Abstract:
BACKGROUND AND OBJECTIVE:Severe acute respiratory distress syndrome coronavirus-2 (SARS-CoV-2), the causative agent of coronavirus disease 2019 (COVID-19), is a member of the coronavirus family. Coronavirus infections in humans are typically associated with respiratory illnesses; however, viral RNA has been isolated in serum from infected patients. Coronaviruses have been identified as a potential low-risk threat to blood safety. The Mirasol Pathogen Reduction Technology (PRT) System utilizes riboflavin and ultraviolet (UV) light to render blood-borne pathogens noninfectious, while maintaining blood product quality. Here, we report on the efficacy of riboflavin and UV light against the pandemic virus SARS-CoV-2 when tested in both plasma and platelets units. MATERIALS AND METHODS:Stock SARS-CoV-2 was grown in Vero cells and inoculated into either plasma or platelet units. Those units were then treated with riboflavin and UV light. The infectious titres of SARS-CoV-2 were determined by plaque assay using Vero cells. A total of five (n = 5) plasma and three (n = 3) platelet products were evaluated in this study. RESULTS:In both experiments, the measured titre of SARS-CoV-2 was below the limit of detection following treatment with riboflavin and UV light. The mean log reductions in the viral titres were ≥3·40 and ≥4·53 for the plasma units and platelet units, respectively. CONCLUSION:Riboflavin and UV light effectively reduced the titre of SARS-CoV-2 in both plasma and platelet products to below the limit of detection in tissue culture. The data suggest that the process would be effective in reducing the theoretical risk of transfusion transmitted SARS-CoV-2.
journal_name
Vox Sangjournal_title
Vox sanguinisauthors
Keil SD,Ragan I,Yonemura S,Hartson L,Dart NK,Bowen Rdoi
10.1111/vox.12937subject
Has Abstractpub_date
2020-08-01 00:00:00pages
495-501issue
6eissn
0042-9007issn
1423-0410journal_volume
115pub_type
杂志文章相关文献
VOX SANGUINIS文献大全abstract:BACKGROUND AND OBJECTIVES:Platelet concentrates are frequently transfused to patients with reduced immunity. An exhaustive description of their viral content is needed to prevent unwanted infection. MATERIAL AND METHODS:To track viral sequences, a shotgun metagenomics approach was used on a bank of 300 platelets conce...
journal_title:Vox sanguinis
pub_type: 杂志文章
doi:10.1111/vox.12695
更新日期:2018-07-18 00:00:00
abstract::A method is described for the production of mouse monoclonal antibodies to HBsAg. 4 clones produced antibodies directed at the a and one at the d determinant of HBsAg. These antibodies were conjugated to horse radish peroxidase and used in an Elisa for the detection of HBsAg in donated blood. Antibody C10 produced con...
journal_title:Vox sanguinis
pub_type: 杂志文章
doi:10.1111/j.1423-0410.1984.tb00070.x
更新日期:1984-01-01 00:00:00
abstract::In a 2-year study involving 133 premature low birth weight (less than 1,500 g) infants, the impact of CMV infection via blood transfusion was assessed. 8.4% (7 out of 83) of transfused infants and 10% (7 out of 70) of those exposed to seropositive blood acquired CMV. In those less than 1,250 g the infection rate rose ...
journal_title:Vox sanguinis
pub_type: 杂志文章
doi:10.1111/j.1423-0410.1992.tb01199.x
更新日期:1992-01-01 00:00:00
abstract::Anti-HBc is the most sensitive indicator of current or recent hepatitis B virus infection, hence its detection is valuable in laboratory diagnosis of the infection. Dane particles isolated from the plasma of a single chronic carrier and his autologous IgG labeled with 125I were utilized in a solid-phase sandwich radio...
journal_title:Vox sanguinis
pub_type: 杂志文章
doi:10.1111/j.1423-0410.1977.tb02259.x
更新日期:1977-12-01 00:00:00
abstract::K12 is a high-prevalence antigen, with no known antithetical partner, that is associated with the Kell blood group system. We report studies on the 5th propositus (MS) with the K:-12 phenotype and the second case to show that the K12 is inherited. The anti-K12 in his serum did not destroy antigen-positive incompatible...
