Abstract:
:The classic enzyme-linked antiglobulin test (ELAT) used to detect and quantify the amount of IgG antibodies on red blood cells (RBC) is sensitive to hemolysis and erythrocyte enzymatic activities. We describe a new ELAT by using glucose oxidase (GO) linked to antihuman IgG. The optical density base line of GO-ELAT, alkaline phosphatase-ELAT and peroxidase-ELAT were, respectively, 0.180, 0.350 and 0.550. This very low baseline of GO-ELAT was due to the absence of hemolysis (the pH of the GO substrate is 6.5). This technique is ten times more sensitive than the indirect antiglobulin test and detects up to 1 ng/ml of anti-D alloantibodies. Additional advantages of the technique are (1) there is no intrinsic GO enzyme in RBC, and (2) it is not necessary to fix the RBC.
journal_name
Vox Sangjournal_title
Vox sanguinisauthors
Rigal D,Monestier M,Lafont S,Raffin T,Got A,Meyer F,Jouvenceaux Adoi
10.1111/j.1423-0410.1984.tb00098.xsubject
Has Abstractpub_date
1984-01-01 00:00:00pages
349-54issue
6eissn
0042-9007issn
1423-0410journal_volume
46pub_type
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