Abstract：
:The role of serotonin (5-HT) in the regulation of steroidogenesis in the ovary via 5-HT2 receptor was studied in normal cycling rats and their preovulatory follicles. Oral administration of ketanserin (KET), a selective 5-HT2 antagonist, at 10 mg/kg BW at 1100h on the day of proestrus resulted in a marked decrease in serum estradiol levels immediately after the administration. 5-HT stimulated estradiol secretion by preovulatory follicles incubated for 5 hrs in Krebs-Ringer bicarbonate buffer. 5-HT (2.5 microM) -enhanced estradiol secretion was inhibited by KET (1-100 microM) in a dose-dependent manner. Secretion of progesterone and testosterone was also inhibited by about the same order of magnitude as estradiol. Addition of pregnenolone (1 microgram/ml) prevented the KET-inhibited progesterone production. Both testosterone and estradiol increased at the same ratio by the addition of progesterone (1 microgram/ml). The inhibition of secretion of these hormones was overcome by dbcAMP (0.1 mM). The present study suggests that 5-HT stimulates steroidogenesis in the metabolic pathway prior to pregnenolone in rat preovulatory follicles and the action of 5-HT could possibly be mediated by a 5-HT2 receptor.
journal_name
Life Scijournal_title
Life sciencesauthors
Tanaka E,Baba N,Toshida K,Suzuki Kdoi
10.1016/0024-3205(93)90713-dsubject
Has Abstractpub_date
1993-01-01 00:00:00pages
563-70issue
7eissn
0024-3205issn
1879-0631pii
0024-3205(93)90713-Djournal_volume
53pub_type
杂志文章相关文献
LIFE SCIENCES文献大全abstract：:The effects of the benzodiazepines diazepam and midazolam on the rigidity produced by systemic administration of morphine (15 mg/kg i.p.) were studied in rats. The rigidity was recorded as a tonic activity from the gastrocnemius-soleus muscle of non-anesthetized rats in the electromyogram. Both diazepam (1-5 mg/kg i.p...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/0024-3205(83)90612-4
更新日期：1983-01-01 00:00:00
abstract：:Rats with experimental allergic encephalomyelitis (EAE) induced with myelin or spinal cord show decreases in the content of sulphatides and cerebrosides and increases in the level of esterified cholesterol in the CNS. In this work it is shown that brain sulphatide changes can be obtained by injection of mixtures conta...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/0024-3205(82)90600-2
更新日期：1982-03-08 00:00:00
abstract：:The present report investigated whether percentages of circulating natural killer (NK) cells and NK cell activity (NKCA) are associated with psychological variables. Subjects (n = 95) were selected, based on a combination of low or high scores on questionnaires on daily hassles and self-reported symptoms, to create fo...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/0024-3205(93)90002-k
更新日期：1993-01-01 00:00:00
abstract：:A phencyclidine (PCP) receptor binding site has been solubilized in an active ligand-binding state from rat cerebral cortical membranes with sodium deoxycholate. Optimal receptor solubilization occurs at a detergent/protein ratio of 0.5 (w/w); for 5 mg protein/ml solubilized with 0.25% sodium deoxycholate, about 60% o...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/0024-3205(89)90567-5
更新日期：1989-01-01 00:00:00
abstract：:The anti-secretory activity of the competitive H2-antagonist HUK 978 was determined in rat, guinea-pig and dog. In all systems examined, HUK 978 was more potent than cimetidine and ranitidine both intravenously and orally. In addition, the compound at approximately equipotent doses as these established H2-antagonists ...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/0024-3205(85)90300-5
更新日期：1985-11-04 00:00:00
abstract：:The purpose of this present study was to develop a method for stimulation of acid secretion by the isolated perfused rat stomach. Rat stomachs were perfused in situ via the abdominal aorta and celiac axis with Krebs-Ringer bicarbonate buffer in the presence or absence of 10% ovine erythrocytes. The gastric lumen was p...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/0024-3205(84)90289-3
更新日期：1984-06-18 00:00:00
abstract：:The compound (+)-MR200 [(+)-methyl (1R,2S)-2-{[4-(4-chlorophenyl)-4-hydroxypiperidin-1-yl]methyl}-1-phenylcyclopropanecarboxylate] is a sigma ligand with increased affinity and selectivity compared to the structurally related ligand haloperidol. From the results of a previous study on the modulation of a systemically ...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/j.lfs.2005.10.005
更新日期：2006-04-18 00:00:00
