Molecular basis of Tn-polyagglutinability.

Abstract:

:Spectrophotometric and gas-liquid chromatographic analyses on the carbohydrate moiety of tryptic erythrocyte glycopeptides from persons with Tn-syndrome reveal a selective lowering of the galactose and sialic acid content, the degree being dependent on the percentage of polyagglutinable cells. Alkaline borohydride specifically releases N-acetylgalactosaminitol, and the amount is correlated to the percentage of pathological acetylgalactosaminitol, and the amount is correlated to the percentage of pathological erythrocytes. It is concluded that the alkali-labile carbohydrate chains of Tn-polyagglutinable red cells solely consist of N-acetylgalactosamine linked to serine or threonine. Experiments with heterophile agglutinins whose specificity is known are in line with the above-mentioned results. As judged from SDS-polyacrylamide gel electrophoresis the three major membrane glycoproteins are affected to a different extent by the defect.

journal_name

Vox Sang

journal_title

Vox sanguinis

authors

Dahr W,Uhlenbruck G,Gunson HH,Van Der Hart M

doi

10.1111/j.1423-0410.1975.tb00475.x

subject

Has Abstract

pub_date

1975-01-01 00:00:00

pages

36-50

issue

1

eissn

0042-9007

issn

1423-0410

journal_volume

29

pub_type

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