Abstract：
:Psoriasis is an inflammatory skin disease with preference for the skin and joints that occurs due to hyper-proliferation and abnormal apoptosis of keratinocytes. DUSP1 expression in dermal mesenchymal stem cells (MSCs) is obviously lower in psoriasis patients than that in healthy individuals. The present study aimed to explore the roles of DUSP1 in the proliferation and apoptosis of HaCaT cells treated with a cocktail of M5. We showed that DUSP1 was markedly reduced in psoriasis patients and M5-treated HaCaT cells compared with the control subjects. MTT and BrdU assays revealed that overexpression of DUSP1 significantly suppressed the proliferation of HaCaT cells. Furthermore, DUSP1 decreased M5-induced the upregulation of cyclin D1 and Rb. In addition, we demonstrated that forced overexpression of DUSP1 caused an augment of cell apoptosis rate, c-caspase 3 protein level and the Bax/Bcl-2 ratio. Finally, we determined that enhancing DUSP1 expression resulted in the reduction of p-ERK, p-Elk-1 and Egr-1 protein levels using western blot, and the Chromatin immunoprecipitation (ChIP) assay displayed that p-Elk-1 binds to the promoter of Egr-1 in HaCaT cells. The roles of DUSP1 in cell proliferation and apoptosis were abolished by overexpression of Egr-1. In summary, gain function of DUSP1 regulates proliferation and apoptosis of HaCaT cells through the ERK/Elk-1/Egr-1 signaling pathway.
journal_name
Life Scijournal_title
Life sciencesauthors
Yang J,Sun L,Han J,Zheng W,Peng Wdoi
10.1016/j.lfs.2019.03.018subject
Has Abstractpub_date
2019-04-15 00:00:00pages
47-53eissn
0024-3205issn
1879-0631pii
S0024-3205(19)30176-6journal_volume
223pub_type
杂志文章相关文献
LIFE SCIENCES文献大全abstract：:Peptide III-BTD has been recently identified as a non-selective nociceptin/orphanin FQ receptor ligand by screening of a synthetic peptide combinatorial library. In the present study we evaluated the pharmacological profile of peptide III-BTD in isolated tissues (mouse and rat vas deferens, guinea pig ileum) sensitive...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/s0024-3205(00)00931-0
更新日期：2000-12-01 00:00:00
abstract：:We have determined the effects of chronic exposure to the protein kinase C (PKC) activating drug 4-beta phorbol 12-myristate-13-acetate (PMA) on PKC isozymes and the rate of spontaneous contraction in neonatal rat cardiac myocytes in culture. Western blot analyses revealed that a two day exposure to 0.1-1 nM PMA incre...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/0024-3205(95)02048-n
更新日期：1995-01-01 00:00:00
abstract：:The purpose of this investigation was to determine the effects of experimental dicrocoeliosis on bile formation in the hamster. Studies were carried out at 120 days after infection with an oral dose of 40 metacercariae of Dicrocoelium dendriticum. A significant elevation in bile flow (+20%) and in the biliary output o...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/s0024-3205(98)00474-3
更新日期：1998-01-01 00:00:00
abstract：:Cardiovascular effects of the essential oil of Croton zehntneri (EOCZ) were investigated in conscious rats. In these preparations, intravenous (i.v.) injections of EOCZ (1-20 mg kg(-1)) and its main constituents anethole and estragole (both at 1-10 mg kg(-1)) elicited brief and dose-dependent hypotension and bradycard...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/j.lfs.2005.09.042
更新日期：2006-04-11 00:00:00
abstract：:Using a specific radioimmunoassay, we measured concentrations of plasma 7B2 (a novel pituitary polypeptide) immunoreactivity (7B2-IR) in normal human subjects, patients with chronic renal failure and those with liver cirrhosis. Mean (+/- SEM) values of plasma 7B2-IR in normal healthy men and women were 55.8 +/- 1.2 pg...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/0024-3205(87)90685-0
更新日期：1987-08-24 00:00:00
abstract：AIMS:The hypoxia-stimulated response of the endocrine system depends on the kind and duration of hypoxia. Hypoxia has been reported to stimulate testosterone (T) production in rats, but the mechanisms remain to be investigated. MATERIALS AND METHODS:Male rats were divided into two groups. The rats exposed to chronic i...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/j.lfs.2019.116694
更新日期：2019-09-15 00:00:00
abstract：:The effects of capsaicin on cellular growth and intracellular calcium mobilization were examined in human cervical carcinoma derivation, HeLa cells. Capsaicin inhibited cellular growth and increased intracellular calcium level in HeLa cells. This capsaicin-induced intracellular calcium concentration rise was blocked b...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/s0024-3205(99)00046-6
更新日期：1999-01-01 00:00:00
