Development of white blood cell fragments, during the preparation and storage of platelet concentrates, as measured by using real-time polymerase chain reaction.

Abstract:

BACKGROUND AND OBJECTIVES:White blood cell (WBC) fragments may cause human leucocyte antigen (HLA) immunization in recipients. We investigated the occurrence and production of WBC fragments in platelet concentrates (PCs) and plasma units, during storage and filtration, by using real-time polymerase chain reaction (PCR) and flow cytometry. MATERIALS AND METHODS:To study the occurrence of WBC fragments, 'male' WBCs were spiked into double-filtered 'female' PCs in a concentration series of 0.03-100 WBCs/microl (n = 4 per level). To study the production of WBC fragments, 'male' WBCs were spiked into 'female' plasma units to 4 x 10(9) WBCs/l and stored at room temperature prior to filtration (n = 4 per storage time; t = 0, 24 or 48 h). DNA was measured by both albumin real-time PCR and Y real-time PCR. Intact WBCs were counted by using flow cytometry. The number of WBC fragments was calculated by subtracting cell-free DNA (real-time PCR on supernatant) and intact WBCs (flow cytometry) from the total DNA amount (real-time PCR). RESULTS:Spiking of 'male' WBCs into 'female' PCs showed that the Y real-time PCR is linear and has a reproducible quantitative range down to 0.03 WBC/microl, but that the albumin-PCR, in unspiked samples, revealed a total of 6-10 WBC equivalents/microl (eq/microl). After centrifugation, half of this was observed as cell-free DNA in the supernatant, suggesting that the remaining DNA is derived from WBC fragments. The number of intact WBCs, amount of cell-free DNA and number of WBC fragments after filtration increased significantly when filtration was delayed for up to 48 h, from 0.1 WBC/microl, 1.3 WBC eq/microl and 0.6 WBC eq/microl at t = 0 h to 25 WBC/microl, 38 WBC eq/microl and 57 WBC eq/microl at t = 48 h, respectively. CONCLUSIONS:WBC fragments occur in WBC-reduced PCs and increase when products are stored, prior to filtration, up to levels that are equivalent to the amounts of intact WBCs that induce HLA immunization (i.e. > 5 x 10(6)/unit).

journal_name

Vox Sang

journal_title

Vox sanguinis

authors

Dijkstra-Tiekstra MJ,van der Schoot CE,Pietersz RN,Huijgens PC,van der Meer PF,Reesink HW

doi

10.1111/j.1423-0410.2004.00576.x

subject

Has Abstract

pub_date

2004-11-01 00:00:00

pages

250-6

issue

4

eissn

0042-9007

issn

1423-0410

pii

VOX576

journal_volume

87

pub_type

杂志文章
  • Riboflavin and amotosalen photochemical treatments of platelet concentrates reduce thrombus formation kinetics in vitro.

    abstract:BACKGROUND:Photochemical treatment (PCT) of platelet concentrates using photosensitizers and ultraviolet light illumination reduces the proliferation potential of pathogens by damaging biomolecules. MATERIALS AND METHODS:The impact of riboflavin (RF-PRT)- and amotosalen (AS-PCT)-based pathogen inactivation on platelet...

    journal_title:Vox sanguinis

    pub_type: 杂志文章

    doi:10.1111/vox.12231

    authors: Van Aelst B,Feys HB,Devloo R,Vanhoorelbeke K,Vandekerckhove P,Compernolle V

    更新日期:2015-05-01 00:00:00

  • Haemolytic disease of the fetus and newborn.

    abstract::Haemolytic Disease of the Fetus and Newborn (HDFN) is caused by maternal alloimmunization against red blood cell antigens. In severe cases, HDFN may lead to fetal anaemia with a risk for fetal death and to severe forms of neonatal hyperbilirubinaemia with a risk for kernicterus. Most severe cases are caused by anti-D,...

    journal_title:Vox sanguinis

    pub_type: 杂志文章,评审

    doi:10.1111/vox.12265

    authors: de Haas M,Thurik FF,Koelewijn JM,van der Schoot CE

    更新日期:2015-08-01 00:00:00

  • Anti-granulocyte antibody screening with extracted granulocyte antigens by a micro-mixed passive hemagglutination method.

    abstract:BACKGROUND AND OBJECTIVES:Serologic tests for granulocyte antibodies, i.e., the granulocyte agglutination test and the granulocyte immunofluorescence test, require panels of typed granulocytes that cannot be preserved for more than a few hours. We have developed a new method in which granulocyte antigens, extracted int...

