Prolonged phorbol ester treatment down-regulates protein kinase C isozymes and increases contraction rate in neonatal cardiac myocytes.


:We have determined the effects of chronic exposure to the protein kinase C (PKC) activating drug 4-beta phorbol 12-myristate-13-acetate (PMA) on PKC isozymes and the rate of spontaneous contraction in neonatal rat cardiac myocytes in culture. Western blot analyses revealed that a two day exposure to 0.1-1 nM PMA increased the total amount of delta PKC, whereas, 100 nM PMA concentrations caused a complete down-regulation of the alpha PKC and an 80 kDa zeta PKC-like protein. In addition, 100 nM PMA treatment for 2 days did not result in complete down-regulation of the beta, delta, and epsilon PKC isozymes in Western blot and immunocytochemical studies. We also found a PKC-mediated enhancement of the rate of contraction in these cells following prolonged exposure to PMA (1-100nM). Our studies suggest that this enhancement of contraction rate may be mediated by the beta, delta, or epsilon PKC isozymes. A better understanding of the role(s) of PKC isozymes in the modulation of cardiac functions may reveal selective targets for therapies of cardiac disorders.


Life Sci


Life sciences


Johnson JA,Adak S,Mochly-Rosen D




Has Abstract


1995-01-01 00:00:00














  • Luteolin attenuates high glucose-induced podocyte injury via suppressing NLRP3 inflammasome pathway.

    abstract:AIMS:Diabetic nephropathy is a growing health concern, which is reported to be associated with inflammation. Luteolin has been explored for the treatment of some diabetic complications. Although several studies have verified the effect of luteolin on diabetic nephropathy, the mechanism by which the therapeutic effects ...

    journal_title:Life sciences

    pub_type: 杂志文章


    authors: Yu Q,Zhang M,Qian L,Wen D,Wu G

    更新日期:2019-05-15 00:00:00

  • Tonic pain response in mice: effects of sex, season and time of day.

    abstract::Seasonal and diurnal variations in tonic pain reactions were examined in female and male CBA/J mice maintained in a 12/12 dark/light cycle, at controlled temperature and humidity conditions. Animals were injected into the dorsum of one hindpaw with a dilute (20 microl, 1%) formalin solution. Pain-related behaviors wer...

    journal_title:Life sciences

    pub_type: 杂志文章


    authors: Perissin L,Facchin P,Porro CA

    更新日期:2003-01-10 00:00:00

  • Flavonoid modulation of protein kinase C activation.

    abstract::The flavonoid quercetin exhibited a biphasic effect on calcium and phospholipid-dependent protein kinase (protein kinase C) activity from rat brain and pig thyroid. At a low concentration (10(-7) M) quercetin stimulated the enzyme activity whereas at higher concentrations quercetin was inhibitory. By contrast the synt...

    journal_title:Life sciences

    pub_type: 杂志文章


    authors: Picq M,Dubois M,Munari-Silem Y,Prigent AF,Pacheco H

    更新日期:1989-01-01 00:00:00

  • Augmented analgesic effects of L-tryptophan combined with low current transcranial electrostimulation.

    abstract::The analgesic effects of low current transcranial electrostimulation are both naloxone and pCPA-reversible, suggesting that they may be mediated in part by endogenous opioid and serotonergic activity. The present experiments indicate that pretreatment with the serotonin precursor L-tryptophan results in an increased a...

    journal_title:Life sciences

    pub_type: 杂志文章


    authors: Malin DH,Lake JR,Hamilton RF,Skolnick MH

    更新日期:1990-01-01 00:00:00

  • Analgesic effects of intrathecally-administered 3 alpha-hydroxy-5 alpha-pregnan-20-one in a rat mechanical visceral pain model.

    abstract::Recent studies in animals have demonstrated that the steroid, 3 alpha-hydroxy-5 alpha-pregnan-20-one (3A5P), is a potent analgesic when given intracerebroventricularly. Several studies in humans report that spinal steroids are effective in the treatment of chronic low-back pain when given in combination with morphine....

