Effect of the opioid methionine enkephalinamide on signal transduction in human T-lymphocytes.

Abstract:

:T cell receptor (TCR/CD3) induced fluctuations in intracellular free ionizied calcium, [Ca2+]i, was analysed in the human T leukemia cell clone, Jurkat, cultured in the presence of the opioid methionine enkephalinamide (Met-Enk) in titrated concentrations (10[-7] to 10[-15] M) or saline (PBS). In the majority of individual experiments, the activation-induced fluctuations in [Ca2+]i were similar in cells cultured in the presence of Met-Enk and PBS, respectively. However, when all the experimental data from 101 separate TCR/CD3-activation experiments with Met-Enk were compared with the 67 separate control experiments, we found that a fraction (20-40%) of the individual sets of Met-Enk experiments responded significantly different when compared to PBS-controls. In this fraction of experiments the increase in [Ca2+]i after ligation of the TCR/CD3 complex was extremely slow compared to controls. Moreover, the levels of [Ca2+]i in this particular fraction were lower than control levels prior to ligation of the TCR/CD3 complex. The data support the idea that signal transduction in T cells can be influenced by endogenous opioid. The data therefore give credit to the evolving hypothesis of a functional relationship between the neuroendocrine system and the immune system.

journal_name

Life Sci

journal_title

Life sciences

authors

Sørensen AN,Claësson MH

doi

10.1016/s0024-3205(98)00055-1

subject

Has Abstract

pub_date

1998-01-01 00:00:00

pages

1251-9

issue

14

eissn

0024-3205

issn

1879-0631

pii

S0024320598000551

journal_volume

62

pub_type

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