abstract：:N-acyl dehydroalanines have been shown to react with and scavenge oxygen derived free radicals. One of those compounds, the AD-5 (N-(paramethoxyphenylacetyl) dehydroalanine) has been examined for its ability to decrease the amount of reactive oxygen species which appeared when liver microsomes (isolated from rats pret...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/0024-3205(90)90106-2
更新日期：1990-01-01 00:00:00
abstract：:The modulation of Ca2+ release by synthetic bee venom melittin was examined in equine and human terminal cisternae-containing fractions. Melittin (0.1 microM) decreased the threshold of Ca(2+)-induced Ca2+ release by 20% in equine muscle and by 36% in human muscle. If terminal cisternae fractions were first preloaded ...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/0024-3205(92)90302-6
更新日期：1992-01-01 00:00:00
abstract：AIMS:Obesity is a complex health disorder and a trigger to many diseases like Diabetes mellitus (DM) and breast cancer (BrCa), both leading causes of morbidity and mortality worldwide. Also evidence demonstrates that abnormal glucose metabolism termed 'the Warburg effect' in cancer cell is closely associated with malig...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/j.lfs.2019.03.016
更新日期：2019-04-15 00:00:00
abstract：:Acetylcholine (ACh) may be an ovarian signaling molecule, since ACh is produced by non-neuronal granulosa cells (GCs) derived from the antral follicle, and likely also by their in vivo counterparts in the growing follicle. Furthermore, muscarinic ACh receptors (MR) are present in GC membranes and in cultured human GCs...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/j.lfs.2007.01.022
更新日期：2007-05-30 00:00:00
abstract：:It has been shown that some cytochrome P450-dependent enzyme activities could present daily fluctuations, particularly CYP3A isoenzymes which are enhanced during the dark period. The aim of this study was to investigate whether age and photoperiodic conditions at different circadian stages could influence these fluctu...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/s0024-3205(03)00271-6
更新日期：2003-06-06 00:00:00
abstract：:To determine the mechanism of anoxic vasoconstriction, precontracted rat aortic rings were exposed to 95% N2, which caused additional contraction. Reoxygenation (95% O2) resulted in initial relaxation followed by contraction. Indomethacin did not affect anoxic contraction or reoxygenation-mediated events, but NG-monom...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/0024-3205(91)90316-4
更新日期：1991-01-01 00:00:00
abstract：AIMS:Smokers have lower plasma concentrations of high-density lipoprotein (HDL) cholesterol and apolipoprotein A-I (apo A-I) compared with nonsmokers. To determine the molecular basis of this observation, the effect of activation of the aryl hydrocarbon receptor (AhR) on apo A-I gene expression was examined. MAIN METH...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/j.lfs.2012.06.002
更新日期：2012-07-26 00:00:00
abstract：:The effects of exposure to a schedule of unpredictable cold-immobilization stress on voluntary ethanol consumption were examined. Following testing for ethanol preference, rats were divided into high, medium and low ethanol consuming groups on the basis of daily ethanol intake (g/kg/day) and exposed to immobilization ...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/0024-3205(87)90580-7
更新日期：1987-03-30 00:00:00
abstract：OBJECTIVE:Metformin, introduced in 1957, is widely used as an anti-diabetic drug and has considerable benefits in cardiovascular disease reportedly, dependent or independent on its glucose-lowering effects. Aim of this study was to investigate the effect of metformin on gap junction and the inducibility of AF. METHODS...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/j.lfs.2020.117759
更新日期：2020-08-01 00:00:00
abstract：AIMS:The aim of this study is to investigate the expression and cytoprotective function of a 72-kDa heat shock protein (HSP72) using a reflux esophagitis model in rats. MAIN METHODS:Expression of HSP60, HSP72, and HSP90 in rat esophageal mucosa was evaluated by Western blot analysis before and after hyperthermia (42.5...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/j.lfs.2009.01.016
更新日期：2009-04-10 00:00:00
abstract：:Radio-binding assay, bioassay and HPLC detection were used to observe the antagonistic effects of dextrorphan on PCP's actions. Dextrorphan displayed high affinity to PCP receptor in the rabbit mesenteric blood vessels. It had weak PCP-like bioactivity, but could antagonize PCP's action dose-dependently in vitro study...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/0024-3205(87)90502-9
更新日期：1987-06-15 00:00:00
abstract：:Plasma opioid levels were determined in 9 obese non-diabetic subjects, their 8 age matched controls, and in 29 diabetic patients; 10 maintained on diet alone, 6 on an oral hypoglycemic agent (chlorpropamide) and 13 treated with insulin. Five age matched controls for the diabetic groups were also studied for comparison...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/0024-3205(84)90364-3
