Platelet-activating factor stimulates production of prostaglandin E2 in murine osteoblast-like cell line MC3T3-E1.


:We found that platelet-activating factor (PAF) stimulated the production of prostaglandin (PG) E2 in MC3T3-E1 cells in a time- and dose-dependent manner. 1.0 microM PAF gave a maximal stimulation of PGE2 production by MC3T3-E1 cells after a 4 hr PAF-treatment. Furthermore, the PAF-induced PGE2 production was abolished by the pre-treatment of the cells with a PAF receptor antagonist, 1-O-hexadecyl-2-acetyl-sn-glycero-3-phospho(N,N,N-trimethyl)hexanolamine, which occupied the same receptor site as PAF. These results suggest that PAF stimulates the PGE2 synthesis through a PAF receptor mediated pathway. Possibly PAF modulates bone metabolism by stimulating PGE2 synthesis.


Life Sci


Life sciences


Shibata Y,Ogura N,Moriya Y,Abiko Y,Izumi H,Takiguchi H




Has Abstract


1991-01-01 00:00:00














  • Production of growth related oncogene protein-alpha in human umbilical vein endothelial cells stimulated with soluble interleukin-6 receptor-alpha: role of signal transducers, janus kinase 2 and mitogen-activated kinase kinase.

    abstract::Growth-related oncogene protein-alpha (GRO-alpha) is a member of the C-X-C chemokine family with a wide variety of biological activities. We studied the production of GRO-alpha by human umbilical vein endothelial cells (HUVEC) in response to the stimulation with soluble form of interleukin-6 receptor alpha (sIL-6R). s...

    journal_title:Life sciences

    pub_type: 杂志文章


    authors: Matsumiya T,Imaizumi T,Itaya H,Shibata T,Yoshida H,Sakaki H,Kimura H,Satoh K

    更新日期:2002-05-17 00:00:00

  • Differential down-regulation of alpha-2 adrenergic receptor subtypes.

    abstract::The regulation of G protein-coupled receptor expression is important in the physiology of an organism and can occur at any of the steps between gene transcription to turnover of the receptor protein itself. Agonist stimulation causes receptor desensitization, which is characterized by a rapid reduction in response to ...

    journal_title:Life sciences

    pub_type: 杂志文章,评审


    authors: Heck DA,Bylund DB

    更新日期:1998-01-01 00:00:00

  • Involvement of asymmetric dimethylarginine (ADMA) in glomerular capillary loss and sclerosis in a rat model of chronic kidney disease (CKD).

    abstract:AIMS:Asymmetric dimethylarginine (ADMA), an endogenous nitric oxide synthase inhibitor, has been reported to be a novel marker for the progression of chronic kidney disease (CKD). We have recently found that accumulation of ADMA could trigger peritubular capillary loss, thus contributing to tubulointerstitial ischemia ...

    journal_title:Life sciences

    pub_type: 杂志文章


    authors: Ueda S,Yamagishi S,Matsumoto Y,Kaida Y,Fujimi-Hayashida A,Koike K,Tanaka H,Fukami K,Okuda S

    更新日期:2009-06-05 00:00:00

  • Estrogens influence cholecystokinin stimulated pancreatic amylase release and acinar cell membrane cholecystokinin receptors in rat.

    abstract::This study examines the influence of ovariectomy and administration of a pharmacologic dose of estradiol on amylase release from isolated-dispersed rat pancreatic acini and cholecystokinin receptors on rat acinar cell membranes. Rats were sham ovariectomized (intact) or ovariectomized (Ovx) and 21 day timed release pe...

    journal_title:Life sciences

    pub_type: 杂志文章


    authors: Blevins GT Jr,Huang HS,Tangoku A,Mckay DW,Rayford PL

    更新日期:1991-01-01 00:00:00

  • Periodic acceleration (pGz) prior to whole body ischemia reperfusion injury provides early cardioprotective preconditioning.

    abstract:AIMS:Periodic acceleration (pGz) is a method that applies repetitive sinusoidal head-to-foot motion to the horizontally positioned body. pGz adds pulses to the circulation as a function of frequency, thereby increasing shear stress to the endothelium. Pulsatile shear stress increases release of cardioprotective endothe...