    journal_title:Life sciences

    pub_type: 杂志文章


    authors: Winfree CJ,Coombs DW,DeLeo JA,Colburn RW

    更新日期:1992-01-01 00:00:00

  • The opposing effects of calmodulin, adenosine 5'-triphosphate, and pertussis toxin on phorbol ester induced inhibition of atrial natriuretic factor stimulated guanylate cyclase in SK-NEP-1 cells.

    abstract::In the present study, we investigated the effects of calmodulin, adenosine 5'-triphosphate (ATP) and pertussis toxin (PT) on phorbol ester (PMA) (a protein kinase C activator) induced inhibition of ANF-stimulated cyclic GMP formation in cells from the human renal cell line, SK-NEP-1. PMA inhibited ANF-stimulated guany...

    journal_title:Life sciences

    pub_type: 杂志文章


    authors: Sekiya M,Frohlich ED,Cole FE

    更新日期:1991-01-01 00:00:00

  • Increased endogenous nitric oxide production induced by physical exercise in peripheral arterial occlusive disease patients.

    abstract::The effects of 14-day physical exercise or iloprost treatment (0.5-2 ng/Kg/min) on endogenous nitric oxide production and neutrophil adhesion were evaluated in 20 patients with peripheral arterial occlusive disease (Fontaine Stage II). Peripheral venous blood samples and 4-h urine samples were collected before, immedi...

    journal_title:Life sciences

    pub_type: 临床试验,杂志文章,随机对照试验


    authors: Arosio E,Cuzzolin L,De Marchi S,Minuz P,Degan M,Crivellente F,Zannoni M,Benoni G

    更新日期:1999-01-01 00:00:00

  • Inhibition by nitric oxide of Ca(2+) responses in rat pancreatic alpha-cells.

    abstract::This study examined the effect of nitric oxide (NO) on the cytosolic free Ca(2+) concentration ([Ca(2+)](c)) of alpha-cells isolated from rat pancreatic islets. When extracellular glucose was reduced from 7 to 0 mM, about half of the alpha-cells displayed [Ca(2+)](c) oscillations. Nicardipine, a Ca(2+) channel blocker...

    journal_title:Life sciences

    pub_type: 杂志文章


    authors: Sugino F,Ishikawa T,Nakada S,Kaneko Y,Yamamoto Y,Nakayama K

    更新日期:2002-05-24 00:00:00

  • Inhibitory effect of naringin on lipopolysaccharide (LPS)-induced endotoxin shock in mice and nitric oxide production in RAW 264.7 macrophages.

    abstract::Lipopolysaccharide (LPS) has been known to induce endotoxin shock via production of inflammatory modulators such as tumor necrosis factor alpha (TNF-alpha), or nitric oxide (NO). In this study, we have examined the effect of naringin (NG), one of the flavonoids, on LPS-induced endotoxin shock in mice and NO production...

    journal_title:Life sciences

    pub_type: 杂志文章


    authors: Kanno S,Shouji A,Tomizawa A,Hiura T,Osanai Y,Ujibe M,Obara Y,Nakahata N,Ishikawa M

    更新日期:2006-01-11 00:00:00

  • Prostate tumor CXC-chemokine profile correlates with cell adhesion to endothelium and extracellular matrix.

    abstract::Though chemokines of the CXC family are thought to play key roles in neoplastic transformation and tumor invasion, information about CXC chemokines in prostate cancer is sparse. To evaluate the involvement of CXC chemokines in prostate cancer, we analyzed the CXC coding mRNA of both chemokine ligands (CXCL) and chemok...

    journal_title:Life sciences

    pub_type: 杂志文章


    authors: Engl T,Relja B,Blumenberg C,Müller I,Ringel EM,Beecken WD,Jonas D,Blaheta RA

    更新日期:2006-03-13 00:00:00

  • Highly-sensitive identification of alpha-fetoprotein mRNA in circulating peripheral blood of hepatocellular carcinoma patients.

    abstract::In order to capture hepatocellular carcinoma (HCC) cells in circulating peripheral blood, we made analysis to see if alpha-fetoprotein (AFP) mRNA exists in the peripheral blood obtained from patients with HCC and also, as a control, from hepatitis-viral-marker-positive patients without HCC and a healthy volunteer. As ...