abstract：:Increased oxidative stress and antioxidant deficit have been suggested to play a major role in adriamycin induced cardiomyopathy and congestive heart failure due to multiple treatments with adriamycin. In this study the cardio protective effect of Centella asiatica on myocardial marker enzymes and antioxidant enzymes ...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/j.lfs.2004.09.009
更新日期：2004-12-17 00:00:00
abstract：:Alterations in the cyclic AMP-dependent protein kinase activity ratio in response to putative neurotransmitters and other cyclic AMP-elevating agents in intact cerebral cortical slices and Krebs-Ringer particulate preparations from cerebral cortex were examined. Both norepinephrine (30 microM) and forskolin (20 microM...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/0024-3205(85)90629-0
更新日期：1985-07-08 00:00:00
abstract：:Male and female rats with two permanently indwelling intravenous catheters were infused for 2 hours with ovine prolactin. During equilibrium conditions the effects of intravenously injected L-DOPA and benzerazide (a blocker of dopa-decarboxylase) on steady state levels of ovine prolactin were measured. A dose of 4.5 m...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/0024-3205(82)90549-5
更新日期：1982-08-30 00:00:00
abstract：:We investigated whether nitric oxide (NO) exposure alters the balance between NO and endothelium-derived hyperpolarizing factor (EDHF) released from rat renal arteries. To produce states of acutely or chronically excessive NO, lipopolysaccharide (LPS) was administered intraperitoneally to rats in a single dose of 4 mg...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/j.lfs.2003.10.019
更新日期：2004-04-16 00:00:00
abstract：:Neonatally androgenized (NA) female rats were ovariectomized (OVX) as adults and given 1 mg of testosterone propionate/day for 7 days and the plasma prolactin (PRL) pattern compared with NA intact animals and normal OVX animals given estrogen or TP. NA intact animals had elevated basal (morning values) and an attenuat...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/0024-3205(82)90663-4
更新日期：1982-04-05 00:00:00
abstract：:Tiazofurin and retinoic acid synergistically induced differentiation and inhibited colony formation in HL-60 human promyelocytic leukemia cells in cell culture. The synergism was the result of different mechanisms of action, since the effect of tiazofurin, unlike that of retinoic acid, was prevented by addition of gua...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/0024-3205(90)90087-8
更新日期：1990-01-01 00:00:00
abstract：:Nitric oxide (NO) is a key mediator in many physiological processes and one of the major receptors through which NO exerts its effects is soluble guanylyl cyclase. Guanylyl cyclase converts GTP to cyclic GMP as part of the cascade that results in physiological processes such as smooth muscle relaxation, neurotransmiss...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/s0024-3205(02)02361-5
更新日期：2003-01-17 00:00:00
abstract：:We report that both Na+ and Ca2+ currents are involved in the action potentials and in the hormone release from rat somatotrophs in primary culture. Single somatotrophs were identified by reverse hemolytic plaque assay (RHPA) and transmembrane voltage and currents were recorded using the whole-cell mode of the patch-c...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/0024-3205(90)90021-i
更新日期：1990-01-01 00:00:00
abstract：:This paper describes the use of complex liposomes as real membrane models to evaluate the potential benefits of several antioxidants in relation to lipid peroxidation. The xanthine oxidase/Fe(3+)-ADP-EDTA and the Fe(2+)/H2O2 systems have been used to generate hydroxyl radicals and the water soluble azo-compound 2,2'-a...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/s0024-3205(03)00120-6
更新日期：2003-04-11 00:00:00
abstract：:The effect of oral administration of Clostridium butyricum Miyairi on the formation and dissolution of gallstones was examined in a mouse model of cholesterol cholelithiasis, in comparison with that of chenodeoxycholic acid (CDCA). A diet containing cholesterol and sodium cholate was used as a lithogenic diet. The fee...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/0024-3205(83)90149-2
更新日期：1983-01-31 00:00:00
abstract：:SARS-CoV-2 infection or COVID-19 has become a worldwide pandemic; however, effective treatment for COVID-19 remains to be established. Along with acute respiratory distress syndrome (ARDS), new and old cardiovascular injuries are important causes of significant morbidity and mortality in COVID-19. Exploring new approa...