    journal_title:Life sciences

    pub_type: 杂志文章


    authors: Adams JA,Wu H,Bassuk JA,Arias J,Uryash A,Jorapur V,Lamas GA,Kurlansky P

    更新日期:2010-05-08 00:00:00

  • Impairment of testicular function in electronic cigarette (e-cig, e-cigs) exposed rats under low-voltage and nicotine-free conditions.

    abstract::Despite the lack of knowledge of the effects of electronic cigarettes (e-cigarettes, e-cigs) on public health, they have been proposed as a part of smoking cessation efforts. Recently, several basic scientific studies have pointed out how e-cigs can generate carcinogens, such as e-cig liquid thermal degradation by-pro...

    journal_title:Life sciences

    pub_type: 杂志文章


    authors: Vivarelli F,Canistro D,Cirillo S,Cardenia V,Rodriguez-Estrada MT,Paolini M

    更新日期:2019-07-01 00:00:00

  • High FOXK1 expression correlates with poor outcomes in hepatocellular carcinoma and regulates stemness of hepatocellular carcinoma cells.

    abstract:AIMS:Forkhead box (FOX) proteins constitute a huge family of transcriptional regulators, which are involved in a wide range of cancers. FOXK1 is a little studied member of FOXK subfamily. This study aimed to investigate the potential prognostic value of FOXK1 in human hepatocellular carcinoma (HCC) and explore potentia...

    journal_title:Life sciences

    pub_type: 杂志文章


    authors: Cao H,Chu X,Wang Z,Guo C,Shao S,Xiao J,Zheng J,Zhang D

    更新日期:2019-07-01 00:00:00

  • Antiviral effects of sophoridine against coxsackievirus B3 and its pharmacokinetics in rats.

    abstract::Coxsackievirus B3 (CVB3) is a major pathogen for acute and chronic viral myocarditis. The aim of this study was to investigate the antiviral effects of sophoridine, an alkaloid extracted from Chinese medicinal herb, Sophora flavescens, against CVB3, and the underlying pharmacokinetics. First, we determined the antivir...

    journal_title:Life sciences

    pub_type: 杂志文章


    authors: Zhang Y,Zhu H,Ye G,Huang C,Yang Y,Chen R,Yu Y,Cui X

    更新日期:2006-03-20 00:00:00

  • Effect of stobadine on cardiac injury induced by ischemia and reperfusion.

    abstract::The aim of this work was to evaluate the effect of the antioxidant stobadine on ischemia/reperfusion-induced injury of the isolated rat heart. Experiments were performed according to Langendorff. Ischemia was induced by stop-flow lasting 30 minutes and the duration of repefusion was 30 minutes. Reperfusion of the isch...

    journal_title:Life sciences

    pub_type: 杂志文章


    authors: Knezl V,Sotníková R,Okruhlicová L,Navarová J

    更新日期:1999-01-01 00:00:00

  • Mechanisms underlying the supra-maximal thermogenesis elicited by aminophylline in rats.

    abstract::Previous studies showed that acute treatment with aminophylline (AMPY) significantly elevated maximum thermogenesis and improved cold tolerance in rats and man in severe cold. However, the exact mechanism by which AMPY enhances thermogenesis was unknown. Rats receiving enprofylline (ENPRO) (1.5 and 15 mg/kg, i.p.), a ...

    journal_title:Life sciences

    pub_type: 杂志文章


    authors: Wang LC,Jourdan ML,Lee TF

    更新日期:1989-01-01 00:00:00

  • Isolation of anti-leukemia compounds from Citrus reticulata.

    abstract::In vitro effects of medicinal plant extracts from the pericarpium of Citrus reticulata (cv Jiao Gan) (PCRJ) on the growth and differentiation of a recently characterized murine myeloid leukemic cell clone WEHI 3B (JCS) were investigated. Extracts of PCRJ not only inhibited the proliferation of JCS cells in a dose depe...

