Collaborative study to calibrate hepatitis C virus genotypes 2-6 against the HCV International Standard, 96/790 (genotype 1).

Abstract:

BACKGROUND AND OBJECTIVES:A major requirement of a hepatitis C virus (HCV) RNA nucleic acid amplification technology (NAT) assay validation is the ability of the assay to detect the six major genotypes of HCV with equivalent sensitivities. The aim of this study was to characterize and calibrate an HCV genotype panel for use in such studies. MATERIALS AND METHODS:Panels consisting of the first International Standard (IS) for HCV RNA NAT assays (96/790; HCV genotype 1a) and isolates of genotypes 2-6 were sent to 17 laboratories worldwide which use a variety of NAT tests, both qualitative and quantitative. The HCV RNA content of each panel member was determined and the mean titre calculated in International Units/ml (IU/ml). RESULTS:The calculated mean titres (calibrated against the HCV International Standard), in log10 IU/ml, of the genotype 2-6 samples were 3.99, 3.81, 4.14, 4.18 and 4.61, respectively. CONCLUSIONS:An HCV genotype panel, calibrated in IU/ml, has been established and should be valuable for assay validation. All the genotypes were detected by all the assays used, but it was not possible to demonstrate that the genotypes were detected with equal efficiencies.

journal_name

Vox Sang

journal_title

Vox sanguinis

authors

Saldanha J,Heath A,Collaborative Study Group.

doi

10.1046/j.1423-0410.2003.00260.x

subject

Has Abstract

pub_date

2003-01-01 00:00:00

pages

20-7

issue

1

eissn

0042-9007

issn

1423-0410

pii

260

journal_volume

84

pub_type

杂志文章
  • Report of the Second International Workshop on molecular blood group genotyping.

    abstract::The second International Society of Blood Transfusion and International Council for Standardization in Haematology workshop on molecular blood group genotyping was held in 2006. Forty-one laboratories participated. Six samples were distributed: two representing DNA from transfusion-dependent patients for testing for a...

    journal_title:Vox sanguinis

    pub_type:

    doi:10.1111/j.1423-0410.2007.00926.x

    authors: Daniels G,van der Schoot CE,Olsson ML

    更新日期:2007-07-01 00:00:00

  • Phenotype prediction by DNA-based typing of clinically significant blood group systems in Jordanian blood donors.

    abstract:BACKGROUND AND OBJECTIVES:During the past 10 years several DNA-typing methods have been developed to complement routine serological typing for determination of polymorphisms in the ABO, RH, KEL, JK and FY blood group genes. However, the molecular basis of blood groups can differ widely between ethnic groups. The purpos...

    journal_title:Vox sanguinis

    pub_type: 杂志文章

    doi:10.1046/j.1423-0410.2002.00182.x

    authors: Irshaid NM,Ramadan S,Wester ES,Olausson P,Hellberg A,Merza JY,Olsson ML

    更新日期:2002-07-01 00:00:00

  • Supernatants of stored polymorphonuclear neutrophils exhibit growth-promoting activity in several cell lines: perforin expression in polymorphonuclear neutrophils and its role in down-regulation of growth-promoting activity.

    abstract:BACKGROUND AND OBJECTIVES:Polymorphonuclear neutrophils (PMNs) play important roles in the host immune defence. This study was performed to identify roles of PMNs other than those already known. MATERIALS AND METHODS:PMNs were separated from the peripheral blood of healthy individuals and stored in vitro for 24 h in t...

    journal_title:Vox sanguinis

    pub_type: 杂志文章

    doi:10.1046/j.1423-0410.2002.00156.x

    authors: Nishimura M,Mitsunaga S,Juji T

    更新日期:2002-04-01 00:00:00

  • Placental transfer of Rh antibody (anti-D IgG) during pregnancy.

    abstract::Anti-D levels in blood taken immediately postpartum from Rh-negative women who have been given antenatal Rh immune globulin and the anti-D levels in the cord blood of their babies were measured semiquantitatively by a low ionic polybrene Auto Analyzer technique. When both mother and baby were Rh-negative a comparison ...

    journal_title:Vox sanguinis

    pub_type: 杂志文章

    doi:10.1111/j.1423-0410.1982.tb00031.x

    authors: Pollock JM,Bowman JM

    更新日期:1982-01-01 00:00:00

  • Persistent aggregates in apheresis platelet concentrates.

