当前位置： SCI文献检索 > LIFE SCIENCES期刊下所有文献 > Time course for labeling of brain membrane phosphoinositides and other phospholipids after intracerebral injection of [32P]-ATP. Evaluation by an improved HPTLC procedure.

Time course for labeling of brain membrane phosphoinositides and other phospholipids after intracerebral injection of [32P]-ATP. Evaluation by an improved HPTLC procedure.

Abstract：

:An improved two-dimensional HPTLC procedure was developed for separating phospholipids including individual phosphoinositides, phosphatidic acids and plasmalogens. This procedure was used to examine the time course for uptake of label by phospholipids in brain subcellular membranes after intracerebral injection of [gamma-32P]-ATP. There were considerable differences in the phospholipid labeling pattern among different subcellular fractions. In particular, a high proportion of labeled phosphatidylinositol 4,5-bisphosphates and phosphatidic acids was found in the myelin fraction during the initial 4 hr after injection. In other subcellular fractions, labeling of phosphoinositides was maximum at 2 hr, but with prolonged time, poly-phosphoinositides started to show a decline in radioactivity whereas labeling of other phospholipids continued to show a steady increase instead. Results indicate at least two different modes for the uptake of label by brain membrane phospholipids after intracerebral injection of [32P]-ATP.

journal_name

Life Sci

journal_title

Life sciences

authors

Sun GY,Lin TN

doi

10.1016/0024-3205(89)90475-x

subject

Has Abstract

pub_date

1989-01-01 00:00:00

pages

689-96

issue

10

eissn

0024-3205

issn

1879-0631

pii

0024-3205(89)90475-X

journal_volume

44

pub_type

杂志文章

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