Abstract:
:We have determined the effects of chronic exposure to the protein kinase C (PKC) activating drug 4-beta phorbol 12-myristate-13-acetate (PMA) on PKC isozymes and the rate of spontaneous contraction in neonatal rat cardiac myocytes in culture. Western blot analyses revealed that a two day exposure to 0.1-1 nM PMA increased the total amount of delta PKC, whereas, 100 nM PMA concentrations caused a complete down-regulation of the alpha PKC and an 80 kDa zeta PKC-like protein. In addition, 100 nM PMA treatment for 2 days did not result in complete down-regulation of the beta, delta, and epsilon PKC isozymes in Western blot and immunocytochemical studies. We also found a PKC-mediated enhancement of the rate of contraction in these cells following prolonged exposure to PMA (1-100nM). Our studies suggest that this enhancement of contraction rate may be mediated by the beta, delta, or epsilon PKC isozymes. A better understanding of the role(s) of PKC isozymes in the modulation of cardiac functions may reveal selective targets for therapies of cardiac disorders.
journal_name
Life Scijournal_title
Life sciencesauthors
Johnson JA,Adak S,Mochly-Rosen Ddoi
10.1016/0024-3205(95)02048-nsubject
Has Abstractpub_date
1995-01-01 00:00:00pages
1027-38issue
11eissn
0024-3205issn
1879-0631pii
002432059502048Njournal_volume
57pub_type
杂志文章相关文献
LIFE SCIENCES文献大全abstract::Intracerebroventricular (i.c.v.) administration of the neurohypophyseal neuropeptide arginine8-vasopressin (AVP) results in a dose-dependent attenuation of endotoxin-induced fever (EIF) in rats. Specific antagonists of the neuropeptided(CH2)5[Tyr(Me)2]AVP for V1 receptors, d(CH2)5[dlle2lle4]AVP for the V2 receptors an...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/0024-3205(92)90448-x
更新日期:1992-01-01 00:00:00
abstract:AIMS:Vitamin D is a well-known endocrine regulator of calcium/phosphate homeostasis and has been reported as having a wide range of activities that are potentially beneficial for human health. This study aimed to investigate the effects of pretreatment of vitamin D3 (100, 1000, and 10,000 IU/kg) against lipopolysacchar...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/j.lfs.2020.117703
更新日期:2020-07-15 00:00:00
abstract::Activated hepatic stellate cells (HSC; lipocytes; Ito cells) proliferate and are responsible for extracellular matrix synthesis during hepatic fibrogenesis. During activation, HSC undergo transdifferentiation into myofibroblasts expressing alpha-smooth muscle actin (alpha-SMA). Adenosine 3', 5'-cyclic monophosphate (c...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/s0024-3205(99)00157-5
更新日期:1999-01-01 00:00:00
abstract:AIMS:Oxidative stress has been largely implicated in the pathogenesis of anoxia/reoxygenation injury. Ganoderma atrum polysaccharide (PSG-1), the most abundant component extracted from the fruiting bodies of G. atrum, has been shown to possess potent antioxidant activity. In this study, we investigated whether PSG-1 at...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/j.lfs.2009.09.001
更新日期:2009-10-21 00:00:00
abstract::Diazepam binding inhibitor (DBI) is a 9-kD polypeptide that was first isolated in 1983 from rat brain by monitoring its ability to displace diazepam from the benzodiazepine (BZD) recognition site located on the extracellular domain of the type A receptor for gamma-aminobutyric acid (GABAA receptor) and from the mitoch...
journal_title:Life sciences
pub_type: 杂志文章,评审
doi:10.1016/0024-3205(91)90440-m
更新日期:1991-01-01 00:00:00
abstract::Maintenance of the cellular calcium homeostasis plays an important role for neuronal cell function and interneuronal cell to cell communication. Therefore, alterations of the neuronal Ca2+ homeostasis may play a crucial role for brain aging in general and for age-related deficits in cognitive functions particularly. N...
journal_title:Life sciences
pub_type: 杂志文章,评审
doi:10.1016/0024-3205(94)00381-5
更新日期:1994-01-01 00:00:00
abstract::Muscarinic receptors are expressed in smooth muscle throughout the body. In most instances, the muscarinic receptor population in smooth muscle is composed of mainly the M2 and M3 subtypes in an 80% to 20% mixture. The M3 subtype mediates phosphoinositide hydrolysis and calcium mobilization, whereas the M2 subtype med...