    abstract:BACKGROUND:Aggregates often appear during apheresis. Sometimes, these persist throughout storage, causing product wastage. This study assessed product quality of apheresis concentrates containing persistent aggregates (PA) and aimed to identify the factors that contribute to their formation. METHODS:Donation (n = 180)...

    journal_title:Vox sanguinis

    pub_type: 杂志文章

    doi:10.1111/vox.12243

    authors: Feys HB,Coene J,Devloo R,Van Aelst B,Pottel H,Vandekerckhove P,Compernolle V

    更新日期:2015-05-01 00:00:00

  • Severe neonatal alloimmune thrombocytopenia due to anti-HPA-3a.

    abstract:BACKGROUND AND OBJECTIVES:Neonatal alloimmune thrombocytopenia is usually attributable to HPA-la antibodies. We report a case of parental platelet antigen incompatibility associated with a severe neonatal thrombocytopenia secondary to alloimmunization to HPA-3a. MATERIALS AND METHODS:Platelet antibodies were detected ...

    journal_title:Vox sanguinis

    pub_type: 杂志文章

    doi:

    authors: Boehlen F,Kaplan C,de Moerloose P

    更新日期:1998-01-01 00:00:00

  • The role of HIV infectivity and composition of factor VIII concentrates on the immunity of haemophiliacs positive for HIV antibodies.

    abstract::Forty-six subjects (44 HIV antibody-positive) with some degree of immune deficiency (at least TH/TS ratio below 1) were randomly distributed into 4 treatment groups. Each group was assigned to 1 of 4 products to be used exclusively for a 1-year period: 1 concentrate was of intermediate purity and not heat-treated, and...

    journal_title:Vox sanguinis

    pub_type: 临床试验,杂志文章,随机对照试验

    doi:10.1111/j.1423-0410.1987.tb04911.x

    authors: Allain JP,Frommel D,Bosser C,Gazengel C,Larrieu MJ,Sultan Y

    更新日期:1987-01-01 00:00:00

  • Detection of a new weak A blood-group allele (Aw11).

    abstract:BACKGROUND AND OBJECTIVES:Weak ABO variants may escape tests using unlicensed sera. MATERIALS AND METHODS:Prior to transfusion, ABO grouping was performed using an automated system and in-house diluted sera, and manual and bedside test techniques. Genotyping and sequencing were performed using standard methods. RESUL...

    journal_title:Vox sanguinis

    pub_type: 杂志文章

    doi:10.1111/j.1423-0410.2006.00752.x

    authors: Pruss A,Heymann GA,Braun J,Kiesewetter HH,Salama A

    更新日期:2006-04-01 00:00:00

  • Detection of platelet antibody using rosette technique with anti-IgG antibody-coated polyacrylamide gel: a preliminary report.

    abstract::Paraformaldehyde-fixed platelets from normal donors were used for detection of antibody to platelet by in vitro sensitization (indirect method) utilizing rabbit anti-human IgG heavy chain specific antibody-coated polyacrylamide gels (Immunobeads). The sensitized platelets formed rosettes with Immunobeads and the posit...

    journal_title:Vox sanguinis

    pub_type: 杂志文章

    doi:10.1111/j.1423-0410.1980.tb01869.x

    authors: Salmassi S,Yokoyama MM,Maples JA,Matsui Y

    更新日期:1980-11-01 00:00:00

  • Segregation of proteins and sodium in human plasma upon freezing.

    abstract::The proteins and sodium in human source blood plasma are segregated and concentrated on freezing, particularly in plasma that is frozen slowly in a large container. The temperature and the concentration gradients at the freezing front induce a convection in the liquid phase and thus play the primary role in the segreg...

    journal_title:Vox sanguinis

    pub_type: 杂志文章

    doi:10.1111/j.1423-0410.1983.tb01890.x

    authors: Chang CE

    更新日期:1983-01-01 00:00:00

  • A method for estimating the residual risk of transfusion-transmitted HBV infection associated with occult hepatitis B virus infection in a donor population without universal anti-HBc screening.

    abstract:BACKGROUND AND OBJECTIVES:This report describes a method for estimating the risk of transfusion-transmitted HBV infection attributable to blood components from donors with occult hepatitis B virus infection (OBI) applicable where universal anti-HBc screening is not performed. MATERIALS AND METHODS:In the context of pa...

