Abstract：
:In frog brain membranes, equilibrium saturation binding of the opiate agonist: 3H-etorphine and of the opiate antagonist: 3H-diprenorphine is biphasic. In the presence of sodium ions (120 mM), "kinetic conversions" (unchanged Bmax) are observed: high affinity binding of the 3H-agonist is decreased while high affinity binding of the antagonist is increased. Frog brain membranes were labelled either with 3H-etorphine or with 3H-diprenorphine and solubilized with digitonin. The soluble extracts were centrifuged in sucrose gradients: two distinct components were labelled which sedimented respectively faster (approximately 12 S) and slower (approximately 10 S) than did catalase, used as marker. Pre-incubation of membrane and of 3H-ligand in the presence of 120 mM NaCl resulted in 1) considerably reduced recovery of the 12 S component in labelled form and 2) substantially enhanced labelling of the 10 S component with the 3H-antagonist. Gpp(NH)p (guanyl-5'-yl imidodiphosphate) exerted similar effects as did sodium ions. Taken together, these data suggest that the two components in question represent physically distinct agonist and antagonist forms of frog brain opiate receptors.
journal_name
Life Scijournal_title
Life sciencesauthors
Puget A,Jauzac P,Meunier JCdoi
10.1016/0024-3205(83)90477-0subject
Has Abstractpub_date
1983-01-01 00:00:00pages
199-202eissn
0024-3205issn
1879-0631pii
0024-3205(83)90477-0journal_volume
33 Suppl 1pub_type
杂志文章相关文献
LIFE SCIENCES文献大全abstract：:Fibrotic lung diseases qualify among the most dreaded irreversible interstitial pulmonary complications with progressive yet largely unpredictable clinical course. Idiopathic pulmonary fibrosis (IPF) is the most challenging prototype characterized by unknown and complex molecular etiology, severe dearth of non-invasiv...
journal_title：Life sciences
pub_type： 杂志文章,评审
doi：10.1016/j.lfs.2020.118201
更新日期：2020-10-01 00:00:00
abstract：:This study examines the role of dopaminergic mechanisms in the regulation of human pancreatic polypeptide (hPP) secretion in 11 normal male volunteers. Administration of domperidone (20 mg iv), an extracerebral inhibitor of dopamine receptors, resulted in a hPP rise (p less than 0.05) within 10 min and a peak response...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/0024-3205(82)90063-7
更新日期：1982-12-27 00:00:00
abstract：:Liquid protein diet (LPD) has been shown previously to produce maternal and fetal weight loss and fetal congenital anomalies, including cataracts and craniofacial malformations. Therefore, to examine the effects of LPD in pregnancy upon the central nervous system of pups, pregnant dams were fed either a 20% casein die...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/0024-3205(83)90867-6
更新日期：1983-04-04 00:00:00
abstract：AIMS:The aim of this study was to investigate the effects of a mood stabilizer lamotrigine (LMG) on learning and memory processes using Morris water maze (MWM), modified elevated plus maze (mEPM) and passive avoidance (PA) tests. MAIN METHODS:Balb-c mice were used. LMG were injected (20; 40mg/kg, intraperitoneal, i.p....
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/j.lfs.2012.09.020
更新日期：2012-12-17 00:00:00
abstract：:To determine how protein kinase C (PKC) activity influences properties of the tetrodotoxin-resistant sodium current (TTX-R I(Na)) in neonatal rat nodose ganglion (NG) neurons, we assessed the effects of phorbol,-12-myristate,13-acetate (PMA), one of the PKC activators, and staurosporine, one of the PKC inhibitors, on ...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/j.lfs.2005.04.053
更新日期：2005-11-19 00:00:00
abstract：:Intravenous (i.v.) or intrarenal arterial (i.r.a.) injection of big endothelin-1 (big ET-1) (1.6 nmol/kg) in anesthetized rats produced a significant increase in mean arterial pressure (MAP), a decrease in renal blood flow (RBF) and an increase in renal vascular resistance (RVR). Although the pressor responses to big ...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/0024-3205(94)90067-1
更新日期：1994-01-01 00:00:00
abstract：:Monocyte chemoattractant protein-1 (MCP-1) is a 76-amino-acid chemokine thought to be the major chemotactic factor for monocytes. MCP-1 is found in macrophage-rich areas of atherosclerotic lesions. Recent report indicates that MCP-1 is induced by glucose-stimulation, raising the important link between diabetes mellitu...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/j.lfs.2007.06.019
更新日期：2007-07-26 00:00:00
abstract：:Psychostimulants including amphetamine and cocaine induce locomotion and stereotypy and suppress eating. Although the capacity of cocaine to alter locomotion is usually viewed as related to dopamine neurotransmission, recent studies suggest that norepinephrine, acting through alpha1-adrenergic receptors (alpha1-ARs) c...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/j.lfs.2007.08.004
更新日期：2007-09-08 00:00:00
abstract：:Animal studies suggest nicotine and cannabinoids may significantly enhance the therapeutic value of neuroleptics in motor disorders. This was recently demonstrated in humans by the finding that chewing nicotine gum produced striking relief from tics and other symptoms of Tourette syndrome not controlled by neuroleptic...
