Abstract：
:The effect of thapsigargin, a specific inhibitor of intracellular Ca(2+)-ATPases, on endothelium-dependent relaxation was studied in the guinea pig thoracic aorta. Thapsigargin (1 nM-1 microM) produced a concentration-dependent relaxation of aortic strips precontracted by phenylephrine (0.1 microM) in the presence and absence of extracellular Ca2+. Thapsigargin (0.1 microM-10 microM) produced the concentration-dependent contraction in aortic strips with no endothelium in the presence and absence of extracellular Ca2+. The relaxant effect of thapsigargin (1 microM) was not attained in a de-endothelialised aortic strip. NG-nitro-L-arginine (10 nM-0.1 mM), a blocker of NO synthase, produced a concentration-dependent inhibition of thapsigargin-induced relaxation. Thapsigargin failed to produce vasodilation by pretreatment of aortic strips with NG-nitro-L-arginine (10 microM). Thapsigargin (1 microM) increased tissue levels of guanosine 3', 5' cyclic-monophosphate in aortic strips preincubated with phenylephrine (0.1 microM). The results suggest that the intracellular Ca2+ mobilization in endothelial cells by thapsigargin is of significance in the endothelium-dependent relaxation of guinea pig thoracic aorta.
journal_name
Life Scijournal_title
Life sciencesauthors
Matsuyama S,Shuntoh H,Katayama S,Tanaka Cdoi
10.1016/0024-3205(93)90244-wsubject
Has Abstractpub_date
1993-01-01 00:00:00pages
681-8issue
9eissn
0024-3205issn
1879-0631pii
0024-3205(93)90244-Wjournal_volume
53pub_type
杂志文章相关文献
LIFE SCIENCES文献大全abstract：:Sexual interactions have multiple effects on oxytocin systems in the brain. In the present study we observed that allowing estrogen-progesterone treated ovariectomized rats to be mounted ten times by a male significantly decreased the density (beta max) of thymic oxytocin receptors. Animals were ovariectomized and aft...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/0024-3205(93)90467-h
更新日期：1993-01-01 00:00:00
abstract：:Plasma opioid levels were determined in 9 obese non-diabetic subjects, their 8 age matched controls, and in 29 diabetic patients; 10 maintained on diet alone, 6 on an oral hypoglycemic agent (chlorpropamide) and 13 treated with insulin. Five age matched controls for the diabetic groups were also studied for comparison...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/0024-3205(84)90364-3
更新日期：1984-05-21 00:00:00
abstract：:Sphinganine (SP) pre-treatment potentiated the retinoic acid (RA)-induced (4-96h exposures) differentiation and increase of alkaline phosphatase (ALP) activity. A higher percentage of SP pre-treated cells in RA exposures resembled mature myelocytes or granulocytes; greater increase in ALP activity was observed. In cel...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/0024-3205(93)90688-y
更新日期：1993-01-01 00:00:00
abstract：:Recent studies suggest that the time of day at which food is consumed dramatically influences clinically-relevant cardiometabolic parameters (e.g., adiposity, insulin sensitivity, and cardiac function). Meal feeding benefits may be the result of daily periods of feeding and/or fasting, highlighting the need for improv...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/j.lfs.2018.01.031
更新日期：2018-03-15 00:00:00
abstract：:SDZ PGU 693 acts as a hypoglycemic agent by stimulating glucose utilisation in insulin-sensitive peripheral tissues, such as skeletal muscle and fat. In a 28 day toxicity study the compound was found to induce hepatocellular hypertrophy in Wistar rats treated with 300 mg/kg/day. To gain insights into the pathomechanis...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/s0024-3205(98)00509-8
更新日期：1998-01-01 00:00:00
abstract：:In this study, we have identified the E-NTPDase family members and ecto-5'-nucleotidase/CD73 in rat heart left ventricle. Moreover, we characterize the biochemical properties and enzyme activities from synaptosomes of the nerve terminal endings of heart left ventricle. We observe divalent cation-dependent enzymes that...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/j.lfs.2007.12.003
更新日期：2008-02-27 00:00:00
abstract：AIMS:It has been shown that exposure to extremely-low frequency (˂300Hz) oscillating electromagnetic field (EMF) can affect gene expression. The effects of different exposure patterns of 50-Hz EMF and co-treatment of EMF plus cisplatin (CDDP) on mRNA levels of seven genes involved in DNA repair pathways (GADD45A, XRCC1...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/j.lfs.2016.10.006
更新日期：2016-12-01 00:00:00
abstract：:Anandamide (arachidonylethanolamide), isolated from porcine brain, has been shown to bind to the cannabinoid receptor and also to produce cannabimimetic activity in pharmacological assays. This study examined structure-activity relationships in alkylated anandamide analogs. The analogs were evaluated for their ability...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/0024-3205(95)00187-b