    journal_title:Life sciences

    pub_type: 杂志文章


    authors: Mak NK,Wong-Leung YL,Chan SC,Wen J,Leung KN,Fung MC

    更新日期:1996-01-01 00:00:00

  • The NADPH oxidase inhibitor DPI can abolish hypoxia-induced apoptosis of human kidney proximal tubular epithelial cells through Bcl2 up-regulation via ERK activation without ROS reduction.

    abstract:AIMS:Ischemia/reperfusion injury (IRI), resulting from hypoxic damage within a graft, is the leading cause of cell death and graft rejection. In this study, we investigated whether a HIF-1α inhibitor or various antioxidants were able to prevent ischemic injury in a cellular model in which experimental hypoxia was induc...

    journal_title:Life sciences

    pub_type: 杂志文章


    authors: Song H,Han IY,Kim Y,Kim YH,Choi IW,Seo SK,Jung SY,Park S,Kang MS

    更新日期:2015-04-01 00:00:00

  • Effects of selected herbicides on cytokine production in vitro.

    abstract::To evaluate possible deleterious effects of commonly used herbicides on leukocytes, cytokine production was selected as a sensitive indicator. After in vitro exposure of human peripheral blood mononuclear cells from normal donors, the production of all 3 cytokines tested--interferon-gamma (a type 1 cytokine), interleu...

    journal_title:Life sciences

    pub_type: 杂志文章


    authors: Hooghe RJ,Devos S,Hooghe-Peters EL

    更新日期:2000-05-19 00:00:00

  • Sorbitol accumulation in heart: implication for diabetic cardiomyopathy.

    abstract::Sorbitol levels in heart were determined in streptozotocin-induced diabetic rats. Significantly higher levels were found in hearts of diabetic rats compared to normal rats. The findings are compatible with either significantly higher de novo synthesis of sorbitol in heart than is generally believed or uptake of circul...

    journal_title:Life sciences

    pub_type: 杂志文章


    authors: Nakada T,Kwee IL

    更新日期:1989-01-01 00:00:00

  • Inhibition of the transcriptional repressor LexA: Withstanding drug resistance by inhibiting the bacterial mechanisms of adaptation to antimicrobials.

    abstract:AIMS:LexA protein is a transcriptional repressor which regulates the expression of more than 60 genes belonging to the SOS global regulatory network activated by damages to bacterial DNA. Considering its role in bacteria, LexA represents a key target to counteract bacterial resistance: the possibility to modulate SOS r...

    journal_title:Life sciences

    pub_type: 杂志文章


    authors: Bellio P,Mancini A,Di Pietro L,Cracchiolo S,Franceschini N,Reale S,de Angelis F,Perilli M,Amicosante G,Spyrakis F,Tondi D,Cendron L,Celenza G

    更新日期:2020-01-15 00:00:00

  • Protection from drug-induced hepatocellular changes by pretreatment with conjugating enzyme inhibitors in rats.

    abstract::The present paper describes the role of conjugating enzymes in the development of hepatotoxicity after administration of repeated doses of a novel monoamine oxidase type-A (MAO-A) inhibitor, (5R)-3-[2-(( 1S)-3-cyano-1-hydroxypropyl)benzothiazol-6-yl]-5-methoxymethyl-2-oxazolidinone (E2011). The effects of pretreatment...

    journal_title:Life sciences

    pub_type: 杂志文章


    authors: Sato G,Aoki T,Hosokawa S,Sagami F,Tsukidate K

    更新日期:2001-05-04 00:00:00

  • Preclinical in vivo imaging for brown adipose tissue.

    abstract::Brown adipose tissue (BAT) has multiple functions in the human body, including the production of heat and increasing energy consumption. However, BAT is also related to many kinds of diseases, such as obesity and metabolic disorders. The progression of such diseases occurs at the cellular level, and thus, imaging tech...

    journal_title:Life sciences

    pub_type: 杂志文章,评审


    authors: Gu J,Wang X,Yang H,Li H,Wang J

    更新日期:2020-05-15 00:00:00

  • Computational insights into tetracyclines as inhibitors against SARS-CoV-2 Mpro via combinatorial molecular simulation calculations.