    journal_title:Life sciences

    pub_type: 杂志文章


    authors: Funaki N,Tanaka J,Seto S,Kasamatsu T,Kaido T,Imamura M

    更新日期:1995-01-01 00:00:00

  • Deficits in D-fenfluramine-sensitive pool of brain 5-HT following withdrawal from chronic cocaine exposure.

    abstract::Recent head-twitch response (HTR) studies in mice have indicated that withdrawal from chronic cocaine exposure produces deficits in CNS conversion of L-tryptophan to 5-HT. In the present study, the ability of 5-HT releaser, d-fenfluramine, was utilized to induce the HTR in mice following abstinence from chronic cocain...

    journal_title:Life sciences

    pub_type: 杂志文章


    authors: Darmani NA

    更新日期:1997-01-01 00:00:00

  • Drug targeting of leptin resistance.

    abstract::Leptin regulates glucose, lipid and energy homeostasis as well as feeding behavior, serving as a bridge between peripheral metabolically active tissues and the central nervous system (CNS). Indeed, this adipocyte-derived hormone, whose circulating levels mirror fat mass, not only exerts its anti-obesity effects mainly...

    journal_title:Life sciences

    pub_type: 杂志文章,评审


    authors: Santoro A,Mattace Raso G,Meli R

    更新日期:2015-11-01 00:00:00

  • MMP-9 antisense oligodeoxynucleotide exerts an inhibitory effect on osteoclastic bone resorption by suppressing cell migration.

    abstract::We have previously shown that matrix metalloproteinases (MMPs) play a role in osteoclastic bone resorption by facilitating migration of osteoclastic cells toward bone surface through matrices. Of MMPs identified so far, MMP-9 is likely the most important proteinase for the action, since osteoclasts express this enzyme...

    journal_title:Life sciences

    pub_type: 杂志文章


    authors: Ishibashi O,Niwa S,Kadoyama K,Inui T

    更新日期:2006-09-20 00:00:00

  • Influence of in vitro and in vivo insulin treatment on the hormone (histamine, serotonin, endorphin and triiodothyronine) content of thymus and spleen cells.

    abstract::Thymic and spleen cells were treated in vitro or in vivo with insulin. The in vitro treatments were done with 10(-6), 10(-9), 10(-12) and 10(-15) M concentrations for 30 min and after that histamine, serotonin, endorphin and triiodothyronine (T3) content of the cells were detected by using antibodies to the hormones a...

    journal_title:Life sciences

    pub_type: 杂志文章


    authors: Csaba G,Kovács P,Pállinger E

    更新日期:2006-02-02 00:00:00

  • Dopamine receptor mRNAs in the rat lymphocytes.

    abstract::It has been suggested that dopamine might play a role in the regulation of the immune system. In this study, reverse transcriptase-polymerase chain reaction (RT-PCR) was used to investigate the expression of mRNA for the different subtypes of dopamine receptors in the rat lymphocytes. D1, D3 and D5 receptor mRNAs were...

    journal_title:Life sciences

    pub_type: 杂志文章


    authors: Caronti B,Calderaro C,Passarelli F,Palladini G,Pontieri FE

    更新日期:1998-01-01 00:00:00

  • Amygdalin delays cell cycle progression and blocks growth of prostate cancer cells in vitro.

    abstract:AIMS:Despite impressive survival benefits from new agents to treat metastasized prostate cancer (PCa), progressive drug resistance hinders long-term response and restricts the efficacy of subsequent therapy. Due to reported antitumor activity of amygdalin and growing popularity for complementary and alternative medicin...

    journal_title:Life sciences

    pub_type: 杂志文章


    authors: Makarević J,Tsaur I,Juengel E,Borgmann H,Nelson K,Thomas C,Bartsch G,Haferkamp A,Blaheta RA

    更新日期:2016-02-15 00:00:00

  • L-tryptophan attenuation of the dopaminergic and behavioral responses to cocaine.

