Abstract：
BACKGROUND:Gastric cancer is a severe disease with a high occurrence rate worldwide. And lncRNAs are demonstrated to be responsible for cancer growth and metastasis. So, it is of great importance to explore the lncRNAs involved mechanism of gastric cancer occurrence and development deeply. METHODS:Transfection was conducted to build over-expression and down-expression models. Moreover, RT-qPCR and western blot were used to detect the transcriptional and translational levels. The biological functions such as proliferation, migration and invasion of AGS cells were evaluated by MTT analysis, colony formation assay, scarification detection and transwell assay, respectively. The potential binding of miR-135b and its downstream and upstream molecules was validated by dual luciferase reporter gene assay or RIP. Also, the in-vivo mice model was further used to demonstrate the role of lncRNA PCAT18 in gastric cancer. RESULTS:PCAT18 down-expression promoted proliferation, migration and invasion of gastric cancer cells. Furtherly, over-expression of miR-135b also promoted these biological characteristics of AGS cells. Importantly, we found that PCAT18 could bind miR-135b which also was bound with CLDN11. We found that miR-135b is negatively correlated with CLDN11; PCAT18 and CLDN11 are positively correlated. Moreover, miR-135b mimics could down-regulate protein level of CLDN11, whereas CLDN11 could reverse this effect. In in-vivo experiment, PCAT18 over-expression restrained tumor growth and metastasis. CONCLUSIONS:Over-expressed lncRNA PCAT18 inhibits proliferation, migration and invasion of gastric cancer cells through regulation of miR-135b/CLDN11.
journal_name
Life Scijournal_title
Life sciencesauthors
Zhang XZ,Mao HL,Zhang SJ,Sun L,Zhang WJ,Chen QZ,Wang L,Liu HCdoi
10.1016/j.lfs.2020.117478subject
Has Abstractpub_date
2020-05-15 00:00:00pages
117478eissn
0024-3205issn
1879-0631pii
S0024-3205(20)30226-5journal_volume
249pub_type
杂志文章相关文献
LIFE SCIENCES文献大全abstract：AIMS:Sarcopenia is characterized by muscle mass and strength loss and reduced physical activity. Branched-chain amino acids (BCAAs) were recently described as an activator of protein synthesis via mammalian target of rapamycin (mTOR) signaling for muscle atrophy. In cardiovascular diseases, excessive activation of the ...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/j.lfs.2020.117593
更新日期：2020-06-01 00:00:00
abstract：:SDZ PGU 693 acts as a hypoglycemic agent by stimulating glucose utilisation in insulin-sensitive peripheral tissues, such as skeletal muscle and fat. In a 28 day toxicity study the compound was found to induce hepatocellular hypertrophy in Wistar rats treated with 300 mg/kg/day. To gain insights into the pathomechanis...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/s0024-3205(98)00509-8
更新日期：1998-01-01 00:00:00
abstract：:The effects of tetrandrine (TET) on the contractile responses of rat aortic rings and perfused rat mesenteric arteries to phenylephrine (PE) were investigated. TET inhibited the maximal contraction to PE in a concentration-dependent manner. TET significantly inhibited the transient contraction in Ca(2+)-free medium pr...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/s0024-3205(96)00552-8
更新日期：1996-01-01 00:00:00
abstract：AIMS:This study aims to develop and evaluate oleuropein loaded surface functionalized folate-targeted - PEG liposomes for the effective management of prostate cancer in an animal model. MATERIALS AND METHODS:Film hydration-cum-extrusion technique was used to produce liposomes. Particle size, entrapment efficiency, dru...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/j.lfs.2019.01.053
更新日期：2019-03-01 00:00:00
abstract：:This study examines the effects of prenatal cocaine administration on the development of vascular sympathetic innervation and contractile responsiveness. Rabbits received cocaine (4 mg/kg, iv, bid) or saline during gestational days 8 to 29. Aortas were obtained on postnatal days 10, 20, 30 and 50. Vascular smooth musc...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/0024-3205(94)00765-9
更新日期：1994-01-01 00:00:00
abstract：:Based on the finding of decreased mitochondrial complex I activity in the substantia nigra of patients with Parkinson's disease, we propose that the consequent reduction of ATP synthesis and increased generation of reactive oxygen species may be a possible cause of nigrostriatal cell death. Since sulfhydryl groups are...
