a significant role in cancer cells. Current research is in progress to understand how auto...

    journal_title:Life sciences

    pub_type: 杂志文章,评审


    authors: Raj S,Chandel V,Kumar A,Kesari KK,Asthana S,Ruokolainen J,Kamal MA,Kumar D

    更新日期:2020-10-15 00:00:00

  • Effects of MFHAS1 on cognitive impairment and dendritic pathology in the hippocampus of septic rats.

    abstract:AIMS:To investigate the effects of malignant fibrous histiocytoma amplified sequence 1 (MFHAS1) on cognitive dysfunction, the expression of tumor necrosis factor-α (TNF-α), interleukin (IL)-1β and amyloid β peptide (Aβ) in the hippocampus, as well as dendritic pathology in the hippocampal CA1 region in sepsis-associate...

    journal_title:Life sciences

    pub_type: 杂志文章


    authors: Zhong J,Guo C,Hou W,Shen N,Miao C

    更新日期:2019-10-15 00:00:00

  • In vitro pharmacological profile of peptide III-BTD: a novel ligand for nociceptin/orphanin FQ and opioid receptors.

    abstract::Peptide III-BTD has been recently identified as a non-selective nociceptin/orphanin FQ receptor ligand by screening of a synthetic peptide combinatorial library. In the present study we evaluated the pharmacological profile of peptide III-BTD in isolated tissues (mouse and rat vas deferens, guinea pig ileum) sensitive...

    journal_title:Life sciences

    pub_type: 杂志文章


    authors: Bigoni R,Rizzi A,Rizzi D,Becker JA,Kieffer BL,Simonin F,Regoli D,Calo G

    更新日期:2000-12-01 00:00:00

  • Transport and toxic mechanism for aluminum citrate in human neuroblastoma SH-SY5Y cells.

    abstract::Aluminum (Al) is thought to be a risk factor for neurodegenerative disorders, but the molecular mechanism has been not clarified yet. In this study, we examined how a transport system handled transport of Al citrate, the major Al species in brain, and effect of Al citrate treatment on expression of the transporter and...

    journal_title:Life sciences

    pub_type: 杂志文章


    authors: Nagasawa K,Akagi J,Koma M,Kakuda T,Nagai K,Shimohama S,Fujimoto S

    更新日期:2006-05-30 00:00:00

  • Hyperbaric oxygen induces placental growth factor expression in bone marrow-derived mesenchymal stem cells.

    abstract::The bone marrow is home to mesenchymal stem cells (MSCs) that are able to differentiate into many different cell types. The effect of hyperbaric oxygen (HBO) on MSCs is poorly understood. Placental growth factor (PlGF) is an attractive therapeutic agent for stimulating revascularization of ischemic tissue. HBO has bee...

    journal_title:Life sciences

    pub_type: 杂志文章


    authors: Shyu KG,Hung HF,Wang BW,Chang H

    更新日期:2008-07-04 00:00:00

  • Benzodiazepine binding sites in rat interscapular brown adipose tissue: effect of cold environment, denervation and endocrine ablations.

    abstract::3H-Flunitrazepam (FNZP) binding was examined in a crude membrane fraction obtained from rat interscapular brown adipose tissue (IBAT). A single population of binding sites was apparent with dissociation constant (KD) = 0.47 +/- 0.04 microM and maximal number of binding sites (Bmax) = 31 +/- 5 prot-1. From the ...

    journal_title:Life sciences

    pub_type: 杂志文章


    authors: Gonzalez Solveyra C,Romeo HE,Rosenstein RE,Estevez AG,Cardinali DP

    更新日期:1988-01-01 00:00:00

  • Asymmetrical dimethylarginine induces dysfunction of insulin signal transduction via endoplasmic reticulum stress in the liver of diabetic rats.

    abstract:AIMS:Endoplasmic reticulum stress (ERS) as an emerging factor is involved in insulin resistance (IR), which is the pathological basis of diabetes mellitus. Accumulation of asymmetric dimethylarginine (ADMA), an endogenous inhibitor of nitric oxide synthase is associated with IR, but the underlying mechanisms have not b...

    journal_title:Life sciences

    pub_type: 杂志文章


    authors: Qiu N,Wei XM,Zhang ZJ,He YL,Zhou XK,Xiong Y

    更新日期:2020-11-01 00:00:00

  • Cytoprotective effects of calbindin-D(28k) against antimycin-A induced hypoxic injury in proximal tubular cells.

    abstract::Intracellular calcium plays an important role on the pathogenesis of hypoxia-induced cellular injury. Calbindin-D(28k), a cytosolic vitamin D-dependent calcium binding protein, can serve as a buffer to limit a surge in intracellular Ca2+ concentration ([Ca2+]i) induced by various stimulations. To evaluate the possible...

    journal_title:Life sciences

    pub_type: 杂志文章


    authors: Wu MJ,Lai LW,Lien YH

    更新日期:2002-06-21 00:00:00