    journal_title:Vox sanguinis

    pub_type: 杂志文章

    doi:10.1111/vox.12060

    authors: Seed CR,Kiely P

    更新日期:2013-11-01 00:00:00

  • Benefits and complications of regular blood transfusion in patients with beta-thalassaemia major.

    abstract::Early and regular blood transfusion therapy in patients with homozygous beta-thalassaemia decreases the complications of severe anaemia and prolongs survival. In the long term, however, the beneficial effects of transfusions are limited by the organ damage resulting from iron overload, a consequence of the body's limi...

    journal_title:Vox sanguinis

    pub_type: 杂志文章,评审

    doi:10.1159/000031230

    authors: Prati D

    更新日期:2000-01-01 00:00:00

  • Monocyte-erythrocyte interaction in autoimmune haemolytic anaemia in relation to the number of erythrocyte-bound IgG molecules and subclass specificity of autoantibodies.

    abstract::Monocyte-erythrocyte interaction in patients with autoimmune haemolytic anaemia (AIHA) was assessed by phagocytosis and rosette assays. In most patients, a relationship was observed between haemolysis and the phagocytosis of their own erythrocytes by allogenic peripheral monocytes. An evaluation of the number of immun...

    journal_title:Vox sanguinis

    pub_type: 杂志文章

    doi:10.1111/j.1423-0410.1987.tb03030.x

    authors: Zupańska B,Brojer E,Thomson EE,Merry AH,Seyfried H

    更新日期:1987-01-01 00:00:00

  • External quality assessment of human neutrophil antigen (HNA)-specific antibody detection and HNA genotyping from 2000 to 2012.

    abstract::Since 2000, Quality Assurance (QA) exercises for the detection and identification of granulocyte antibodies and DNA typing for human neutrophil antigens (HNA) have been distributed within the International Granulocyte Immunobiology Workshops, which are linked to International Society of Blood Transfusion. The exercise...

    journal_title:Vox sanguinis

    pub_type: 杂志文章

    doi:10.1111/vox.12041

    authors: Lucas G,Porcelijn L,Fung YL,Green F,Reil A,Hopkins M,Schuller R,Green A,de Haas M,Bux J

    更新日期:2013-10-01 00:00:00

  • Evaluation of a universal point-of-issue assay for bacterial detection in buffy coat platelet components.

    abstract::Bacterial contamination of platelet concentrates poses a major post-transfusion infectious risk. This study was aimed at evaluating the efficacy of the BacTx(®) assay (Immunetics Inc.) for bacterial detection in leucocyte-reduced buffy coat platelet pools and for its sensitivity in detecting clinical isolates, includi...

    journal_title:Vox sanguinis

    pub_type: 杂志文章

    doi:10.1111/vox.12148

    authors: Ramirez-Arcos S,Kou Y,Perkins H

    更新日期:2014-08-01 00:00:00

  • A new liquid, intravenous immunoglobulin product (IGIV 10%) highly purified by a state-of-the-art process.

    abstract:BACKGROUND AND OBJECTIVES:The ultimate goal was to generate an industrial-scale process suitable to produce a high-yield, safe and stable immunoglobulin G (IgG) preparation for intravenous administration, which is ready to use for customer convenience. This new liquid 10% IgG preparation (IGIV 10%) was compared to Gamm...

    journal_title:Vox sanguinis

    pub_type: 杂志文章

    doi:10.1111/j.1423-0410.2006.00846.x

    authors: Teschner W,Butterweck HA,Auer W,Muchitsch EM,Weber A,Liu SL,Wah PS,Schwarz HP

    更新日期:2007-01-01 00:00:00

  • Platelet-rich plasma and platelet gel preparation using Plateltex.

    abstract:BACKGROUND:The platelet gel is made by embedding concentrate platelets within a semisolid (gel) network of polymerized fibrin. It is believed that this blood component will be used more and more in the treatment of several clinical conditions and as an adjunctive material in tissue engineering. Several systems are avai...

    journal_title:Vox sanguinis

    pub_type: 杂志文章

    doi:10.1111/j.1423-0410.2007.01027.x

    authors: Mazzucco L,Balbo V,Cattana E,Borzini P

    更新日期:2008-04-01 00:00:00

  • Development of white blood cell fragments, during the preparation and storage of platelet concentrates, as measured by using real-time polymerase chain reaction.

