Abstract：
:The purpose of this research is to elucidate the pharmacological mechanism mediating vesicating effects of sulfur mustard (HD) and identify an antidote to its action. HD causes blisters because epithelial cells lose their attachments. Epithelial cell adhesion is under control of the local cytotransmitter acetylcholine (ACh) working through the muscarinic and nicotinic receptor, mAChR and nAChR, classes expressed by epithelial cells. In this study, nitrogen mustard (NM)-a structural analog of HD-was used to elucidate the mechanism of vesicating effects of mustards in mucocutaneous tissues. NM caused cell detachment and cholinergic agents antagonized its effect. Radioligand binding inhibition experiments showed that NM binds to the ligand-binding site of ACh receptors (AChRs) of both classes. Ligation of AChRs on the cell membrane of keratinocytes (KC) and bronchial epithelial cells (BEC) with NM increased total esterolytic activity of serine proteinases (TEASP). Antagonists of both classes of AChRs, atropine and mecamylamine, diminished NM-induced changes, suggesting that the pathobiological effects of NM on KC and BEC result from an agonist-like degradation of ligated AChRs, predominantly of the muscarinic class. Thus, biological effects of NM on cell adhesion were antagonist-like, whereas its pharmacological effect on TEASP was agonist-like. These findings support a hypothesis that pharmacologic protection from the vesicating action of HD can be achieved by using cholinergic drugs.
journal_name
Life Scijournal_title
Life sciencesauthors
Grando SAdoi
10.1016/s0024-3205(03)00074-2subject
Has Abstractpub_date
2003-03-28 00:00:00pages
2135-44issue
18-19eissn
0024-3205issn
1879-0631pii
S0024320503000742journal_volume
72pub_type
杂志文章相关文献
LIFE SCIENCES文献大全abstract：AIMS:Clinical use of doxorubicin, an effective chemotherapeutic agent, has limited uses due to dose-dependent cardiac toxicity. It has been supposed that the production of free radicals and calcium ions overload can lead to cardiac toxicity. Magnesium is a cardioprotective drug which inhibits lipid peroxidation and red...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/j.lfs.2018.06.022
更新日期：2018-08-15 00:00:00
abstract：:Exposure of rats to an acute, uncontrollable stressor can increase brain norepinephrine (NE) turnover and decrease locomotor and exploratory behavior. We examined the ability of exogenous tyrosine, NE's amino acid precursor, to protect rats from developing these neurochemical and behavioral changes when stressed. Anim...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/0024-3205(84)90209-1
更新日期：1984-06-04 00:00:00
abstract：:Human T-cell lymphotropic virus type 1 (HTLV-1) is associated with adult T-cell leukemia/lymphoma (ATL) and HTLV-1-associated myelopathy/tropical spastic paraparesis (HAM/TSP). Only a limited percentage of infected individuals develop disease in response to the virus while the majority remain asymptomatic, and HAM/TSP...
journal_title：Life sciences
pub_type： 杂志文章,评审
doi：10.1016/j.lfs.2014.03.025
更新日期：2014-05-28 00:00:00
abstract：:To characterize the pharmacokinetics of protein-free granisetron in blood and brain we implanted microdialysis probes into the jugular vein and cerebral frontal cortex of the rat. Granisetron (3 or 6 mg/kg, i.v., n=6) was then administered, and microdialysates from blood and brain were collected from both sites and as...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/s0024-3205(99)00143-5
更新日期：1999-01-01 00:00:00
abstract：:The cardio- and neuroprotective effect of the pyridoindole stobadine (S) is conditioned mainly by its good radical scavenging properties. It has been showed by EPR experiment, that the ultimate product of the reaction of stobadine with hydroxyl radical is the nitroxyl radical. However, for the unsaturated dehydrostoba...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/s0024-3205(99)00440-3
更新日期：1999-01-01 00:00:00
abstract：UNLABELLED:The mechanisms by which exercise affects atherosclerotic plaque stability remain incompletely understood. We evaluated the effects of two training protocols on both atherosclerotic plaque structure and the signaling pathways involved in plaque rupture. METHODS:Male low-density lipoprotein (LDL) receptor kno...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/j.lfs.2016.04.007
更新日期：2016-05-15 00:00:00
abstract：:Hyperketonemia has been identified as an important factor in diabetic pregnancy affecting growth and development of the offspring. In order to assess the immediate metabolic alterations in embryos caused by excess ketone bodies, we studied rat embryonic neural tissue exposed to a high concentration of beta-hydroxybuty...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/s0024-3205(97)01110-7
更新日期：1998-01-01 00:00:00
abstract：:Previous results demonstrated that estradiol (E2) treatment of ovariectomized-adrenalectomized (OVX-ADX) rats increased glucocorticoid (GC) binding in brain regions. The experimental protocol was extended to the spinal cord, a GC target tissue in which ornithine decarboxylase (ODC) is markedly induced by GC treatment....