    abstract::This study assessed the effects of acute intravenous L-tryptophan (neutral amino acid precursor for serotonin) administration on cocaine-induced dopaminergic responses. Male Sprague-Dawley rats were surgically implanted with guide cannulas in the nucleus accumbens 5 days prior to the study and with vascular catheters ...

    journal_title:Life sciences

    pub_type: 杂志文章


    authors: Molina PE,Ahmed N,Gatley J,Volkow ND,Abumrad NN

    更新日期:2001-09-07 00:00:00

  • Icariin protects neurons from endoplasmic reticulum stress-induced apoptosis after OGD/R injury via suppressing IRE1α-XBP1 signaling pathway.

    abstract::Icariin (ICA), a flavonol glycoside isolated from Epimedium, has been considered as a potential alternative therapy for ischemic stroke. However, the protective mechanisms of ICA on cerebral ischemia-reperfusion (I/R) are not fully illuminated yet. The effects of ICA on ER stress and inflammatory response which were i...

    journal_title:Life sciences

    pub_type: 杂志文章


    authors: Mo ZT,Liao YL,Zheng J,Li WN

    更新日期:2020-08-15 00:00:00

  • Sumoylation enhances the activity of the TGF-β/SMAD and HIF-1 signaling pathways in keloids.

    abstract::Excessive fibrosis and extracellular matrix deposition resulting from upregulation of target genes expression mediated by transforming growth factor-beta (TGF-β)/SMAD and hypoxia inducible factor-1 (HIF-1) signaling pathways are the main mechanisms that drive keloid formation. Sumoylation is a protein posttranslationa...

    journal_title:Life sciences

    pub_type: 杂志文章


    authors: Lin X,Wang Y,Jiang Y,Xu M,Pang Q,Sun J,Yu Y,Shen Z,Lei R,Xu J

    更新日期:2020-08-15 00:00:00

  • Identification of 14-hydroxy-retro-retinol and 4-hydroxy-retinol as endogenous retinoids in rats throughout neonatal development.

    abstract::14-Hydroxy-retro-retinol was previously described as an in vivo and in vitro metabolite of retinol. Furthermore, the retinoid 4-hydroxy-retinol was identified as an endogenous occurring retinoid in the amphibian organism and an in vitro metabolite of retinol. We describe in the present study that 14-hydroxy-retro-reti...

    journal_title:Life sciences

    pub_type: 杂志文章


    authors: Rühl R,Hamscher G,Garcia AL,Nau H,Schweigert FJ

    更新日期:2005-02-18 00:00:00

  • Thiorphan potentiation of stress-induced analgesia in the mouse.

    abstract::Experiments were done to examine the analgesic effect of thiorphan alone or in combination with stress in mice. Analgesia was assessed by measuring jump latencies from a 55 degrees C hot plate. Thiorphan exhibited weak analgesic properties evidenced by significant increases in jump latencies only after 300 mg/kg i.p. ...

    journal_title:Life sciences

    pub_type: 杂志文章


    authors: Greenberg R,O'Keefe EH

    更新日期:1982-09-20 00:00:00

  • Dual targeting of solid lipid nanoparticles grafted with 83-14 MAb and anti-EGF receptor for malignant brain tumor therapy.

    abstract:AIMS:Solid lipid nanoparticles (SLNs) conjugated with surface 83-14 monoclonal antibody (8314MAb) and anti-epithelial growth factor receptor (AEGFR) were synthesized and applied to permeate the blood-brain barrier (BBB) and inhibit the growth of glioblastoma multiforme (GBM). MAIN METHODS:8314MAb and AEGFR were crossl...

    journal_title:Life sciences

    pub_type: 杂志文章


    authors: Kuo YC,Lee CH

    更新日期:2016-02-01 00:00:00

  • Co-administration of dextromethorphan during pregnancy and throughout lactation significantly decreases the adverse effects associated with chronic morphine administration in rat offspring.

    abstract::In this study, we have focused our investigation of the facts whether co-administration of a NMDA antagonist dextromethorphan (DM) with morphine during pregnancy and throughout lactation could prevent the adverse effects associated with chronic morphine administration in rat offspring. Adult female Sprague-Dawley rats...

