Abstract:
:Polyinosinic polycytidylic acid (poly I:C) is an analog of double stranded RNA, which is a common replication intermediate for many viruses. It acts through a toll-like receptor (TLR3) to induce a group of cytokines that can mediate host resistance to viruses and some cancers. The effect of ethanol (EtOH) on induction of this set of cytokines has not been determined. Mice were treated with a single dose of EtOH (by gavage) at the same time as poly I:C was administered (intraperitoneally), and cytokine mRNA expression was measured by RNAse protection assay. Concentrations of IFN-alpha, IL-10, and IL-12 in the serum were measured by ELISA. A single dose of EtOH suppressed induction of mRNA for IFN-alpha, IFN-beta, IFN-gamma, IL-6, IL-9, IL-12, and IL-15. The concentrations of IFN-alpha and IL-12 in the serum were also decreased. In contrast, IL-10 was minimally induced by poly I:C alone, but it was substantially induced by poly I:C plus EtOH. Dose response and time course studies demonstrated that significant alterations of IFN-alpha, IL-10, and IL-12 expression occurred at dosages as low as 4 g/kg (a dosage previously shown to produce blood EtOH concentrations of approximately 0.2%) and that alterations persisted at least 4-6 hr after administration of EtOH. The glucocorticoid synthesis inhibitor, aminoglutethimide, diminished corticosterone levels to normal, but did not block the effects of EtOH on cytokine expression. These results demonstrate that EtOH affects the expression of poly I:C-induced cytokines and that this action is not mediated by corticosterone. These results plus previously published findings are consistent with the idea that EtOH may be a generalized suppressor of toll-like receptor signaling.
journal_name
Life Scijournal_title
Life sciencesauthors
Pruett SB,Fan R,Zheng Qdoi
10.1016/s0024-3205(02)02507-9subject
Has Abstractpub_date
2003-03-07 00:00:00pages
1825-39issue
16eissn
0024-3205issn
1879-0631pii
S0024320502025079journal_volume
72pub_type
杂志文章相关文献
LIFE SCIENCES文献大全abstract:AIMS:Constant development of chemotherapeutic strategies has considerably improved the efficiency of tumor treatment. However, adverse effects of chemotherapeutics enforce premature treatment cessation, which leads to the tumor recurrence and accelerated death of oncologic patients. Recently, sodium ascorbate (ASC) has...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/j.lfs.2019.116657
更新日期:2019-09-01 00:00:00
abstract::Human alpha-fetoprotein (AFP) was expressed in Saccharomyces cerevisiae, with a plasmid containing the cDNA sequence for human AFP fused with the rat AFP signal peptide. The recombinant AFP was purified from the yeast lysate by DEAE-cellulose and immunoaffinity chromatography. The amino acid composition and the molecu...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/0024-3205(90)90383-3
更新日期:1990-01-01 00:00:00
abstract::The secretagogue action of lipophilic, GABA-mimetic, beta-(p-chlorophenyl)-gamma-aminobutyric acid (PCPGABA) was studied in the rat in relation to body temperature. PCPGABA (2 to 8 mg/kg s.c.) stimulated acid output in the urethane-anesthetized rat whose core temperature ranged between 28 to 30 degrees C. However, thi...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/0024-3205(90)90144-g
更新日期:1990-01-01 00:00:00
abstract::The effect of the repeated or continuous administration of an analog of GH releasing factor (GH-RF), D-Tyr-1, D-Ala-2, Nle-27, GH-RF(1-29)-NH2 (DBO-29), on the subsequent response to this peptide was investigated in pentobarbital-anesthetized male rats. A sc administration of this analog induced a greater and more pro...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/0024-3205(87)90759-4
更新日期:1987-06-22 00:00:00
abstract::The effects of leucine- and methionine-enkephalin, opiate peptides, on Ca2+ efflux from cultured bovine adrenal chromaffin cells were examined. These enkephalins stimulated the efflux of 45Ca2+ from cells in a concentration-dependent manner (10(-8) M-10(-6) M). Leucine-enkephalin did not increase the intracellular fre...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/s0024-3205(99)00493-2
更新日期:1999-01-01 00:00:00
abstract:AIMS:Endurance exercise causes fatigue due to mitochondrial dysfunction and oxidative stress. In order to find an effective strategy to prevent fatigue or enhance recovery, the effects of a combination of mitochondrial targeting nutrients on physical activity, mitochondrial function and oxidative stress in exercised ra...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/j.lfs.2009.11.003
更新日期:2010-01-02 00:00:00
abstract:BACKGROUND AND AIMS:Dental pulp stem cells (DPSC) are promising tools in regenerative medicine due to their differentiation potential and immunomodulatory properties. However, it is not clearly known whether or not DPSCs maintain their immunosuppressive effects after differentiation. In the present study, we examined t...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/j.lfs.2018.11.040