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/0024-3205(93)90460-k
更新日期：1993-01-01 00:00:00
abstract：:Endothelin is a vasoconstrictor peptide which has recently been isolated and sequenced from the vascular endothelial cells. It was reported to increase blood pressure in vivo and produce a prolonged contraction with a slow onset in vitro. The purpose of this study was to investigate whether endothelin can lower the po...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/0024-3205(90)90508-o
更新日期：1990-01-01 00:00:00
abstract：:We investigated whether nitric oxide (NO) exposure alters the balance between NO and endothelium-derived hyperpolarizing factor (EDHF) released from rat renal arteries. To produce states of acutely or chronically excessive NO, lipopolysaccharide (LPS) was administered intraperitoneally to rats in a single dose of 4 mg...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/j.lfs.2003.10.019
更新日期：2004-04-16 00:00:00
abstract：:We investigated serum levels of type III procollagen aminopeptide (CIII), 7S type IV collagen (CIV), and tissue inhibitor of metalloproteinase (TIMP) in 33 patients with type II diabetes mellitus (DM) without uremia (serum creatinine less than 1.5 mg/dl). The patients were divided into three groups based on measuremen...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/0024-3205(96)00099-9
更新日期：1996-01-01 00:00:00
abstract：:Adenosine is a nucleoside which has been shown to participate in the regulation of physiological activity in a variety of mammalian tissues, and has been recognized as a homeostatic neuromodulator. It exerts its actions via membrane-bound receptors which have been characterized using biochemical, electrophysiological ...
journal_title：Life sciences
pub_type： 杂志文章,评审
doi：10.1016/0024-3205(91)90043-b
更新日期：1991-01-01 00:00:00
abstract：:Traditionally, Ehrlich's tumor is used in experimental oncology to investigate the therapeutic capacity of different synthetic chemotherapeutic agents or to evaluate the antitumoral activity of different substances of natural origin. However, the understanding of immune mechanisms during Ehrlich carcinogenesis is stil...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/j.lfs.2020.118578
更新日期：2021-01-01 00:00:00
abstract：:In the early 1970's, some papers appeared reporting an immune response to opiates in animals treated with morphine and in heroin addicts, but further researches failed to confirm these results in humans. The aim of the present work is investigating with a newly developed enzyme immunoassay the existence of specific an...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/0024-3205(93)90656-n
更新日期：1993-01-01 00:00:00
abstract：:Doxorubicin (DOX) induced cardiotoxicity is a life-threatening side effect of chemotherapy and decreased cardiac function can present years after treatment. Despite the investigation of a broad range of pharmacologic interventions, to date the only drug shown to reduce DOX-related cardiotoxicity in preclinical studies...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/j.lfs.2019.117070
更新日期：2019-12-15 00:00:00
abstract：AIMS:Naturally-derived farnesol has been reported for its chemopreventive and chemotherapeutic efficacy in various cancers. However, the mechanism of action of farnesol is still to be elucidated. The present study demonstrates the chemopreventive potential of farnesol on 9,10-dimethylbenz(a)anthracene (DMBA)-initiated ...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/j.lfs.2009.05.008
更新日期：2009-07-31 00:00:00
abstract：AIMS:Prior work suggests that ischemic preconditioning increases the level of CD39 in the heart and contributes to cardiac protection. Therefore, we examined if targeted cardiac expression of CD39 protects against myocardial injury. MAIN METHODS:Mice with cardiac-specific expression of human CD39 (αMHC/hCD39-Tg) were ...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/j.lfs.2016.10.016
更新日期：2017-06-15 00:00:00
abstract：PURPOSE:HDAC4/5 and Smad7 are potential therapeutic targets for the onset and progression of B-cell acute lymphocytic leukemia (B-ALL) and indices for clinical prognosis. In contrast, HO-1 (heat shock protein 32) plays a key role in protecting tumor cells from apoptosis. METHODS:HDAC4/5, HO-1 and Smad7 expressions in ...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/j.lfs.2018.06.004
更新日期：2018-08-15 00:00:00
abstract：:Adult rat offspring earlier exposed to maternally ingested caffeine during both gestation and lactation were observed in an open field following acute administration of diazepam or cyclohexyladenosine. While both drugs reduced measures of locomotion and emotional reactivity, caffeine-exposed rats showed evidence of gr...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/s0024-3205(96)80009-9
