Abstract：
:We have previously shown that matrix metalloproteinases (MMPs) play a role in osteoclastic bone resorption by facilitating migration of osteoclastic cells toward bone surface through matrices. Of MMPs identified so far, MMP-9 is likely the most important proteinase for the action, since osteoclasts express this enzyme at a tremendously high level. However, no direct evidence has been provided to demonstrate its contribution to bone resorption. In this study, to address this point, we used an MMP-9 antisense phosphothiorate oligodeoxynucleotide (S-ODN), which was shown to inhibit the protein synthesis of MMP-9 efficiently. We demonstrated that the antisense S-ODN inhibited osteoclastic pit formation on matrigel-coated dentine slices in a concentration-dependent manner with a maximum reduction of total pit volume by 53% at 10 microM. These results, taken together, suggest that MMP-9 is involved in osteoclastic bone resorption process possibly by facilitating migration of osteoclasts through proteoglican-rich matrices.
journal_name
Life Scijournal_title
Life sciencesauthors
Ishibashi O,Niwa S,Kadoyama K,Inui Tdoi
10.1016/j.lfs.2006.05.024subject
Has Abstractpub_date
2006-09-20 00:00:00pages
1657-60issue
17eissn
0024-3205issn
1879-0631pii
S0024-3205(06)00422-Xjournal_volume
79pub_type
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