journal_title：Life sciences
pub_type： 杂志文章,评审
doi：10.1016/0024-3205(89)90444-x
更新日期：1989-01-01 00:00:00
abstract：:Nitric oxide (NO) may be an intercellular modulator within the central nervous system. L-arginine, which results in NO synthesis, increased food intake in mice while the inhibitor of NO synthesis, L-NG-nitro arginine (L-NO Arg) inhibited food intake in food deprived mice. L-arginine, but not D-arginine, partially reve...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/0024-3205(91)90102-h
更新日期：1991-01-01 00:00:00
abstract：:Hemi-pituitary glands of ovariectomized rats were superfused for 4 h with either LHRH or the analog buserelin (HOE 766) at several concentrations, and thereafter with medium only for another 1.5 h. In a further experiment glands were exposed for 2.5 h to LHRH or buserelin at a single concentration (5 ng/ml) and subseq...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/0024-3205(86)90524-2
更新日期：1986-08-04 00:00:00
abstract：:Oxidative stress induced cell injury is reported to contribute to the pathogenesis of cerebral ischemia. Reactive oxygen species such as hydrogen peroxide (H2O2) and superoxide radical along with nitric oxide and peroxynitrite generated during ischemia-reperfusion injury, causes the overactivation of poly (ADP-ribose)...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/j.lfs.2006.07.034
更新日期：2006-11-10 00:00:00
abstract：BACKGROUND AND OBJECTIVES:Sepsis is a life-threatening organ dysfunction syndrome arising from uncontrolled inflammatory responses. Liver injury is a crucial factor for the prognosis of sepsis. Camptothecins (CPTs) have been reported to suppress the inflammatory response induced by sepsis. G2, a CPT-bile acid conjugate...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/j.lfs.2020.118130
更新日期：2020-09-15 00:00:00
abstract：:Glial cells are currently viewed as active partners of neurons in synapse formation. The close proximity of astrocytes to the synaptic cleft implicates that they strongly influence synapse function as well as suggests that these cells might be potential targets for neuronal-released molecules. In this review, we discu...
journal_title：Life sciences
pub_type： 杂志文章,评审
doi：10.1016/j.lfs.2011.04.005
更新日期：2011-10-10 00:00:00
abstract：:CCK-octapeptide (CCK-8) (EC50 = 0.5 nM), in the presence of Li+, increased 3H-inositol phosphate (IP) accumulation in guinea pig gastric glands prelabeled with 3H-inositol. CCK-8 desulfate, human gastrin I and pentagastrin were much less potent than CCK-8. Antagonists of CCK receptors such as proglumide, dibutyryl-c-G...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/0024-3205(85)90392-3
更新日期：1985-03-11 00:00:00
abstract：:PNA+Tempol, albumin containing conjugated (polynitroxyl albumin; PNA) and free (4-hydroxyl-2,2,6,6-tetramethyl-piperidinyl-1-oxyl; Tempol) nitroxide may protect against injury caused by reactive oxygen species. Therefore, the actions of PNA+Tempol on liver injury and inflammation induced by hepatic ischemia and reperf...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/s0024-3205(00)00907-3
更新日期：2000-11-17 00:00:00
abstract：:The kinetics of lymphocyte function associated antigen 1 (LFA-1) expression on spleen cells (SPC) and liver non-parenchymal cells (NPC), and intercellular adhesion molecule 1 (ICAM-1) expression on hepatocytes (HC) was examined in acute liver injury mice induced by a DTH reaction to picryl chloride (PCl). The peak exp...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/s0024-3205(98)00062-9
更新日期：1998-01-01 00:00:00
abstract：:Comparison of neuronal and non-neuronal membrane transport of, and neuroexcitation by, the dicarboxylic amino acids bring out provocative similarities in structural selectivity, and hence in the strategies for studying them. Parallel anomalies in stereoselectivity show for both phenomena that the recognition sites are...