    journal_title:Vox sanguinis

    pub_type: 临床试验,杂志文章

    doi:10.1159/000031073

    authors: Araki N,Nose Y,Kohsaki M,Mito H,Ito K

    更新日期:1999-01-01 00:00:00

  • Collection and use of stem cells; role of transfusion centers in bone marrow transplantation.

    abstract::Marrow transplantation is increasing, hematopoietic stem cells are being obtained from a wider variety of donors, and the cells almost always undergo some processing steps. These activities, along with the hematologic complexities of marrow transplant patients, create many varied opportunities for blood centers. ...

    journal_title:Vox sanguinis

    pub_type: 杂志文章,评审

    doi:10.1111/j.1423-0410.1994.tb04541.x

    authors: McCullough J

    更新日期:1994-01-01 00:00:00

  • Simultaneous occurrence of anti-F1 and anti-I cold agglutinins in a patient's serum.

    abstract::The second example of anti-F1 cold agglutinins recognizing a developmentally regulated antigen like I is described. The antibody occurred transiently and simultaneously together with an anti-I cold agglutinin. Although anti-F1 and anti-I specificities are entirely different, the data on the biochemistry of F1 and I an...

    journal_title:Vox sanguinis

    pub_type: 杂志文章

    doi:10.1111/j.1423-0410.1984.tb01573.x

    authors: Roelcke D,Weber MT

    更新日期:1984-01-01 00:00:00

  • Solvent/detergent treatment of human plasma--a very robust method for virus inactivation. Validated virus safety of OCTAPLAS.

    abstract::OCTAPLAS is a cell-free standardized blood group specific human coagulation active plasma for transfusion. The viral safety is mainly based on the treatment with solvent/detergent. SD is known to irreversibly inactivate the lipid enveloped viruses including HIV 1 + 2, HBV and HCV and has been recommended both by Europ...

    journal_title:Vox sanguinis

    pub_type: 杂志文章,评审

    doi:10.1111/j.1423-0410.1998.tb05474.x

    authors: Biesert L,Suhartono H

    更新日期:1998-01-01 00:00:00

  • Selective depression of blood group antigens associated with hereditary ovalocytosis among melanesians.

    abstract::Recessively inherited ovalocytosis in coastal Melanesians is associated with widespread, but selective, depression of blood group antigens in homozygotes, who comprise about 15% of these populations. It is suggested that a membrane anomaly exists, and that the series of depressed determinants all depend, for their ful...

    journal_title:Vox sanguinis

    pub_type: 杂志文章

    doi:10.1111/j.1423-0410.1977.tb00612.x

    authors: Booth PB,Serjeantson S,Woodfield DG,Amato D

    更新日期:1977-01-01 00:00:00

  • Preparation of human immunoglobulin free of plasmin and anticomplement activities.

    abstract::Human IgG separated by Cohn fractionation showed variability in the content of aggregates, plasminogen and anticomplement activity. The plasminogen was removed or markedly reduced by affinity chromatography on Sepharose-lysine. Anticomplement activity was reduced by chromatography of Cohn fraction II on DEAE-cellulose...

    journal_title:Vox sanguinis

    pub_type: 杂志文章

    doi:10.1111/j.1423-0410.1977.tb00619.x

    authors: Habeeb AF,Francis RD

    更新日期:1977-01-01 00:00:00

  • Standardization of CD62P measurement: results of an international comparative study.

    abstract:BACKGROUND AND OBJECTIVES:Despite long being a mainstay in describing platelet activation via degranulation, interlaboratory variation remains an issue in measurement of membrane CD62P by flow cytometry. Our objective was to identify actions that may minimize this variation. MATERIALS AND METHODS:Sixteen laboratories ...

    journal_title:Vox sanguinis

    pub_type: 临床试验,杂志文章,多中心研究

    doi:10.1111/vox.12023

    authors: Levin E,Serrano K,Devine DV,Biomedical Excellence for Safer Transfusion (BEST) Collaborative.