journal_title：Life sciences
pub_type： 杂志文章,评审
doi：10.1016/j.lfs.2021.119130
更新日期：2021-01-26 00:00:00
abstract：:Ether à go-go (EAG) potassium channels possess oncogenic properties and have gained great interest as research tools for cancer detection and therapy. Besides, EAG electrophysiological properties are regulated through the cell cycle and determined by cytoskeletal interactions. Thus, because of the pivotal role of extr...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/j.lfs.2007.05.014
更新日期：2007-06-27 00:00:00
abstract：:Previous work has shown that hypomethylation of rRNA is an important control for protein synthesis in rat hepatocytes, and that the net hypomethylation appears to arise from cytoplasmic events. Here we show that demethylation of rRNA is catalyzed by microsomal preparations. The rRNA demethylation is dependent upon NAD...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/0024-3205(91)90283-h
更新日期：1991-01-01 00:00:00
abstract：:To understand whether chronic inflammation alters the development of morphine tolerance, the tail-flick test was used to evaluate the analgesic effect of morphine (75 mg tablet, s.c.) in the arthritic rats at the day 9-12 after the inoculation with Freund's adjuvant. Spinal cord monoamines and amino acid neurotransmit...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/s0024-3205(99)00008-9
更新日期：1999-01-01 00:00:00
abstract：:Histidine decarboxylase (HDC) activity and histamine content were measured in endoscopic gastric biopsy specimens of 19 control subjects with normogastrinemia and 6 patients with hypergastrinemia. In controls, the HDC activity was 3 fold higher in fundic mucosa (120 +/- 13 fmol/min/mg protein, mean +/- S.E.) than in a...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/0024-3205(91)90424-a
更新日期：1991-01-01 00:00:00
abstract：:P75(NTR) is a common neurotrophin receptor which binds all neurotrophins with similar affinities and has been shown to be capable of mediating programmed cell death. In this study, we investigated effects of the antipsychotic drugs (APDs) haloperidol, clozapine, quetiapine, and risperidone on p75(NTR) mRNA levels in P...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/j.lfs.2006.01.039
更新日期：2006-07-04 00:00:00
abstract：:The ontogeny of endothelin (ET) system in rats was studied in preterm (18 days of gestation), term (21 days of gestation) and 1 week post term rats. Brains were dissected out and (1) processed for the estimation of endogenous ET-1 by RIA and (2) membranes were prepared for radioreceptor binding. Receptor characteristi...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/0024-3205(92)90300-e
更新日期：1992-01-01 00:00:00
abstract：:1-Aminocyclopropane carboxylic acid (ACPC), a partial agonist of the glycine site on the NMDA receptor, prevents tolerance to the mu opioid morphine and the delta ligand [D-Pen2,D-Pen5]enkephalin (DPDPE) when co-administered with the opioid. In contrast, ACPC does not significantly influence tolerance to the kappa1 op...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/0024-3205(94)00753-5
更新日期：1994-01-01 00:00:00
abstract：:Plasma 7B2-immunoreactivity (7B2-IR) concentrations in umbilical artery (UA), umbilical vein (UV) and maternal vein (MV) were measured by RIA at the time of obstetrical delivery at term. Plasma 7B2-IR concentrations (Mean +/- SEM) in UA (N = 12), UV (N = 16) and MV (N = 16) were 725 +/- 69, 699 +/- 64 and 116 +/- 4.5 ...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/0024-3205(87)90744-2
更新日期：1987-10-19 00:00:00
abstract：:Nowadays, endocrine disrupting chemical pollution has become one of the major concerns due to the potential role of these chemicals in provoking endocrine disorders especially type 2 diabetes. As a widespread endocrine disrupting chemical, Bisphenol A, with modest estrogenic activity can exert its detrimental effects ...
journal_title：Life sciences
pub_type： 杂志文章,评审
doi：10.1016/j.lfs.2018.10.044
更新日期：2018-12-01 00:00:00
abstract：:The effect of Bacillus Calmette-Guérin (BCG) treatment in allergic pulmonary reaction was studied in mice genetically selected accordingly to a High (H-IVA) or Low (L-IVA) antibody responsiveness. Mice were immunized with ovalbumin (OVA) or OVA plus BCG. Two days after nasal antigenic challenge, seric IgE and IgG1 ant...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/j.lfs.2005.04.003
更新日期：2005-08-12 00:00:00
abstract：:We have examined the role of membrane hyperpolarization in mediating vascular hyporeactivity induced by bacterial lipopolysaccharide (LPS) in endothelial-denuded strips of rat thoracic aorta ex vivo. The injection of rats with LPS caused a significant fall of blood pressure and a severe vascular hyporeactivity to nore...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/s0024-3205(00)00978-4
更新日期：2000-12-29 00:00:00
abstract：:Hemi-pituitary glands of ovariectomized rats were superfused for 4 h with either LHRH or the analog buserelin (HOE 766) at several concentrations, and thereafter with medium only for another 1.5 h. In a further experiment glands were exposed for 2.5 h to LHRH or buserelin at a single concentration (5 ng/ml) and subseq...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/0024-3205(86)90524-2
更新日期：1986-08-04 00:00:00
