Detection of early anti-p24 HIV responses in EIA- and immunoblot-negative individuals. Implications for confirmatory testing.

Abstract:

:A sensitive and specific radioimmunoprecipitation assay was developed for the detection and analysis of anti-HIV antibody response in human sera with the use of 125I-labelled purified HIV proteins with subsequent sodium-dodecylsulfate gel electrophoresis (125I-RIPA). The 125I-RIPA was shown to be as specific but at least 1 log more sensitive with respect to the detection of gp41env and p24gag than the immunoblot analysis as tested in serum samples from several risk groups. Sequential sera were obtained from 9 individuals who seroconverted for HIV antibodies. In 4 individuals, antibody to p24gag was detected in earlier serum samples by the 125I-RIPA than by EIA or immunoblot; in the other 5 individuals, the detection of p24gag concorded in enzyme-linked immunosorbent assay (EIA), immunoblot and 125I-RIPA. Moreover, in one of 78 randomly chosen EIA-negative sera from individuals at high risk, antibodies to p24gag could be detected by the 125I-RIPA. This early seroconversion was confirmed 3 months later by means of immunoblotting and EIA. The specificity of the 125I-RIPA was further demonstrated by analyzing sequential EIA-negative serum samples from 10 individuals at risk for AIDS, collected during 2 years at 3-monthly intervals. All 80 serum samples were found to be negative in the 125I-RIPA and the individuals revealed no signs of HIV infection. The 125I-RIPA technique may be a valuable confirmatory assay in the serology of HIV infections. The sensitivity of this test provides a reliable measure of effective sensitivity when new-generation screening tests are evaluated.(ABSTRACT TRUNCATED AT 250 WORDS)

journal_name

Vox Sang

journal_title

Vox sanguinis

authors

Huisman JG,Winkel IN,Lelie PN,Tersmette M,Goudsmit J,Miedema F

doi

10.1111/j.1423-0410.1987.tb04910.x

subject

Has Abstract

pub_date

1987-01-01 00:00:00

pages

31-6

issue

1

eissn

0042-9007

issn

1423-0410

journal_volume

53

pub_type

杂志文章
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    abstract:BACKGROUND:The Joint Commission lists improving staff communication (handoffs) as part of several National Safety Goals. In this study, we developed an electronic web-based charting system for clinical pathology handoffs, which primarily consist of transfusion medicine calls, and evaluated the advantages over a paper-b...

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    pub_type: 杂志文章

    doi:10.1111/vox.12913

    authors: Mohammad MK,Wooten MS,Maier CL,Hill CE,Guarner J,Roback JD,Winkler AM,Sullivan HC

    更新日期:2020-07-01 00:00:00

  • Pst I polymorphism of the antithrombin III gene in a French population.

    abstract::Pst I endonuclease fragment length polymorphism of the antithrombin III probe was studied in a French population. Among 25 subjects tested, 15 were found heterozygous at the Pst I site, so that this polymorphism might serve as a useful markers of antithrombin III gene in families with antithrombin III deficiency. ...

    journal_title:Vox sanguinis

    pub_type: 杂志文章

    doi:10.1111/j.1423-0410.1985.tb00787.x

    authors: Le Paslier D,Rochu D,Lucotte G

    更新日期:1985-01-01 00:00:00

  • Prolonged storage of red cells: the effect of pH, adenine and phosphate.

    abstract::Prolonged storage of red blood cells (RBCs) at 4 degrees C results in decreased intracellular ATP levels with diminished posttransfusion survival. Meryman described a preservative medium, exceptional in its capacity to increase these intracellular levels during the first weeks of storage and later in maintaining adequ...

    journal_title:Vox sanguinis

    pub_type: 杂志文章

    doi:10.1111/j.1423-0410.1994.tb00327.x

    authors: Mazor D,Dvilansky A,Meyerstein N

    更新日期:1994-01-01 00:00:00

  • Clinical consequences of the extremely rare anti-PP1Pk isoantibodies in pregnancy: a case series and review of the literature.

    abstract:BACKGROUND:The absence of the red cell antigens P, P1 and Pk , known as 'p', represents an extremely rare red cell phenotype. Individuals with this phenotype spontaneously form anti-PP1Pk isoantibodies, associated with severe haemolytic transfusion reactions, recurrent spontaneous abortion and haemolytic disease of the...