    更新日期:2013-07-01 00:00:00

  • De novo protein synthesis in mature platelets: a consideration for transfusion medicine.

    abstract::Platelet function in thrombosis and haemostasis is reasonably well understood at the molecular level with respect to the proteins involved in cellular structure, signalling networks and platelet interaction with clotting factors and other cells. However, the natural history of these proteins has only recently garnered...

    journal_title:Vox sanguinis

    pub_type: 杂志文章,评审

    doi:10.1111/j.1423-0410.2010.01333.x

    authors: Schubert P,Devine DV

    更新日期:2010-08-01 00:00:00

  • Cord blood banking and transplant in Europe. Eurocord.

    abstract::The number of cord blood transplants has been increasing very quickly with more than 250 cases reported to Eurocord Registry and more than 500 patients transplanted via the New York Cord Blood Bank. Cord blood transplants have been performed either with related or unrelated cord blood. Several cord blood banks establi...

    journal_title:Vox sanguinis

    pub_type: 杂志文章,评审

    doi:10.1111/j.1423-0410.1998.tb05403.x

    authors: Gluckman E,Rocha V,Chastang C

    更新日期:1998-01-01 00:00:00

  • Transfusion risk associated with recent arbovirus outbreaks in French Polynesia.

    abstract:BACKGROUND AND OBJECTIVES:French Polynesia, where dengue virus (DENV) has been present for a long time, experienced two successive outbreaks of Zika (ZIKV) and chikungunya viruses (CHIKV) between 2013 and 2015. To avoid the transmission of these viruses by transfusion, nucleic acid testing (NAT) has been in place for D...

    journal_title:Vox sanguinis

    pub_type: 杂志文章

    doi:10.1111/vox.12855

    authors: Beau F,Mallet HP,Lastère S,Broult J,Laperche S

    更新日期:2020-02-01 00:00:00

  • Novel polymorphisms in exons 6 and 7 of A/B alleles detected by polymerase chain reaction-single strand conformation polymorphism.

    abstract:BACKGROUND AND OBJECTIVES:The ABO blood group system is the most important blood group system in transfusion medicine. In addition to the major A, B and O alleles, many rare alleles have been defined. Polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) and analysis by PCR sequence specific pri...

    journal_title:Vox sanguinis

    pub_type: 杂志文章

    doi:10.1111/j.1423-0410.2005.00729.x

    authors: Chen YJ,Chen PS,Liu HM,Lyou JY,Hu HY,Lin JS,Tzeng CH

    更新日期:2006-02-01 00:00:00

  • The D antigen characteristic of RoHar is a partial D antigen.

    abstract::The results of testing RoHarr cells with panels of monoclonal anti-D suggest that the D antigen (referred to as DHar) encoded by the haplotype (D)c(e) Rh33 is a partial D antigen. IgM monoclonal anti-D are more efficient than IgG monoclonal anti-D in detecting DHar.DHar expresses some but not all of both epD5 and epD6...

    journal_title:Vox sanguinis

    pub_type: 杂志文章

    doi:10.1111/j.1423-0410.1996.tb01317.x

    authors: Wallace M,Lomas-Francis C,Tippett P

    更新日期:1996-01-01 00:00:00

  • Biochemistry of whole blood in poly(ethylene-co-ethylacrylate) experimental blood containers.

    abstract::The biochemical status of whole blood stored in containers fabricated of ethylene ethylacrylate (EEA) film was monitored at several times during 4 weeks of storage at 4 degrees C. Fifteen biochemical indicators were studied to reflect on erythrocyte integrity, cellular metabolism, plasma protein stability, and microag...

    journal_title:Vox sanguinis

    pub_type: 杂志文章

    doi:10.1111/j.1423-0410.1985.tb00161.x

    authors: Horowitz B,Rowe AW,Stryker MH,Van der Sande J,Waldman AA,Wong SC,Gass JD,Woods KR

    更新日期:1985-01-01 00:00:00

  • Blood transfusion in sickle cell disease.

    abstract::Blood transfusion is used as a life-saving and prophylactic treatment in sickle cell disease. Despite the many complications associated with its use, few randomised controlled trials and careful research studies have been performed to fully define its role. This subjects is, therefore, discussed in the context of the ...

    journal_title:Vox sanguinis

    pub_type: 杂志文章,评审

    doi:10.1111/j.1423-0410.1995.tb03916.x

    authors: Davies SC,Olatunji PO

    更新日期:1995-01-01 00:00:00

  • A comparative study of platelets stored in polyvinyl chloride containers plasticised with butyryl trihexyl citrate or triethylhexyl trimellitate.

    abstract::Platelet concentrates (PCs), stored for 5 days in PL 2209, a new polyvinyl chloride (PVC) storage container plasticised with butyryl trihexyl citrate, were compared with those stored in PL 1240, a PVC platelet container plasticised with triethylhexyl trimellitate. In part 1 of the study, pooled platelet-rich plasma (P...