    abstract::The COVID-19 pandemic raised by SARS-CoV-2 is a public health emergency. However, lack of antiviral drugs and vaccine against human coronaviruses demands a concerted approach to challenge the SARS-CoV-2 infection. Under limited resource and urgency, combinatorial computational approaches to identify the potential inhi...

    journal_title:Life sciences

    pub_type: 杂志文章


    authors: Bharadwaj S,Lee KE,Dwivedi VD,Kang SG

    更新日期:2020-09-15 00:00:00

  • Benzodiazepine induced chemotaxis of human monocytes: a tool for the study of benzodiazepine receptors.

    abstract::Different ligands of both the "peripheral" and the "central" benzodiazepine receptors were tested for their ability to induce human monocyte chemotaxis. Only the ligands interacting with the "peripheral receptor" (diazepam and Ro 5-4864) were active, and their action was blocked by the specific antagonist PK 11-195, b...

    journal_title:Life sciences

    pub_type: 杂志文章


    authors: Sacerdote P,Locatelli LD,Panerai AE

    更新日期:1993-01-01 00:00:00

  • Drug targeting of leptin resistance.

    abstract::Leptin regulates glucose, lipid and energy homeostasis as well as feeding behavior, serving as a bridge between peripheral metabolically active tissues and the central nervous system (CNS). Indeed, this adipocyte-derived hormone, whose circulating levels mirror fat mass, not only exerts its anti-obesity effects mainly...

    journal_title:Life sciences

    pub_type: 杂志文章,评审


    authors: Santoro A,Mattace Raso G,Meli R

    更新日期:2015-11-01 00:00:00

  • Mitochondrial targeting drug lonidamine triggered apoptosis in doxorubicin-resistant HepG2 cells.

    abstract::Mitochondria play a crucial role in the induction and execution of apoptosis. Accordingly, recent suggestions have been made to use agents that directly act on mitochondria to trigger apoptosis so that drug-sensitive and-resistant tumour cells can be eliminated. To test this hypothesis, human hepatocarcinoma HepG2 and...

    journal_title:Life sciences

    pub_type: 杂志文章


    authors: Li YC,Fung KP,Kwok TT,Lee CY,Suen YK,Kong SK

    更新日期:2002-10-25 00:00:00

  • Adrenaline causes potassium influx in skeletal muscle and potassium efflux in cardiac muscle in rats: the role of Na/K ATPase.

    abstract::Previous in vitro evidence suggests that adrenaline causes K influx in skeletal muscle by stimulating a ouabain sensitive Na/K ATPase membrane pump. However in rabbits, adrenaline induced hypokalaemia was not significantly altered by pretreatment with digoxin (50 micrograms/kg). Rats were infused with adrenaline or sa...

    journal_title:Life sciences

    pub_type: 杂志文章


    authors: Struthers AD,Davies DL,Harland D,Price JS,Brown RA,Quigley C,Brown MJ

    更新日期:1987-01-05 00:00:00

  • Glutamate metabotropic receptors modulate the expression of in vitro epileptiform activity in rat hippocampal slices.

    abstract::The effects of the mixed class I and II mGLUR agonist (+) 1S,3R-trans-amino-cyclopentane-1,3-dicarboxylic acid (ACPD) and antagonists (+) alpha-methyl-4-carboxyphenylglycine (MCPG) and L-2-amino-3-phosphonopropionic acid (L-AP3) on the basal neuronal excitability and on the expression of in vitro epileptiform activity...

    journal_title:Life sciences

    pub_type: 杂志文章


    authors: Tebano MT,Marinelli S,Sagratella S

    更新日期:1997-01-01 00:00:00

  • The effect of ontogenetic development on the anticonvulsant activity of midazolam.

    abstract::The anticonvulsant effects and duration of protective action of midazolam against Metrazol induced seizures were studied in 528 rats aged 7,12,18,25 and 90 days. The doses of 0.025, 0.05, 0.25, 0.5 and 1.0 mg/kg were administered immediately before Metrazol (100 mg/kg in all but 18-day-old animals where 90 mg/kg were ...

