Improvement of enzyme-linked antiglobulin test by using an antiglobulin linked to glucose oxidase: description of the technique.

Abstract:

:The classic enzyme-linked antiglobulin test (ELAT) used to detect and quantify the amount of IgG antibodies on red blood cells (RBC) is sensitive to hemolysis and erythrocyte enzymatic activities. We describe a new ELAT by using glucose oxidase (GO) linked to antihuman IgG. The optical density base line of GO-ELAT, alkaline phosphatase-ELAT and peroxidase-ELAT were, respectively, 0.180, 0.350 and 0.550. This very low baseline of GO-ELAT was due to the absence of hemolysis (the pH of the GO substrate is 6.5). This technique is ten times more sensitive than the indirect antiglobulin test and detects up to 1 ng/ml of anti-D alloantibodies. Additional advantages of the technique are (1) there is no intrinsic GO enzyme in RBC, and (2) it is not necessary to fix the RBC.

journal_name

Vox Sang

journal_title

Vox sanguinis

authors

Rigal D,Monestier M,Lafont S,Raffin T,Got A,Meyer F,Jouvenceaux A

doi

10.1111/j.1423-0410.1984.tb00098.x

subject

Has Abstract

pub_date

1984-01-01 00:00:00

pages

349-54

issue

6

eissn

0042-9007

issn

1423-0410

journal_volume

46

pub_type

杂志文章
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