    journal_title:Vox sanguinis

    pub_type: 杂志文章

    doi:10.1111/j.1423-0410.1995.tb02593.x

    authors: Turner VS,Mitchell SG,Kang SK,Hawker RJ

    更新日期:1995-01-01 00:00:00

  • Pulmonary complications during granulocyte transfusions: incidence and clinical features.

    abstract::The use of granulocyte transfusions in profoundly neutropenic patients has increased markedly in recent years. Whenever a pulmonary infiltrate develops during the course of these transfusions, the question arises as to what role the transfusions are playing and whether the transfusions should be discontinued to preven...

    journal_title:Vox sanguinis

    pub_type: 杂志文章

    doi:

    authors: Karp DD,Ervin TJ,Tuttle S,Gorgone BC,Lavin P,Yunis EJ

    更新日期:1982-02-01 00:00:00

  • Use of a (proposed) standard protocol to validate Terumo TSCD-II connections between dissimilar blood bag tubing.

    abstract:BACKGROUND AND OBJECTIVES:ISO standards for blood bags do not adequately define and control the dimensions of blood bag transfer tubing. This lack of standardization presents potential difficulties when making sterile connections between the wide range of tubing that has evolved in the absence of such standards. We aim...

    journal_title:Vox sanguinis

    pub_type: 杂志文章

    doi:10.1111/j.1423-0410.2006.00832.x

    authors: Nightingale MJ,Lees B,Biset R,Mertens W

    更新日期:2006-10-01 00:00:00

  • The effect of immunoadsorption with the Immusorba TR-350 column on coagulation compared to plasma exchange.

    abstract:BACKGROUND AND OBJECTIVES:Plasma exchange (PE) and immunoadsorption with the Immusorba TR-350 column (IA) are used to remove autoantibodies from plasma in acute neurological autoimmune disorders. The impact of IA on coagulation and on low molecular weight heparin (LMWH) levels in comparison with PE was investigated. P...

    journal_title:Vox sanguinis

    pub_type: 杂志文章

    doi:10.1111/vox.12191

    authors: Koessler J,Kobsar A,Kuhn S,Koessler A,Yilmaz P,Weinig E,Putz E,Boeck M,Klinker E

    更新日期:2015-01-01 00:00:00

  • Accumulation of soluble inflammatory mediators between blood donation and pre-storage leucocyte depletion.

    abstract:BACKGROUND AND OBJECTIVES:Leucocyte-derived cytokines accumulate in stored whole blood. Pre-storage leucocyte depletion has reduced cytokine levels and, consequently, febrile non-haemolytic transfusion reactions. As leucocyte filtration and component separation can be performed until 24 h after donation, we hypothesize...

    journal_title:Vox sanguinis

    pub_type: 杂志文章

    doi:10.1111/j.1423-0410.2008.01132.x

    authors: Chudziak D,Sireis W,Pfeiffer HU,Henschler R,Seifried E,Bönig H

    更新日期:2009-02-01 00:00:00

  • Unusual serological response to hepatitis E virus in plasma donors consistent with re-infection.

    abstract::Hepatitis E virus (HEV)-positive plasma donations, identified by a plasma mini-pool screening approach, were analysed using serological methods for the presence of anti-HEV IgM and IgG. Avidity testing was performed on the IgG-reactive donations. Anti-HEV IgG with high avidity was observed in two donors together with ...

    journal_title:Vox sanguinis

    pub_type: 杂志文章

    doi:10.1111/vox.12294

    authors: Baylis SA,Crossan C,Corman VM,Blümel J,Scobie L,Dalton HR

    更新日期:2015-11-01 00:00:00

  • Occurrence and characterization of lympho-agglutinins in Indian plants.

    abstract::A survey of the occurrence of lympho-agglutinins was carried out on seeds of 150 species of wild and cultivated plants. Potent agglutinins were detected in 17 extracts by employing lymphocytes of man, guinea pig, goat and sheep in the bioassay. Except for one species each of Mimosoideae, Euphorbiaceae and Clusiaceae, ...

    journal_title:Vox sanguinis

    pub_type: 杂志文章

    doi:10.1111/j.1423-0410.1982.tb00034.x

    authors: Sandhu RS,Kamboj SS,Reen RS,Arora JS

    更新日期:1982-01-01 00:00:00

  • Rare and transient anti-D antibody response in D(-) liver transplant recipients transfused with D(+) red blood cells.

    abstract::A retrospective analysis was conducted on 20 D(-) liver transplant (LT) recipients transfused with D(+) RBCs perioperatively and screened for RBC antibodies between 2 and 6 months later. None developed anti-D detectable by the indirect antiglobulin test. Two patients produced weak anti-D that reacted only with papain-...

