Standardization of CD62P measurement: results of an international comparative study.

Abstract:

BACKGROUND AND OBJECTIVES:Despite long being a mainstay in describing platelet activation via degranulation, interlaboratory variation remains an issue in measurement of membrane CD62P by flow cytometry. Our objective was to identify actions that may minimize this variation. MATERIALS AND METHODS:Sixteen laboratories participated in an international comparative study. Two sets of platelet samples were prepared in one laboratory. Set 1 was stained and fixed; set 2 was fixed and required staining at participating laboratories. A single-staining method was used, and platelet populations were selected based on forward scatter/side scatter characteristics. Calibration beads were used to standardize measurement across different instruments. RESULTS:There was a large discrepancy in reported CD62P values among study sites [interlaboratory coefficient of variance (CV): 36-78%]. When electronic data were re-analysed by a single analyst using a consistent gating strategy and a stable reference point, variation decreased markedly (CV < 12%), indicating a problem with isotype control samples, possibly related to sample fixation or shipment. CONCLUSION:Consensus regarding gating strategies and use of a reliable reference point would greatly improve agreement in interlaboratory CD62P measurement.

journal_name

Vox Sang

journal_title

Vox sanguinis

authors

Levin E,Serrano K,Devine DV,Biomedical Excellence for Safer Transfusion (BEST) Collaborative.

doi

10.1111/vox.12023

subject

Has Abstract

pub_date

2013-07-01 00:00:00

pages

38-46

issue

1

eissn

0042-9007

issn

1423-0410

journal_volume

105

pub_type

临床试验,杂志文章,多中心研究
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    authors: Kiefel V,Santoso S,Katzmann B,Mueller-Eckhardt C

    更新日期:1988-01-01 00:00:00

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    pub_type: 杂志文章

    doi:

    authors: Le Pendu J,Lemieux RU,Dalix AM,Lambert F,Oriol R

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    journal_title:Vox sanguinis

    pub_type: 杂志文章

    doi:10.1111/j.1423-0410.1993.tb05157.x

    authors: Yamamoto F,McNeill PD,Yamamoto M,Hakomori S,Harris T

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    journal_title:Vox sanguinis

    pub_type: 杂志文章

    doi:10.1111/j.1423-0410.1986.tb02008.x

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  • The ABCB6 mutation p.Arg192Trp is a recessive mutation causing the Lan- blood type.

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  • Transmission of parasites by blood transfusion.

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    journal_title:Vox sanguinis

    pub_type: 杂志文章,评审

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    更新日期:1998-01-01 00:00:00

  • Non-HLA lymphocytotoxic antibodies in HIV-seropositive and HIV-seronegative hemophiliacs.

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    journal_title:Vox sanguinis

    pub_type: 杂志文章

    doi:10.1111/j.1423-0410.1988.tb01612.x

    authors: Conte R,Giovanardi L,Tazzari PL,De Rosa V,Rodorigo G,Re MC

    更新日期:1988-01-01 00:00:00

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    journal_title:Vox sanguinis

    pub_type: 杂志文章

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    journal_title:Vox sanguinis

    pub_type: 杂志文章

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    pub_type: 杂志文章

    doi:10.1111/vox.12312

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  • Screening plasma donors for high-titre antibody to cytomegalovirus using a latex agglutination test.

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    journal_title:Vox sanguinis

    pub_type: 杂志文章

    doi:10.1111/j.1423-0410.1990.tb05014.x

    authors: McDonald C,Barbara JA,al-Izzi A,Contreras M

    更新日期:1990-01-01 00:00:00

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    journal_title:Vox sanguinis

    pub_type: 杂志文章,评审

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    pub_type: 杂志文章

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    doi:

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    journal_title:Vox sanguinis

    pub_type: 杂志文章

    doi:10.1111/j.1423-0410.1975.tb00508.x

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  • Pulmonary complications during granulocyte transfusions: incidence and clinical features.

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    doi:

    authors: Karp DD,Ervin TJ,Tuttle S,Gorgone BC,Lavin P,Yunis EJ

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    pub_type: 杂志文章

    doi:10.1111/j.1423-0410.1980.tb02367.x

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