Abstract：
:The interactions of aminoglycoside, 3',4'-dideoxykanamycin B(DKB) with ATP and its related compounds were investigated. ATP, ADP, cyclic AMP and FAD bound to the DKB-conjugated Sepharose 4B column. The binding of DKB to ATP was also confirmed by equilibrium gel filtration. In the acidic pH region, the fluorescence of nucleotides was quenched by DKB. The Stern-Volmer plots showed that the molar ratios of the complexes were 1:1. The apparent stability constant was dependent on the number of the phosphate groups of nucleotides and was in the order of ATP greater than ADP greater than AMP.
journal_name
Life Scijournal_title
Life sciencesauthors
Inaba A,Aramaki Y,Takahashi M,Tsuchiya Sdoi
10.1016/0024-3205(86)90332-2subject
Has Abstractpub_date
1986-10-13 00:00:00pages
1345-51issue
15eissn
0024-3205issn
1879-0631pii
0024-3205(86)90332-2journal_volume
39pub_type
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