    journal_title:Vox sanguinis

    pub_type: 杂志文章

    doi:10.1111/vox.12392

    authors: Burin des Roziers N,Ibanez C,Samuel D,Francoz C,Idri S,François A,Mortelecque R,Bierling P,Pirenne F

    更新日期:2016-07-01 00:00:00

  • Single-dose intravenous iron infusion versus red blood cell transfusion for the treatment of severe postpartum anaemia: a randomized controlled pilot study.

    abstract:BACKGROUND AND OBJECTIVES:There are no randomized trials comparing intravenous iron to RBC transfusion for the treatment of severe postpartum anaemia. The objectives of this study were to evaluate the feasibility of randomizing women with severe postpartum anaemia secondary to postpartum haemorrhage to RBC transfusion ...

    journal_title:Vox sanguinis

    pub_type: 临床试验,杂志文章,随机对照试验

    doi:10.1111/vox.12475

    authors: Holm C,Thomsen LL,Norgaard A,Langhoff-Roos J

    更新日期:2017-02-01 00:00:00

  • Study of coagulation function in thawed apheresis plasma for photochemical treatment by amotosalen and UVA.

    abstract:BACKGROUND AND OBJECTIVES:Photochemical treatment (PCT) based on amotosalen and ultraviolet A light (UVA) demonstrated a wide range of pathogen inactivation. However, coagulation proteins are affected by this treatment. The aim of this study was to evaluate the coagulation parameters in apheresis plasma units after tha...

    journal_title:Vox sanguinis

    pub_type: 杂志文章

    doi:10.1111/j.1423-0410.2008.001147.x

    authors: de Valensart N,Rapaille A,Goossenaerts E,Sondag-Thull D,Deneys V

    更新日期:2009-04-01 00:00:00

  • The impact of vaccination on RBC alloimmunization in a murine model.

    abstract::Emerging data in animal models and humans suggest that pathogen-associated and damage-associated molecular patterns variably impact RBC alloantibody formation. In this study, we tested the hypothesis that vaccinations may enhance immune responses to transfused RBCs. The Pneumovax23 vaccine decreased the magnitude of a...

    journal_title:Vox sanguinis

    pub_type: 杂志文章

    doi:10.1111/vox.12541

    authors: Natarajan P,Santhanakrishnan M,Tormey CA,Hendrickson JE

    更新日期:2017-08-01 00:00:00

  • Splenic lymphoma presenting as warm autoimmune hemolytic anemia associated with pure red cell aplasia.

    abstract:BACKGROUND AND OBJECTIVE:Warm autoimmune hemolytic anemia (AIHA) is a condition in which peripheral red blood cell (RBC) destruction is induced by the presence of an autoantibody. Pure red cell aplasia (PRCA) represents an isolated process of decreased erythropoiesis. The combination of both is quite rare, with a very ...

    journal_title:Vox sanguinis

    pub_type: 杂志文章

    doi:10.1159/000031163

    authors: Zeidman A,Fradin Z,Barac Y,Bendayan D,Mittelman M,Orlin J

    更新日期:2000-01-01 00:00:00

  • External quality assessment of human neutrophil antigen (HNA)-specific antibody detection and HNA genotyping from 2000 to 2012.

    abstract::Since 2000, Quality Assurance (QA) exercises for the detection and identification of granulocyte antibodies and DNA typing for human neutrophil antigens (HNA) have been distributed within the International Granulocyte Immunobiology Workshops, which are linked to International Society of Blood Transfusion. The exercise...

    journal_title:Vox sanguinis

    pub_type: 杂志文章

    doi:10.1111/vox.12041

    authors: Lucas G,Porcelijn L,Fung YL,Green F,Reil A,Hopkins M,Schuller R,Green A,de Haas M,Bux J

    更新日期:2013-10-01 00:00:00

  • Haemolytic anaemia to the alpha-interferon treatment: a proposed mechanism.

    abstract::Auto-immune haemolytic anaemia (AIHA) has been found in a case of alpha-interferon treatment. Serum antibody and eluate were positive in the absence of the drug. Although the patient recovered after the treatment was stopped, DAGT remained positive for at least 8 months. The mechanism proposed to explain why this drug...

    journal_title:Vox sanguinis

    pub_type: 杂志文章

    doi:10.1111/j.1423-0410.1993.tb02135.x

    authors: Barbolla L,Paniagua C,Outeiriño J,Prieto E,Sánchez Fayos J

    更新日期:1993-01-01 00:00:00