    abstract:BACKGROUND AND OBJECTIVES:White blood cell (WBC) fragments may cause human leucocyte antigen (HLA) immunization in recipients. We investigated the occurrence and production of WBC fragments in platelet concentrates (PCs) and plasma units, during storage and filtration, by using real-time polymerase chain reaction (PCR)...

    journal_title:Vox sanguinis

    pub_type: 杂志文章

    doi:10.1111/j.1423-0410.2004.00576.x

    authors: Dijkstra-Tiekstra MJ,van der Schoot CE,Pietersz RN,Huijgens PC,van der Meer PF,Reesink HW

    更新日期:2004-11-01 00:00:00

  • Identification and removal of polymer- and aggregate-forming proteins in human plasma albumin preparations.

    abstract::The presence of the glycoproteins haptoglobin and hemopexin in human plasma albumin (HPA) solutions were demonstrated to be responsible for the formation of polymers and aggregates during heat treatment for 10 h at 60 degrees C. Apart from haptoglobin and hemopexin three other contaminating proteins were identified as...

    journal_title:Vox sanguinis

    pub_type: 杂志文章

    doi:10.1111/j.1423-0410.1994.tb01646.x

    authors: Jensen LB,Dam J,Teisner B

    更新日期:1994-01-01 00:00:00

  • Evaluation of two different protocols for peripheral blood stem cell collection with the Fresenius AS 104 blood cell separator.

    abstract:OBJECTIVES:Reconstitution of hematopoiesis by means of peripheral blood stem cells is a valid alternative to autologous bone marrow transplantation. The aim of this investigation was to increase the efficiency of collection of circulating blood progenitor cells and to obtain a purer product for transplant. METHODS:We ...

    journal_title:Vox sanguinis

    pub_type: 临床试验,杂志文章

    doi:10.1046/j.1423-0410.1997.7340230.x

    authors: Menichella G,Lai M,Pierelli L,Vittori M,Serafini R,Ciarli M,Foddai ML,Salerno G,Sica S,Scambia G,Leone G,Bizzi B

    更新日期:1997-01-01 00:00:00

  • A single unit of transfused allogeneic blood increases postoperative infections.

    abstract::The aim of this prospective study was to evaluate if there was a statistical correlation between allogeneic blood transfusion and postoperative infections, and if this could have a dose-dependent pattern. The evaluation was based on multiple logistic and receiver operating characteristics (ROC) curve analyses. On hosp...

    journal_title:Vox sanguinis

    pub_type: 杂志文章

    doi:10.1046/j.1423-0410.1996.7130170.x

    authors: Vignali A,Braga M,Gianotti L,Radaelli G,Gentilini O,Russo A,Di Carlo V

    更新日期:1996-01-01 00:00:00

  • Red-blood-cell alloimmunization and female sex predict the presence of HLA antibodies in patients undergoing liver transplant.

    abstract:BACKGROUND:Refractoriness to platelet transfusion because of HLA antibodies can jeopardize the outcome of patients who require platelet support during surgery. As routine screening for HLA antibodies would pose a huge burden on presurgical work-ups, a stratification of patients according to the risk of having HLA antib...

    journal_title:Vox sanguinis

    pub_type: 杂志文章

    doi:10.1111/j.1423-0410.2010.01347.x

    authors: Sanz C,Ghita G,Franquet C,Martínez I,Pereira A

    更新日期:2010-10-01 00:00:00

  • Cord blood banking and transplant in Europe. Eurocord.

    abstract::The number of cord blood transplants has been increasing very quickly with more than 250 cases reported to Eurocord Registry and more than 500 patients transplanted via the New York Cord Blood Bank. Cord blood transplants have been performed either with related or unrelated cord blood. Several cord blood banks establi...

    journal_title:Vox sanguinis

    pub_type: 杂志文章,评审

    doi:10.1111/j.1423-0410.1998.tb05403.x

    authors: Gluckman E,Rocha V,Chastang C

    更新日期:1998-01-01 00:00:00

  • Lectin studies. III. A survey of phytohemagglutinins: interaction of lectins with erythrocytes of ten vertebrate species.