journal_title：Life sciences
pub_type： 杂志文章,评审
doi：10.1016/s0024-3205(98)00472-x
更新日期：1999-01-01 00:00:00
abstract：:3-methylglutaconic (MGT), 3-methylglutaric (MGA) and occasionally 3-hydroxyisovaleric (OHIVA) acids accumulate in a group of diseases known as 3-methylglutaconic aciduria (MGTA). Although the clinical presentation of MGTA is mainly characterized by neurological symptoms, the mechanisms of brain damage in this disease ...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/j.lfs.2007.12.024
更新日期：2008-03-12 00:00:00
abstract：:Brief electrical pulses applied to the rat tail elicit a complex vocal response which includes audible (peeps, chatters) and ultrasonic (USV) components. Aspirin and amitriptyline had no effect on the vocal responses. Morphine showed a dose-dependent and naloxone reversible antinociceptive effect on the 1st and 2nd au...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/s0024-3205(98)00450-0
更新日期：1998-01-01 00:00:00
abstract：:Anorexia, nausea/emesis and peripheral sensorial neuropathy are frequent adverse effects associated with chemotherapy. Cannabinoids have been proposed to alleviate these effects, but their preventive properties in long-term experimental models have not been tested. This study was conducted to determine whether or not ...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/j.lfs.2007.06.012
更新日期：2007-07-19 00:00:00
abstract：:The role of serotonin (5-HT) in the regulation of steroidogenesis in the ovary via 5-HT2 receptor was studied in normal cycling rats and their preovulatory follicles. Oral administration of ketanserin (KET), a selective 5-HT2 antagonist, at 10 mg/kg BW at 1100h on the day of proestrus resulted in a marked decrease in ...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/0024-3205(93)90713-d
更新日期：1993-01-01 00:00:00
abstract：:The loss of gamma-aminobutyric acid (GABA)-ergic medium spiny neurons (MSNs) in the striatum is the hallmark of Huntington disease (HD), an incurable neurodegenerative disorder characterized by progressive motor, psychiatric, and cognitive symptoms. Transplantation of MSNs or their precursors represents a promising tr...
journal_title：Life sciences
pub_type： 杂志文章,评审
doi：10.1016/j.lfs.2018.07.030
更新日期：2018-09-15 00:00:00
abstract：:The effects of pyrroloquinoline quinone (PQQ) and PQQ-oxazole (PQQ-glycine adduct) on DNA synthesis were examined using cultured human fibroblasts. Confluent fibroblasts were cultured in serum-free Dulbecco's modified Eagle's media, and various concentrations of PQQ and PQQ-oxazole were added to the media. After incub...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/0024-3205(93)90631-c
更新日期：1993-01-01 00:00:00
abstract：:Amino acid and reduced glutathione (GSH) levels in substantia nigra (SN) as well as striatal monoamine levels were measured in acetyl-L-carnitine (ALCar) treated and control Swiss-Webster mice. ALCar, L carnitine, or saline were administered i.p. to mice for 5 days and mice were decapitated 24 hours following the last...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/0024-3205(88)90319-0
更新日期：1988-01-01 00:00:00
abstract：:The discovery of CB1 and CB2 receptors and of endogenous agonists for these receptors has sparked renewed interest in the therapeutic potential of cannabinoids. This has led to a need for strategies that will provide a better separation of wanted from unwanted effects, particularly for CB1 receptor agonists. Possible ...
journal_title：Life sciences
pub_type： 杂志文章,评审
doi：10.1016/s0024-3205(99)00282-9
更新日期：1999-01-01 00:00:00
abstract：:The effects of aging on contractile response to angiotensin II and tachyphylaxis to it were investigated using aortic strips from rats aged 1.5, 4 and 22 months. Whether the endothelium was present or not, the contractile response to angiotensin II was greater and tachyphylaxis to it was less in 1.5-month-old rats tha...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/0024-3205(90)90549-7
更新日期：1990-01-01 00:00:00
abstract：:Amyloid beta protein (Abeta) alters signal transduction systems, including increases in the cytosolic free calcium ([Ca2+]i) response which have pathophysiological significance in Alzheimer's disease (AD). The purposes of this study were to elucidate the mechanism involved in Abeta's effect on the Ca2+ signal and to e...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/s0024-3205(00)00470-7
更新日期：2000-01-01 00:00:00
abstract：:Early induction of VEGF was studied in liver, kidney and lung of spontaneously diabetic rats. Western blot analysis, northern hybridization were applied to show the expression of VEGF in different organs. Radiolabelled hypoxia responsive element (HRE) and cAMP responsive element (CRE) oligonucleotides were assayed by ...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/s0024-3205(01)01327-3
更新日期：2001-10-12 00:00:00
abstract：:Fluorescence spectroscopy was used to examine the biophysical characteristics of human platelet membranes as a function of subject age. The structural order of membrane lipid domains was determined with the use of 1,6-diphenyl-1,3,5-hexatriene (DPH), a fluorescent probe that preferentially localizes in the hydrocarbon...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/0024-3205(85)90079-7