journal_title:Vox sanguinis
pub_type: 杂志文章
doi:10.1111/j.1423-0410.1995.tb02543.x
更新日期:1995-01-01 00:00:00
abstract:OBJECTIVES:Reconstitution of hematopoiesis by means of peripheral blood stem cells is a valid alternative to autologous bone marrow transplantation. The aim of this investigation was to increase the efficiency of collection of circulating blood progenitor cells and to obtain a purer product for transplant. METHODS:We ...
journal_title:Vox sanguinis
pub_type: 临床试验,杂志文章
doi:10.1046/j.1423-0410.1997.7340230.x
更新日期:1997-01-01 00:00:00
abstract:OBJECTIVES:We have developed and optimised a new flow-cytometric method for the measurement of the Fc function of intravenous immunoglobulin (IVIg) preparations, which is important in predicting the effector function of immunoglobulin (Ig) in such preparations. MATERIALS AND METHODS:Ig was bound to a monocytic cell li...
journal_title:Vox sanguinis
pub_type: 杂志文章
doi:10.1159/000031178
更新日期:2000-01-01 00:00:00
abstract:BACKGROUND AND OBJECTIVES:The aim of this study was to assess potentially relevant clinical characteristics which influence the decision to transfuse red cells in critically ill patients with low haemoglobin concentrations (6.0-10.0 g/dl). MATERIALS AND METHODS:This was a retrospective observational cohort study of pa...
journal_title:Vox sanguinis
pub_type: 杂志文章
doi:10.1111/vox.12831
更新日期:2019-11-01 00:00:00
abstract::Prevention of post-transfusion malaria remains a worrying problem. Until now it was very difficult to obtain homologous antigens, and immunologic methods used to detect malaria were expensive. We tested the ready-to-use Plasmodium falciparum prepared slides using the IFA (indirect fluorescent antibody) test on 866 sel...
journal_title:Vox sanguinis
pub_type: 杂志文章
doi:10.1111/j.1423-0410.1983.tb01933.x
更新日期:1983-01-01 00:00:00
abstract::Platelet concentrates (PC) prepared from pooled buffy coat (BC-PC) contain a variable number of leukocytes from different donors. We questioned whether storage of BC-PC can lead to a lymphocyte activation in the sense of a mixed lymphocyte reaction. BC-PC were prepared from four ABO-identical buffy coats and we undert...
journal_title:Vox sanguinis
pub_type: 杂志文章
doi:10.1111/j.1423-0410.1995.tb00345.x
更新日期:1995-01-01 00:00:00
abstract::We tested serum samples from 11 HIV-seropositive and 32 HIV-seronegative Italian patients with hemophilia A or B for the presence of lymphocytotoxic antibodies (LCTAs). No patients had the acquired immune deficiency syndrome and all had received over many years heat-untreated commercial clotting factors. LCTAs directe...
journal_title:Vox sanguinis
pub_type: 杂志文章
doi:10.1111/j.1423-0410.1988.tb01612.x
更新日期:1988-01-01 00:00:00
abstract::2 patients on the drug azapropazone developed positive antiglobulin tests. Their sera were each found to contain two distinct antibodies. One was an IgG antibody, probably a drug-dependent autoantibody of the alpha-methyldopa type, but could have been related to the disease. The other was certainly a drug-dependent an...
journal_title:Vox sanguinis
pub_type: 杂志文章
doi:10.1111/j.1423-0410.1984.tb00095.x
更新日期:1984-01-01 00:00:00
abstract:OBJECTIVE:To evaluate the incidence and severity of and risk factors for thrombocytopenia at birth in neonates with red cell alloimmunization. STUDY DESIGN:All neonates with haemolytic disease of the foetus/newborn (HDFN) due to red cell alloimmunization admitted to our centre between January 2000 and September 2010 w...