    journal_title:Vox sanguinis

    pub_type: 杂志文章

    doi:10.1111/vox.13042

    authors: Di Ciaccio P,Cutts B,Alahakoon TI,Dennington PM,Soo LA,Curnow J

    更新日期:2020-12-16 00:00:00

  • Efficiency of leukocyte removal by filters made of superfine glass fiber membranes.

    abstract:BACKGROUND AND OBJECTIVES:To demonstrate the application of leukocyte removal filters made of a new type of filter material - superfine glass fiber - for depleting leukocytes in SAGM red cell suspensions and preventing nonhemolytic transfusion reactions. MATERIALS AND METHODS:The extent of leukocyte depletion and red ...

    journal_title:Vox sanguinis

    pub_type: 杂志文章

    doi:10.1159/000031015

    authors: Zou Y,Sun Q,Li A,Yao F,Hu Z,Li Z L,Ma W

    更新日期:1999-01-01 00:00:00

  • The strategies to reduce iron deficiency in blood donors randomized trial: design, enrolment and early retention.

    abstract:BACKGROUND AND OBJECTIVES:Repeated blood donation produces iron deficiency. Changes in dietary iron intake do not prevent donation-induced iron deficiency. Prolonging the interdonation interval or using oral iron supplements can mitigate donation-induced iron deficiency. The most effective operational methods for reduc...

    journal_title:Vox sanguinis

    pub_type: 临床试验,杂志文章,随机对照试验

    doi:10.1111/vox.12210

    authors: Bialkowski W,Bryant BJ,Schlumpf KS,Wright DJ,Birch R,Kiss JE,D'Andrea P,Cable RG,Spencer BR,Vij V,Mast AE

    更新日期:2015-02-01 00:00:00

  • A family demonstrating inheritance of the Leach phenotype: a Gerbich-negative phenotype associated with elliptocytosis.

    abstract::The proposita of a Canadian family and 2 of her sibs have the rare Leach phenotype and provide the first evidence that the Leach phenotype is an inherited condition. Their red cells are Ge: -2, -3 and fail to react, by the antiglobulin test, with the monoclonal antibodies GERO, BRIC 4 and BRIC 10. The minor sialoglyco...

    journal_title:Vox sanguinis

    pub_type: 杂志文章

    doi:10.1111/j.1423-0410.1986.tb04860.x

    authors: Daniels GL,Shaw MA,Judson PA,Reid ME,Anstee DJ,Colpitts P,Cornwall S,Moore BP,Lee S

    更新日期:1986-01-01 00:00:00

  • Photoinactivation of Leishmania donovani infantum in red cell suspensions by a flexible thiopyrylium sensitizer.

    abstract:BACKGROUND AND OBJECTIVES:Leishmaniasis is a disease caused by protozoan parasites of the genus Leishmania, which are intracellular parasites of monocytes and macrophages. Transmission of the organism has been observed by transfusion of infected blood from asymptomatic donors to immunocompromised recipients, leading to...

    journal_title:Vox sanguinis

    pub_type: 杂志文章

    doi:10.1111/j.1423-0410.2006.00810.x

    authors: Wagner SJ,Skripchenko A,Salata J,Cardo LJ

    更新日期:2006-08-01 00:00:00

  • A new method to collect granulocytes using a low dose of hydroxyethyl starch.

    abstract::A new granulocyte collection method employing discontinuous flow centrifugation and low donor dose of hydroxyethyl starch (HES) is presented. Following the procedure, the increase in serum polysaccharide concentration was 100 mg/dl, about 20% of that seen after standard discontinuous flow leukapheresis. The average ef...

    journal_title:Vox sanguinis

    pub_type: 杂志文章

    doi:10.1111/j.1423-0410.1983.tb04111.x

    authors: Szymanski IO

    更新日期:1983-01-01 00:00:00

  • Accumulation of soluble inflammatory mediators between blood donation and pre-storage leucocyte depletion.

    abstract:BACKGROUND AND OBJECTIVES:Leucocyte-derived cytokines accumulate in stored whole blood. Pre-storage leucocyte depletion has reduced cytokine levels and, consequently, febrile non-haemolytic transfusion reactions. As leucocyte filtration and component separation can be performed until 24 h after donation, we hypothesize...