更新日期:2019-01-01 00:00:00
abstract:AIMS:C-reactive protein (CRP) is an inflammatory cytokine that has been shown to be an independent predictor of future atherothrombotic events. Hyperactivity of endothelin-1 (ET-1), a potent vasoconstrictor peptide produced by the endothelium, is linked with cardiovascular disease development and progression. ET-1 is s...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/j.lfs.2015.12.030
更新日期:2016-08-15 00:00:00
abstract::The prototypic arylpiperazines, meta-chlorophenylpiperazine (mCPP), meta-trifluoromethylphenylpiperazine (TFMPP) and quipazine are widely studied serotonergic ligands with nonselective effects at 5HT1 and 5HT2 receptor subtypes. The present study was designed to compare the affinities of these arylipiperazines at 5HT3...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/0024-3205(92)90372-v
更新日期:1992-01-01 00:00:00
abstract:AIMS:The aim of this study was to evaluate the antinociceptive (acute assays) and anti-inflammatory (chronic assays) effects of kramecyne (KACY), a peroxide isolated from Krameria cytisoides. MAIN METHODS:The antinociceptive activity of KACY was evaluated using the hot plate, acetic acid and formalin tests. The effect...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/j.lfs.2014.11.015
更新日期:2015-01-15 00:00:00
abstract::Gluten exorphin B5 (GE-B5) is a food-derived opioid peptide identified in digests of wheat gluten. We have recently shown that GE-B5 stimulates prolactin (PRL) secretion in rats; this effect is abolished by preadministration of the opioid receptor antagonist naloxone. However, since the structure of naloxone allows it...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/j.lfs.2004.09.023
更新日期:2005-02-25 00:00:00
abstract:AIMS:There have been recent reports that reconsolidation-based interventions attenuate drug reward memories in rodents. The insular cortex (IC) is an essential part of neural circuits that underlie cue-drug memory reconsolidation. GABAergic interneurons in the IC are a potent control on network excitability and play an...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/j.lfs.2020.117655
更新日期:2020-08-01 00:00:00
abstract::Neuroblastoma x glioma hybrid cells NG 108-15 were chronically treated with opioid agonists and tested for their development of "tolerance" as evaluated by the loss of inhibitory activity of opioids upon cAMP-accumulation. Desensitization was dose-dependent and non-competitive and highly selective, since the activity ...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/0024-3205(83)90512-x
更新日期:1983-01-01 00:00:00
abstract::Triiodothyronine (T3) increases phosphoglycerate mutase (PGAM) specific activity in rat skeletal and cardiac muscles. This increase is concomitant with an increase in the proportion of phosphoglycerate mutase isozymes which contain type-M subunit. Propylthiouracil (PTU), an anti-hormone, not only decreases phosphoglyc...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/0024-3205(94)90003-5
更新日期:1994-01-01 00:00:00
abstract::The cytokeratins from human bladder and esophageal epithelia were separated using chromatographic techniques. The cytokeratins were first extracted from fresh autopsy tissue using high and low salt buffers. Urea, 8.0-9.5 M, was used to solubilize the resulting cytokeratin pellet. Imidazole was found to increase the so...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/0024-3205(90)90576-d
更新日期:1990-01-01 00:00:00
abstract::The additive effects of combined valsartan and spironolactone on plasma and cardiac aldosterone escape were evaluated in spontaneously hypertensive rats (SHRs). Twenty-four SHRs were treated with valsartan (30 mg/kg body weight per day), spironolactone (20 mg/kg body weight per day) and a combination of both for 4 mon...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/j.lfs.2004.04.012
更新日期:2004-08-27 00:00:00
abstract::We have examined the role of membrane hyperpolarization in mediating vascular hyporeactivity induced by bacterial lipopolysaccharide (LPS) in endothelial-denuded strips of rat thoracic aorta ex vivo. The injection of rats with LPS caused a significant fall of blood pressure and a severe vascular hyporeactivity to nore...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/s0024-3205(00)00978-4
更新日期:2000-12-29 00:00:00
abstract::Histogranin is a naturally-occurring pentadecapeptide with a structure 80% homologous with that a fragment-(86-100) of histone H4. First isolated from bovine adrenal medulla, the peptide was also shown to be present in the pituitary, brain, adrenal glands, blood plasma, lungs and spleen. At the subcellular level, hist...