journal_title：Life sciences
pub_type： 杂志文章,评审
doi：10.1016/0024-3205(83)90258-8
更新日期：1983-12-05 00:00:00
abstract：AIMS:Antenatal glucocorticoid therapy has been shown to prevent acute diseases including infant respiratory distress syndrome and reduce mortality, although little is known about the effects on cardiac function-related proteins in the fetus or neonate. We investigated whether cardiac function-related proteins were alte...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/j.lfs.2009.06.017
更新日期：2009-10-21 00:00:00
abstract：AIM:Diabetic nephropathy is the leading cause of end stage renal disease in developed countries throughout the world. The imbalance between the production of reactive oxygen species (ROS) and the antioxidant defense system is the main problem that is responsible for the progression of diabetic kidney disease. In this s...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/j.lfs.2015.04.018
更新日期：2015-08-15 00:00:00
abstract：:Dopaminergic agents ameliorate experimentally induced gastroduodenal mucosal injury, but there is no information about their effect on small intestinal mucosa. We studied the effect of L-dopa and related substances on indomethacin-induced intestinal ulceration in the rat. Ulceration was produced by s.c. injection of 3...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/0024-3205(92)90517-s
更新日期：1992-01-01 00:00:00
abstract：:The temporal relationship of changes in cAMP and cGMP to oocyte maturation was examined in proestrous hamsters (day 4). The first series of experiments showed, in normal cycling hamsters, an increase in cAMP and a decrease in cGMP at 1400 h shortly after the rise in LH with oocyte maturation beginning at 1800 h. When ...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/0024-3205(83)90726-9
更新日期：1983-10-24 00:00:00
abstract：AIMS:Antitumour effects of pentapeptide (LH) derived from donkey serum albumin hydrolysates were tested against tumour cells both in vitro and in vivo. The mechanism of LH induced tumour cell apoptosis was investigated. MAIN METHODS:Human promyelocytic leukaemia cells (HL 60) were cultured to observe inhibition in vit...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/j.lfs.2013.02.013
更新日期：2013-05-20 00:00:00
abstract：:Nitric oxide (NO) is a key mediator in many physiological processes and one of the major receptors through which NO exerts its effects is soluble guanylyl cyclase. Guanylyl cyclase converts GTP to cyclic GMP as part of the cascade that results in physiological processes such as smooth muscle relaxation, neurotransmiss...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/s0024-3205(02)02361-5
更新日期：2003-01-17 00:00:00
abstract：:Noradrenaline is taken up in noradrenergic neurones via a desipramine-sensitive transport system (uptake1). There are reports which describe the activity of uptake1 being increased by extracellular ATP and ATP gamma S. This was attributed to a protein kinase-mediated mechanism. We reevaluated this phenomenon since a m...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/0024-3205(94)00665-2
更新日期：1994-01-01 00:00:00
abstract：:Proteinase inhibitors were tested for their ability to inhibit prolactin (PRL) and growth hormone (GH) release by cultured anterior pituitary cells of the rat. Inhibitors of microbial origin (chymostatin, elastatinal, leupeptin) had either no or a moderate effect on hormone release while some tripeptide aldehydes, esp...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/0024-3205(85)90636-8
更新日期：1985-02-11 00:00:00
abstract：:In 6-[18F]fluoro-L-dopa (Fdopa)/positron emission tomography (PET) studies, carbidopa pretreatment increases the Fdopa bioavailability to the brain and enhances the intensity of striatal PET images. Different PET research teams have used various carbidopa doses and routes of administration in non-human primate studies...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/0024-3205(95)00147-x
更新日期：1995-01-01 00:00:00
abstract：:We investigated the mechanism underlying the priming effect of TNF-alpha on fMLP-stimulated superoxide production in human neutrophils. TNF-alpha enhanced fMLP-stimulated superoxide production in a concentration-dependent manner. TNF-alpha also induced sphingomyelin (SM) hydrolysis and increased the formation of its m...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/s0024-3205(99)00587-1
更新日期：2000-01-01 00:00:00
abstract：AIMS:Tianeptine is an atypical antidepressant drug that has a different mechanism of action than other antidepressants. Olanzapine is an atypical antipsychotic drug used for the treatment of schizophrenia. The present study was undertaken to investigate effects of chronic administration of tianeptine or olanzapine on u...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/j.lfs.2012.09.023
更新日期：2012-12-17 00:00:00
abstract：:The ontogenetic development of [3H]-spiroperidol binding sites was measured in the optic tectum, cerebellum, forebrain base, and forebrain roof of 1-, 4-, and 16-day-old chicks. In the chick optic tectum and cerebellum both the density and the total number of [3H]-spiroperidol binding sites increased from 4- to 16-day...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/0024-3205(89)90331-7
更新日期：1989-01-01 00:00:00
