Abstract:
:The role of endothelium-derived nitric oxide (NO) to cause smooth muscle phospholamban (PLB) phosphorylation was studied in the isolated perfused rat aorta precontracted with norepinephrine using a back-phosphorylation technique. NO-induced relaxation was associated with increased PLB-phosphorylation while norepinephrine as such was ineffective. Removal of endothelium significantly reduced PLB-phosphorylation in indomethacin treated vessels. Stimulation of NO-formation by ATP augmented PLB-phosphorylation in intact vessels but was ineffective in denuded aortas. The results indicate that PLB-phosphorylation of vascular smooth muscle plays an important role in mediating NO-dependent relaxation by enhancing Ca(++)-uptake into sarcoplasmic reticulum.
journal_name
Life Scijournal_title
Life sciencesauthors
Karczewski P,Kelm M,Hartmann M,Schrader Jdoi
10.1016/0024-3205(92)90357-usubject
Has Abstractpub_date
1992-01-01 00:00:00pages
1205-10issue
15eissn
0024-3205issn
1879-0631pii
0024-3205(92)90357-Ujournal_volume
51pub_type
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