    journal_title:Vox sanguinis

    pub_type: 杂志文章

    doi:10.1111/j.1423-0410.2008.01132.x

    authors: Chudziak D,Sireis W,Pfeiffer HU,Henschler R,Seifried E,Bönig H

    更新日期:2009-02-01 00:00:00

  • Use of synthetic antigens with the carbohydrate structure of asialoglycophorin A for the specification of Thomsen-Friedenreich antibodies.

    abstract::The panagglutination phenomenon described by Thomsen and Friedenreich (TF) is due to the reaction of naturally occurring TF-antibodies with the carbohydrate group beta-D-Gal-(1-3)-D-GalNAc of desialylated glycophorin A, the major glycoprotein component of the erythrocyte membrane. The specificity of human TF-antibodie...

    journal_title:Vox sanguinis

    pub_type: 杂志文章

    doi:10.1111/j.1423-0410.1985.tb00179.x

    authors: Höppner W,Fischer K,Poschmann A,Paulsen H

    更新日期:1985-01-01 00:00:00

  • Extended storage of platelets in SSP platelet additive solution.

    abstract:BACKGROUND AND OBJECTIVES:The aim of this study was to investigate the effects of extended storage of pooled random platelets in SSP+ additive solution (MacoPharma). MATERIALS AND METHODS:Eight buffy coat-derived, pooled, leucoreduced platelet concentrates were prepared in 75% SSP+, 25% plasma using Fresenius/NPBI Com...

    journal_title:Vox sanguinis

    pub_type: 杂志文章

    doi:10.1111/j.1423-0410.2006.00771.x

    authors: Hornsey VS,McColl K,Drummond O,McMillan L,Morrison A,Morrison L,MacGregor IR,Prowse CV

    更新日期:2006-07-01 00:00:00

  • Standardization of CD62P measurement: results of an international comparative study.

    abstract:BACKGROUND AND OBJECTIVES:Despite long being a mainstay in describing platelet activation via degranulation, interlaboratory variation remains an issue in measurement of membrane CD62P by flow cytometry. Our objective was to identify actions that may minimize this variation. MATERIALS AND METHODS:Sixteen laboratories ...

    journal_title:Vox sanguinis

    pub_type: 临床试验,杂志文章,多中心研究

    doi:10.1111/vox.12023

    authors: Levin E,Serrano K,Devine DV,Biomedical Excellence for Safer Transfusion (BEST) Collaborative.

    更新日期:2013-07-01 00:00:00

  • Monoclonal antibody recognizing a unique Rh-related specificity.

    abstract::A mouse IgG1 monoclonal antibody (MAb) UMRh, was prepared by immunizing Balb/c mice with the Jurkat T cell acute lymphoblastic leukemia (T-ALL) cell line. The MAb UMRh is directed against a widely distributed Rh-related cell surface antigen, present on red blood cells (RBCs) expressing the more common Rh phenotypes. T...

    journal_title:Vox sanguinis

    pub_type: 杂志文章

    doi:10.1111/j.1423-0410.1993.tb03061.x

    authors: Poss MT,Swanson JL,Telen MJ,Lasky LC,Vallera DA

    更新日期:1993-01-01 00:00:00

  • Inactivation of hepatitis A virus by pasteurization and elimination of picornaviruses during manufacture of factor VIII concentrate.

    abstract::Hepatitis A virus (HAV) infections have been reported among hemophiliacs who received factor VIII concentrates which had been purified by ion-exchange chromatography and treated by the solvent detergent (SD) method. Since the virus inactivation procedure of our manufacturing process is heat treatment of the stabilized...

    journal_title:Vox sanguinis

    pub_type: 杂志文章

    doi:

    authors: Hilfenhaus J,Nowak T

    更新日期:1994-01-01 00:00:00

  • A radial immunodiffusion method for the measurement of rat fibrinogen and fibrin degradation products.

    abstract::The present report describes a technique for the production of monospecific rabbit antiserum to rat fibrinogen and its use in a quantitative radial immunodiffusion assay for measurement of rat fibrinogen and fibrin degradation products. It is a suitable technique for measurement of these parameters in small samples wh...