更新日期：1995-01-01 00:00:00
abstract：AIMS:Despite the advanced cancer treatments, there is increased resistance to chemotherapy and subsequent mortality. In lack of reliable data in monolayer cultures and animal models, researchers are shifting to 3D cancer spheroids, which represents the in vivo robust tumour morphology. Calcium is essential in cell sign...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/j.lfs.2020.118291
更新日期：2020-11-01 00:00:00
abstract：:Silica (SiO2) nanoparticles (NPs) were synthesized by laser ablation method and were characterized by TEM and DLS techniques. Afterwards, their inhibition activity against carbonic anhydrase (CA) isoforms (CA I and CA II) was explored by experimental and theoretical analysis. Also, the protective effect of SiO2 NPs ag...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/j.lfs.2021.119032
更新日期：2021-01-12 00:00:00
abstract：:Normal mammary gland growth and reproduction were examined in C3H/HeMei mice given caffeine as drinking water (500 mg/l tap water) after weaning on day 20 of age. Caffeine ingestion had little effects on any of the day of vaginal opening, body growth, pattern of estrous cycles, mammary gland growth and reproduction ex...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/0024-3205(86)90653-3
更新日期：1986-07-28 00:00:00
abstract：:Stonustoxin (SNTX) is a 148 kDa, dimeric, hypotensive and lethal protein factor isolated from the venom of the stonefish Synanceja horrida. SNTX (10-320 ng/ml) progressively causes relaxation of endothelium-intact, phenylephrine (PE)-precontracted rat thoracic aortic rings. The SNTX-induced vasorelaxation was inhibite...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/j.lfs.2007.01.058
更新日期：2007-04-10 00:00:00
abstract：AIMS:The present study aimed to verify changes in cerebellar and plasmatic expression of miRNAs after the chronic consumption of ethanol and caffeine in the UChB rat, an experimental model for alcoholism. MATERIAL AND METHODS:Male rats at 5 months of age, were divided into the following groups (n = 10/group): 1. Ethan...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/j.lfs.2019.05.016
更新日期：2019-07-15 00:00:00
abstract：:Platelet functions are becoming the useful tool for delineating the etiology of pregnancy induced hypertension. Electronmicroscopic studies, efflux and content of 5-HT in platelets and platelet aggregation responses towards various aggregating agents have been measured in 25 normotensive pregnant subjects and 31 PIH s...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/0024-3205(94)00735-7
更新日期：1994-01-01 00:00:00
abstract：AIM:To compare embryonic developmental competence and clinical outcomes of oocytes matured in vivo (IVF oocytes) and those matured in vitro (IVM oocytes) from the same IVM/IVF cycles, and to analyze the clinical efficiency of a melatonin-supplemented in vitro maturation system combined with a modified IVM/IVF protocol....
journal_title：Life sciences
pub_type： 杂志文章,随机对照试验
doi：10.1016/j.lfs.2020.118706
更新日期：2021-01-01 00:00:00
abstract：AIM:Ethanol is one of the most widely used recreational drugs in the world. At high concentrations, it can induce carotid artery vasoconstriction. Hyperthermia potentiates its effects resulting in carotid artery vasoconstriction at any concentration. The aim of this study is to investigate the interaction between ethan...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/j.lfs.2017.08.037
更新日期：2017-11-01 00:00:00
abstract：:In this study, we investigated the effects of zinc L-carnosine, an anti-ulcer drug, on acetic acid-induced colonic mucosal injury and the correlation of these effects with expression of 72-kDa heat shock proteins (HSP72) and nuclear factor kappa B (NF-kappaB) activation in rat colonic mucosa in vivo. After intrarectal...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/j.lfs.2006.07.032
更新日期：2006-11-10 00:00:00
abstract：:Anandamide (arachidonylethanolamide; AEA) is an endogenous ligand for the cannabinoid receptor and its pharmacological effects are under intensive study. However, AEA has a low aqueous solubility and stability which may restrict its use and may eventually endanger the reliability of the obtained results. In the presen...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/0024-3205(96)00024-0
更新日期：1996-01-01 00:00:00
abstract：:23-Hydroxybetulinic acid, a derivative of betulinic acid, was investigated for its apoptotic effect and the associated telomerase activity in human leukemia HL-60 cells. Apoptosis and bcl-2 were determined by flow cytometry analysis. A PCR-based telomeric repeat amplification protocol assay was used to detect telomera...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/s0024-3205(02)02176-8