更新日期：1984-05-21 00:00:00
abstract：:The present work aims to document, in a previously described animal model of Parkinson (double bilateral striatal injection of 6-OHDA), the possible modifications of circadian markers rhythms i.e. temperature (T), heart rate (H) and locomotor activity (A) registered continuously by telemetry, in order to evaluate a po...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/s0024-3205(00)00751-7
更新日期：2000-08-18 00:00:00
abstract：:Reportedly, excitatory amino acids are involved in the control of gonadotropin secretion of rats and non-human primates. The aim of this study was to investigate the effect of chronic blockade of NMDA (N-methyl-D-aspartic acid) receptors by the non competitive receptor antagonist MK-801 on gonadotropin secretion and t...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/0024-3205(90)90218-g
更新日期：1990-01-01 00:00:00
abstract：:Since reduced levels of brain serotonin are known to cause behavioural abnormalities, to which diabetics are also prone, we investigated the effect, in rats, of chronic diabetes on brain serotonin concentration and on the numbers of 5-HT(1A) and 5-HT2 receptors in cerebral cortex and brainstem. Our data show that stre...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/s0024-3205(97)00084-2
更新日期：1997-01-01 00:00:00
abstract：:Studies were performed in the opossum to define the role of the L-arginine-nitric oxide (NO) pathway in lower esophageal sphincter (LES) relaxation to swallowing and vagal stimulation in viv and intramural nerve stimulation in vitro. In vivo, L-NAME, a water soluble NO synthase (NOS) inhibitor, caused antagonism of LE...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/0024-3205(92)90326-k
更新日期：1992-01-01 00:00:00
abstract：:Two recently isolated peptides, endomorphin-1 (Tyr-Pro-Trp-Phe-NH2) and endomorphin-2 (Tyr-Pro-Phe-Phe-NH2), are highly selective micro-opioid receptor agonists with analgesic actions in the tail-flick test. To further assess the analgesic properties of these peptides, the effects of endomorphin-1, endomorphin-2, and ...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/s0024-3205(00)00689-5
更新日期：2000-07-14 00:00:00
abstract：:The aim of this study was to investigate the effects of cyclosporin (CyA) treatment on biliary glutathione efflux in rats of different ages (1, 2, 4, and 24 months). CyA treatment reduced the liver content of total glutathione in 1-, 2- and 24 month old rats (-30%, -43% and -30%, respectively). By contrast, oxidized g...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/j.lfs.2003.06.019
更新日期：2003-11-14 00:00:00
abstract：AIMS:Data suggest pharmacological treatment of depression with selective serotonin reuptake inhibitors (SSRI) may impair bone health. Our group has previously modeled compromised craniofacial healing after treatment with sertraline, a commonly prescribed SSRI, and hypothesized potential culprits: alterations in bone ce...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/j.lfs.2020.117827
更新日期：2020-08-15 00:00:00
abstract：:The effects of melatonin treatment and pituitary transplants on serum total and free cholesterol levels, cholesterol esterification index, phospholipid levels and prolactin, thyroid-stimulating hormone (TSH), thyroxine (T4) and triiodothyronine (T3) levels were examined in rats. Male rats were grafted an anterior pitu...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/s0024-3205(97)00613-9
更新日期：1997-01-01 00:00:00
abstract：PURPOSE:Doxorubicin, as an effective chemotherapeutic drug, is commonly used for combating various solid and hematological tumors. However, doxorubicin-induced cardiotoxicity is considered as a serious adverse effect, and it limits the clinical use of this chemotherapeutic drug. The use of melatonin can lead to a decre...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/j.lfs.2019.117173
更新日期：2020-01-15 00:00:00
abstract：AIMS:We explored the effect of aerobic exercise on renal sinus adipose (RSA) accumulation and RSA accumulation-related renal injury in obese mice. MAIN METHODS:C57BL/6J male mice (n = 30) were evenly divided into three groups: control group (CON, n = 10), obese group (OB, n = 10; given high-fat diet) and obese + aerob...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/j.lfs.2021.119106
更新日期：2021-01-23 00:00:00
abstract：:Gloriosaols A-C, isolated from Yucca gloriosa (Agavaceae), are novel phenolic compounds structurally related to resveratrol. In the present study, we show that gloriosaols possess antiproliferative and pro-apoptotic activity on tumor cells of different histogenetic origin and that their cell growth inhibition potentia...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/j.lfs.2007.07.010
更新日期：2007-08-23 00:00:00
