Abstract：
:The metabolic capability of hepatocytes prepared by the perfusion method (P cells) and the tissue slice method (S cells) has been compared using standardised procedures. Yields of P cells were four times greater than for S cells. Trypan blue exclusion viability and oxygen utilization were similar although the viability of S cells deteriorated faster with time. P cells had a lower endogenous rate of glycogenolysis and showed better glucagon stimulation than S cells. Similarly, P cells performed gluconeogenesis at a higher rate. However, there was no significant differences in the metabolism of the xenobiotic ethoxycoumarin. It is concluded that while S cells are probably satisfactory for studies of drug metabolism their use for work involving surface receptor binding and energy demanding processes should be questioned.
journal_name
Life Scijournal_title
Life sciencesauthors
Sturdee AP,Beirne JM,Sörman AE,Orton TC,Crisp DMdoi
10.1016/0024-3205(83)90912-8subject
Has Abstractpub_date
1983-03-28 00:00:00pages
1463-9issue
13eissn
0024-3205issn
1879-0631pii
0024-3205(83)90912-8journal_volume
32pub_type
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