journal_title:Vox sanguinis
pub_type: 杂志文章,随机对照试验
doi:10.1111/j.1423-0410.2011.01539.x
更新日期:2012-04-01 00:00:00
abstract::An alternative to blood transfusion, based on oxygen-carrying solutions, has been sought for over a century. The present 'first-generation' haemoglobin-products were based on observations that crosslinking with, for example, glutaraldehyde, overcame subunit dissociation and renal toxicity. Experience with these soluti...
journal_title:Vox sanguinis
pub_type: 杂志文章,评审
doi:10.1111/j.1423-0410.2006.00789.x
更新日期:2006-08-01 00:00:00
abstract::We studied 684 sera obtained from 20 hemophilia patients with AIDS/AIDS-related complex (ARC), 89 asymptomatic HIV+, 76 HIV- hemophilia patients and 151 healthy controls for antibodies against recombinant CD4 (rCD4). Twenty-two percent of AIDS/ARC patients, 10% of asymptomatic HIV+ patients, 17% of HIV-patients, and 1...
journal_title:Vox sanguinis
pub_type: 杂志文章
doi:10.1111/j.1423-0410.1992.tb01165.x
更新日期:1992-01-01 00:00:00
abstract:BACKGROUND AND OBJECTIVES:Donor plasmapheresis involves the removal of a weight-adjusted volume of plasma and the return of cellular components to the donor. Although plasma volume generally returns to normal, some residual effect on vital signs may be possible. This analysis was performed to determine the possible eff...
journal_title:Vox sanguinis
pub_type: 杂志文章
doi:10.1111/vox.12188
更新日期:2015-01-01 00:00:00
abstract::The mechanism of CD4+ lymphocyte depletion, which is the main immunological feature in HIV-infected patients, is unclear. We investigated whether gp120-immunoglobulin-complement complexes on the surface of CD4+ cells might be involved in the elimination of CD4+ lymphocytes. The results obtained in 63 HIV-infected pati...
journal_title:Vox sanguinis
pub_type: 杂志文章
doi:10.1111/j.1423-0410.1993.tb02511.x
更新日期:1993-01-01 00:00:00
abstract::Little is known about the patient's reasons for participating in an autologous blood (AUB) collection program. We surveyed 110 AUB donors in our hospital-based collection program. Although fear of infection from an allogeneic blood transfusion was cited by 20% of AUB donors, 68.2% indicated that their physician's reco...
journal_title:Vox sanguinis
pub_type: 杂志文章
doi:10.1111/j.1423-0410.1995.tb01679.x
更新日期:1995-01-01 00:00:00
abstract:BACKGROUND:The amino acid substitutions caused by ABO gene variants are usually predicted to impact the glycosyltransferase function. Here, the effect of an amino acid substitution in the vicinity of the catalytic active region of the B-glycosyltransferase was explored in vitro and in silico study, which is important f...
journal_title:Vox sanguinis
pub_type: 杂志文章
doi:10.1111/vox.13041
更新日期:2020-12-16 00:00:00
abstract::The decline in the incidence of TAH from 1985-1990 was considerable and based on a variety of interventions that led to increasingly intensive scrutiny of potential donors. The decline in incidence observed since 1990, and particularly since 1992, has been truly extraordinary and based principally on the introduction ...
journal_title:Vox sanguinis
pub_type: 杂志文章,评审
doi:10.1111/j.1423-0410.1994.tb04539.x
更新日期:1994-01-01 00:00:00
abstract::Forty-six subjects (44 HIV antibody-positive) with some degree of immune deficiency (at least TH/TS ratio below 1) were randomly distributed into 4 treatment groups. Each group was assigned to 1 of 4 products to be used exclusively for a 1-year period: 1 concentrate was of intermediate purity and not heat-treated, and...
journal_title:Vox sanguinis
pub_type: 临床试验,杂志文章,随机对照试验
doi:10.1111/j.1423-0410.1987.tb04911.x
更新日期:1987-01-01 00:00:00
abstract::2,140 pregnant Sudanese women attending the antenatal clinic in Omdurman Maternity Hospital during the period November 1975 to December 1976 had ABO and Rh typing performed. Their sera were also screened by a modified papain enzyme technique for the presence of atypical antibodies. The rate of Rhesus immunization, the...