journal_title:Life sciences
pub_type: 杂志文章,评审
doi:10.1016/0024-3205(95)00068-2
更新日期:1995-03-03 00:00:00
abstract:AIM:Myokines are associated with regulation of bone and muscle mass. However, limited information is available regarding the impact of myokines on glucocorticoid (GC) mediated adverse effects on the musculoskeletal system. This study investigates the role of myokine fibroblast growth factor-2 (FGF-2) in regulating GC-i...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/j.lfs.2019.05.022
更新日期:2019-07-15 00:00:00
abstract::Adenylate cyclase activity in pancreatic acinar cell membranes was determined in rats that had undergone a treatment with pentagastrin (250 micrograms/kg, intraperitoneal three times daily) for 1 week or that had undergone small bowel resection (90%) and were sacrified at 2 weeks, 1 month and 6 months after interventi...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/0024-3205(95)02226-9
更新日期:1995-01-01 00:00:00
abstract::The NLRP3 inflammasome regulates innate immune and inflammatory responses by promoting pro-inflammatory cytokines such as IL-18 and IL-1β. NLRP3 is one of the main factors restricting the activation of the inflammasome, which is closely related to the abundance and localization of NLRP3. A substantial number of studie...
journal_title:Life sciences
pub_type: 杂志文章,评审
doi:10.1016/j.lfs.2020.118918
更新日期:2021-02-15 00:00:00
abstract:AIM:Evidence suggests that task-specific gait training improves locomotor impairments in people with incomplete spinal cord injury (SCI); however, plastic changes in brain areas remain poorly understood. The aim of this study was to examine the possible effects of a task-specific overground gait training on locomotor r...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/j.lfs.2019.116627
更新日期:2019-09-01 00:00:00
abstract::Extracts of plasma obtained from adult humans before and immediately after physical stress were subjected to high pressure liquid chromatography (HPLC); extracts or HPLC fractions, resp., were analysed for the presence of human beta-endorphin (beta H-endorphin) or fragments thereof by several radioimmunoassays using a...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/0024-3205(83)90451-4
更新日期:1983-01-01 00:00:00
abstract::Oxymorphazone (at doses of 50-200 mg/kg) was found to be a relatively weak antinociceptive drug in intact frog (Rana esculenta) when acetic acid was used as pain stimulus. Frogs remained analgesic for at least 48 hrs following oxymorphazone (200 mg/kg) administration. The ligand increased the latency of wiping reflex ...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/0024-3205(89)90302-0
更新日期:1989-01-01 00:00:00
abstract::Platelet endothelial cell adhesion molecule-1 (PECAM-1, CD31) is a cell adhesion and signaling receptor that is expressed on hematopoietic and endothelial cells. PECAM-1 is vital to the regulation of inflammatory responses, as it has been shown to serve a variety of pro-inflammatory and anti-inflammatory functions. Pr...
journal_title:Life sciences
pub_type: 杂志文章,评审
doi:10.1016/j.lfs.2010.06.001
更新日期:2010-07-17 00:00:00
abstract::The in vivo effect of trazodone on the density of [(3)H]5-HT binding sites and 5-HT(1A) receptors and adenylyl cyclase (AC) response was studied in regions of rat brain. The chronic administration of trazodone (10 mg/Kg body wt, 40 days) resulted in a significant downregulation of [(3)H]5-HT binding sites and 5-HT(1A)...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/s0024-3205(02)01926-4
更新日期:2002-08-16 00:00:00
abstract::The vascular endothelium is recognized as a barrier between blood and blood vessel wall. The abnormality of vascular endothelium is critical for atherosclerosis, hypertension and diabetes. Oxidative stress, inflammation, obesity, hyperlipidemia and insulin resistance are major contributors to endothelial dysfunction i...
journal_title:Life sciences
pub_type: 杂志文章,评审
doi:10.1016/j.lfs.2016.11.005
更新日期:2016-12-15 00:00:00
abstract::The metabolic capability of hepatocytes prepared by the perfusion method (P cells) and the tissue slice method (S cells) has been compared using standardised procedures. Yields of P cells were four times greater than for S cells. Trypan blue exclusion viability and oxygen utilization were similar although the viabilit...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/0024-3205(83)90912-8
更新日期:1983-03-28 00:00:00
abstract:AIM:This study aimed to evaluate the effects of Wortmannin, an inhibitor of phosphatidylinositol 3-kinase (PI3K)/protein kinase B (Akt), on aortic hyporeactivity to Phenylephrine (Phe) and nitric oxide bioavailability associated with pregnancy in hypertensive rats. MAIN METHODS:The intact aortic rings of pregnant and ...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/j.lfs.2014.12.014
更新日期:2015-02-01 00:00:00
abstract::Carcinoembryonic antigen-related cell adhesion molecule 1 (CEACAM1), an adhesion molecule of the immunoglobulin superfamily, has been characterized as a putative tumor suppressor because it is frequently down-regulated in aggressive types of cancer cells. Recently, however, several studies have shown that CEACAM1 acti...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/j.lfs.2007.06.002
更新日期:2007-07-04 00:00:00