journal_title:Life sciences
pub_type: 杂志文章,评审
doi:10.1016/s0024-3205(97)00433-5
更新日期:1997-01-01 00:00:00
abstract:AIMS:Thrombin is a serine proteinase that is not only involved in coagulation cascade, but also mediates a number of biological responses relevant to tissues repair, and induces bronchoconstriction. TGF-β plays a pivotal role in airway remodeling due to its effects on airway smooth muscle proliferation and extracellula...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/j.lfs.2018.02.012
更新日期:2018-03-15 00:00:00
abstract::Over the past few years, it has been reported that physical and psychological stress elevate plasma interleukin-6 (IL-6), and that neural cells can produce IL-6 and have receptors for IL-6 (IL-6R). However, it is unknown whether IL-6 plays a role in regulating the functions of neural cells in response to stress. We de...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/s0024-3205(98)00212-4
更新日期:1998-01-01 00:00:00
abstract:AIMS:Liver fibrosis is a difficult problem in the medical field. We previously reported that curcumol, a bioactive substance, may inhibit the pathological angiogenesis of liver sinusoidal endothelial cells (LSECs) and play a good anti-hepatic fibrosis effect. However, the mechanism of curcumol inhibiting angiogenesis i...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/j.lfs.2020.118696
更新日期:2021-01-01 00:00:00
abstract::The gastric mucosa is frequently exposed to different exogenous and endogenous ulcerative agents. Alcoholism is one of the risk factors for the development of mucosal damage in the stomach. This study aimed to assess if a probiotic strain Lactobacillus rhamnosus GG (LGG) is capable of protecting the gastric mucosa fro...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/j.lfs.2007.03.018
更新日期:2007-05-16 00:00:00
abstract::Respiratory performance, heart rate and blood pressure were studied in halothane anesthetized rats after administration of taurine and the putative taurine antagonist 6-aminomethyl-3-methyl-4H-1,2,4-benzothiadiazine-1, 1-dioxide hydrochloride (TAG). Intracerebroventricular (i.c.v.) taurine depressed ventilation due to...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/0024-3205(83)90720-8
更新日期:1983-10-24 00:00:00
abstract::Preliminary data suggest that amygdalar corticotropin-releasing factor (CRF) is regulated by nicotinic agonists. We sought to confirm and extend these observations by determining the effects of various concentrations of nicotine on CRF messenger RNA expression in the AR-5 immortalized amygdalar cell line. Nicotine pro...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/s0024-3205(99)00539-1
更新日期:1999-01-01 00:00:00
abstract::The temperature effects of naloxone and naltrexone (1-30 mg/kg) were examined in well habituated male rats. These drugs had a similar time course and potency, producing a dose-dependent hypothermia followed several hours later by a hyperthermia. A subsequent study found that not only did 30 mg/kg of naloxone or naltre...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/0024-3205(87)90563-7
更新日期:1987-03-16 00:00:00
abstract::The effects of denervation of alpha 2 adrenoceptor binding sites were examined in canine arteries and veins. Denervation of the lower abdominal aorta, renal and femoral arteries and femoral veins marked reduced vessel norepinephrine concentrations. Denervation had little effect on the concentration of alpha 2 adrenoce...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/s0024-3205(83)80005-8
更新日期:1983-07-25 00:00:00
abstract::Asp-Thr-Met-Arg-Cys-Met-Val-Gly-Arg-Val-Tyr-Arg-Pro-Cys-Trp-Glu-Val, melanin concentrating hormone (MCH), is a cyclic hormone possessing both MCH-like (melanin granule aggregating effect) and melanocyte stimulating hormone (MSH)-like (melanin granule dispersing effect) activities. Nine ring-contracted analogues were s...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/0024-3205(89)90460-8
更新日期:1989-01-01 00:00:00
abstract::In the present study, we investigated the effects of calmodulin, adenosine 5'-triphosphate (ATP) and pertussis toxin (PT) on phorbol ester (PMA) (a protein kinase C activator) induced inhibition of ANF-stimulated cyclic GMP formation in cells from the human renal cell line, SK-NEP-1. PMA inhibited ANF-stimulated guany...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/0024-3205(91)90508-9
更新日期:1991-01-01 00:00:00
abstract::Nitric oxide (NO) plays a critical role in the pathogenesis of endotoxin-induced uveitis (EIU). Since NO is a labile free radical, it is difficult to examine the dynamics of NO directly in vivo. In this study, we established a system for direct monitoring of the dynamics of NO and partial pressure of oxygen (pO(2)) in...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/j.lfs.2007.01.021
更新日期:2007-03-27 00:00:00