更新日期：2002-11-22 00:00:00
abstract：AIM:Preparation of pegylated gold nanorods (PEG-AuNRs) that are capable of converting near infrared (NIR) light into heat. Evaluation of cancer therapeutic efficacy and long-term toxicity of the proposed photothermal therapy in comparison with other conventional modalities. MATERIALS AND METHODS:Prepared PEG-AuNRs wer...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/j.lfs.2020.118108
更新日期：2020-09-15 00:00:00
abstract：AIMS:This study aims to explore the effect and underlying mechanism of zoledronic acid (ZA) on the incidence of thyroid cancer (TC) tumorigenesis. MATERIALS AND METHODS:Human mononuclear cells THP-1 were differentiated into M2-like tumor associated macrophages (TAMs) by incubation with PMA followed by additional incub...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/j.lfs.2020.117925
更新日期：2020-09-01 00:00:00
abstract：:The aim of this study is to investigate the effect of three cucurbitacins (Cuc) E, D and I on the bilirubin-albumin binding, both in human serum albumin (HSA) and in plasma. Bilirubin-HSA solution and plasma free of cucurbitacins were prepared as well as others containing serial concentrations of cucurbitacins. The co...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/j.lfs.2006.10.005
更新日期：2007-01-16 00:00:00
abstract：:The effects of chronic treatment with antidepressants, verapamil, or lithium on serotonin (5-HT)-induced Ca2+ increase were investigated in single C6BU-1 glioma cells with digital imaging microscopy. Clomipramine and citalopram, at a concentration of 100 nM, decreased the peak values of 5-HT-induced [Ca2+]i changes. V...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/s0024-3205(97)00010-6
更新日期：1997-01-01 00:00:00
abstract：AIMS:Tumor necrosis factor-related apoptosis-inducing ligand (TRAIL) has been considered to be one of the most promising candidates in research on treatments for cancer, including renal cell carcinoma (RCC). However, many cells are resistant to TRAIL-induced apoptosis which limits the potential application of TRAIL in ...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/j.lfs.2014.02.002
更新日期：2014-04-01 00:00:00
abstract：:Leu-enkephalin (100 micrograms/kg, i.p.) administered to mice 5 min before training in a one way active avoidance task significantly reduced the number of avoidances observed in the peptide treated animals. This impairing action of Leu-enkephalin was partially attenuated by methylnaloxonium (naloxonium), a quarternary...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/0024-3205(85)90101-8
更新日期：1985-12-23 00:00:00
abstract：:Several neuronal cell lines were screened for endogenous expression of neuronal nitric oxide synthase (nNOS) protein using Western blot analysis. Detectable levels of the nNOS protein were evident in the SK-N-SH, SH-SY5Y, and N1E-115 neuroblastoma cell lines, as well as the NG108-15 neuroblastoma x glioma hybrid. Only...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/s0024-3205(02)01732-0
更新日期：2002-07-05 00:00:00
abstract：:We evaluated the effects of FR901533, endothelin converting enzyme inhibitor, on development of right ventricular overload and medial thickening of pulmonary arteries in rats with monocrotaline-induced pulmonary hypertension. Pulmonary hypertension was induced by a single injection of monocrotaline (80 mg/kg). Twenty-...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/s0024-3205(98)00347-6
更新日期：1998-01-01 00:00:00
abstract：AIMS:The purposes of this study were to determine whether Cervi Pantotrichum Cornu (CPC) has osteogenic activities in human osteoblastic MG-63 cells and to investigate the underlying molecular mechanism. MAIN METHODS:The effects of CPC on alkaline phosphatase activity, collagen synthesis, and calcium deposits were mea...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/j.lfs.2013.11.002
更新日期：2014-01-14 00:00:00
abstract：:Three forms of alkaline phosphatase have been isolated from different sections of the small intestine: F3 180 kDa from the duodenum; F2 150 kDa principally jejunal; F1 120 kDa the only ileal form. Their catalytic properties have been compared as well as the electrophoretic properties the dimer and monomer of their pho...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/0024-3205(88)90355-4
更新日期：1988-01-01 00:00:00
abstract：:The effect of fluoride ion on the binding of the specific muscarinic agonist ligand [3H]cis methyldioxolane ([3H]CD) to the mouse cardiac muscarinic receptor was investigated. Utilizing equilibrium ligand binding experiments, sodium fluoride (10mM) was shown to decrease [3H]CD binding, measured at a concentration of 2...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/0024-3205(82)90602-6
更新日期：1982-03-08 00:00:00