journal_title:Vox sanguinis
pub_type: 杂志文章
doi:10.1111/j.1423-0410.1978.tb02889.x
更新日期:1978-01-01 00:00:00
abstract::Human IgG separated by Cohn fractionation showed variability in the content of aggregates, plasminogen and anticomplement activity. The plasminogen was removed or markedly reduced by affinity chromatography on Sepharose-lysine. Anticomplement activity was reduced by chromatography of Cohn fraction II on DEAE-cellulose...
journal_title:Vox sanguinis
pub_type: 杂志文章
doi:10.1111/j.1423-0410.1977.tb00619.x
更新日期:1977-01-01 00:00:00
abstract::The use of granulocyte transfusions in profoundly neutropenic patients has increased markedly in recent years. Whenever a pulmonary infiltrate develops during the course of these transfusions, the question arises as to what role the transfusions are playing and whether the transfusions should be discontinued to preven...
journal_title:Vox sanguinis
pub_type: 杂志文章
doi:
更新日期:1982-02-01 00:00:00
abstract:BACKGROUND AND OBJECTIVES:Blood transfusions can transmit parasitic infections, such as those caused by Plasmodium (malaria), Trypanosoma cruzi (Chagas' disease), and Babesia (babesiosis). A higher degree of blood transfusion safety would be reached if methods were available for inactivating such parasites. MATERIALS ...
journal_title:Vox sanguinis
pub_type: 杂志文章
doi:10.1046/j.1423-0410.1997.7240211.x
更新日期:1997-01-01 00:00:00
abstract:BACKGROUND AND OBJECTIVES:Despite long being a mainstay in describing platelet activation via degranulation, interlaboratory variation remains an issue in measurement of membrane CD62P by flow cytometry. Our objective was to identify actions that may minimize this variation. MATERIALS AND METHODS:Sixteen laboratories ...
journal_title:Vox sanguinis
pub_type: 临床试验,杂志文章,多中心研究
doi:10.1111/vox.12023
更新日期:2013-07-01 00:00:00
abstract::Prolonged storage of red blood cells (RBCs) at 4 degrees C results in decreased intracellular ATP levels with diminished posttransfusion survival. Meryman described a preservative medium, exceptional in its capacity to increase these intracellular levels during the first weeks of storage and later in maintaining adequ...
journal_title:Vox sanguinis
pub_type: 杂志文章
doi:10.1111/j.1423-0410.1994.tb00327.x
更新日期:1994-01-01 00:00:00
abstract:BACKGROUND:The topology of the blood supply chain network can take different forms in different settings, depending on geography, politics, costs, etc. Many developed countries are moving towards centralized networks. The goal for all blood distribution networks, regardless of topology, remains the same: to satisfy dem...
journal_title:Vox sanguinis
pub_type: 杂志文章
doi:10.1111/vox.12706
更新日期:2018-11-01 00:00:00
abstract::A Bombay, Le(a-b-) saliva was shown to lack Pneumococcus type XIV activity, an unusual situation, since this sample should be rich in this precursor to the ABO blood group substances. However, the sample was found to contain a new serological specificity, Le-c. It is argued that simple Mendelian inheritance does not o...
journal_title:Vox sanguinis
pub_type: 杂志文章
doi:10.1111/j.1423-0410.1975.tb00508.x
更新日期:1975-01-01 00:00:00
abstract:BACKGROUND AND OBJECTIVES:Leishmaniasis is a disease caused by protozoan parasites of the genus Leishmania, which are intracellular parasites of monocytes and macrophages. Transmission of the organism has been observed by transfusion of infected blood from asymptomatic donors to immunocompromised recipients, leading to...
journal_title:Vox sanguinis
pub_type: 杂志文章
doi:10.1111/j.1423-0410.2006.00810.x
更新日期:2006-08-01 00:00:00