更新日期：1996-01-01 00:00:00
abstract：:The antinociceptive effect of the benzomorphan class of opioid analgesics have been difficult to measure utilizing some of the standard animal pain models. This may be due, in part, to the sedative and/or motor effects associated with these drugs. In addition, it has been proposed that the major site of action for dru...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/0024-3205(86)90270-5
更新日期：1986-01-06 00:00:00
abstract：:The effect of psychoactive agents with different clinical actions: three sedative neuroleptics (trifluoperazine, alimemazine tartrate, chlorpromazine), an anticholinergic agent (trihexyphenidyl hydrochloride), two tricyclic antidepressants (imipramine, desipramine) and lithium carbonate on the rotational correlation f...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/0024-3205(93)90144-r
更新日期：1993-01-01 00:00:00
abstract：AIMS:The objective of this study is to investigate glucosamine (GlcN) as a transcriptional regulator of iNOS and other genes in association with the dynamic O-GlcNAcylation of RNA polymerase II (RNAPII). MAIN METHODS:The LPS- and/or GlcN-stimulated transcriptional activities of various Gal4-binding site/TATA-box-conta...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/j.lfs.2014.06.011
更新日期：2014-08-21 00:00:00
abstract：:We recently suggested that peroxisome proliferators (PP)-induced hepatocyte DNA synthesis may be mediated by a specific peroxisome proliferator activated receptor (PPAR). Heterodimers of the PPAR with the retinoid nuclear receptor, RXR, activate transcription after binding to DR1 response elements of the target genes....
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/0024-3205(96)00039-2
更新日期：1996-01-01 00:00:00
abstract：:5-hydroxytryptamine (5-HT) caused concentration-dependent relaxation of isolated rings from porcine vena cava contracted with alpha-methyl 5-HT or prostaglandin F2 alpha. Relaxation was not blocked by propranolol (1 micron), atropine (1 micron), indomethacin (3 microns), mepyramine (1 micron), cimetidine (1 micron), o...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/0024-3205(84)90240-6
更新日期：1984-07-30 00:00:00
abstract：AIMS:Oxidative stress and inflammatory response are major factors causing several tissue injuries in intestinal ischemia and reperfusion (I/R). Agmatine has been reported to attenuate I/R injury of various organs. The present study aims to analyze the possible protective effects of agmatine on intestinal I/R injury in ...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/j.lfs.2017.08.032
更新日期：2017-11-15 00:00:00
abstract：:Obesity leads to various changes in the body. Among them, the existing inflammatory process may lead to an increase in the production of reactive oxygen species (ROS) and cause oxidative stress. Oxidative stress, in turn, can trigger mitochondrial changes, which is called mitochondrial dysfunction. Moreover, excess nu...
journal_title：Life sciences
pub_type： 杂志文章,评审
doi：10.1016/j.lfs.2017.11.019
更新日期：2018-01-01 00:00:00
abstract：:To characterize the mechanism of the analgesic action of H1 antihistaminics the effects of a new, highly selective agonist, 2-(3-trifluoromethylphenyl)histamine dihydrogenmaleate (FMPH), and of the better known H1 agonist, 2-thiazolylethylamine (2-TEA), were studied on pain threshold by means of three different kinds ...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/s0024-3205(98)00295-1
更新日期：1998-01-01 00:00:00
abstract：AIMS:RNA regulatory genes were closely associated with tumorigenesis and prognosis in multiple tumors. Copy number variation (CNV) is a frequent characteristic in soft tissue sarcomas (STS). However, little is known regarding their possible roles in STS. MAIN METHODS:RNA sequence profiles and CNV data of 255 STS patie...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/j.lfs.2020.118734
更新日期：2021-01-15 00:00:00
abstract：:The effects of free fatty acids in the blood on osmotic fragility and water content of erythrocytes when combined with hyperthermia were investigated. The isotonic buffer, containing the fatty acid, was added to the erythrocyte suspension to a final concentration of 200 microM/L. The samples were kept for one hour in ...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/s0024-3205(97)01184-3
更新日期：1998-01-01 00:00:00
abstract：:Nitric oxide (NO) may play an important regulatory role in airway function. We have, thus, investigated in vitro whether epithelium derived NO may modulate cholinergic neurotransmission, via release of NO in guinea pig trachea, by using L-arginine (L-ARG), a precursor of NO synthesis, and L-N(G)-nitro-arginine-methyl-...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/0024-3205(95)00053-9
更新日期：1995-02-17 00:00:00
