Photoinactivation of Leishmania donovani infantum in red cell suspensions by a flexible thiopyrylium sensitizer.

Abstract:

BACKGROUND AND OBJECTIVES:Leishmaniasis is a disease caused by protozoan parasites of the genus Leishmania, which are intracellular parasites of monocytes and macrophages. Transmission of the organism has been observed by transfusion of infected blood from asymptomatic donors to immunocompromised recipients, leading to clinically apparent disease. There is no licensed Leishmania screening test currently available. MATERIALS AND METHODS:This study investigated the potential for a novel DNA-intercalating photosensitizer, thiopyrylium (TP), to inactivate Leishmania donovani infantum in red cell (RBC) suspensions. RESULTS:A 5.7 TCID50 reduction of Leishmania was observed in samples treated with 12.5 micromole l(-1) TP and 1.1 J cm(-2) light. CONCLUSIONS:Leishmania is highly sensitive to photoinactivation under conditions that have been previously demonstrated to maintain RBC properties during 42 days of storage.

journal_name

Vox Sang

journal_title

Vox sanguinis

authors

Wagner SJ,Skripchenko A,Salata J,Cardo LJ

doi

10.1111/j.1423-0410.2006.00810.x

subject

Has Abstract

pub_date

2006-08-01 00:00:00

pages

178-80

issue

2

eissn

0042-9007

issn

1423-0410

pii

VOX810

journal_volume

91

pub_type

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