abstract::The potential effect of maternal caffeine ingestion upon total brain protein and the concentration of two prototype neuropeptides, thyrotropin-releasing hormone (TRH) and its derivative, cyclo (His-Pro) in neonates was examined during the nursing period in the context of variable maternal protein intake. Maternal caff...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/0024-3205(83)90332-6
更新日期:1983-11-21 00:00:00
abstract::Type-II iodothyronine 5'-deiodinase activity (5'-D) in both anterior pituitary and pineal was significantly elevated at 2400 h, approximately 0.5- and 20-fold higher than the noon value, respectively. The nocturnal rise in both organs was abolished by 6 h additional light. Short-term constant darkness did not alter 5'...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/0024-3205(88)90027-6
更新日期:1988-01-01 00:00:00
abstract::Activin A is a member of the transforming growth factor-beta superfamily that exerts its diverse biological effects through bindings to activin specific transmembrane serine/threonine kinase receptors. The fibroblast-mediated contraction of a collagen gel is thought to be a model of part of the wound-repair response a...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/s0024-3205(00)00480-x
更新日期:2000-03-01 00:00:00
abstract::Kinin release and its involvement in inflammatory exudation were assessed by immuno-blot analysis of the kinin-precursor protein, high molecular weight kininogen (HK). HK consists of heavy (H) chain, bradykinin and light (L) chain. After bradykinin was released by plasma kallikrein, HK remains two-chain-kinin-free for...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/0024-3205(93)90206-i
更新日期:1993-01-01 00:00:00
abstract::The effects of exposure to a schedule of unpredictable cold-immobilization stress on voluntary ethanol consumption were examined. Following testing for ethanol preference, rats were divided into high, medium and low ethanol consuming groups on the basis of daily ethanol intake (g/kg/day) and exposed to immobilization ...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/0024-3205(87)90580-7
更新日期:1987-03-30 00:00:00
abstract::Intravenous injection of prostacyclin (100 micrograms/kg) in rats resulted in a decrease of systolic blood pressure within 2 minutes. Concentrations of cAMP in 15 brain regions and nuclei were determined by radioimmunoassay. In lower brain stem nuclei, such as the nucleus of the solitary tract and the lateral reticula...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/0024-3205(87)90439-5
更新日期:1987-12-07 00:00:00
abstract:AIMS:To evaluate potential agents of therapeutic value in tissue inflammation, we studied norcantharidin (NCTD) and its derivatives for their effects on immune responses of human peripheral blood mononuclear cells (PBMC) in vitro. MAIN METHODS:PBMC proliferation was evaluated by tritiated thymidine uptake method. The ...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/j.lfs.2008.11.020
更新日期:2009-02-13 00:00:00
abstract::NMDA receptors are glutamate-regulated ion channels of critical importance for many neurophysiological and neuropathological processes. Mg2+ blocks the NMDA receptor by binding to the channel pore with an apparent affinity that depends on the membrane potential. We have investigated the effect of NMDA and the required...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/s0024-3205(01)00975-4
更新日期:2001-03-09 00:00:00
abstract::The pure estrogen antagonist ICI-182780, at doses above 50 micrograms/kg, effectively inhibited the initiation of embryo implantation in rats when administered on day 4 of pregnancy (day 1 = sperm positive). The same dose inhibited the implantation initiating effect of intravenous 25 ng of estradiol-17 beta in delayed...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/0024-3205(93)90186-7
更新日期:1993-01-01 00:00:00
abstract::Alzheimer's disease (AD) involves selective loss of muscarinic m2, but not m1, subtype neuroreceptors in the posterior parietal cortex of the human brain. Emission tomographic study of the loss of m2 receptors in AD is limited by the fact that there is currently no available m2-selective radioligand which can penetrat...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/0024-3205(93)90161-u
更新日期:1993-01-01 00:00:00
abstract:AIMS:Ischemia/reperfusion injury (IRI), resulting from hypoxic damage within a graft, is the leading cause of cell death and graft rejection. In this study, we investigated whether a HIF-1α inhibitor or various antioxidants were able to prevent ischemic injury in a cellular model in which experimental hypoxia was induc...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/j.lfs.2015.02.004
更新日期:2015-04-01 00:00:00
abstract::A growing number of studies reported that rosiglitazone (a PPARgamma agonist) could ameliorate the painful state and prevent stress-induced depression. However, whether rosiglitazone can prevent pain-induced depression is unclear. This study aimed to explore the antidepressant effects of rosiglitazone in L5 spinal ner...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/j.lfs.2018.04.057
更新日期:2018-06-15 00:00:00