    journal_title:Life sciences

    pub_type: 杂志文章


    authors: Tao PL,Yeh GC,Su CH,Wu YH

    更新日期:2001-10-05 00:00:00

  • Ethanol and caffeine consumption modulates the expression of miRNAs in the cerebellum and plasma of UChB rats.

    abstract:AIMS:The present study aimed to verify changes in cerebellar and plasmatic expression of miRNAs after the chronic consumption of ethanol and caffeine in the UChB rat, an experimental model for alcoholism. MATERIAL AND METHODS:Male rats at 5 months of age, were divided into the following groups (n = 10/group): 1. Ethan...

    journal_title:Life sciences

    pub_type: 杂志文章


    authors: Rossetto IMU,Cagnon VHA,Lizarte FSN,Tirapelli LF,Tirapelli DPC,Arantes RMS,Chuffa LGA,Martinez FE,Martinez M

    更新日期:2019-07-15 00:00:00

  • The effects of verapamil and ethanol on body temperature and motor coordination.

    abstract::Male, adult mice of the Binghamton heterogeneous stock received one of two doses of ethanol (1.0 g/kg or 2.0 g/kg in saline) alone or in combination with the calcium (Ca2+) slow channel blocker, verapamil (5.45 mg/kg in 25% v/v ethanol in saline). Hypothermic responses and motor incoordination were assessed in terms o...

    journal_title:Life sciences

    pub_type: 杂志文章


    authors: Johnston JE,Draski LJ,Molina JC,Burright RG,Reynoso G,Calendrillo BA,Isaacson RL

    更新日期:1986-12-01 00:00:00

  • Role of phospholamban in NO/EDRF-induced relaxation in rat aorta.

    abstract::The role of endothelium-derived nitric oxide (NO) to cause smooth muscle phospholamban (PLB) phosphorylation was studied in the isolated perfused rat aorta precontracted with norepinephrine using a back-phosphorylation technique. NO-induced relaxation was associated with increased PLB-phosphorylation while norepinephr...

    journal_title:Life sciences

    pub_type: 杂志文章


    authors: Karczewski P,Kelm M,Hartmann M,Schrader J

    更新日期:1992-01-01 00:00:00

  • The anti-inflammatory effects of quinolinic acid in the rat.

    abstract::Quinolinic acid (QUIN) levels are elevated in patients and animals suffering from chronic infectious diseases. In the present study, male Sprague-Dawley rats were used to test the anti-inflammatory effects of QUIN using the carrageenan (CGN)-induced paw edema assay and the CGN sponge assay. Results of these studies in...

    journal_title:Life sciences

    pub_type: 杂志文章


    authors: Heyliger SO,Mazzio EA,Soliman KF

    更新日期:1999-01-01 00:00:00

  • Towards mimicking natural protein channels with aligned carbon nanotube membranes for active drug delivery.

    abstract:AIMS:Carbon nanotube (CNT) membranes offer an exciting opportunity to mimic natural protein channels due to 1) a mechanism of dramatically enhanced fluid flow 2) ability to place 'gatekeeper' chemistry at the entrance to pores 3) the ability for biochemical reactions to occur on gatekeeper molecules and 4) an ability t...

    journal_title:Life sciences

    pub_type: 杂志文章,评审


    authors: Majumder M,Stinchcomb A,Hinds BJ

    更新日期:2010-04-10 00:00:00

  • Is highly expressed ACE 2 in pregnant women "a curse" in times of COVID-19 pandemic?

    abstract::Angiotensin-converting enzyme 2 (ACE 2) is a membrane-bound enzyme that cleaves angiotensin II (Ang II) into angiotensin (1-7). It also serves as an important binding site for SARS-CoV-2, thereby, facilitating viral entry into target host cells. ACE 2 is abundantly present in the intestine, kidney, heart, lungs, and f...

    journal_title:Life sciences

    pub_type: 杂志文章,评审


    authors: Dhaundiyal A,Kumari P,Jawalekar SS,Chauhan G,Kalra S,Navik U

    更新日期:2021-01-01 00:00:00