    journal_title:Vox sanguinis

    pub_type: 杂志文章

    doi:10.1111/j.1423-0410.1979.tb04400.x

    authors: Kaplan JE,Blumenstock FA,Saba TM

    更新日期:1979-01-01 00:00:00

  • BS II lectin: a second hemagglutinin isolated from Bandeiraea simplicifolia seeds with affinity for type III polyagglutinable red cells.

    abstract::BS II lectin, a second hemagglutinin isolated from Bandeiraea simplicifolia seeds, agglutinated the red cells from 11 patients manifesting the 'acquired B' antigen phenomenon. The results of serological studies indicate that this lectin has specificity for the type III polyagglutinable receptors rather than for the 'B...

    journal_title:Vox sanguinis

    pub_type: 杂志文章

    doi:10.1111/j.1423-0410.1977.tb04469.x

    authors: Judd WJ,Beck ML,Hicklin BL,Iyer PN,Goldstein IJ

    更新日期:1977-10-01 00:00:00

  • A method for estimating the residual risk of transfusion-transmitted HBV infection associated with occult hepatitis B virus infection in a donor population without universal anti-HBc screening.

    abstract:BACKGROUND AND OBJECTIVES:This report describes a method for estimating the risk of transfusion-transmitted HBV infection attributable to blood components from donors with occult hepatitis B virus infection (OBI) applicable where universal anti-HBc screening is not performed. MATERIALS AND METHODS:In the context of pa...

    journal_title:Vox sanguinis

    pub_type: 杂志文章

    doi:10.1111/vox.12060

    authors: Seed CR,Kiely P

    更新日期:2013-11-01 00:00:00

  • Transmission of hepatitis C virus by anti-HCV-negative blood transfusion. Case report.

    abstract::Acute posttransfusion hepatitis C was reported in a recipient of 3 units of red cells. The recipient became acutely icteric 6 weeks after transfusion, and HCV infection was diagnosed. Stored serum samples of the 3 implicated donations, which were negative with ELISA-2, were retested by PCR and 3rd-generation antibody ...

    journal_title:Vox sanguinis

    pub_type: 杂志文章

    doi:10.1111/j.1423-0410.1995.tb02546.x

    authors: Vrielink H,van der Poel CL,Reesink HW,Zaaijer HL,Lelie PN

    更新日期:1995-01-01 00:00:00

  • Development of white blood cell fragments, during the preparation and storage of platelet concentrates, as measured by using real-time polymerase chain reaction.

    abstract:BACKGROUND AND OBJECTIVES:White blood cell (WBC) fragments may cause human leucocyte antigen (HLA) immunization in recipients. We investigated the occurrence and production of WBC fragments in platelet concentrates (PCs) and plasma units, during storage and filtration, by using real-time polymerase chain reaction (PCR)...

    journal_title:Vox sanguinis

    pub_type: 杂志文章

    doi:10.1111/j.1423-0410.2004.00576.x

    authors: Dijkstra-Tiekstra MJ,van der Schoot CE,Pietersz RN,Huijgens PC,van der Meer PF,Reesink HW

    更新日期:2004-11-01 00:00:00

  • The effect of previous blood transfusion on lymphocyte subsets and natural killer cell function in patients with colorectal cancer.

    abstract::To elucidate the possible influence of previous blood transfusion on immune functions, the transfusion history of 153 patients admitted to hospital for elective colorectal surgery was correlated with lymphocyte subsets and natural killer (NK) cell function. The subsets determined were CD2, CD3, CD4, CD8, CD16, CD20, C...

    journal_title:Vox sanguinis

    pub_type: 杂志文章

    doi:10.1111/j.1423-0410.1994.tb05035.x

    authors: Mathiesen O,Lund L,Brodthagen U,Gandrup P,Grunnet N,Balslev I,Jersild C

    更新日期:1994-01-01 00:00:00

  • Segregation of proteins and sodium in human plasma upon freezing.

    abstract::The proteins and sodium in human source blood plasma are segregated and concentrated on freezing, particularly in plasma that is frozen slowly in a large container. The temperature and the concentration gradients at the freezing front induce a convection in the liquid phase and thus play the primary role in the segreg...