更新日期：1985-10-14 00:00:00
abstract：:We investigated the mechanism by which glucose refeeding can reverse the enhancement of glycolysis caused by glucose starvation. Human fibroblasts were deprived of glucose for 18 hr and then refed for 1 hr with either (a) medium from sister glucose-starved cultures (controls), (b) fresh, glucose-containing medium (fre...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/0024-3205(89)90057-x
更新日期：1989-01-01 00:00:00
abstract：:In this study we examined if sleep time, caloric intake and energy expenditure are important contributors to development of ovariectomy-induced obesity in mice fed control or high fat diet (HFD). Twelve female mice at 6 weeks of age were divided into 2 groups: Sham (n = 5) and ovariectomized (OVX, n = 7). Mice were fe...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/j.lfs.2018.09.034
更新日期：2018-11-01 00:00:00
abstract：:Cordyceps sinensis (C. sinensis) is one of the well known fungi used in traditional Chinese medicine for treatment asthma and bronchial and lung inflammation. In this study, effects of C. sinensis methanolic extracts on bronchoalveolar lavage fluids (BALF) cells proliferation, inflammatory cytokines production, and ge...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/s0024-3205(00)01011-0
更新日期：2001-01-19 00:00:00
abstract：AIMS:Endoplasmic reticulum stress (ERS) as an emerging factor is involved in insulin resistance (IR), which is the pathological basis of diabetes mellitus. Accumulation of asymmetric dimethylarginine (ADMA), an endogenous inhibitor of nitric oxide synthase is associated with IR, but the underlying mechanisms have not b...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/j.lfs.2020.118373
更新日期：2020-11-01 00:00:00
abstract：:Nitric oxide (NO) is a key mediator in many physiological processes and one of the major receptors through which NO exerts its effects is soluble guanylyl cyclase. Guanylyl cyclase converts GTP to cyclic GMP as part of the cascade that results in physiological processes such as smooth muscle relaxation, neurotransmiss...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/s0024-3205(02)02361-5
更新日期：2003-01-17 00:00:00
abstract：:The colchicine-induced accumulation of vasopressin (AVP) and oxytocin (OXT) has recently been applied to estimate the synthesis and turnover rates for these neuropeptides in whole rat hypothalamus. In the present studies, this pharmacologic procedure has been examined as a potential method for estimating hypothalamic ...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/0024-3205(91)90441-d
更新日期：1991-01-01 00:00:00
abstract：:Several antihistamines were evaluated for their ability to interact with sigma, muscarinic and histaminic H1 binding sites in rat brain preparations. All of the antihistamines were able to interact with the sigma site, as well as the other two sites. In addition, tripelennamine was found to elicit sigma-like behaviors...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/0024-3205(90)90231-f
更新日期：1990-01-01 00:00:00
abstract：AIMS:Uric acid nephropathy (UAN) is due to excessive uric acid, which leads to hyperuricemia and kidney damage via the deposition of urate microcrystals in the kidneys. Iridoid glycosides of Paederia scandens (IGPS) is a major active component isolated from the traditional Chinese herb P. scandens (LOUR.) MERRILL (Rubi...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/j.lfs.2012.08.013
更新日期：2012-10-05 00:00:00
abstract：:An enzyme partially purified from bovine lung membranes appears to be endothelin converting enzyme (ECE). This enzyme specifically cleaves big endothelin-1 (big ET-1) at the proper site, between Trp21 and Val22, with maximum activity at pH 7.5 and with a Km of roughly 3 microM, to produce endothelin-1 (ET-1) and C-ter...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/0024-3205(92)90175-o
更新日期：1992-01-01 00:00:00
abstract：:Psoriasis is an inflammatory skin disease with preference for the skin and joints that occurs due to hyper-proliferation and abnormal apoptosis of keratinocytes. DUSP1 expression in dermal mesenchymal stem cells (MSCs) is obviously lower in psoriasis patients than that in healthy individuals. The present study aimed t...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/j.lfs.2019.03.018
更新日期：2019-04-15 00:00:00
abstract：AIMS:Gastric cancer (GC) is one of the most common malignant tumors in the world. Anti-angiogenic therapy is a useful strategy for the treatment of advanced GC. This study was aimed to systemically compare the anti-angiogenesis, anti-cancer efficacy, as well as the safety of four known anti-angiogenic drugs, namely ram...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/j.lfs.2020.117402
更新日期：2020-04-15 00:00:00
abstract：AIMS:Insulin receptor signaling in osteoblasts has been well established, but the effects of insulin on osteoclast proliferation are poorly explored. The objective of this study was to investigate the roles and the mechanisms of insulin on osteoclast proliferation. MAIN METHODS:After insulin treatment to primary osteo...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/j.lfs.2014.07.006
更新日期：2014-10-02 00:00:00