    journal_title:Life sciences

    pub_type: 杂志文章


    authors: Kubová H,Mares P

    更新日期:1992-01-01 00:00:00

  • Negative regulation of the protein kinase C activator-induced ICAM-1 expression in the human bronchial epithelial cell line NCI-H292 by p44/42 mitogen-activated protein kinase.

    abstract::The role of p44/42 mitogen-activated protein kinase (MAPK) in the expression of intercellular adhesion molecule-1 (ICAM-1) in NCI-H292 cells, a human bronchial epithelial cell line, was analyzed. Treatment with the protein kinase C (PKC) activator 12-O-tetradecanoylphorbol 13-acetate (TPA) (16.2 nM) or interferon-gamm...

    journal_title:Life sciences

    pub_type: 杂志文章


    authors: Shibuya Y,Hirasawa N,Sakai T,Togashi Y,Muramatsu R,Ishii K,Yamashita M,Takayanagi M,Ohuchi K

    更新日期:2004-06-11 00:00:00

  • Excitatory amino acids inhibit stimulated phosphoinositide hydrolysis in the rat prefrontal cortex.

    abstract::In rat prefrontal cortical slices, the excitatory amino acids N-methyl-D-aspartate (NMDA), ibotenate, L-aspartate, quisqualate, kainate and L-glutamate inhibit carbachol-induced phosphoinositide hydrolysis as measured by the accumulation of [3H]inositol-1-phosphate ([3H]IP1). NMDA dose-dependently inhibited the carbac...

    journal_title:Life sciences

    pub_type: 杂志文章


    authors: Noble EP,Sincini E,Bergmann D,ten Bruggencate G

    更新日期:1989-01-01 00:00:00

  • Melatonin inhibits DNA synthesis in MCF-7 human breast cancer cells in vitro.

    abstract::The aim of the present work was to study whether physiological doses of melatonin (1nM) modified DNA synthesis in MCF-7 human breast cancer cells. Exponentially growing MCF-7 cells were incubated for 24 h with thymidine (2mM) for blocking mitosis and synchronizing the cell division cycle. Synchronization was assessed ...

    journal_title:Life sciences

    pub_type: 杂志文章


    authors: Cos S,Fernández F,Sánchez-Barceló EJ

    更新日期:1996-05-24 00:00:00

  • Role of nitric oxide in lower esophageal sphincter relaxation to swallowing.

    abstract::Studies were performed in the opossum to define the role of the L-arginine-nitric oxide (NO) pathway in lower esophageal sphincter (LES) relaxation to swallowing and vagal stimulation in viv and intramural nerve stimulation in vitro. In vivo, L-NAME, a water soluble NO synthase (NOS) inhibitor, caused antagonism of LE...

    journal_title:Life sciences

    pub_type: 杂志文章


    authors: Yamato S,Saha JK,Goyal RK

    更新日期:1992-01-01 00:00:00

  • Effects of burn injury, cold stress and cutaneous wound injury on the morphology and energy metabolism of murine brown adipose tissue (BAT) in vivo.

    abstract:AIMS:Cold stress has been shown to produce dramatic increases in 2-fluoro-2-deoxy-D-Glucose ((18)FDG) accumulation by brown adipose tissue (BAT) in rodents. However, neither the effects of other types of stress on (18)FDG accumulation nor the effects of stressors on the accumulation of tracers of other aspects of energ...

    journal_title:Life sciences

    pub_type: 杂志文章


    authors: Carter EA,Bonab AA,Hamrahi V,Pitman J,Winter D,Macintosh LJ,Cyr EM,Paul K,Yerxa J,Jung W,Tompkins RG,Fischman AJ

    更新日期:2011-07-18 00:00:00

  • Mechanisms by which the serotonergic system inhibits micturition in rats.

    abstract:AIMS:Serotonergic neurons and amino acid neurons are involved in the central nervous control of lower urinary tract function. We investigated the role of the serotonergic system in the central regulation of micturition, as well as the relationship between serotonergic neurons and amino acid neurons in the lumbosacral c...

    journal_title:Life sciences

    pub_type: 杂志文章


    authors: Kadekawa K,Nishijima S,Sugaya K,Miyazato M,Saito S

    更新日期:2009-10-07 00:00:00