    journal_title:Vox sanguinis

    pub_type: 杂志文章

    doi:10.1111/j.1423-0410.1983.tb01890.x

    authors: Chang CE

    更新日期:1983-01-01 00:00:00

  • Cold lymphocytotoxins. Chromatographical study.

    abstract::A chromatographical study was made of eight antisera containing either anti-HL-A antibodies, cold lymphocytotoxins, or a mixture of both types of antibodies. The results vary according to the sera obtained. Usually cold lymphocytotoxins are sited in the IgM peak and anti-HL-A antibodies in the IgG peak, but there are ...

    journal_title:Vox sanguinis

    pub_type: 杂志文章

    doi:10.1111/j.1423-0410.1976.tb02207.x

    authors: Mayer S,Hauptmann G,Tongio MM

    更新日期:1976-01-01 00:00:00

  • Improvement of enzyme-linked antiglobulin test by using an antiglobulin linked to glucose oxidase: description of the technique.

    abstract::The classic enzyme-linked antiglobulin test (ELAT) used to detect and quantify the amount of IgG antibodies on red blood cells (RBC) is sensitive to hemolysis and erythrocyte enzymatic activities. We describe a new ELAT by using glucose oxidase (GO) linked to antihuman IgG. The optical density base line of GO-ELAT, al...

    journal_title:Vox sanguinis

    pub_type: 杂志文章

    doi:10.1111/j.1423-0410.1984.tb00098.x

    authors: Rigal D,Monestier M,Lafont S,Raffin T,Got A,Meyer F,Jouvenceaux A

    更新日期:1984-01-01 00:00:00

  • Assessment of methods for cytomegalovirus antibody testing of blood donations.

    abstract::As part of a programme to establish a panel of individuals with high titres of anticytomegalovirus (CMV) IgG for the production of CMV Ig, the CMV serostatus of 139 normal blood donors was determined by indirect haemagglutination assay, enzyme immunoassay, and complement fixation. The indirect haemagglutination assay ...

    journal_title:Vox sanguinis

    pub_type: 杂志文章

    doi:10.1111/j.1423-0410.1984.tb04137.x

    authors: Chung A,Chang W,Leung MK,Ofosu FA,Moore BP

    更新日期:1984-01-01 00:00:00

  • Effect of rate and delay of cooling during initial cooling process: in vitro effect on red cells.

    abstract:BACKGROUND:Whole blood is stored at room temperature (RT) until processing into components. After separation and filtration, the RCC has to be cooled from RT to +2 - 6°C. Different start times of the cooling process and different cooling rates can be encountered in daily routine. The effect of these parameters of the i...

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    pub_type: 杂志文章

    doi:10.1111/j.1423-0410.2010.01458.x

    authors: Bontekoe IJ,van der Meer PF,de Korte D

    更新日期:2011-07-01 00:00:00

  • Persistence of Parvovirus B19 (B19V) DNA and humoral immune response in B19V-infected blood donors.

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    journal_title:Vox sanguinis

    pub_type: 杂志文章

    doi:10.1111/vox.12162

    authors: Juhl D,Görg S,Hennig H

    更新日期:2014-10-01 00:00:00

  • Results of a multicentre clinical trial of the solid-phase enzyme immunoassay for hepatitis B surface antigen.

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    journal_title:Vox sanguinis

    pub_type: 杂志文章

    doi:10.1111/j.1423-0410.1978.tb02902.x

    authors: Kacaki J,Wolters G,Kuijpers L,Stulemeyer S

    更新日期:1978-07-01 00:00:00

  • Reticulocyte count in red-blood-cell units stored in AS-1.

    abstract:BACKGROUND AND OBJECTIVES:Previous data that showed maintenance of reticulocyte percentage in whole blood stored in CPDA-1 have led to the assumption that reticulocyte maturation becomes arrested during refrigerated storage. However, reticulocyte behaviour in red-blood-cell units stored in additive solutions has not ye...

    journal_title:Vox sanguinis

    pub_type: 杂志文章

    doi:10.1111/vox.12011

    authors: Urbina A,Palomino F

    更新日期:2013-05-01 00:00:00

  • Errors in interpreting the pretransfusion bedside compatibility test: an experimental study.

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    journal_title:Vox sanguinis

    pub_type: 杂志文章

    doi:10.1159/000031147

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    更新日期:2000-01-01 00:00:00