    abstract::Extracts of seeds from 79 plant species were tested for agglutinating and lytic activity with the red cells of ten vertebrate species (man, monkey, rabbit, rat, goat, sheep, guinea pig, horse, fowl, dog). Of these extracts, 24 were hemagglutinating and 15 were hemolytic. The results suggest that some lectins can serve...

    journal_title:Vox sanguinis

    pub_type: 杂志文章

    doi:10.1111/j.1423-0410.1978.tb02928.x

    authors: Bhalla V,Gaur V,Bhatia K

    更新日期:1978-01-01 00:00:00

  • Immunochemical characterization of the Rh CW antigen using human monoclonal antibodies.

    abstract:BACKGROUND AND OBJECTIVES:Human monoclonal antibodies have been produced against various Rh antigens. We report here the production of another one against Rh C and the first such antibody against Rh CW. MATERIALS AND METHODS:Two heterohybridomas secreting IgG human monoclonal antibodies against Rh CW and Rh C (MS-353 ...

    journal_title:Vox sanguinis

    pub_type: 杂志文章

    doi:10.1046/j.1423-0410.1997.7330174.x

    authors: Thorpe SJ,Boult CE,Thompson KM

    更新日期:1997-01-01 00:00:00

  • Hemostasis testing during massive blood replacement. A study of 172 cases.

    abstract::A 24-hours service was organized to study changes in the hemostatic system in surgical patients undergoing massive transfusion for excessive bleeding during operation or in the early postoperative period. Hemostasis tests gave normal results in only 12(7%) of the 172 patients, while in the remaining 160(93%) one or mo...

    journal_title:Vox sanguinis

    pub_type: 杂志文章

    doi:10.1111/j.1423-0410.1982.tb01080.x

    authors: Mannucci PM,Federici AB,Sirchia G

    更新日期:1982-03-01 00:00:00

  • A new therapeutic antibody removal method using antigen-positive red cells. II. Application to a P-incompatible pregnant woman.

    abstract::Therapeutic antibody removal using antigen-positive red cells was applied to a pregnant woman who has high titered IgG anti-P and had lost all of 4 previous fetuses in P-incompatible pregnancy. The treatment was commenced at the 6th week of gestation and was intensively performed 93 times up to the 35th week. The volu...

    journal_title:Vox sanguinis

    pub_type: 杂志文章

    doi:10.1111/j.1423-0410.1984.tb01589.x

    authors: Yoshida H,Ito K,Emi N,Kanzaki H,Matsuura S

    更新日期:1984-01-01 00:00:00

  • Estimation of the number of binding sites for a murine monoclonal anti-Lub on human erythrocytes.

    abstract::The number of Lub antigen sites on the human erythrocyte membrane, as recognised by the murine monoclonal antibody BRIC-108, has been determined. The number of antibody molecules bound per cell on one example of cells of the phenotype Lu(a-b-) with recessive inheritance was an average of around 200 in replicate determ...

    journal_title:Vox sanguinis

    pub_type: 杂志文章

    doi:10.1111/j.1423-0410.1987.tb04915.x

    authors: Merry AH,Gardner B,Parsons SF,Anstee DJ

    更新日期:1987-01-01 00:00:00

  • A new low-frequency antigen BOW (Bowyer).

    abstract::BOW is a 'new' low-frequency red-cell antigen, detected in 2 unrelated English blood donors, that is sensitive to alpha-chymotrypsin and pronase. Anti-BOW is present in many polyspecific reagents used to define low-frequency antigens. Red-cell groups of the proposita, R.B., and her family show that the BOW blood group...

    journal_title:Vox sanguinis

    pub_type: 杂志文章

    doi:10.1111/j.1423-0410.1988.tb04705.x

    authors: Chaves MA,Leak MR,Poole J,Giles CM

    更新日期:1988-01-01 00:00:00

  • Paediatric transfusion.

    abstract::The requirements of children undergoing transfusion should be considered as a distinct entity from those of adults. Neonates are particularly vulnerable and there have been concerns over infective or toxic risks to this group. Neonates may also have more acute side-effects as a result of their small blood volume. Most...

    journal_title:Vox sanguinis

    pub_type: 杂志文章,评审

    doi:10.1111/j.1423-0410.2006.00722.x

    authors: New HV

    更新日期:2006-01-01 00:00:00